Restriction map for pF1KB8852
 (Circular) (Six-base) MAPSORT of: pF1KB8852.seq  Check: 9347  from: 1  to: 3338



pF1KB8852  3338 bp

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AccI GT'mk_AC

Cuts at:    376     376 
   Size:       3338
 
Acc65I G'GTAC_C

Cuts at:    354     354 
   Size:       3338
 
AclI AA'CG_TT

Cuts at:   3178    3178 
   Size:       3338
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1595    2342    2610    1163 
   Size:        432     747     268    1891

  Fragments arranged by size: 

                1891     747     432     268
 
AflII C'TTAA_G

Cuts at:    175     175 
   Size:       3338
 
AflIII A'CryG_T

Cuts at:    690    1794    2771     690 
   Size:       1104     977    1257

  Fragments arranged by size: 

                1257    1104     977
 
AgeI A'CCGG_T

Cuts at:   1726    1726 
   Size:       3338
 
AleI CACnn'nnGTG

Cuts at:    254     277     254 
   Size:         23    3315
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    298     330     298 
   Size:         32    3306
 
AlwNI CAG_nnn'CTG

Cuts at:    643    2210     643 
   Size:       1567    1771
 
ApaLI G'TGCA_C

Cuts at:   2108    2108 
   Size:       3338
 
ApoI r'AATT_y

Cuts at:    342    2691    2947     342 
   Size:       2349     256     733

  Fragments arranged by size: 

                2349     733     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3338
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3338
 
AvaI C'yCGr_G

Cuts at:    358     358 
   Size:       3338
 
BamHI G'GATC_C

Cuts at:    261     363     261 
   Size:        102    3236
 
BanI G'GyrC_C

Cuts at:    354     768    1016    1051     354 
   Size:        414     248      35    2641

  Fragments arranged by size: 

                2641     414     248      35
 
BanII G_rGCy'C

Cuts at:    352    1382     352 
   Size:       1030    2308
 
BbeI G_GCGC'C

Cuts at:    772    1020     772 
   Size:        248    3090
 
BciVI GTATCCnnnnn_n'

Cuts at:   1236    1997    3338    1236 
   Size:        761    1341    1236

  Fragments arranged by size: 

                1341    1236     761
 
BclI T'GATC_A

Cuts at:    858     858 
   Size:       3338
 
BglII A'GATC_T

Cuts at:    853    2890     853 
   Size:       2037    1301
 
BlpI GC'TnA_GC

Cuts at:    450     450 
   Size:       3338
 
Bme1580I G_kGCm'C

Cuts at:    284     963    1056    2112     284 
   Size:        679      93    1056    1510

  Fragments arranged by size: 

                1510    1056     679      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    550     631     966    3110     550 
   Size:         81     335    2144     778

  Fragments arranged by size: 

                2144     778     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1705    1705 
   Size:       3338
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    514    1393    1885    2183     514 
   Size:        879     492     298    1669

  Fragments arranged by size: 

                1669     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    112    3042     112 
   Size:       2930     408
 
BsaAI yAC'GTr

Cuts at:    691     691 
   Size:       3338
 
BsaBI GATnn'nnATC

Cuts at:      6     852    2889       6 
   Size:        846    2037     455

  Fragments arranged by size: 

                2037     846     455
 
BsaHI Gr'CG_yC

Cuts at:    769    1017    1719    3215     769 
   Size:        248     702    1496     892

  Fragments arranged by size: 

                1496     892     702     248
 
BsaWI w'CCGG_w

Cuts at:    527    1048    1726    2000    2147    2558     527 
   Size:        521     678     274     147     411    1307

  Fragments arranged by size: 

                1307     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    298     328     298 
   Size:         30    3308
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    319     319 
   Size:       3338
 
BseYI C'CCAG_C

Cuts at:    763    1521    2098    2594     763 
   Size:        758     577     496    1507

  Fragments arranged by size: 

                1507     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     880     926    2134     104 
   Size:        776      46    1208    1308

  Fragments arranged by size: 

                1308    1208     776      46
 
BsiHKAI G_wGCw'C

Cuts at:    327     352    1130    1320    2112     327 
   Size:         25     778     190     792    1553

  Fragments arranged by size: 

                1553     792     778     190      25
 
Bsp1286I G_dGCh'C

Cuts at:    284     327     352     963    1056    1130    1320    1382    2112 
   Size:         43      25     611      93      74     190      62     730

Cuts at:   2112     284 
   Size:       1510

  Fragments arranged by size: 

                1510     730     611     190      93      74      62      43
                  25
 
BspEI T'CCGG_A

Cuts at:   2558    2558 
   Size:       3338
 
BspHI T'CATG_A

Cuts at:   2514    2514 
   Size:       3338
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    388     602     904    1285     388 
   Size:        214     302     381    2441

  Fragments arranged by size: 

                2441     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1630    1684    3168    3333      50 
   Size:       1580      54    1484     165      55

  Fragments arranged by size: 

                1580    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1250    1250 
   Size:       3338
 
BsrFI r'CCGG_y

Cuts at:   1336    1517    1726    1336 
   Size:        181     209    2948

  Fragments arranged by size: 

                2948     209     181
 
BssSI C'ACGA_G

Cuts at:   1609    1967    1609 
   Size:        358    2980
 
BstAPI GCAn_nnn'nTGC

Cuts at:    715     715 
   Size:       3338
 
BstBI TT'CG_AA

Cuts at:   1699    1699 
   Size:       3338
 
BstYI r'GATC_y

Cuts at:      1     261     363     830     853    1188    1434    2446    2532 
   Size:        260     102     467      23     335     246    1012      86

Cuts at:   2532    2544    2890       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     260     246     102
                  86      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1288    1469    1632    1288 
   Size:        181     163    2994

  Fragments arranged by size: 

                2994     181     163
 
BtsI GCAGTG_nn'

Cuts at:    581     581 
   Size:       3338
 
DraI TTT'AAA

Cuts at:    337     337 
   Size:       3338
 
DrdI GACnn_nn'nnGTC

Cuts at:   1044    1902    1044 
   Size:        858    2480
 
EaeI y'GGCC_r

Cuts at:    923    1097    1488    1515    2860     923 
   Size:        174     391      27    1345    1401

  Fragments arranged by size: 

                1401    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:    923     923 
   Size:       3338
 
EarI CTCTTCn'nnn_

Cuts at:   1361    1571    1361 
   Size:        210    3128
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1546    1856    2002    3148    1546 
   Size:        310     146    1146    1736

  Fragments arranged by size: 

                1736    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    350     350 
   Size:       3338
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1595    2342    2610    1163 
   Size:        432     747     268    1891

  Fragments arranged by size: 

                1891     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    477     477 
   Size:       3338
 
EcoRI G'AATT_C

Cuts at:    342     342 
   Size:       3338
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    333    1357     333 
   Size:       1024    2314
 
FspI TGC'GCA

Cuts at:   1119    1119 
   Size:       3338
 
HaeII r_GCGC'y

Cuts at:    772    1020    2042    3021     772 
   Size:        248    1022     979    1089

  Fragments arranged by size: 

                1089    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1180    1210    1212    1242    1180 
   Size:         30       2      30    3276

  Fragments arranged by size: 

                3276      30      30       2
 
HincII GTy'rAC

Cuts at:    377     377 
   Size:       3338
 
KasI G'GCGC_C

Cuts at:    768    1016     768 
   Size:        248    3090
 
KpnI G_GTAC'C

Cuts at:    358     358 
   Size:       3338
 
MluI A'CGCG_T

Cuts at:   2771    2771 
   Size:       3338
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    178    2009    2193     178 
   Size:       1831     184    1323

  Fragments arranged by size: 

                1831    1323     184
 
MscI TGG'CCA

Cuts at:   1099    1099 
   Size:       3338
 
MslI CAynn'nnrTG

Cuts at:    254     277    1327    1454     254 
   Size:         23    1050     127    2138

  Fragments arranged by size: 

                2138    1050     127      23
 
MspA1I CmG'CkG

Cuts at:    449     763    1123    2136    2381     449 
   Size:        314     360    1013     245    1406

  Fragments arranged by size: 

                1406    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1519    1519 
   Size:       3338
 
NarI GG'CG_CC

Cuts at:    769    1017     769 
   Size:        248    3090
 
NgoMIV G'CCGG_C

Cuts at:   1517    1517 
   Size:       3338
 
NspI r_CATG'y

Cuts at:    391     891    1798     391 
   Size:        500     907    1931

  Fragments arranged by size: 

                1931     907     500
 
PciI A'CATG_T

Cuts at:   1794    1794 
   Size:       3338
 
PfoI T'CCnGG_A

Cuts at:    302     302 
   Size:       3338
 
PmeI GTTT'AAAC

Cuts at:    337     337 
   Size:       3338
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    298     330    2477    2509     298 
   Size:         32    2147      32    1127

  Fragments arranged by size: 

                2147    1127      32      32
 
PstI C_TGCA'G

Cuts at:    385    1070     385 
   Size:        685    2653
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3338
 
PvuII CAG'CTG

Cuts at:    763    1123     763 
   Size:        360    2978
 
RsrII CG'GwC_CG

Cuts at:   1533    1533 
   Size:       3338
 
SacI G_AGCT'C

Cuts at:    352     352 
   Size:       3338
 
SalI G'TCGA_C

Cuts at:    375     375 
   Size:       3338
 
SapI GCTCTTCn'nnn_

Cuts at:   1361    1571    1361 
   Size:        210    3128
 
SbfI CC_TGCA'GG

Cuts at:    385     385 
   Size:       3338
 
ScaI AGT'ACT

Cuts at:   2855    2855 
   Size:       3338
 
SfcI C'TryA_G

Cuts at:     33     381    1066    2059    2250      33 
   Size:        348     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     348     191
 
SfoI GGC'GCC

Cuts at:    770    1018     770 
   Size:        248    3090
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3338
 
SmaI CCC'GGG

Cuts at:    360     360 
   Size:       3338
 
SmlI C'TyrA_G

Cuts at:    175     493    1408    1900    2162     175 
   Size:        318     915     492     262    1351

  Fragments arranged by size: 

                1351     915     492     318     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3338
 
SphI G_CATG'C

Cuts at:    391     891     391 
   Size:        500    2838
 
StyI C'CwwG_G

Cuts at:    212     472     826     212 
   Size:        260     354    2724

  Fragments arranged by size: 

                2724     354     260
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    921    1054    1513    1722     921 
   Size:        133     459     209    2537

  Fragments arranged by size: 

                2537     459     209     133
 
TatI w'GTAC_w

Cuts at:   2853    2853 
   Size:       3338
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2855    2887    2855 
   Size:         32    3306
 
Tth111I GACn'n_nGTC

Cuts at:   1135    1135 
   Size:       3338
 
XbaI T'CTAG_A

Cuts at:    369     369 
   Size:       3338
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    833     833 
   Size:       3338
 
XmaI C'CCGG_G

Cuts at:    358     358 
   Size:       3338
 
XmnI GAAnn'nnTTC

Cuts at:    298     313     316     298 
   Size:         15       3    3320

  Fragments arranged by size: 

                3320      15       3
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AleI
     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NspI     PciI     PfoI     PmeI     PpiI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AlfI     ApaI     AscI    AvrII
     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI    BmgBI     BmtI
     BpmI   Bpu10I     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI
     SwaI     XhoI     ZraI
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