Restriction map for pF1KE1090
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AgeI        AhdI        AocI        
Aor51HI     ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BbeI        BbvCI       BcgI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsaI        BsgI        BsiEI       BsiWI       
BsmI        BspDI       BspHI       BspLU11I    BsrBI       
BsrDI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FseI        FspI        
Hin1I       HindIII     HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MluNI       
MscI        MunI        MvnI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmeI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        Tth111I     XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AccIII       1730  TCCGGA               T^CCGGA
Asp700I       297  GAACTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          358  CCCGAG               C^[CT]CG[GA]G
BanII        1591  GAGCCC               G[GA]GC[CT]^C
BbrPI         100  CACGTG               CAC^GTG
BciVI         363  GTATCC               GTATCC(6/5)
BclI          104  TGATCA               T^GATCA
BglII         764  AGATCT               A^GATCT
BsaAI         100  CACGTG               [CT]AC^GT[GA]
BseAI        1730  TCCGGA               T^CCGGA
BsmBI          40  GAGACG               CGTCTC(1/5)
BsoBI         358  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     1004  TGTACA               T^GTACA
BspEI        1730  TCCGGA               T^CCGGA
BspMI        1293  ACCTGC               ACCTGC(4/8)
BsrGI        1004  TGTACA               T^GTACA
BssSI         612  CACGAG               CACGAG(-5/-1)
EcoRI         437  GAATTC               G^AATTC
EcoT14I       188  CCAAGG               C^C[AT][AT]GG
FbaI          104  TGATCA               T^GATCA
HgaI          378  GACGC                GACGC(5/10)
HincII       1135  GTCAAC               GT[CT]^[GA]AC
HindII       1135  GTCAAC               GT[CT]^[GA]AC
MroI         1730  TCCGGA               T^CCGGA
PmaCI         100  CACGTG               CAC^GTG
PmlI          100  CACGTG               CAC^GTG
ScaI          890  AGTACT               AGT^ACT
SfaNI        1517  GCATC                GCATC(5/9)
SspBI        1004  TGTACA               T^GTACA
StyI          188  CCAAGG               C^C[AT][AT]GG
XmnI          297  GAACTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcsI          437  GAATTC               [GA]^AATT[CT]
              979  AAATTT              
AflIII        851  ACGTGT               A^C[GA][CT]GT
             1560  ACACGT              
Alw44I        181  GTGCAC               G^TGCAC
              250  GTGCAC              
AlwNI        1077  CAGTAACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1710  CAGCTGCTG           
ApaLI         181  GTGCAC               G^TGCAC
              250  GTGCAC              
ApoI          437  GAATTC               [GA]^AATT[CT]
              979  AAATTT              
AvaII         420  GGACC                G^G[AT]CC
             1548  GGTCC               
BbsI          477  GAAGAC               GAAGAC(2/6)
              934  GTCTTC              
BpuAI         477  GAAGAC               GAAGAC(2/6)
              934  GTCTTC              
BsrFI           3  GCCGGC               [GA]^CCGG[CT]
             1628  GCCGGC              
CfoI         1603  GCGC                 GCG^C
             1668  GCGC                
Cfr10I          3  GCCGGC               [GA]^CCGG[CT]
             1628  GCCGGC              
DraII         524  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             1510  GGGGCCT             
EaeI          597  CGGCCA               [CT]^GGCC[GA]
             1037  TGGCCG              
EarI          520  GAAGAG               CTCTTC(1/4)
             1746  GAAGAG              
EcoO109I      524  AGGCCCC              [GA]G^G[ACGT]CC[CT]
             1510  GGGGCCT             
FauI          371  CCCGC                CCCGC(4/6)
              423  CCCGC               
HaeII        1602  GGCGCT               [GA]GCGC^[CT]
             1667  GGCGCT              
HhaI         1603  GCGC                 GCG^C
             1668  GCGC                
HinP1I       1603  GCGC                 G^CGC
             1668  GCGC                
Ksp632I       520  GAAGAG               CTCTTC(1/4)
             1746  GAAGAG              
MseI           86  TTAA                 T^TAA
             1192  TTAA                
NaeI            3  GCCGGC               GCC^GGC
             1628  GCCGGC              
NgoMIV          3  GCCGGC               G^CCGGC
             1628  GCCGGC              
PflMI          83  CCATTAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1680  CCAGGACATGG         
Tru9I          86  TTAA                 T^TAA
             1192  TTAA                
Tsp45I         52  GTCAC                ^GT[GC]AC
              140  GTGAC               
Van91I         83  CCATTAAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1680  CCAGGACATGG         
XcmI          506  CCATCGAGGCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1319  CCAGGCTGCCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AspHI       
BamHI       BanI        BbvI        BcnI        BfaI        
BmyI        BpmI        BsaJI       BsaWI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        TaqI        TfiI        
TseI        Tsp509I     TspRI       TthHB8I     XhoII       
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