Restriction map for pF1KB5482
 (Circular) (Six-base) MAPSORT of: pF1KB5482.seq  Check: 8022  from: 1  to: 3848



pF1KB5482  3848 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AccI GT'mk_AC

Cuts at:    886     886 
   Size:       3848
 
Acc65I G'GTAC_C

Cuts at:    864     864 
   Size:       3848
 
AclI AA'CG_TT

Cuts at:   3688    3688 
   Size:       3848
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1673    2105    2852    3120    1673 
   Size:        432     747     268    2401

  Fragments arranged by size: 

                2401     747     432     268
 
AflII C'TTAA_G

Cuts at:    304     304 
   Size:       3848
 
AflIII A'CryG_T

Cuts at:   1200    2304    3281    1200 
   Size:       1104     977    1767

  Fragments arranged by size: 

                1767    1104     977
 
AgeI A'CCGG_T

Cuts at:   2236    2236 
   Size:       3848
 
AleI CACnn'nnGTG

Cuts at:    525     525 
   Size:       3848
 
AlwNI CAG_nnn'CTG

Cuts at:   1153    2720    1153 
   Size:       1567    2281
 
ApaLI G'TGCA_C

Cuts at:   2618    2618 
   Size:       3848
 
ApoI r'AATT_y

Cuts at:    532     852    3201    3457     532 
   Size:        320    2349     256     923

  Fragments arranged by size: 

                2349     923     320     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3848
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3848
 
AvaI C'yCGr_G

Cuts at:    248     868     248 
   Size:        620    3228
 
BamHI G'GATC_C

Cuts at:    873     873 
   Size:       3848
 
BanI G'GyrC_C

Cuts at:    379     864    1278    1526    1561     379 
   Size:        485     414     248      35    2666

  Fragments arranged by size: 

                2666     485     414     248      35
 
BanII G_rGCy'C

Cuts at:    862    1892     862 
   Size:       1030    2818
 
BbeI G_GCGC'C

Cuts at:   1282    1530    1282 
   Size:        248    3600
 
BbsI GAAGACnn'nnnn_

Cuts at:    294     294 
   Size:       3848
 
BbvCI CC'TCA_GC

Cuts at:    299     299 
   Size:       3848
 
BciVI GTATCCnnnnn_n'

Cuts at:    487    1746    2507    3848     487 
   Size:       1259     761    1341     487

  Fragments arranged by size: 

                1341    1259     761     487
 
BclI T'GATC_A

Cuts at:   1368    1368 
   Size:       3848
 
BglI GCCn_nnn'nGGC

Cuts at:    112     338     112 
   Size:        226    3622
 
BglII A'GATC_T

Cuts at:   1363    3400    1363 
   Size:       2037    1811
 
BlpI GC'TnA_GC

Cuts at:    960     960 
   Size:       3848
 
Bme1580I G_kGCm'C

Cuts at:   1473    1566    2622    1473 
   Size:         93    1056    2699

  Fragments arranged by size: 

                2699    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1060    1141    1476    3620    1060 
   Size:         81     335    2144    1288

  Fragments arranged by size: 

                2144    1288     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2215    2215 
   Size:       3848
 
Bpu10I CC'TnA_GC

Cuts at:    299     299 
   Size:       3848
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1024    1903    2395    2693    1024 
   Size:        879     492     298    2179

  Fragments arranged by size: 

                2179     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3552    3552 
   Size:       3848
 
BsaAI yAC'GTr

Cuts at:   1201    1201 
   Size:       3848
 
BsaBI GATnn'nnATC

Cuts at:      6    1362    3399       6 
   Size:       1356    2037     455

  Fragments arranged by size: 

                2037    1356     455
 
BsaHI Gr'CG_yC

Cuts at:   1279    1527    2229    3725    1279 
   Size:        248     702    1496    1402

  Fragments arranged by size: 

                1496    1402     702     248
 
BsaWI w'CCGG_w

Cuts at:   1037    1558    2236    2510    2657    3068    1037 
   Size:        521     678     274     147     411    1817

  Fragments arranged by size: 

                1817     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    199    1273    2031    2608    3104     199 
   Size:       1074     758     577     496     943

  Fragments arranged by size: 

                1074     943     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    718     718 
   Size:       3848
 
BsiEI CG_ry'CG

Cuts at:    104    1390    1436    2644     104 
   Size:       1286      46    1208    1308

  Fragments arranged by size: 

                1308    1286    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    862    1640    1830    2622     862 
   Size:        778     190     792    2088

  Fragments arranged by size: 

                2088     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    862    1473    1566    1640    1830    1892    2622     862 
   Size:        611      93      74     190      62     730    2088

  Fragments arranged by size: 

                2088     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3068    3068 
   Size:       3848
 
BspHI T'CATG_A

Cuts at:    644    3024     644 
   Size:       2380    1468
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    898    1112    1414    1795     898 
   Size:        214     302     381    2951

  Fragments arranged by size: 

                2951     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2140    2194    3678    3843      50 
   Size:       2090      54    1484     165      55

  Fragments arranged by size: 

                2090    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    485    1760     485 
   Size:       1275    2573
 
BsrFI r'CCGG_y

Cuts at:   1846    2027    2236    1846 
   Size:        181     209    3458

  Fragments arranged by size: 

                3458     209     181
 
BsrGI T'GTAC_A

Cuts at:    500     716     500 
   Size:        216    3632
 
BssSI C'ACGA_G

Cuts at:   2119    2477    2119 
   Size:        358    3490
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1225    1225 
   Size:       3848
 
BstBI TT'CG_AA

Cuts at:   2209    2209 
   Size:       3848
 
BstYI r'GATC_y

Cuts at:      1     873    1340    1363    1698    1944    2956    3042    3054 
   Size:        872     467      23     335     246    1012      86      12

Cuts at:   3054    3400       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     872     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3848
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1798    1979    2142    1798 
   Size:        181     163    3504

  Fragments arranged by size: 

                3504     181     163
 
BtsI GCAGTG_nn'

Cuts at:    730    1091     730 
   Size:        361    3487
 
DraI TTT'AAA

Cuts at:    847     847 
   Size:       3848
 
DrdI GACnn_nn'nnGTC

Cuts at:   1554    2412    1554 
   Size:        858    2990
 
EaeI y'GGCC_r

Cuts at:    710    1433    1607    1998    2025    3370     710 
   Size:        723     174     391      27    1345    1188

  Fragments arranged by size: 

                1345    1188     723     391     174      27
 
EagI C'GGCC_G

Cuts at:   1433    1433 
   Size:       3848
 
EarI CTCTTCn'nnn_

Cuts at:    139     265     268    1871    2081     139 
   Size:        126       3    1603     210    1906

  Fragments arranged by size: 

                1906    1603     210     126       3
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    376    2056    2366    2512    3658     376 
   Size:       1680     310     146    1146     566

  Fragments arranged by size: 

                1680    1146     566     310     146
 
EcoICRI GAG'CTC

Cuts at:    860     860 
   Size:       3848
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1673    2105    2852    3120    1673 
   Size:        432     747     268    2401

  Fragments arranged by size: 

                2401     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    770     987     770 
   Size:        217    3631
 
EcoRI G'AATT_C

Cuts at:    852     852 
   Size:       3848
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    489    1867     489 
   Size:       1378    2470
 
FspI TGC'GCA

Cuts at:   1629    1629 
   Size:       3848
 
HaeII r_GCGC'y

Cuts at:   1282    1530    2552    3531    1282 
   Size:        248    1022     979    1599

  Fragments arranged by size: 

                1599    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    573     605    1690    1720    1722    1752     573 
   Size:         32    1085      30       2      30    2669

  Fragments arranged by size: 

                2669    1085      32      30      30       2
 
HincII GTy'rAC

Cuts at:    887     887 
   Size:       3848
 
KasI G'GCGC_C

Cuts at:   1278    1526    1278 
   Size:        248    3600
 
KpnI G_GTAC'C

Cuts at:    868     868 
   Size:       3848
 
MluI A'CGCG_T

Cuts at:   3281    3281 
   Size:       3848
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    197    2519    2703     197 
   Size:       2322     184    1342

  Fragments arranged by size: 

                2322    1342     184
 
MscI TGG'CCA

Cuts at:    712    1609     712 
   Size:        897    2951
 
MslI CAynn'nnrTG

Cuts at:    424     525    1837    1964     424 
   Size:        101    1312     127    2308

  Fragments arranged by size: 

                2308    1312     127     101
 
MspA1I CmG'CkG

Cuts at:    218     354     959    1273    1633    2646    2891     218 
   Size:        136     605     314     360    1013     245    1175

  Fragments arranged by size: 

                1175    1013     605     360     314     245     136
 
NaeI GCC'GGC

Cuts at:   2029    2029 
   Size:       3848
 
NarI GG'CG_CC

Cuts at:   1279    1527    1279 
   Size:        248    3600
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3848
 
NgoMIV G'CCGG_C

Cuts at:   2027    2027 
   Size:       3848
 
NspI r_CATG'y

Cuts at:    901    1401    2308     901 
   Size:        500     907    2441

  Fragments arranged by size: 

                2441     907     500
 
PciI A'CATG_T

Cuts at:   2304    2304 
   Size:       3848
 
PmeI GTTT'AAAC

Cuts at:    847     847 
   Size:       3848
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2987    3019    2987 
   Size:         32    3816
 
PpuMI rG'GwC_Cy

Cuts at:    770     770 
   Size:       3848
 
PstI C_TGCA'G

Cuts at:    359     681     895    1580     359 
   Size:        322     214     685    2627

  Fragments arranged by size: 

                2627     685     322     214
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3848
 
PvuII CAG'CTG

Cuts at:    218     354    1273    1633     218 
   Size:        136     919     360    2433

  Fragments arranged by size: 

                2433     919     360     136
 
RsrII CG'GwC_CG

Cuts at:   2043    2043 
   Size:       3848
 
SacI G_AGCT'C

Cuts at:    862     862 
   Size:       3848
 
SalI G'TCGA_C

Cuts at:    885     885 
   Size:       3848
 
SanDI GG'GwC_CC

Cuts at:    770     770 
   Size:       3848
 
SapI GCTCTTCn'nnn_

Cuts at:   1871    2081    1871 
   Size:        210    3638
 
SbfI CC_TGCA'GG

Cuts at:    895     895 
   Size:       3848
 
ScaI AGT'ACT

Cuts at:   3365    3365 
   Size:       3848
 
SfcI C'TryA_G

Cuts at:     33     355     677     891    1576    2569    2760      33 
   Size:        322     322     214     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     322     322     214     191
 
SfoI GGC'GCC

Cuts at:   1280    1528    1280 
   Size:        248    3600
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3848
 
SmaI CCC'GGG

Cuts at:    250     870     250 
   Size:        620    3228
 
SmlI C'TyrA_G

Cuts at:    304    1003    1918    2410    2672     304 
   Size:        699     915     492     262    1480

  Fragments arranged by size: 

                1480     915     699     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3848
 
SphI G_CATG'C

Cuts at:    901    1401     901 
   Size:        500    3348
 
StyI C'CwwG_G

Cuts at:    107     450     982    1336     107 
   Size:        343     532     354    2619

  Fragments arranged by size: 

                2619     532     354     343
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1431    1564    2023    2232    1431 
   Size:        133     459     209    3047

  Fragments arranged by size: 

                3047     459     209     133
 
TatI w'GTAC_w

Cuts at:    470     500     716    3363     470 
   Size:         30     216    2647     955

  Fragments arranged by size: 

                2647     955     216      30
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3365    3397    3365 
   Size:         32    3816
 
Tth111I GACn'n_nGTC

Cuts at:   1645    1645 
   Size:       3848
 
XbaI T'CTAG_A

Cuts at:    879     879 
   Size:       3848
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    471    1343     471 
   Size:        872    2976
 
XmaI C'CCGG_G

Cuts at:    248     868     248 
   Size:        620    3228
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI     AleI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI
   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PciI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI     BcgI    BfrBI    BmgBI     BmtI     BpmI    BsaXI
    BseRI    BsiWI     BsmI    BsmBI   BssHII   BstEII    BstXI  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI
  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SexAI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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