Restriction map for pF1KB6580
 (Circular) (Six-base) MAPSORT of: pF1KB6580.seq  Check: 3809  from: 1  to: 4322



pF1KB6580  4322 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:    242    1360     242 
   Size:       1118    3204
 
Acc65I G'GTAC_C

Cuts at:   1338    1338 
   Size:       4322
 
AclI AA'CG_TT

Cuts at:   4162    4162 
   Size:       4322
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2147    2579    3326    3594    2147 
   Size:        432     747     268    2875

  Fragments arranged by size: 

                2875     747     432     268
 
AflIII A'CryG_T

Cuts at:   1674    2778    3755    1674 
   Size:       1104     977    2241

  Fragments arranged by size: 

                2241    1104     977
 
AgeI A'CCGG_T

Cuts at:   2710    2710 
   Size:       4322
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    726     758     802     834     726 
   Size:         32      44      32    4214

  Fragments arranged by size: 

                4214      44      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:   1627    3194    1627 
   Size:       1567    2755
 
ApaLI G'TGCA_C

Cuts at:   3092    3092 
   Size:       4322
 
ApoI r'AATT_y

Cuts at:    346     538    1125    1326    3675    3931     346 
   Size:        192     587     201    2349     256     737

  Fragments arranged by size: 

                2349     737     587     256     201     192
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4322
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4322
 
AvaI C'yCGr_G

Cuts at:   1342    1342 
   Size:       4322
 
BamHI G'GATC_C

Cuts at:   1347    1347 
   Size:       4322
 
BanI G'GyrC_C

Cuts at:   1338    1752    2000    2035    1338 
   Size:        414     248      35    3625

  Fragments arranged by size: 

                3625     414     248      35
 
BanII G_rGCy'C

Cuts at:   1336    2366    1336 
   Size:       1030    3292
 
BbeI G_GCGC'C

Cuts at:   1756    2004    1756 
   Size:        248    4074
 
BbsI GAAGACnn'nnnn_

Cuts at:    386     818     386 
   Size:        432    3890
 
BbvCI CC'TCA_GC

Cuts at:    839     839 
   Size:       4322
 
BciVI GTATCCnnnnn_n'

Cuts at:   2220    2981    4322    2220 
   Size:        761    1341    2220

  Fragments arranged by size: 

                2220    1341     761
 
BclI T'GATC_A

Cuts at:    996    1230    1842     996 
   Size:        234     612    3476

  Fragments arranged by size: 

                3476     612     234
 
BglII A'GATC_T

Cuts at:    426    1837    3874     426 
   Size:       1411    2037     874

  Fragments arranged by size: 

                2037    1411     874
 
BlpI GC'TnA_GC

Cuts at:   1434    1434 
   Size:       4322
 
Bme1580I G_kGCm'C

Cuts at:   1947    2040    3096    1947 
   Size:         93    1056    3173

  Fragments arranged by size: 

                3173    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    608    1534    1615    1950    4094     608 
   Size:        926      81     335    2144     836

  Fragments arranged by size: 

                2144     926     836     335      81
 
BmtI G_CTAG'C

Cuts at:   1187    1187 
   Size:       4322
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    981    2689     981 
   Size:       1708    2614
 
Bpu10I CC'TnA_GC

Cuts at:    839     839 
   Size:       4322
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1498    2377    2869    3167    1498 
   Size:        879     492     298    2653

  Fragments arranged by size: 

                2653     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4026    4026 
   Size:       4322
 
BsaAI yAC'GTr

Cuts at:   1675    1675 
   Size:       4322
 
BsaBI GATnn'nnATC

Cuts at:      6     455    1289    1836    3873       6 
   Size:        449     834     547    2037     455

  Fragments arranged by size: 

                2037     834     547     455     449
 
BsaHI Gr'CG_yC

Cuts at:   1753    2001    2703    4199    1753 
   Size:        248     702    1496    1876

  Fragments arranged by size: 

                1876    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1511    2032    2710    2984    3131    3542    1511 
   Size:        521     678     274     147     411    2291

  Fragments arranged by size: 

                2291     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1177    1177 
   Size:       4322
 
BseYI C'CCAG_C

Cuts at:   1747    2505    3082    3578    1747 
   Size:        758     577     496    2491

  Fragments arranged by size: 

                2491     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1052    1052 
   Size:       4322
 
BsiEI CG_ry'CG

Cuts at:    104    1864    1910    3118     104 
   Size:       1760      46    1208    1308

  Fragments arranged by size: 

                1760    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1336    2114    2304    3096    1336 
   Size:        778     190     792    2562

  Fragments arranged by size: 

                2562     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:   1336    1947    2040    2114    2304    2366    3096    1336 
   Size:        611      93      74     190      62     730    2562

  Fragments arranged by size: 

                2562     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3542    3542 
   Size:       4322
 
BspHI T'CATG_A

Cuts at:   3498    3498 
   Size:       4322
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1372    1586    1888    2269    1372 
   Size:        214     302     381    3425

  Fragments arranged by size: 

                3425     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2614    2668    4152    4317      50 
   Size:       2564      54    1484     165      55

  Fragments arranged by size: 

                2564    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2234    2234 
   Size:       4322
 
BsrFI r'CCGG_y

Cuts at:   2320    2501    2710    2320 
   Size:        181     209    3932

  Fragments arranged by size: 

                3932     209     181
 
BssSI C'ACGA_G

Cuts at:   2593    2951    2593 
   Size:        358    3964
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1699    1699 
   Size:       4322
 
BstBI TT'CG_AA

Cuts at:   2683    2683 
   Size:       4322
 
BstYI r'GATC_y

Cuts at:      1     426    1347    1814    1837    2172    2418    3430    3516 
   Size:        425     921     467      23     335     246    1012      86

Cuts at:   3516    3528    3874       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     921     467     449     425     346     335     246
                  86      23      12
 
BstZ17I GTA'TAC

Cuts at:    243     243 
   Size:       4322
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2272    2453    2616    2272 
   Size:        181     163    3978

  Fragments arranged by size: 

                3978     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1565    1565 
   Size:       4322
 
ClaI AT'CG_AT

Cuts at:   1007    1007 
   Size:       4322
 
DraI TTT'AAA

Cuts at:    545    1321     545 
   Size:        776    3546
 
DrdI GACnn_nn'nnGTC

Cuts at:   2028    2886    2028 
   Size:        858    3464
 
EaeI y'GGCC_r

Cuts at:   1907    2081    2472    2499    3844    1907 
   Size:        174     391      27    1345    2385

  Fragments arranged by size: 

                2385    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1907    1907 
   Size:       4322
 
EarI CTCTTCn'nnn_

Cuts at:   2345    2555    2345 
   Size:        210    4112
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    746    2530    2840    2986    4132     746 
   Size:       1784     310     146    1146     936

  Fragments arranged by size: 

                1784    1146     936     310     146
 
EcoICRI GAG'CTC

Cuts at:   1334    1334 
   Size:       4322
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   2147    2579    3326    3594    2147 
   Size:        432     747     268    2875

  Fragments arranged by size: 

                2875     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    558     796    1461     558 
   Size:        238     665    3419

  Fragments arranged by size: 

                3419     665     238
 
EcoRI G'AATT_C

Cuts at:   1326    1326 
   Size:       4322
 
EcoRV GAT'ATC

Cuts at:    154     864     154 
   Size:        710    3612
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1161    2341    1161 
   Size:       1180    3142
 
FspI TGC'GCA

Cuts at:   2103    2103 
   Size:       4322
 
HaeII r_GCGC'y

Cuts at:    627    1756    2004    3026    4005     627 
   Size:       1129     248    1022     979     944

  Fragments arranged by size: 

                1129    1022     979     944     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    297     329    1153    1185    2164    2194    2196    2226     297 
   Size:         32     824      32     979      30       2      30    2393

  Fragments arranged by size: 

                2393     979     824      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1361    1361 
   Size:       4322
 
KasI G'GCGC_C

Cuts at:   1752    2000    1752 
   Size:        248    4074
 
KpnI G_GTAC'C

Cuts at:   1342    1342 
   Size:       4322
 
MluI A'CGCG_T

Cuts at:   3755    3755 
   Size:       4322
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2993    3177    2993 
   Size:        184    4138
 
MscI TGG'CCA

Cuts at:   2083    2083 
   Size:       4322
 
MslI CAynn'nnrTG

Cuts at:   2311    2438    2311 
   Size:        127    4195
 
MspA1I CmG'CkG

Cuts at:   1433    1747    2107    3120    3365    1433 
   Size:        314     360    1013     245    2390

  Fragments arranged by size: 

                2390    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2503    2503 
   Size:       4322
 
NarI GG'CG_CC

Cuts at:   1753    2001    1753 
   Size:        248    4074
 
NgoMIV G'CCGG_C

Cuts at:   2501    2501 
   Size:       4322
 
NheI G'CTAG_C

Cuts at:   1183    1183 
   Size:       4322
 
NspI r_CATG'y

Cuts at:   1375    1875    2782    1375 
   Size:        500     907    2915

  Fragments arranged by size: 

                2915     907     500
 
PciI A'CATG_T

Cuts at:   2778    2778 
   Size:       4322
 
PmeI GTTT'AAAC

Cuts at:   1321    1321 
   Size:       4322
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3461    3493    3461 
   Size:         32    4290
 
PpuMI rG'GwC_Cy

Cuts at:    796     796 
   Size:       4322
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    817     849     817 
   Size:         32    4290
 
PstI C_TGCA'G

Cuts at:    225    1369    2054     225 
   Size:       1144     685    2493

  Fragments arranged by size: 

                2493    1144     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4322
 
PvuII CAG'CTG

Cuts at:   1747    2107    1747 
   Size:        360    3962
 
RsrII CG'GwC_CG

Cuts at:   2517    2517 
   Size:       4322
 
SacI G_AGCT'C

Cuts at:   1336    1336 
   Size:       4322
 
SalI G'TCGA_C

Cuts at:   1359    1359 
   Size:       4322
 
SapI GCTCTTCn'nnn_

Cuts at:   2345    2555    2345 
   Size:        210    4112
 
SbfI CC_TGCA'GG

Cuts at:   1369    1369 
   Size:       4322
 
ScaI AGT'ACT

Cuts at:   1058    3839    1058 
   Size:       2781    1541
 
SfcI C'TryA_G

Cuts at:     33     221    1365    2050    3043    3234      33 
   Size:        188    1144     685     993     191    1121

  Fragments arranged by size: 

                1144    1121     993     685     191     188
 
SfoI GGC'GCC

Cuts at:   1754    2002    1754 
   Size:        248    4074
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4322
 
SmaI CCC'GGG

Cuts at:   1344    1344 
   Size:       4322
 
SmlI C'TyrA_G

Cuts at:   1477    2392    2884    3146    1477 
   Size:        915     492     262    2653

  Fragments arranged by size: 

                2653     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4322
 
SphI G_CATG'C

Cuts at:   1375    1875    1375 
   Size:        500    3822
 
SspI AAT'ATT

Cuts at:    255     255 
   Size:       4322
 
StyI C'CwwG_G

Cuts at:   1456    1810    1456 
   Size:        354    3968
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1905    2038    2497    2706    1905 
   Size:        133     459     209    3521

  Fragments arranged by size: 

                3521     459     209     133
 
TatI w'GTAC_w

Cuts at:    824    1056    1142    3837     824 
   Size:        232      86    2695    1309

  Fragments arranged by size: 

                2695    1309     232      86
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3839    3871    3839 
   Size:         32    4290
 
Tth111I GACn'n_nGTC

Cuts at:   2119    2119 
   Size:       4322
 
XbaI T'CTAG_A

Cuts at:   1353    1353 
   Size:       4322
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1817    1817 
   Size:       4322
 
XmaI C'CCGG_G

Cuts at:   1342    1342 
   Size:       4322
 
XmnI GAAnn'nnTTC

Cuts at:    713     713 
   Size:       4322
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AloI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstYI  BstZ17I    BtgZI     BtsI     ClaI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NheI     NspI     PciI     PmeI     PpiI    PpuMI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     ApaI
     AscI    AvrII     BaeI     BcgI    BfrBI     BglI    BmgBI     BpmI
    BsaXI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII    BstXI
   Bsu36I     BtgI    CspCI   DraIII    EcoNI     FseI    FspAI  HindIII
     HpaI     MfeI     NcoI     NdeI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmlI    PshAI     PsiI   PspOMI    PspXI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI
     SwaI     XhoI     ZraI
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