Restriction map for pF1KE2427
 (Linear) (Six-base) MAPSORT of: KIEE2427.seq  Check: 7644  from: 1  to: 1893



KIEE2427.orf

 With 182 enzymes: SgfI * 

                        November 16, 2012 10:46  ..

AccI GT'mk_AC

Cuts at:      0    1775    1893 
   Size:       1775     118
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     705     741    1133    1893 
   Size:        705      36     392     760

  Fragments arranged by size: 

                 760     705     392      36
 
AflIII A'CryG_T

Cuts at:      0    1370    1793    1893 
   Size:       1370     423     100

  Fragments arranged by size: 

                1370     423     100
 
AgeI A'CCGG_T

Cuts at:      0    1067    1893 
   Size:       1067     826
 
AleI CACnn'nnGTG

Cuts at:      0    1618    1893 
   Size:       1618     275
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1873    1893 
   Size:       1873      20
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     724     756    1893 
   Size:        724      32    1137

  Fragments arranged by size: 

                1137     724      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      59     492     778     897    1584    1893 
   Size:         59     433     286     119     687     309

  Fragments arranged by size: 

                 687     433     309     286     119      59
 
ApoI r'AATT_y

Cuts at:      0     138    1002    1018    1411    1574    1893 
   Size:        138     864      16     393     163     319

  Fragments arranged by size: 

                 864     393     319     163     138      16
 
AvaI C'yCGr_G

Cuts at:      0     580    1181    1893 
   Size:        580     601     712

  Fragments arranged by size: 

                 712     601     580
 
BamHI G'GATC_C

Cuts at:      0    1679    1893 
   Size:       1679     214
 
BanI G'GyrC_C

Cuts at:      0       3     192    1429    1690    1893 
   Size:          3     189    1237     261     203

  Fragments arranged by size: 

                1237     261     203     189       3
 
BanII G_rGCy'C

Cuts at:      0      66     832    1094    1893 
   Size:         66     766     262     799

  Fragments arranged by size: 

                 799     766     262      66
 
BbeI G_GCGC'C

Cuts at:      0       7    1893 
   Size:          7    1886
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      18     970    1330    1893 
   Size:         18     952     360     563

  Fragments arranged by size: 

                 952     563     360      18
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1200    1234    1893 
   Size:       1200      34     659

  Fragments arranged by size: 

                1200     659      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1383    1893 
   Size:       1383     510
 
BglI GCCn_nnn'nGGC

Cuts at:      0     283    1870    1893 
   Size:        283    1587      23

  Fragments arranged by size: 

                1587     283      23
 
BglII A'GATC_T

Cuts at:      0     226     302     327     371    1103    1893 
   Size:        226      76      25      44     732     790

  Fragments arranged by size: 

                 790     732     226      76      44      25
 
Bme1580I G_kGCm'C

Cuts at:      0     441    1213    1893 
   Size:        441     772     680

  Fragments arranged by size: 

                 772     680     441
 
BmgBI CAC'GTC

Cuts at:      0      99    1893 
   Size:         99    1794
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     762    1893 
   Size:        762    1131
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580     943    1893 
   Size:        580     363     950

  Fragments arranged by size: 

                 950     580     363
 
Bpu10I CC'TnA_GC

Cuts at:      0     188    1893 
   Size:        188    1705
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     313    1893 
   Size:        313    1580
 
BsaAI yAC'GTr

Cuts at:      0     804    1893 
   Size:        804    1089
 
BsaBI GATnn'nnATC

Cuts at:      0    1154    1893 
   Size:       1154     739
 
BsaHI Gr'CG_yC

Cuts at:      0       4      70    1893 
   Size:          4      66    1823

  Fragments arranged by size: 

                1823      66       4
 
BsaWI w'CCGG_w

Cuts at:      0    1067    1682    1893 
   Size:       1067     615     211

  Fragments arranged by size: 

                1067     615     211
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1701    1731    1893 
   Size:       1701      30     162

  Fragments arranged by size: 

                1701     162      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     478     546     559    1280    1893 
   Size:        478      68      13     721     613

  Fragments arranged by size: 

                 721     613     478      68      13
 
BseYI C'CCAG_C

Cuts at:      0     241     515    1194    1758    1865    1893 
   Size:        241     274     679     564     107      28

  Fragments arranged by size: 

                 679     564     274     241     107      28
 
BsiHKAI G_wGCw'C

Cuts at:      0     189     785    1094    1893 
   Size:        189     596     309     799

  Fragments arranged by size: 

                 799     596     309     189
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     457    1893 
   Size:        457    1436
 
Bsp1286I G_dGCh'C

Cuts at:      0      66     189     441     785     832    1094    1213    1893 
   Size:         66     123     252     344      47     262     119     680

  Fragments arranged by size: 

                 680     344     262     252     123     119      66      47
            
 
BspEI T'CCGG_A

Cuts at:      0    1682    1893 
   Size:       1682     211
 
BspHI T'CATG_A

Cuts at:      0     846    1007    1893 
   Size:        846     161     886

  Fragments arranged by size: 

                 886     846     161
 
BsrDI GCAATG_nn'

Cuts at:      0     252    1163    1893 
   Size:        252     911     730

  Fragments arranged by size: 

                 911     730     252
 
BsrFI r'CCGG_y

Cuts at:      0    1067    1893 
   Size:       1067     826
 
BsrGI T'GTAC_A

Cuts at:      0    1373    1893 
   Size:       1373     520
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     436    1893 
   Size:        436    1457
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     249    1327    1484    1893 
   Size:        249    1078     157     409

  Fragments arranged by size: 

                1078     409     249     157
 
BstYI r'GATC_y

Cuts at:      0     226     302     327     371    1103    1679    1893 
   Size:        226      76      25      44     732     576     214

  Fragments arranged by size: 

                 732     576     226     214      76      44      25
 
Bsu36I CC'TnA_GG

Cuts at:      0     529     824    1517    1893 
   Size:        529     295     693     376

  Fragments arranged by size: 

                 693     529     376     295
 
BtgI C'CryG_G

Cuts at:      0    1145    1616    1893 
   Size:       1145     471     277

  Fragments arranged by size: 

                1145     471     277
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1203    1893 
   Size:       1203     690
 
BtsI GCAGTG_nn'

Cuts at:      0    1634    1836    1893 
   Size:       1634     202      57

  Fragments arranged by size: 

                1634     202      57
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1098    1133    1893 
   Size:       1098      35     760

  Fragments arranged by size: 

                1098     760      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     436    1893 
   Size:        436    1457
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1045    1542    1893 
   Size:       1045     497     351

  Fragments arranged by size: 

                1045     497     351
 
EaeI y'GGCC_r

Cuts at:      0    1329    1817    1893 
   Size:       1329     488      76

  Fragments arranged by size: 

                1329     488      76
 
EarI CTCTTCn'nnn_

Cuts at:      0     265    1597    1672    1893 
   Size:        265    1332      75     221

  Fragments arranged by size: 

                1332     265     221      75
 
EcoICRI GAG'CTC

Cuts at:      0    1092    1893 
   Size:       1092     801
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580     705     741     943    1133    1893 
   Size:        580     125      36     202     190     760

  Fragments arranged by size: 

                 760     580     202     190     125      36
 
EcoO109I rG'GnC_Cy

Cuts at:      0     285     555    1607    1893 
   Size:        285     270    1052     286

  Fragments arranged by size: 

                1052     286     285     270
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     349     750     987    1893 
   Size:        349     401     237     906

  Fragments arranged by size: 

                 906     401     349     237
 
HaeII r_GCGC'y

Cuts at:      0       7    1893 
   Size:          7    1886
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      60      92     287     312     319     343     344     375 
   Size:         60      32     195      25       7      24       1      31

Cuts at:    375     938     970    1088    1120    1408    1440    1893 
   Size:        563      32     118      32     288      32     453

  Fragments arranged by size: 

                 563     453     288     195     118      60      32      32
                  32      32      31      25      24       7       1
 
KasI G'GCGC_C

Cuts at:      0       3    1893 
   Size:          3    1890
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     863    1332    1727    1893 
   Size:        863     469     395     166

  Fragments arranged by size: 

                 863     469     395     166
 
MscI TGG'CCA

Cuts at:      0    1331    1819    1893 
   Size:       1331     488      74

  Fragments arranged by size: 

                1331     488      74
 
MslI CAynn'nnrTG

Cuts at:      0    1618    1893 
   Size:       1618     275
 
MspA1I CmG'CkG

Cuts at:      0     343     705     775    1257    1893 
   Size:        343     362      70     482     636

  Fragments arranged by size: 

                 636     482     362     343      70
 
NarI GG'CG_CC

Cuts at:      0       4    1893 
   Size:          4    1889
 
NspI r_CATG'y

Cuts at:      0     215     928    1750    1797    1893 
   Size:        215     713     822      47      96

  Fragments arranged by size: 

                 822     713     215      96      47
 
PciI A'CATG_T

Cuts at:      0    1793    1893 
   Size:       1793     100
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      59    1338    1893 
   Size:         59    1279     555

  Fragments arranged by size: 

                1279     555      59
 
PmlI CAC'GTG

Cuts at:      0     804    1893 
   Size:        804    1089
 
PpuMI rG'GwC_Cy

Cuts at:      0     555    1893 
   Size:        555    1338
 
PshAI GACnn'nnGTC

Cuts at:      0    1303    1893 
   Size:       1303     590
 
PstI C_TGCA'G

Cuts at:      0     497     774    1893 
   Size:        497     277    1119

  Fragments arranged by size: 

                1119     497     277
 
PvuII CAG'CTG

Cuts at:      0     343     705     775    1257    1893 
   Size:        343     362      70     482     636

  Fragments arranged by size: 

                 636     482     362     343      70
 
SacI G_AGCT'C

Cuts at:      0    1094    1893 
   Size:       1094     799
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1672    1893 
   Size:       1672     221
 
SfcI C'TryA_G

Cuts at:      0     493     770     980    1893 
   Size:        493     277     210     913

  Fragments arranged by size: 

                 913     493     277     210
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     283    1893 
   Size:        283    1610
 
SfoI GGC'GCC

Cuts at:      0       5    1893 
   Size:          5    1888
 
SmaI CCC'GGG

Cuts at:      0    1183    1893 
   Size:       1183     710
 
StyI C'CwwG_G

Cuts at:      0     180     413    1205    1893 
   Size:        180     233     792     688

  Fragments arranged by size: 

                 792     688     233     180
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1185    1893 
   Size:       1185     708
 
TatI w'GTAC_w

Cuts at:      0    1373    1893 
   Size:       1373     520
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1701    1733    1893 
   Size:       1701      32     160

  Fragments arranged by size: 

                1701     160      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      96    1893 
   Size:         96    1797
 
XmaI C'CCGG_G

Cuts at:      0    1181    1893 
   Size:       1181     712
 
XmnI GAAnn'nnTTC

Cuts at:      0    1555    1893 
   Size:       1555     338
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AgeI     AleI     AlfI     AloI    AlwNI
     ApoI     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI
    BciVI     BglI    BglII Bme1580I    BmgBI     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BsrDI    BsrFI    BsrGI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI
   DraIII     DrdI     EaeI     EarI  EcoICRI  Eco57MI EcoO109I     FalI
    HaeII    Hin4I     KasI     MmeI     MscI     MslI   MspA1I     NarI
     NspI     PciI    PflMI     PmlI    PpuMI    PshAI     PstI    PvuII
     SacI     SapI     SfcI     SfiI     SfoI     SmaI     StyI    TaqII
     TatI     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AhdI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI     BclI
    BfrBI     BlpI     BmrI     BmtI    BpuEI     BsgI    BsiEI    BsiWI
     BsmI    BspMI    BsrBI   BssHII    BssSI    BstBI   BstEII  BstZ17I
     ClaI     DraI     EagI     EciI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PfoI     PmeI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI  Tth111I     XbaI     XhoI     ZraI
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