Restriction map for pF1KB6611
 (Linear) (Six-base) MAPSORT of: KIBB6611.seq  Check: 9868  from: 1  to: 1236



KIBB6611.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     981    1236 
   Size:        981     255
 
AflIII A'CryG_T

Cuts at:      0     917    1236 
   Size:        917     319
 
AgeI A'CCGG_T

Cuts at:      0     305    1236 
   Size:        305     931
 
AleI CACnn'nnGTG

Cuts at:      0     974    1236 
   Size:        974     262
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     623    1236 
   Size:        623     613
 
AlwNI CAG_nnn'CTG

Cuts at:      0      63    1236 
   Size:         63    1173
 
ApoI r'AATT_y

Cuts at:      0       9    1236 
   Size:          9    1227
 
AvaI C'yCGr_G

Cuts at:      0     585    1236 
   Size:        585     651
 
BanI G'GyrC_C

Cuts at:      0     299     323     564     880    1236 
   Size:        299      24     241     316     356

  Fragments arranged by size: 

                 356     316     299     241      24
 
BanII G_rGCy'C

Cuts at:      0     404    1082    1163    1236 
   Size:        404     678      81      73

  Fragments arranged by size: 

                 678     404      81      73
 
BbeI G_GCGC'C

Cuts at:      0     884    1236 
   Size:        884     352
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      32     996    1236 
   Size:         32     964     240

  Fragments arranged by size: 

                 964     240      32
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     262     730    1236 
   Size:        262     468     506

  Fragments arranged by size: 

                 506     468     262
 
BglI GCCn_nnn'nGGC

Cuts at:      0     683    1236 
   Size:        683     553
 
BglII A'GATC_T

Cuts at:      0     863    1236 
   Size:        863     373
 
Bme1580I G_kGCm'C

Cuts at:      0     567    1236 
   Size:        567     669
 
BmgBI CAC'GTC

Cuts at:      0     918    1236 
   Size:        918     318
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     684    1236 
   Size:        684     552
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     593     802    1236 
   Size:        593     209     434

  Fragments arranged by size: 

                 593     434     209
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     223     241    1236 
   Size:        223      18     995

  Fragments arranged by size: 

                 995     223      18
 
BsaAI yAC'GTr

Cuts at:      0     819     909    1236 
   Size:        819      90     327

  Fragments arranged by size: 

                 819     327      90
 
BsaHI Gr'CG_yC

Cuts at:      0     592     770     881    1236 
   Size:        592     178     111     355

  Fragments arranged by size: 

                 592     355     178     111
 
BsaWI w'CCGG_w

Cuts at:      0     305    1236 
   Size:        305     931
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     904     934    1236 
   Size:        904      30     302

  Fragments arranged by size: 

                 904     302      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     856    1090    1236 
   Size:        856     234     146

  Fragments arranged by size: 

                 856     234     146
 
BseYI C'CCAG_C

Cuts at:      0     389     764    1156    1236 
   Size:        389     375     392      80

  Fragments arranged by size: 

                 392     389     375      80
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376     583    1236 
   Size:        376     207     653

  Fragments arranged by size: 

                 653     376     207
 
BsiEI CG_ry'CG

Cuts at:      0     873    1236 
   Size:        873     363
 
BsiHKAI G_wGCw'C

Cuts at:      0     217     404    1082    1129    1236 
   Size:        217     187     678      47     107

  Fragments arranged by size: 

                 678     217     187     107      47
 
BsmI GAATG_Cn'

Cuts at:      0    1201    1236 
   Size:       1201      35
 
Bsp1286I G_dGCh'C

Cuts at:      0     217     404     567    1082    1129    1163    1236 
   Size:        217     187     163     515      47      34      73

  Fragments arranged by size: 

                 515     217     187     163      73      47      34
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     981     995    1236 
   Size:        981      14     241

  Fragments arranged by size: 

                 981     241      14
 
BsrBI CCG'CTC

Cuts at:      0     852    1026    1236 
   Size:        852     174     210

  Fragments arranged by size: 

                 852     210     174
 
BsrFI r'CCGG_y

Cuts at:      0     305    1236 
   Size:        305     931
 
BsrGI T'GTAC_A

Cuts at:      0     482     920     995    1236 
   Size:        482     438      75     241

  Fragments arranged by size: 

                 482     438     241      75
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     351    1236 
   Size:        351     885
 
BstYI r'GATC_y

Cuts at:      0     158     863    1236 
   Size:        158     705     373

  Fragments arranged by size: 

                 705     373     158
 
BtgI C'CryG_G

Cuts at:      0     226     469     926    1112    1236 
   Size:        226     243     457     186     124

  Fragments arranged by size: 

                 457     243     226     186     124
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1023    1236 
   Size:       1023     213
 
BtsI GCAGTG_nn'

Cuts at:      0     595    1236 
   Size:        595     641
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     686     721    1236 
   Size:        686      35     515

  Fragments arranged by size: 

                 686     515      35
 
DraI TTT'AAA

Cuts at:      0      14    1236 
   Size:         14    1222
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     130    1236 
   Size:        130    1106
 
EaeI y'GGCC_r

Cuts at:      0      74     341    1236 
   Size:         74     267     895

  Fragments arranged by size: 

                 895     267      74
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    1236 
   Size:         19    1217
 
EcoICRI GAG'CTC

Cuts at:      0     402    1080    1236 
   Size:        402     678     156

  Fragments arranged by size: 

                 678     402     156
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     593     802    1236 
   Size:        593     209     434

  Fragments arranged by size: 

                 593     434     209
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     523    1236 
   Size:        523     713
 
EcoO109I rG'GnC_Cy

Cuts at:      0      60    1236 
   Size:         60    1176
 
EcoRV GAT'ATC

Cuts at:      0     199    1236 
   Size:        199    1037
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     819    1236 
   Size:        819     417
 
HaeII r_GCGC'y

Cuts at:      0     382     884    1236 
   Size:        382     502     352

  Fragments arranged by size: 

                 502     382     352
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     716     748    1236 
   Size:        716      32     488

  Fragments arranged by size: 

                 716     488      32
 
HincII GTy'rAC

Cuts at:      0     966    1236 
   Size:        966     270
 
HindIII A'AGCT_T

Cuts at:      0     336    1236 
   Size:        336     900
 
KasI G'GCGC_C

Cuts at:      0     880    1236 
   Size:        880     356
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1166    1236 
   Size:       1166      70
 
MscI TGG'CCA

Cuts at:      0      76     343    1236 
   Size:         76     267     893

  Fragments arranged by size: 

                 893     267      76
 
MslI CAynn'nnrTG

Cuts at:      0     974    1102    1132    1236 
   Size:        974     128      30     104

  Fragments arranged by size: 

                 974     128     104      30
 
MspA1I CmG'CkG

Cuts at:      0      54     471     674     680    1236 
   Size:         54     417     203       6     556

  Fragments arranged by size: 

                 556     417     203      54       6
 
NarI GG'CG_CC

Cuts at:      0     881    1236 
   Size:        881     355
 
NspI r_CATG'y

Cuts at:      0    1101    1236 
   Size:       1101     135
 
PpuMI rG'GwC_Cy

Cuts at:      0      60    1236 
   Size:         60    1176
 
PstI C_TGCA'G

Cuts at:      0      25      59     992    1236 
   Size:         25      34     933     244

  Fragments arranged by size: 

                 933     244      34      25
 
PvuII CAG'CTG

Cuts at:      0      54    1236 
   Size:         54    1182
 
SacI G_AGCT'C

Cuts at:      0     404    1082    1236 
   Size:        404     678     154

  Fragments arranged by size: 

                 678     404     154
 
SacII CC_GC'GG

Cuts at:      0     472    1236 
   Size:        472     764
 
SbfI CC_TGCA'GG

Cuts at:      0     992    1236 
   Size:        992     244
 
SexAI A'CCwGG_T

Cuts at:      0     179     350     524    1236 
   Size:        179     171     174     712

  Fragments arranged by size: 

                 712     179     174     171
 
SfcI C'TryA_G

Cuts at:      0      21      55     437     988    1236 
   Size:         21      34     382     551     248

  Fragments arranged by size: 

                 551     382     248      34      21
 
SfoI GGC'GCC

Cuts at:      0     882    1236 
   Size:        882     354
 
SmaI CCC'GGG

Cuts at:      0     587    1236 
   Size:        587     649
 
SmlI C'TyrA_G

Cuts at:      0     238     256    1236 
   Size:        238      18     980

  Fragments arranged by size: 

                 980     238      18
 
SspI AAT'ATT

Cuts at:      0     175    1236 
   Size:        175    1061
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     582    1236 
   Size:        582     654
 
TatI w'GTAC_w

Cuts at:      0     482     920     995    1236 
   Size:        482     438      75     241

  Fragments arranged by size: 

                 482     438     241      75
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     544     576    1236 
   Size:        544      32     660

  Fragments arranged by size: 

                 660     544      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1212    1236 
   Size:       1212      24
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1149    1236 
   Size:       1149      87
 
XmaI C'CCGG_G

Cuts at:      0     585    1236 
   Size:        585     651
 
XmnI GAAnn'nnTTC

Cuts at:      0     802    1236 
   Size:        802     434
 

 Enzymes that do cut:

     AarI   AflIII     AgeI     AleI     AlfI    AlwNI     ApoI     AvaI
     BanI    BanII     BbeI     BbsI    BciVI     BglI    BglII Bme1580I
    BmgBI     BplI     BpmI    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI
    BsrBI    BsrFI    BsrGI    BstXI    BstYI     BtgI    BtgZI     BtsI
    CspCI     DraI     DrdI     EaeI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII     KasI
     MmeI     MscI     MslI   MspA1I     NarI     NspI    PpuMI     PstI
    PvuII     SacI    SacII     SbfI    SexAI     SfcI     SfoI     SmaI
     SmlI     SspI    TaqII     TatI     TstI  Tth111I     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AhdI
     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI    BbvCI     BcgI     BclI    BfrBI     BlpI     BmrI     BmtI
   Bpu10I     BsaI    BsaBI    BsiWI    BsmBI    BspEI    BspHI    BsrDI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     ClaI
   DraIII     EagI     EarI    EcoRI     FseI     FspI    FspAI     HpaI
     KpnI     MfeI     MluI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SalI    SanDI     SapI     ScaI     SfiI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
     XbaI     XhoI     ZraI
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