Restriction map for pF1KB8616
 (Linear) (Six-base) MAPSORT of: KIBB8616.seq  Check: 6325  from: 1  to: 1104



KIBB8616.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:29  ..

AatII G_ACGT'C

Cuts at:      0     402    1104 
   Size:        402     702
 
Acc65I G'GTAC_C

Cuts at:      0     483    1104 
   Size:        483     621
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     518     919    1031    1104 
   Size:        413     105     401     112      73

  Fragments arranged by size: 

                 413     401     112     105      73
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1086    1104 
   Size:       1086      18
 
ApaI G_GGCC'C

Cuts at:      0     460    1104 
   Size:        460     644
 
ApoI r'AATT_y

Cuts at:      0     115     300     742    1099    1104 
   Size:        115     185     442     357       5

  Fragments arranged by size: 

                 442     357     185     115       5
 
AvaI C'yCGr_G

Cuts at:      0      37    1104 
   Size:         37    1067
 
BanI G'GyrC_C

Cuts at:      0     483    1104 
   Size:        483     621
 
BanII G_rGCy'C

Cuts at:      0      29     460    1104 
   Size:         29     431     644

  Fragments arranged by size: 

                 644     431      29
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1037    1104 
   Size:       1037      67
 
BglII A'GATC_T

Cuts at:      0     149    1104 
   Size:        149     955
 
Bme1580I G_kGCm'C

Cuts at:      0     460     731    1104 
   Size:        460     271     373

  Fragments arranged by size: 

                 460     373     271
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     718    1104 
   Size:        718     386
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     824    1104 
   Size:        824     280
 
Bpu10I CC'TnA_GC

Cuts at:      0     250    1104 
   Size:        250     854
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     812    1104 
   Size:        812     292
 
BsaHI Gr'CG_yC

Cuts at:      0     399    1104 
   Size:        399     705
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     976    1104 
   Size:        976     128
 
BseYI C'CCAG_C

Cuts at:      0     459     731    1104 
   Size:        459     272     373

  Fragments arranged by size: 

                 459     373     272
 
BsiHKAI G_wGCw'C

Cuts at:      0       7    1104 
   Size:          7    1097
 
Bsp1286I G_dGCh'C

Cuts at:      0       7      29     460     731    1104 
   Size:          7      22     431     271     373

  Fragments arranged by size: 

                 431     373     271      22       7
 
BsrBI CCG'CTC

Cuts at:      0     828    1104 
   Size:        828     276
 
BsrDI GCAATG_nn'

Cuts at:      0     182     599     789    1104 
   Size:        182     417     190     315

  Fragments arranged by size: 

                 417     315     190     182
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     394     802    1104 
   Size:        394     408     302

  Fragments arranged by size: 

                 408     394     302
 
BstYI r'GATC_y

Cuts at:      0     149    1104 
   Size:        149     955
 
BtgI C'CryG_G

Cuts at:      0      15     517    1104 
   Size:         15     502     587

  Fragments arranged by size: 

                 587     502      15
 
DraI TTT'AAA

Cuts at:      0     747    1104 
   Size:        747     357
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     398    1104 
   Size:        398     706
 
EarI CTCTTCn'nnn_

Cuts at:      0     416     793     818    1104 
   Size:        416     377      25     286

  Fragments arranged by size: 

                 416     377     286      25
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     518     919    1031    1104 
   Size:        413     105     401     112      73

  Fragments arranged by size: 

                 413     401     112     105      73
 
EcoO109I rG'GnC_Cy

Cuts at:      0     456    1104 
   Size:        456     648
 
EcoRI G'AATT_C

Cuts at:      0     300    1099    1104 
   Size:        300     799       5

  Fragments arranged by size: 

                 799     300       5
 
FspI TGC'GCA

Cuts at:      0     106    1104 
   Size:        106     998
 
HaeII r_GCGC'y

Cuts at:      0      90    1104 
   Size:         90    1014
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     310     342    1014    1046    1072    1075    1104 
   Size:        310      32     672      32      26       3      29

  Fragments arranged by size: 

                 672     310      32      32      29      26       3
 
KpnI G_GTAC'C

Cuts at:      0     487    1104 
   Size:        487     617
 
MfeI C'AATT_G

Cuts at:      0     263    1104 
   Size:        263     841
 
MslI CAynn'nnrTG

Cuts at:      0     697     854    1104 
   Size:        697     157     250

  Fragments arranged by size: 

                 697     250     157
 
MspA1I CmG'CkG

Cuts at:      0      17      54     519     735     906    1104 
   Size:         17      37     465     216     171     198

  Fragments arranged by size: 

                 465     216     198     171      37      17
 
NdeI CA'TA_TG

Cuts at:      0     893    1104 
   Size:        893     211
 
PfoI T'CCnGG_A

Cuts at:      0     336    1104 
   Size:        336     768
 
PsiI TTA'TAA

Cuts at:      0    1028    1104 
   Size:       1028      76
 
PspOMI G'GGCC_C

Cuts at:      0     456    1104 
   Size:        456     648
 
PvuII CAG'CTG

Cuts at:      0     735     906    1104 
   Size:        735     171     198

  Fragments arranged by size: 

                 735     198     171
 
SacII CC_GC'GG

Cuts at:      0      18     520    1104 
   Size:         18     502     584

  Fragments arranged by size: 

                 584     502      18
 
SapI GCTCTTCn'nnn_

Cuts at:      0     818    1104 
   Size:        818     286
 
ScaI AGT'ACT

Cuts at:      0     642    1104 
   Size:        642     462
 
SfcI C'TryA_G

Cuts at:      0     350    1104 
   Size:        350     754
 
SpeI A'CTAG_T

Cuts at:      0     330    1104 
   Size:        330     774
 
SspI AAT'ATT

Cuts at:      0    1011    1104 
   Size:       1011      93
 
StuI AGG'CCT

Cuts at:      0     659    1104 
   Size:        659     445
 
StyI C'CwwG_G

Cuts at:      0     609     926    1104 
   Size:        609     317     178

  Fragments arranged by size: 

                 609     317     178
 
TatI w'GTAC_w

Cuts at:      0     440     640    1104 
   Size:        440     200     464

  Fragments arranged by size: 

                 464     440     200
 
ZraI GAC'GTC

Cuts at:      0     400    1104 
   Size:        400     704
 

 Enzymes that do cut:

    AatII   Acc65I     AcuI     AhdI     ApaI     ApoI     AvaI     BanI
    BanII     BbsI    BglII Bme1580I     BmrI     BplI   Bpu10I     BsaI
    BsaHI    BseRI    BseYI  BsiHKAI Bsp1286I    BsrBI    BsrDI    BstXI
    BstYI     BtgI     DraI     DrdI     EarI  Eco57MI EcoO109I    EcoRI
     FspI    HaeII    Hin4I     KpnI     MfeI     MslI   MspA1I     NdeI
     PfoI     PsiI   PspOMI    PvuII    SacII     SapI     ScaI     SfcI
     SpeI     SspI     StuI     StyI     TatI     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AclI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI    BmgBI     BmtI     BpmI    BpuEI    BsaAI    BsaBI
    BsaWI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI
    FspAI   HincII  HindIII     HpaI     KasI     MluI     MmeI     MscI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
     SalI    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SphI     SrfI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
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