Restriction map for pF1KB8616
 (Circular) (Six-base) MAPSORT of: pF1KB8616.seq  Check: 593  from: 1  to: 4217



pF1KB8616  4217 bp

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AatII G_ACGT'C

Cuts at:    510     510 
   Size:       4217
 
AccI GT'mk_AC

Cuts at:   1255    1255 
   Size:       4217
 
Acc65I G'GTAC_C

Cuts at:    591    1233     591 
   Size:        642    3575
 
AclI AA'CG_TT

Cuts at:   4057    4057 
   Size:       4217
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    521     626    1027    1139    2042    2474    3221    3489     521 
   Size:        105     401     112     903     432     747     268    1249

  Fragments arranged by size: 

                1249     903     747     432     401     268     112     105
            
 
AflIII A'CryG_T

Cuts at:   1569    2673    3650    1569 
   Size:       1104     977    2136

  Fragments arranged by size: 

                2136    1104     977
 
AgeI A'CCGG_T

Cuts at:   2605    2605 
   Size:       4217
 
AhdI GACnn_n'nnGTC

Cuts at:   1194    1194 
   Size:       4217
 
AlwNI CAG_nnn'CTG

Cuts at:   1522    3089    1522 
   Size:       1567    2650
 
ApaI G_GGCC'C

Cuts at:    568     568 
   Size:       4217
 
ApaLI G'TGCA_C

Cuts at:   2987    2987 
   Size:       4217
 
ApoI r'AATT_y

Cuts at:    223     408     850    1207    1221    3570    3826     223 
   Size:        185     442     357      14    2349     256     614

  Fragments arranged by size: 

                2349     614     442     357     256     185      14
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4217
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4217
 
AvaI C'yCGr_G

Cuts at:    145    1237     145 
   Size:       1092    3125
 
BamHI G'GATC_C

Cuts at:   1242    1242 
   Size:       4217
 
BanI G'GyrC_C

Cuts at:    591    1233    1647    1895    1930     591 
   Size:        642     414     248      35    2878

  Fragments arranged by size: 

                2878     642     414     248      35
 
BanII G_rGCy'C

Cuts at:    137     568    1231    2261     137 
   Size:        431     663    1030    2093

  Fragments arranged by size: 

                2093    1030     663     431
 
BbeI G_GCGC'C

Cuts at:   1651    1899    1651 
   Size:        248    3969
 
BbsI GAAGACnn'nnnn_

Cuts at:   1145    1145 
   Size:       4217
 
BciVI GTATCCnnnnn_n'

Cuts at:   2115    2876    4217    2115 
   Size:        761    1341    2115

  Fragments arranged by size: 

                2115    1341     761
 
BclI T'GATC_A

Cuts at:   1737    1737 
   Size:       4217
 
BglII A'GATC_T

Cuts at:    257    1732    3769     257 
   Size:       1475    2037     705

  Fragments arranged by size: 

                2037    1475     705
 
BlpI GC'TnA_GC

Cuts at:   1329    1329 
   Size:       4217
 
Bme1580I G_kGCm'C

Cuts at:    568     839    1842    1935    2991     568 
   Size:        271    1003      93    1056    1794

  Fragments arranged by size: 

                1794    1056    1003     271      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    826    1429    1510    1845    3989     826 
   Size:        603      81     335    2144    1054

  Fragments arranged by size: 

                2144    1054     603     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    932    2584     932 
   Size:       1652    2565
 
Bpu10I CC'TnA_GC

Cuts at:    358     358 
   Size:       4217
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1393    2272    2764    3062    1393 
   Size:        879     492     298    2548

  Fragments arranged by size: 

                2548     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    920    3921     920 
   Size:       3001    1216
 
BsaAI yAC'GTr

Cuts at:   1570    1570 
   Size:       4217
 
BsaBI GATnn'nnATC

Cuts at:      6    1731    3768       6 
   Size:       1725    2037     455

  Fragments arranged by size: 

                2037    1725     455
 
BsaHI Gr'CG_yC

Cuts at:    507    1648    1896    2598    4094     507 
   Size:       1141     248     702    1496     630

  Fragments arranged by size: 

                1496    1141     702     630     248
 
BsaWI w'CCGG_w

Cuts at:   1406    1927    2605    2879    3026    3437    1406 
   Size:        521     678     274     147     411    2186

  Fragments arranged by size: 

                2186     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1084    1084 
   Size:       4217
 
BseYI C'CCAG_C

Cuts at:    567     839    1642    2400    2977    3473     567 
   Size:        272     803     758     577     496    1311

  Fragments arranged by size: 

                1311     803     758     577     496     272
 
BsiEI CG_ry'CG

Cuts at:    104    1759    1805    3013     104 
   Size:       1655      46    1208    1308

  Fragments arranged by size: 

                1655    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    115    1231    2009    2199    2991     115 
   Size:       1116     778     190     792    1341

  Fragments arranged by size: 

                1341    1116     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    115     137     568     839    1231    1842    1935    2009    2199 
   Size:         22     431     271     392     611      93      74     190

Cuts at:   2199    2261    2991     115 
   Size:         62     730    1341

  Fragments arranged by size: 

                1341     730     611     431     392     271     190      93
                  74      62      22
 
BspEI T'CCGG_A

Cuts at:   3437    3437 
   Size:       4217
 
BspHI T'CATG_A

Cuts at:   3393    3393 
   Size:       4217
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1267    1481    1783    2164    1267 
   Size:        214     302     381    3320

  Fragments arranged by size: 

                3320     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     936    2509    2563    4047    4212      50 
   Size:        886    1573      54    1484     165      55

  Fragments arranged by size: 

                1573    1484     886     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    290     707     897    2129     290 
   Size:        417     190    1232    2378

  Fragments arranged by size: 

                2378    1232     417     190
 
BsrFI r'CCGG_y

Cuts at:   2215    2396    2605    2215 
   Size:        181     209    3827

  Fragments arranged by size: 

                3827     209     181
 
BssSI C'ACGA_G

Cuts at:   2488    2846    2488 
   Size:        358    3859
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1594    1594 
   Size:       4217
 
BstBI TT'CG_AA

Cuts at:   2578    2578 
   Size:       4217
 
BstXI CCAn_nnnn'nTGG

Cuts at:    502     910     502 
   Size:        408    3809
 
BstYI r'GATC_y

Cuts at:      1     257    1242    1709    1732    2067    2313    3325    3411 
   Size:        256     985     467      23     335     246    1012      86

Cuts at:   3411    3423    3769       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     985     467     449     346     335     256     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    123     625     123 
   Size:        502    3715
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2167    2348    2511    2167 
   Size:        181     163    3873

  Fragments arranged by size: 

                3873     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1460    1460 
   Size:       4217
 
DraI TTT'AAA

Cuts at:    855    1216     855 
   Size:        361    3856
 
DrdI GACnn_nn'nnGTC

Cuts at:    506    1923    2781     506 
   Size:       1417     858    1942

  Fragments arranged by size: 

                1942    1417     858
 
EaeI y'GGCC_r

Cuts at:   1802    1976    2367    2394    3739    1802 
   Size:        174     391      27    1345    2280

  Fragments arranged by size: 

                2280    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1802    1802 
   Size:       4217
 
EarI CTCTTCn'nnn_

Cuts at:    524     901     926    2240    2450     524 
   Size:        377      25    1314     210    2291

  Fragments arranged by size: 

                2291    1314     377     210      25
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2425    2735    2881    4027    2425 
   Size:        310     146    1146    2615

  Fragments arranged by size: 

                2615    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1229    1229 
   Size:       4217
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    521     626    1027    1139    2042    2474    3221    3489     521 
   Size:        105     401     112     903     432     747     268    1249

  Fragments arranged by size: 

                1249     903     747     432     401     268     112     105
            
 
EcoO109I rG'GnC_Cy

Cuts at:    564    1356     564 
   Size:        792    3425
 
EcoRI G'AATT_C

Cuts at:    408    1207    1221     408 
   Size:        799      14    3404

  Fragments arranged by size: 

                3404     799      14
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2236    2236 
   Size:       4217
 
FspI TGC'GCA

Cuts at:    214    1998     214 
   Size:       1784    2433
 
HaeII r_GCGC'y

Cuts at:    198    1651    1899    2921    3900     198 
   Size:       1453     248    1022     979     515

  Fragments arranged by size: 

                1453    1022     979     515     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    418     450    1122    1154    1180    1183    1212    1215    2059 
   Size:         32     672      32      26       3      29       3     844

Cuts at:   2059    2089    2091    2121     418 
   Size:         30       2      30    2514

  Fragments arranged by size: 

                2514     844     672      32      32      30      30      29
                  26       3       3       2
 
HincII GTy'rAC

Cuts at:   1256    1256 
   Size:       4217
 
KasI G'GCGC_C

Cuts at:   1647    1895    1647 
   Size:        248    3969
 
KpnI G_GTAC'C

Cuts at:    595    1237     595 
   Size:        642    3575
 
MfeI C'AATT_G

Cuts at:    371     371 
   Size:       4217
 
MluI A'CGCG_T

Cuts at:   3650    3650 
   Size:       4217
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2888    3072    2888 
   Size:        184    4033
 
MscI TGG'CCA

Cuts at:   1978    1978 
   Size:       4217
 
MslI CAynn'nnrTG

Cuts at:    805     962    2206    2333     805 
   Size:        157    1244     127    2689

  Fragments arranged by size: 

                2689    1244     157     127
 
MspA1I CmG'CkG

Cuts at:    125     162     627     843    1014    1328    1642    2002    3015 
   Size:         37     465     216     171     314     314     360    1013

Cuts at:   3015    3260     125 
   Size:        245    1082

  Fragments arranged by size: 

                1082    1013     465     360     314     314     245     216
                 171      37
 
NaeI GCC'GGC

Cuts at:   2398    2398 
   Size:       4217
 
NarI GG'CG_CC

Cuts at:   1648    1896    1648 
   Size:        248    3969
 
NdeI CA'TA_TG

Cuts at:   1001    1001 
   Size:       4217
 
NgoMIV G'CCGG_C

Cuts at:   2396    2396 
   Size:       4217
 
NspI r_CATG'y

Cuts at:   1270    1770    2677    1270 
   Size:        500     907    2810

  Fragments arranged by size: 

                2810     907     500
 
PciI A'CATG_T

Cuts at:   2673    2673 
   Size:       4217
 
PfoI T'CCnGG_A

Cuts at:    444     444 
   Size:       4217
 
PmeI GTTT'AAAC

Cuts at:   1216    1216 
   Size:       4217
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3356    3388    3356 
   Size:         32    4185
 
PsiI TTA'TAA

Cuts at:   1136    1136 
   Size:       4217
 
PspOMI G'GGCC_C

Cuts at:    564     564 
   Size:       4217
 
PstI C_TGCA'G

Cuts at:   1264    1949    1264 
   Size:        685    3532
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4217
 
PvuII CAG'CTG

Cuts at:    843    1014    1642    2002     843 
   Size:        171     628     360    3058

  Fragments arranged by size: 

                3058     628     360     171
 
RsrII CG'GwC_CG

Cuts at:   2412    2412 
   Size:       4217
 
SacI G_AGCT'C

Cuts at:   1231    1231 
   Size:       4217
 
SacII CC_GC'GG

Cuts at:    126     628     126 
   Size:        502    3715
 
SalI G'TCGA_C

Cuts at:   1254    1254 
   Size:       4217
 
SapI GCTCTTCn'nnn_

Cuts at:    926    2240    2450     926 
   Size:       1314     210    2693

  Fragments arranged by size: 

                2693    1314     210
 
SbfI CC_TGCA'GG

Cuts at:   1264    1264 
   Size:       4217
 
ScaI AGT'ACT

Cuts at:    750    3734     750 
   Size:       2984    1233
 
SfcI C'TryA_G

Cuts at:     33     458    1260    1945    2938    3129      33 
   Size:        425     802     685     993     191    1121

  Fragments arranged by size: 

                1121     993     802     685     425     191
 
SfoI GGC'GCC

Cuts at:   1649    1897    1649 
   Size:        248    3969
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4217
 
SmaI CCC'GGG

Cuts at:   1239    1239 
   Size:       4217
 
SmlI C'TyrA_G

Cuts at:   1372    2287    2779    3041    1372 
   Size:        915     492     262    2548

  Fragments arranged by size: 

                2548     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67     438      67 
   Size:        371    3846
 
SphI G_CATG'C

Cuts at:   1270    1770    1270 
   Size:        500    3717
 
SspI AAT'ATT

Cuts at:   1119    1119 
   Size:       4217
 
StuI AGG'CCT

Cuts at:    767     767 
   Size:       4217
 
StyI C'CwwG_G

Cuts at:    717    1034    1351    1705     717 
   Size:        317     317     354    3229

  Fragments arranged by size: 

                3229     354     317     317
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1800    1933    2392    2601    1800 
   Size:        133     459     209    3416

  Fragments arranged by size: 

                3416     459     209     133
 
TatI w'GTAC_w

Cuts at:    548     748    3732     548 
   Size:        200    2984    1033

  Fragments arranged by size: 

                2984    1033     200
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3734    3766    3734 
   Size:         32    4185
 
Tth111I GACn'n_nGTC

Cuts at:   2014    2014 
   Size:       4217
 
XbaI T'CTAG_A

Cuts at:   1248    1248 
   Size:       4217
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1712    1712 
   Size:       4217
 
XmaI C'CCGG_G

Cuts at:   1237    1237 
   Size:       4217
 
ZraI GAC'GTC

Cuts at:    508     508 
   Size:       4217
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI
 Bme1580I     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NspI     PciI     PfoI     PmeI     PpiI     PsiI   PspOMI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AleI     AlfI     AloI     AscI    AvrII
     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI     BmtI     BpmI
    BsaXI     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII
  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     NcoI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PmlI    PpuMI    PshAI    PspXI     PsrI
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SwaI     XhoI
     XmnI
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