Restriction map for pF1KB4614
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BalI        BbeI        
BbrPI       BbvCI       BcgI        BclI        BcnI        
BfrI        BglI        BglII       BlnI        BpmI        
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BsmI        BspDI       
BspEI       BspHI       BspMI       BsrBI       BsrDI       
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraII       DrdI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoNI       EcoO109I    EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NaeI        NarI        NciI        NdeI        
NgoMIV      NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    SspI        StuI        
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AflIII       1646  ACATGT               A^C[GA][CT]GT
AspHI        1225  GAGCAC               G[AT]GC[AT]^C
AvaI          979  CCCGAG               C^[CT]CG[GA]G
AvaII        1285  GGACC                G^G[AT]CC
BanII        1388  GAGCCC               G[GA]GC[CT]^C
BciVI         798  GGATAC               GTATCC(6/5)
BlpI         1222  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1222  GCTGAGC              GC^T[ACGT]AGC
BsiHKAI      1225  GAGCAC               G[AT]GC[AT]^C
BsoBI         979  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      217  TGTACA               T^GTACA
BspLU11I     1646  ACATGT               A^CATGT
BsrFI         273  GCCGGT               [GA]^CCGG[CT]
BsrGI         217  TGTACA               T^GTACA
BstAPI        250  GCATCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1017  GGTTACC              G^GT[ACGT]ACC
BstPI        1017  GGTTACC              G^GT[ACGT]ACC
CelII        1222  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        273  GCCGGT               [GA]^CCGG[CT]
DraIII        724  CACATTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1093  CGGCCA               [CT]^GGCC[GA]
EcoO65I      1017  GGTTACC              G^GT[ACGT]ACC
EcoRI        1327  GAATTC               G^AATTC
MspA1I       1037  CAGCTG               C[AC]G^C[GT]G
NcoI         1614  CCATGG               C^CATGG
NheI          657  GCTAGC               G^CTAGC
PshAI         808  GACTGGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII        1037  CAGCTG               CAG^CTG
SexAI        1271  ACCAGGT              A^CC[AT]GGT
SspBI         217  TGTACA               T^GTACA
TaqI         1440  TCGA                 T^CGA
TthHB8I      1440  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcyI          812  GGCGTC               G[GA]^CG[CT]C
             1066  GGCGTC              
BamHI         679  GGATCC               G^GATCC
             1057  GGATCC              
BbsI          805  GAAGAC               GAAGAC(2/6)
              405  GTCTTC              
BfaI           92  CTAG                 C^TAG
              658  CTAG                
BpuAI         805  GAAGAC               GAAGAC(2/6)
              405  GTCTTC              
BsaHI         812  GGCGTC               G[GA]^CG[CT]C
             1066  GGCGTC              
BseRI         509  CTCCTC               GAGGAG(10/8)
             1378  GAGGAG              
BsgI          257  CTGCAC               GTGCAG(16/14)
              575  CTGCAC              
BstXI          31  CCAGCTCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              861  CCAGATCACTGG        
EarI          674  GAAGAG               CTCTTC(1/4)
              783  GAAGAG              
HapII          48  CCGG                 C^CGG
              274  CCGG                
HgaI          813  GCGTC                GACGC(5/10)
             1067  GCGTC               
Hin1I         812  GGCGTC               G[GA]^CG[CT]C
             1066  GGCGTC              
HpaII          48  CCGG                 C^CGG
              274  CCGG                
Ksp632I       674  GAAGAG               CTCTTC(1/4)
              783  GAAGAG              
MaeI           92  CTAG                 C^TAG
              658  CTAG                
MspI           48  CCGG                 C^CGG
              274  CCGG                
PflMI         817  CCAAGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              997  CCAACCTCTGG         
PleI          183  GACTC                GAGTC(4/5)
              237  GACTC               
SphI          537  GCATGC               GCATG^C
              974  GCATGC              
Van91I        817  CCAAGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              997  CCAACCTCTGG         
XcmI          356  CCACAAACAACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1166  CCAACTCAGCTTTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
Aor51HI     ApoI        BanI        BbvI        BmyI        
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DsaI        Eco47III    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeII       HaeIII      HhaI        
HinP1I      HinfI       HphI        ItaI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XhoII       
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