Restriction map for pF1KB4614
 (Circular) (Six-base) MAPSORT of: pF1KB4614.seq  Check: 3901  from: 1  to: 4901



pF1KB4614  4901 bp

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:25  ..

AccI GT'mk_AC

Cuts at:   1939    1939 
   Size:       4901
 
Acc65I G'GTAC_C

Cuts at:   1917    1917 
   Size:       4901
 
AclI AA'CG_TT

Cuts at:   4741    4741 
   Size:       4901
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    932    1022    2726    3158    3905    4173     932 
   Size:         90    1704     432     747     268    1660

  Fragments arranged by size: 

                1704    1660     747     432     268      90
 
AfeI AGC'GCT

Cuts at:    693     844    1245     693 
   Size:        151     401    4349

  Fragments arranged by size: 

                4349     401     151
 
AflIII A'CryG_T

Cuts at:   1754    2253    3357    4334    1754 
   Size:        499    1104     977    2321

  Fragments arranged by size: 

                2321    1104     977     499
 
AgeI A'CCGG_T

Cuts at:   3289    3289 
   Size:       4901
 
AleI CACnn'nnGTG

Cuts at:    739    1318     739 
   Size:        579    4322
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    668     668 
   Size:       4901
 
AlwNI CAG_nnn'CTG

Cuts at:   2206    3773    2206 
   Size:       1567    3334
 
ApaLI G'TGCA_C

Cuts at:   3671    3671 
   Size:       4901
 
ApoI r'AATT_y

Cuts at:   1435    1525    1732    1890    1905    4254    4510    1435 
   Size:         90     207     158      15    2349     256    1826

  Fragments arranged by size: 

                2349    1826     256     207     158      90      15
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4901
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4901
 
AvaI C'yCGr_G

Cuts at:   1087    1921    1087 
   Size:        834    4067
 
BamHI G'GATC_C

Cuts at:    787    1165    1926     787 
   Size:        378     761    3762

  Fragments arranged by size: 

                3762     761     378
 
BanI G'GyrC_C

Cuts at:    489    1266    1424    1917    2331    2579    2614     489 
   Size:        777     158     493     414     248      35    2776

  Fragments arranged by size: 

                2776     777     493     414     248     158      35
 
BanII G_rGCy'C

Cuts at:   1500    1915    2945    1500 
   Size:        415    1030    3456

  Fragments arranged by size: 

                3456    1030     415
 
BbeI G_GCGC'C

Cuts at:   2335    2583    2335 
   Size:        248    4653
 
BbsI GAAGACnn'nnnn_

Cuts at:    506     920     506 
   Size:        414    4487
 
BciVI GTATCCnnnnn_n'

Cuts at:    900    2799    3560    4901     900 
   Size:       1899     761    1341     900

  Fragments arranged by size: 

                1899    1341     900     761
 
BclI T'GATC_A

Cuts at:   2421    2421 
   Size:       4901
 
BglII A'GATC_T

Cuts at:   2416    4453    2416 
   Size:       2037    2864
 
BlpI GC'TnA_GC

Cuts at:   1331    2013    1331 
   Size:        682    4219
 
Bme1580I G_kGCm'C

Cuts at:    431    2526    2619    3675     431 
   Size:       2095      93    1056    1657

  Fragments arranged by size: 

                2095    1657    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    986    1420    2113    2194    2529    4673     986 
   Size:        434     693      81     335    2144    1214

  Fragments arranged by size: 

                2144    1214     693     434     335      81
 
BmtI G_CTAG'C

Cuts at:    769     769 
   Size:       4901
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3268    3268 
   Size:       4901
 
Bpu10I CC'TnA_GC

Cuts at:    377     377 
   Size:       4901
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2077    2956    3448    3746    2077 
   Size:        879     492     298    3232

  Fragments arranged by size: 

                3232     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4605    4605 
   Size:       4901
 
BsaAI yAC'GTr

Cuts at:   2254    2254 
   Size:       4901
 
BsaBI GATnn'nnATC

Cuts at:      6    2415    4452       6 
   Size:       2409    2037     455

  Fragments arranged by size: 

                2409    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    921    1175    2332    2580    3282    4778     921 
   Size:        254    1157     248     702    1496    1044

  Fragments arranged by size: 

                1496    1157    1044     702     254     248
 
BsaWI w'CCGG_w

Cuts at:   2090    2611    3289    3563    3710    4121    2090 
   Size:        521     678     274     147     411    2870

  Fragments arranged by size: 

                2870     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    608    1501     608 
   Size:        893    4008
 
BseYI C'CCAG_C

Cuts at:    138     561    1295    2326    3084    3661    4157     138 
   Size:        423     734    1031     758     577     496     882

  Fragments arranged by size: 

                1031     882     758     734     577     496     423
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    350     668     350 
   Size:        318    4583
 
BsiEI CG_ry'CG

Cuts at:    104    2443    2489    3697     104 
   Size:       2339      46    1208    1308

  Fragments arranged by size: 

                2339    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1337    1915    2693    2883    3675    1337 
   Size:        578     778     190     792    2563

  Fragments arranged by size: 

                2563     792     778     578     190
 
Bsp1286I G_dGCh'C

Cuts at:    431    1337    1500    1915    2526    2619    2693    2883    2945 
   Size:        906     163     415     611      93      74     190      62

Cuts at:   2945    3675     431 
   Size:        730    1657

  Fragments arranged by size: 

                1657     906     730     611     415     190     163      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:   4121    4121 
   Size:       4901
 
BspHI T'CATG_A

Cuts at:   4077    4077 
   Size:       4901
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1951    2165    2467    2848    1951 
   Size:        214     302     381    4004

  Fragments arranged by size: 

                4004     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    3193    3247    4731    4896      50 
   Size:       3143      54    1484     165      55

  Fragments arranged by size: 

                3143    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2813    2813 
   Size:       4901
 
BsrFI r'CCGG_y

Cuts at:    381    2899    3080    3289     381 
   Size:       2518     181     209    1993

  Fragments arranged by size: 

                2518    1993     209     181
 
BsrGI T'GTAC_A

Cuts at:    325     325 
   Size:       4901
 
BssSI C'ACGA_G

Cuts at:   3172    3530    3172 
   Size:        358    4543
 
BstAPI GCAn_nnn'nTGC

Cuts at:    364    2278     364 
   Size:       1914    2987
 
BstBI TT'CG_AA

Cuts at:   3262    3262 
   Size:       4901
 
BstEII G'GTnAC_C

Cuts at:   1125    1125 
   Size:       4901
 
BstXI CCAn_nnnn'nTGG

Cuts at:    146     976     146 
   Size:        830    4071
 
BstYI r'GATC_y

Cuts at:      1     787    1165    1562    1926    2393    2416    2751    2997 
   Size:        786     378     397     364     467      23     335     246

Cuts at:   2997    4009    4095    4107    4453       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     786     467     449     397     378     364     346
                 335     246      86      23      12
 
BtgI C'CryG_G

Cuts at:    493     602     718    1722     493 
   Size:        109     116    1004    3672

  Fragments arranged by size: 

                3672    1004     116     109
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    263    2851    3032    3195     263 
   Size:       2588     181     163    1969

  Fragments arranged by size: 

                2588    1969     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1449    2144    1449 
   Size:        695    4206
 
DraI TTT'AAA

Cuts at:   1900    1900 
   Size:       4901
 
DraIII CAC_nnn'GTG

Cuts at:    837     837 
   Size:       4901
 
DrdI GACnn_nn'nnGTC

Cuts at:   2607    3465    2607 
   Size:        858    4043
 
EaeI y'GGCC_r

Cuts at:   1201    2486    2660    3051    3078    4423    1201 
   Size:       1285     174     391      27    1345    1679

  Fragments arranged by size: 

                1679    1345    1285     391     174      27
 
EagI C'GGCC_G

Cuts at:   2486    2486 
   Size:       4901
 
EarI CTCTTCn'nnn_

Cuts at:    777     886    2924    3134     777 
   Size:        109    2038     210    2544

  Fragments arranged by size: 

                2544    2038     210     109
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3109    3419    3565    4711    3109 
   Size:        310     146    1146    3299

  Fragments arranged by size: 

                3299    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1913    1913 
   Size:       4901
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    932    1022    2726    3158    3905    4173     932 
   Size:         90    1704     432     747     268    1660

  Fragments arranged by size: 

                1704    1660     747     432     268      90
 
EcoO109I rG'GnC_Cy

Cuts at:   2040    2040 
   Size:       4901
 
EcoRI G'AATT_C

Cuts at:   1435    1905    1435 
   Size:        470    4431
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2920    2920 
   Size:       4901
 
FspI TGC'GCA

Cuts at:   2682    2682 
   Size:       4901
 
HaeII r_GCGC'y

Cuts at:    695     846    1247    2335    2583    3605    4584     695 
   Size:        151     401    1088     248    1022     979    1012

  Fragments arranged by size: 

                1088    1022    1012     979     401     248     151
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1562    1594    2743    2773    2775    2805    1562 
   Size:         32    1149      30       2      30    3658

  Fragments arranged by size: 

                3658    1149      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1940    1940 
   Size:       4901
 
KasI G'GCGC_C

Cuts at:   2331    2579    2331 
   Size:        248    4653
 
KpnI G_GTAC'C

Cuts at:   1921    1921 
   Size:       4901
 
MluI A'CGCG_T

Cuts at:   4334    4334 
   Size:       4901
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3572    3756    3572 
   Size:        184    4717
 
MscI TGG'CCA

Cuts at:   2662    2662 
   Size:       4901
 
MslI CAynn'nnrTG

Cuts at:    650     739     878    1318    1727    2890    3017     650 
   Size:         89     139     440     409    1163     127    2534

  Fragments arranged by size: 

                2534    1163     440     409     139     127      89
 
MspA1I CmG'CkG

Cuts at:   1147    2012    2326    2686    3699    3944    1147 
   Size:        865     314     360    1013     245    2104

  Fragments arranged by size: 

                2104    1013     865     360     314     245
 
NaeI GCC'GGC

Cuts at:   3082    3082 
   Size:       4901
 
NarI GG'CG_CC

Cuts at:   2332    2580    2332 
   Size:        248    4653
 
NcoI C'CATG_G

Cuts at:   1722    1722 
   Size:       4901
 
NgoMIV G'CCGG_C

Cuts at:   3080    3080 
   Size:       4901
 
NheI G'CTAG_C

Cuts at:    765     765 
   Size:       4901
 
NspI r_CATG'y

Cuts at:    186     589     649    1086    1606    1758    1879    1954    2454 
   Size:        403      60     437     520     152     121      75     500

Cuts at:   2454    3361     186 
   Size:        907    1726

  Fragments arranged by size: 

                1726     907     520     500     437     403     152     121
                  75      60
 
PasI CC'CwG_GG

Cuts at:    792     792 
   Size:       4901
 
PciI A'CATG_T

Cuts at:   1754    3357    1754 
   Size:       1603    3298
 
PflMI CCAn_nnn'nTGG

Cuts at:    931    1111     931 
   Size:        180    4721
 
PfoI T'CCnGG_A

Cuts at:   1760    1760 
   Size:       4901
 
PmeI GTTT'AAAC

Cuts at:   1900    1900 
   Size:       4901
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4040    4072    4040 
   Size:         32    4869
 
PshAI GACnn'nnGTC

Cuts at:    920     920 
   Size:       4901
 
PstI C_TGCA'G

Cuts at:    164     708    1146    1948    2633     164 
   Size:        544     438     802     685    2432

  Fragments arranged by size: 

                2432     802     685     544     438
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4901
 
PvuII CAG'CTG

Cuts at:   1147    2326    2686    1147 
   Size:       1179     360    3362

  Fragments arranged by size: 

                3362    1179     360
 
RsrII CG'GwC_CG

Cuts at:   3096    3096 
   Size:       4901
 
SacI G_AGCT'C

Cuts at:   1915    1915 
   Size:       4901
 
SalI G'TCGA_C

Cuts at:   1938    1938 
   Size:       4901
 
SapI GCTCTTCn'nnn_

Cuts at:   2924    3134    2924 
   Size:        210    4691
 
SbfI CC_TGCA'GG

Cuts at:   1948    1948 
   Size:       4901
 
ScaI AGT'ACT

Cuts at:   4418    4418 
   Size:       4901
 
SexAI A'CCwGG_T

Cuts at:   1379    1379 
   Size:       4901
 
SfcI C'TryA_G

Cuts at:     33     117     160     704    1142    1160    1944    2629    3622 
   Size:         84      43     544     438      18     784     685     993

Cuts at:   3622    3813      33 
   Size:        191    1121

  Fragments arranged by size: 

                1121     993     784     685     544     438     191      84
                  43      18
 
SfoI GGC'GCC

Cuts at:   2333    2581    2333 
   Size:        248    4653
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4901
 
SmaI CCC'GGG

Cuts at:   1923    1923 
   Size:       4901
 
SmlI C'TyrA_G

Cuts at:   2056    2971    3463    3725    2056 
   Size:        915     492     262    3232

  Fragments arranged by size: 

                3232     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4901
 
SphI G_CATG'C

Cuts at:    649    1086    1954    2454     649 
   Size:        437     868     500    3096

  Fragments arranged by size: 

                3096     868     500     437
 
StyI C'CwwG_G

Cuts at:    145     925    1209    1566    1722    2035    2389     145 
   Size:        780     284     357     156     313     354    2657

  Fragments arranged by size: 

                2657     780     357     354     313     284     156
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    384    1442    2484    2617    3076    3285     384 
   Size:       1058    1042     133     459     209    2000

  Fragments arranged by size: 

                2000    1058    1042     459     209     133
 
TatI w'GTAC_w

Cuts at:    325    4416     325 
   Size:       4091     810
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4418    4450    4418 
   Size:         32    4869
 
Tth111I GACn'n_nGTC

Cuts at:   2698    2698 
   Size:       4901
 
XbaI T'CTAG_A

Cuts at:   1932    1932 
   Size:       4901
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    471    1281    2396     471 
   Size:        810    1115    2976

  Fragments arranged by size: 

                2976    1115     810
 
XmaI C'CCGG_G

Cuts at:   1921    1921 
   Size:       4901
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AleI
     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NspI     PasI     PciI
    PflMI     PfoI     PmeI     PpiI    PshAI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AloI     ApaI     AscI    AvrII
     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI     BpmI    BsaXI
    BsiWI     BsmI    BsmBI   BssHII  BstZ17I   Bsu36I     ClaI    CspCI
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI
     NotI     NruI     NsiI     PacI     PmlI    PpuMI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI    SnaBI     SrfI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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