Restriction map for pF1KB6982
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstZ17I     CelII       
ClaI        CpoI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FokI        FseI        FspI        
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MaeII       MamI        MluI        
MluNI       MroI        MscI        MslI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
SspBI       SspI        StyI        SwaI        TaqI        
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AlwNI         116  CAGTAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          445  CCTCAGG              CC^T[ACGT]AGG
AvaI           52  CCCGAG               C^[CT]CG[GA]G
BfaI          724  CTAG                 C^TAG
BmyI          278  GGGCAC               G[AGT]GC[ACT]^C
BsaWI         241  ACCGGA               [AT]^CCGG[AT]
BsgI          140  GTGCAG               GTGCAG(16/14)
BsiYI          53  CCGAGGGTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           53  CCGAGGGTCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI         734  GAGAC                GTCTC(1/5)
BsmFI           3  GTCCC                GGGAC(10/14)
BsmI          381  GAATGC               GAATGC(1/-1)
BsoBI          52  CCCGAG               C^[CT]CG[GA]G
Bsp1286I      278  GGGCAC               G[AGT]GC[ACT]^C
BspMI         469  GCAGGT               ACCTGC(4/8)
BsrFI          19  GCCGGT               [GA]^CCGG[CT]
BsrI          299  ACTGG                ACTGG(1/-1)
BstYI         262  AGATCC               [GA]^GATC[CT]
Bsu36I        445  CCTCAGG              CC^T[ACGT]AGG
Cac8I         280  GCACGC               GC[ACGT]^[ACGT]GC
CfoI           17  GCGC                 GCG^C
Cfr10I         19  GCCGGT               [GA]^CCGG[CT]
Cfr13I        442  GGCCC                G^G[ACGT]CC
DraI          487  TTTAAA               TTT^AAA
DraII         441  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI            6  CCGTGG               C^C[GA][CT]GG
EarI          433  GAAGAG               CTCTTC(1/4)
Eco81I        445  CCTCAGG              CC^T[ACGT]AGG
EcoO109I      441  AGGCCCT              [GA]G^G[ACGT]CC[CT]
FauI          502  GCGGG                CCCGC(4/6)
HaeII          16  AGCGCC               [GA]GCGC^[CT]
HhaI           17  GCGC                 GCG^C
HinP1I         17  GCGC                 G^CGC
HincII        522  GTCAAC               GT[CT]^[GA]AC
HindII        522  GTCAAC               GT[CT]^[GA]AC
Ksp632I       433  GAAGAG               CTCTTC(1/4)
MaeI          724  CTAG                 C^TAG
MfeI          620  CAATTG               C^AATTG
MflI          262  AGATCC               [GA]^GATC[CT]
MunI          620  CAATTG               C^AATTG
NlaIV          10  GGTTCC               GG[ACGT]^[ACGT]CC
PvuII         251  CAGCTG               CAG^CTG
SapI          433  GAAGAGC              GCTCTTC(1/4)
Sau96I        442  GGCCC                G^G[ACGT]CC
SbfI          466  CCTGCAGG             CCTGCA^GG
SfaNI         706  GATGC                GCATC(5/9)
Sse8387I      466  CCTGCAGG             CCTGCA^GG
StuI          394  AGGCCT               AGG^CCT
XhoII         262  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI          370  GTCTAC               GT^[AC][GT]AC
              606  GTCTAC              
AciI           30  CCGC                 CCGC(-3/-1)
              502  GCGG                
AcsI          556  AAATTT               [GA]^AATT[CT]
              676  AAATTC              
AfaI          166  GTAC                 GT^AC
              271  GTAC                
AlwI          263  GATCC                GGATC(4/5)
              463  GATCC               
ApoI          556  AAATTT               [GA]^AATT[CT]
              676  AAATTC              
BbvI          292  GCAGC                GCAGC(8/12)
              499  GCAGC               
BssKI          99  CCAGG                ^CC[ACGT]GG
              267  CCAGG               
BstNI          99  CCAGG                CC^[AT]GG
              267  CCAGG               
DpnII         263  GATC                 ^GATC
              463  GATC                
EcoRII         99  CCAGG                ^CC[AT]GG
              267  CCAGG               
Fnu4HI        292  GCAGC                GC^[ACGT]GC
              499  GCAGC               
HapII          20  CCGG                 C^CGG
              242  CCGG                
HpaII          20  CCGG                 C^CGG
              242  CCGG                
HphI           49  TCACC                GGTGA(8/7)
              711  TCACC               
ItaI          292  GCAGC                GC^[ACGT]GC
              499  GCAGC               
MboI          263  GATC                 ^GATC
              463  GATC                
MspA1I        251  CAGCTG               C[AC]G^C[GT]G
              500  CAGCGG              
MspI           20  CCGG                 C^CGG
              242  CCGG                
MvaI           99  CCAGG                CC^[AT]GG
              267  CCAGG               
MwoI           82  GCTTTTAAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              493  GCCACTGCAGC         
NdeII         263  GATC                 ^GATC
              463  GATC                
NlaIII        336  CATG                 CATG^
              599  CATG                
NspI          335  ACATGC               [GA]CATG^[CT]
              598  ACATGC              
PleI          198  GACTC                GAGTC(4/5)
              520  GAGTC               
PstI          467  CTGCAG               CTGCA^G
              497  CTGCAG              
RsaI          166  GTAC                 GT^AC
              271  GTAC                
Sau3AI        263  GATC                 ^GATC
              463  GATC                
ScrFI          99  CCAGG                CC^[ACGT]GG
              267  CCAGG               
SfcI          467  CTGCAG               C^T[GA][CT]AG
              497  CTGCAG              
TseI          292  GCAGC                G^C[AT]GC
              499  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AluI        BsaJI       DdeI        HaeIII      HinfI       
MaeIII      MboII       MnlI        MseI        TfiI        
Tru9I       Tsp45I      Tsp509I     TspRI       
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