Restriction map for pF1KE2364
 (Circular) (Six-base) MAPSORT of: pF1KE2364.seq  Check: 8444  from: 1  to: 3818



pF1KE2364  3818 bp

 With 182 enzymes: SgfI * 

                        August 4, 2011 11:52  ..

AatII G_ACGT'C

Cuts at:    262     262 
   Size:       3818
 
AccI GT'mk_AC

Cuts at:    856     856 
   Size:       3818
 
Acc65I G'GTAC_C

Cuts at:    834     834 
   Size:       3818
 
AclI AA'CG_TT

Cuts at:   3658    3658 
   Size:       3818
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    250     465    1643    2075    2822    3090     250 
   Size:        215    1178     432     747     268     978

  Fragments arranged by size: 

                1178     978     747     432     268     215
 
AflIII A'CryG_T

Cuts at:    744    1170    2274    3251     744 
   Size:        426    1104     977    1311

  Fragments arranged by size: 

                1311    1104     977     426
 
AgeI A'CCGG_T

Cuts at:   2206    2206 
   Size:       3818
 
AlwNI CAG_nnn'CTG

Cuts at:    243     576    1123    2690     243 
   Size:        333     547    1567    1371

  Fragments arranged by size: 

                1567    1371     547     333
 
ApaLI G'TGCA_C

Cuts at:    622    2588     622 
   Size:       1966    1852
 
ApoI r'AATT_y

Cuts at:    822    3171    3427     822 
   Size:       2349     256    1213

  Fragments arranged by size: 

                2349    1213     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3818
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3818
 
AvaI C'yCGr_G

Cuts at:    375     838     375 
   Size:        463    3355
 
BamHI G'GATC_C

Cuts at:    843     843 
   Size:       3818
 
BanI G'GyrC_C

Cuts at:    292     552     666     834    1248    1496    1531     292 
   Size:        260     114     168     414     248      35    2579

  Fragments arranged by size: 

                2579     414     260     248     168     114      35
 
BanII G_rGCy'C

Cuts at:    832    1862     832 
   Size:       1030    2788
 
BbeI G_GCGC'C

Cuts at:    296     556     670    1252    1500     296 
   Size:        260     114     582     248    2614

  Fragments arranged by size: 

                2614     582     260     248     114
 
BbsI GAAGACnn'nnnn_

Cuts at:    492     747     492 
   Size:        255    3563
 
BciVI GTATCCnnnnn_n'

Cuts at:   1716    2477    3818    1716 
   Size:        761    1341    1716

  Fragments arranged by size: 

                1716    1341     761
 
BclI T'GATC_A

Cuts at:   1338    1338 
   Size:       3818
 
BglII A'GATC_T

Cuts at:   1333    3370    1333 
   Size:       2037    1781
 
BlpI GC'TnA_GC

Cuts at:    930     930 
   Size:       3818
 
Bme1580I G_kGCm'C

Cuts at:    626    1443    1536    2592     626 
   Size:        817      93    1056    1852

  Fragments arranged by size: 

                1852    1056     817      93
 
BmgBI CAC'GTC

Cuts at:    745     745 
   Size:       3818
 
BmrI ACTGGGnnnn_n'

Cuts at:   1030    1111    1446    3590    1030 
   Size:         81     335    2144    1258

  Fragments arranged by size: 

                2144    1258     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2185    2185 
   Size:       3818
 
Bpu10I CC'TnA_GC

Cuts at:    412     460     412 
   Size:         48    3770
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    994    1873    2365    2663     994 
   Size:        879     492     298    2149

  Fragments arranged by size: 

                2149     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3522    3522 
   Size:       3818
 
BsaAI yAC'GTr

Cuts at:   1171    1171 
   Size:       3818
 
BsaBI GATnn'nnATC

Cuts at:      6    1332    3369       6 
   Size:       1326    2037     455

  Fragments arranged by size: 

                2037    1326     455
 
BsaHI Gr'CG_yC

Cuts at:    259     293     526     553     667    1249    1497    2199    3695 
   Size:         34     233      27     114     582     248     702    1496

Cuts at:   3695     259 
   Size:        382

  Fragments arranged by size: 

                1496     702     582     382     248     233     114      34
                  27
 
BsaWI w'CCGG_w

Cuts at:   1007    1528    2206    2480    2627    3038    1007 
   Size:        521     678     274     147     411    1787

  Fragments arranged by size: 

                1787     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    217     247     217 
   Size:         30    3788
 
BseYI C'CCAG_C

Cuts at:    365     790    1243    2001    2578    3074     365 
   Size:        425     453     758     577     496    1109

  Fragments arranged by size: 

                1109     758     577     496     453     425
 
BsiEI CG_ry'CG

Cuts at:    104     350    1360    1406    2614     104 
   Size:        246    1010      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1010     246      46
 
BsiHKAI G_wGCw'C

Cuts at:    626     832    1610    1800    2592     626 
   Size:        206     778     190     792    1852

  Fragments arranged by size: 

                1852     792     778     206     190
 
Bsp1286I G_dGCh'C

Cuts at:    626     832    1443    1536    1610    1800    1862    2592     626 
   Size:        206     611      93      74     190      62     730    1852

  Fragments arranged by size: 

                1852     730     611     206     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3038    3038 
   Size:       3818
 
BspHI T'CATG_A

Cuts at:   2994    2994 
   Size:       3818
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    868    1082    1384    1765     868 
   Size:        214     302     381    2921

  Fragments arranged by size: 

                2921     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2110    2164    3648    3813      50 
   Size:       2060      54    1484     165      55

  Fragments arranged by size: 

                2060    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1730    1730 
   Size:       3818
 
BsrFI r'CCGG_y

Cuts at:    495     549     599    1816    1997    2206     495 
   Size:         54      50    1217     181     209    2107

  Fragments arranged by size: 

                2107    1217     209     181      54      50
 
BssSI C'ACGA_G

Cuts at:    270     627    2089    2447     270 
   Size:        357    1462     358    1641

  Fragments arranged by size: 

                1641    1462     358     357
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1195    1195 
   Size:       3818
 
BstBI TT'CG_AA

Cuts at:   2179    2179 
   Size:       3818
 
BstYI r'GATC_y

Cuts at:      1     843    1310    1333    1668    1914    2926    3012    3024 
   Size:        842     467      23     335     246    1012      86      12

Cuts at:   3024    3370       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     842     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3818
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    546    1768    1949    2112     546 
   Size:       1222     181     163    2252

  Fragments arranged by size: 

                2252    1222     181     163
 
BtsI GCAGTG_nn'

Cuts at:    626    1061     626 
   Size:        435    3383
 
DraI TTT'AAA

Cuts at:    817     817 
   Size:       3818
 
DraIII CAC_nnn'GTG

Cuts at:    492     492 
   Size:       3818
 
DrdI GACnn_nn'nnGTC

Cuts at:    400    1524    2382     400 
   Size:       1124     858    1836

  Fragments arranged by size: 

                1836    1124     858
 
EaeI y'GGCC_r

Cuts at:    347     601     674    1403    1577    1968    1995    3340     347 
   Size:        254      73     729     174     391      27    1345     825

  Fragments arranged by size: 

                1345     825     729     391     254     174      73      27
            
 
EagI C'GGCC_G

Cuts at:    347    1403     347 
   Size:       1056    2762
 
EarI CTCTTCn'nnn_

Cuts at:    283    1841    2051     283 
   Size:       1558     210    2050

  Fragments arranged by size: 

                2050    1558     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    412    2026    2336    2482    3628     412 
   Size:       1614     310     146    1146     602

  Fragments arranged by size: 

                1614    1146     602     310     146
 
EcoICRI GAG'CTC

Cuts at:    830     830 
   Size:       3818
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    250     465    1643    2075    2822    3090     250 
   Size:        215    1178     432     747     268     978

  Fragments arranged by size: 

                1178     978     747     432     268     215
 
EcoO109I rG'GnC_Cy

Cuts at:    690     957     690 
   Size:        267    3551
 
EcoRI G'AATT_C

Cuts at:    822     822 
   Size:       3818
 
EcoRV GAT'ATC

Cuts at:    654     654 
   Size:       3818
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1837    1837 
   Size:       3818
 
FspI TGC'GCA

Cuts at:   1599    1599 
   Size:       3818
 
HaeII r_GCGC'y

Cuts at:    296     535     556     670    1252    1500    2522    3501     296 
   Size:        239      21     114     582     248    1022     979     613

  Fragments arranged by size: 

                1022     979     613     582     248     239     114      21
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1660    1690    1692    1722    1660 
   Size:         30       2      30    3756

  Fragments arranged by size: 

                3756      30      30       2
 
HincII GTy'rAC

Cuts at:    857     857 
   Size:       3818
 
KasI G'GCGC_C

Cuts at:    292     552     666    1248    1496     292 
   Size:        260     114     582     248    2614

  Fragments arranged by size: 

                2614     582     260     248     114
 
KpnI G_GTAC'C

Cuts at:    838     838 
   Size:       3818
 
MluI A'CGCG_T

Cuts at:   3251    3251 
   Size:       3818
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    275    2489    2673     275 
   Size:       2214     184    1420

  Fragments arranged by size: 

                2214    1420     184
 
MscI TGG'CCA

Cuts at:   1579    1579 
   Size:       3818
 
MslI CAynn'nnrTG

Cuts at:   1807    1934    1807 
   Size:        127    3691
 
MspA1I CmG'CkG

Cuts at:    141     192     369     607     929    1243    1603    2616    2861 
   Size:         51     177     238     322     314     360    1013     245

Cuts at:   2861     141 
   Size:       1098

  Fragments arranged by size: 

                1098    1013     360     322     314     245     238     177
                  51
 
NaeI GCC'GGC

Cuts at:    601    1999     601 
   Size:       1398    2420
 
NarI GG'CG_CC

Cuts at:    293     553     667    1249    1497     293 
   Size:        260     114     582     248    2614

  Fragments arranged by size: 

                2614     582     260     248     114
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3818
 
NgoMIV G'CCGG_C

Cuts at:    599    1997     599 
   Size:       1398    2420
 
NspI r_CATG'y

Cuts at:    871    1371    2278     871 
   Size:        500     907    2411

  Fragments arranged by size: 

                2411     907     500
 
PciI A'CATG_T

Cuts at:   2274    2274 
   Size:       3818
 
PmeI GTTT'AAAC

Cuts at:    817     817 
   Size:       3818
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2957    2989    2957 
   Size:         32    3786
 
PpuMI rG'GwC_Cy

Cuts at:    690     690 
   Size:       3818
 
PstI C_TGCA'G

Cuts at:    865    1550     865 
   Size:        685    3133
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3818
 
PvuII CAG'CTG

Cuts at:   1243    1603    1243 
   Size:        360    3458
 
RsrII CG'GwC_CG

Cuts at:   2013    2013 
   Size:       3818
 
SacI G_AGCT'C

Cuts at:    832     832 
   Size:       3818
 
SalI G'TCGA_C

Cuts at:    855     855 
   Size:       3818
 
SapI GCTCTTCn'nnn_

Cuts at:    283    1841    2051     283 
   Size:       1558     210    2050

  Fragments arranged by size: 

                2050    1558     210
 
SbfI CC_TGCA'GG

Cuts at:    865     865 
   Size:       3818
 
ScaI AGT'ACT

Cuts at:   3335    3335 
   Size:       3818
 
SfcI C'TryA_G

Cuts at:     33     861    1546    2539    2730      33 
   Size:        828     685     993     191    1121

  Fragments arranged by size: 

                1121     993     828     685     191
 
SfoI GGC'GCC

Cuts at:    294     554     668    1250    1498     294 
   Size:        260     114     582     248    2614

  Fragments arranged by size: 

                2614     582     260     248     114
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3818
 
SgrAI Cr'CCGG_yG

Cuts at:    549     549 
   Size:       3818
 
SmaI CCC'GGG

Cuts at:    840     840 
   Size:       3818
 
SmlI C'TyrA_G

Cuts at:    973    1888    2380    2642     973 
   Size:        915     492     262    2149

  Fragments arranged by size: 

                2149     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3818
 
SphI G_CATG'C

Cuts at:    871    1371     871 
   Size:        500    3318
 
StyI C'CwwG_G

Cuts at:    107     341     671     952    1306     107 
   Size:        234     330     281     354    2619

  Fragments arranged by size: 

                2619     354     330     281     234
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    379    1401    1534    1993    2202     379 
   Size:       1022     133     459     209    1995

  Fragments arranged by size: 

                1995    1022     459     209     133
 
TatI w'GTAC_w

Cuts at:    476    3333     476 
   Size:       2857     961
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    217     249    3335    3367     217 
   Size:         32    3086      32     668

  Fragments arranged by size: 

                3086     668      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1615    1615 
   Size:       3818
 
XbaI T'CTAG_A

Cuts at:    849     849 
   Size:       3818
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1313    1313 
   Size:       3818
 
XmaI C'CCGG_G

Cuts at:    838     838 
   Size:       3818
 
ZraI GAC'GTC

Cuts at:    260     260 
   Size:       3818
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
     BtgI    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
    SgrAI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI     BglI     BmtI
     BpmI    BseRI     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII
   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI    EcoNI     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI
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