Restriction map for pF1KE2364
 (Linear) (Six-base) MAPSORT of: KIEE2364.seq  Check: 3674  from: 1  to: 705



KIEE2364.orf

 With 182 enzymes: SgfI * 

                        August 4, 2011 11:52  ..

AatII G_ACGT'C

Cuts at:      0     154     705 
   Size:        154     551
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     357     705 
   Size:        142     215     348

  Fragments arranged by size: 

                 348     215     142
 
AflIII A'CryG_T

Cuts at:      0     636     705 
   Size:        636      69
 
AlwNI CAG_nnn'CTG

Cuts at:      0     135     468     705 
   Size:        135     333     237

  Fragments arranged by size: 

                 333     237     135
 
ApaLI G'TGCA_C

Cuts at:      0     514     705 
   Size:        514     191
 
AvaI C'yCGr_G

Cuts at:      0     267     705 
   Size:        267     438
 
BanI G'GyrC_C

Cuts at:      0     184     444     558     705 
   Size:        184     260     114     147

  Fragments arranged by size: 

                 260     184     147     114
 
BbeI G_GCGC'C

Cuts at:      0     188     448     562     705 
   Size:        188     260     114     143

  Fragments arranged by size: 

                 260     188     143     114
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     384     639     705 
   Size:        384     255      66

  Fragments arranged by size: 

                 384     255      66
 
Bme1580I G_kGCm'C

Cuts at:      0     518     705 
   Size:        518     187
 
BmgBI CAC'GTC

Cuts at:      0     637     705 
   Size:        637      68
 
Bpu10I CC'TnA_GC

Cuts at:      0     304     352     705 
   Size:        304      48     353

  Fragments arranged by size: 

                 353     304      48
 
BsaHI Gr'CG_yC

Cuts at:      0     151     185     418     445     559     705 
   Size:        151      34     233      27     114     146

  Fragments arranged by size: 

                 233     151     146     114      34      27
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     109     139     705 
   Size:        109      30     566

  Fragments arranged by size: 

                 566     109      30
 
BseYI C'CCAG_C

Cuts at:      0     257     682     705 
   Size:        257     425      23

  Fragments arranged by size: 

                 425     257      23
 
BsiEI CG_ry'CG

Cuts at:      0     242     705 
   Size:        242     463
 
BsiHKAI G_wGCw'C

Cuts at:      0     518     705 
   Size:        518     187
 
Bsp1286I G_dGCh'C

Cuts at:      0     518     705 
   Size:        518     187
 
BsrFI r'CCGG_y

Cuts at:      0     387     441     491     705 
   Size:        387      54      50     214

  Fragments arranged by size: 

                 387     214      54      50
 
BssSI C'ACGA_G

Cuts at:      0     162     519     705 
   Size:        162     357     186

  Fragments arranged by size: 

                 357     186     162
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     438     705 
   Size:        438     267
 
BtsI GCAGTG_nn'

Cuts at:      0     518     705 
   Size:        518     187
 
DraIII CAC_nnn'GTG

Cuts at:      0     384     705 
   Size:        384     321
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     292     705 
   Size:        292     413
 
EaeI y'GGCC_r

Cuts at:      0     239     493     566     705 
   Size:        239     254      73     139

  Fragments arranged by size: 

                 254     239     139      73
 
EagI C'GGCC_G

Cuts at:      0     239     705 
   Size:        239     466
 
EarI CTCTTCn'nnn_

Cuts at:      0     175     705 
   Size:        175     530
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     304     705 
   Size:        304     401
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     357     705 
   Size:        142     215     348

  Fragments arranged by size: 

                 348     215     142
 
EcoO109I rG'GnC_Cy

Cuts at:      0     582     705 
   Size:        582     123
 
EcoRV GAT'ATC

Cuts at:      0     546     705 
   Size:        546     159
 
HaeII r_GCGC'y

Cuts at:      0     188     427     448     562     705 
   Size:        188     239      21     114     143

  Fragments arranged by size: 

                 239     188     143     114      21
 
KasI G'GCGC_C

Cuts at:      0     184     444     558     705 
   Size:        184     260     114     147

  Fragments arranged by size: 

                 260     184     147     114
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     705 
   Size:        167     538
 
MspA1I CmG'CkG

Cuts at:      0      33      84     261     499     705 
   Size:         33      51     177     238     206

  Fragments arranged by size: 

                 238     206     177      51      33
 
NaeI GCC'GGC

Cuts at:      0     493     705 
   Size:        493     212
 
NarI GG'CG_CC

Cuts at:      0     185     445     559     705 
   Size:        185     260     114     146

  Fragments arranged by size: 

                 260     185     146     114
 
NgoMIV G'CCGG_C

Cuts at:      0     491     705 
   Size:        491     214
 
PpuMI rG'GwC_Cy

Cuts at:      0     582     705 
   Size:        582     123
 
SapI GCTCTTCn'nnn_

Cuts at:      0     175     705 
   Size:        175     530
 
SfoI GGC'GCC

Cuts at:      0     186     446     560     705 
   Size:        186     260     114     145

  Fragments arranged by size: 

                 260     186     145     114
 
SgrAI Cr'CCGG_yG

Cuts at:      0     441     705 
   Size:        441     264
 
StyI C'CwwG_G

Cuts at:      0     233     563     705 
   Size:        233     330     142

  Fragments arranged by size: 

                 330     233     142
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     271     705 
   Size:        271     434
 
TatI w'GTAC_w

Cuts at:      0     368     705 
   Size:        368     337
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     109     141     705 
   Size:        109      32     564

  Fragments arranged by size: 

                 564     109      32
 
ZraI GAC'GTC

Cuts at:      0     152     705 
   Size:        152     553
 

 Enzymes that do cut:

    AatII     AcuI   AflIII    AlwNI    ApaLI     AvaI     BanI     BbeI
     BbsI Bme1580I    BmgBI   Bpu10I    BsaHI    BsaXI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BsrFI    BssSI    BtgZI     BtsI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRV    HaeII
     KasI     MmeI   MspA1I     NaeI     NarI   NgoMIV    PpuMI     SapI
     SfoI    SgrAI     StyI    TaqII     TatI     TstI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI     BmrI     BmtI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BseRI     BsgI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI     ClaI    CspCI     DraI  EcoICRI    EcoNI    EcoRI     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MscI     MslI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI
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