Restriction map for pF1KB6557
 (Linear) (Six-base) MAPSORT of: KIBB6557.seq  Check: 8876  from: 1  to: 1116



KIBB6557.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     296    1116 
   Size:        296     820
 
AccI GT'mk_AC

Cuts at:      0      59    1116 
   Size:         59    1057
 
AhdI GACnn_n'nnGTC

Cuts at:      0     705    1116 
   Size:        705     411
 
AleI CACnn'nnGTG

Cuts at:      0     601    1116 
   Size:        601     515
 
BanI G'GyrC_C

Cuts at:      0      87     284     978    1041    1116 
   Size:         87     197     694      63      75

  Fragments arranged by size: 

                 694     197      87      75      63
 
BanII G_rGCy'C

Cuts at:      0     118    1116 
   Size:        118     998
 
BglI GCCn_nnn'nGGC

Cuts at:      0     251    1116 
   Size:        251     865
 
BglII A'GATC_T

Cuts at:      0     998    1116 
   Size:        998     118
 
BlpI GC'TnA_GC

Cuts at:      0     340    1116 
   Size:        340     776
 
Bme1580I G_kGCm'C

Cuts at:      0     647    1116 
   Size:        647     469
 
BmgBI CAC'GTC

Cuts at:      0     123     167     674    1116 
   Size:        123      44     507     442

  Fragments arranged by size: 

                 507     442     123      44
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     159     250    1116 
   Size:        159      91     866

  Fragments arranged by size: 

                 866     159      91
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     602     685     897    1116 
   Size:        602      83     212     219

  Fragments arranged by size: 

                 602     219     212      83
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     703    1116 
   Size:        463     240     413

  Fragments arranged by size: 

                 463     413     240
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     540     943    1116 
   Size:        540     403     173

  Fragments arranged by size: 

                 540     403     173
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     262     292     499     520     529     550     658     688 
   Size:        262      30     207      21       9      21     108      30

Cuts at:    688    1116 
   Size:        428

  Fragments arranged by size: 

                 428     262     207     108      30      30      21      21
                   9
 
BseYI C'CCAG_C

Cuts at:      0     471     646    1017    1116 
   Size:        471     175     371      99

  Fragments arranged by size: 

                 471     371     175      99
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     118    1116 
   Size:        118     998
 
BsiHKAI G_wGCw'C

Cuts at:      0     227     341    1116 
   Size:        227     114     775

  Fragments arranged by size: 

                 775     227     114
 
Bsp1286I G_dGCh'C

Cuts at:      0     118     227     341     647    1116 
   Size:        118     109     114     306     469

  Fragments arranged by size: 

                 469     306     118     114     109
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     209     296    1116 
   Size:        209      87     820

  Fragments arranged by size: 

                 820     209      87
 
BsrBI CCG'CTC

Cuts at:      0     350    1116 
   Size:        350     766
 
BsrDI GCAATG_nn'

Cuts at:      0     558    1116 
   Size:        558     558
 
BsrFI r'CCGG_y

Cuts at:      0     659    1116 
   Size:        659     457
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     453     975    1071    1116 
   Size:        453     522      96      45

  Fragments arranged by size: 

                 522     453      96      45
 
BstYI r'GATC_y

Cuts at:      0     998    1116 
   Size:        998     118
 
BtgI C'CryG_G

Cuts at:      0      16     228     325     929    1116 
   Size:         16     212      97     604     187

  Fragments arranged by size: 

                 604     212     187      97      16
 
BtsI GCAGTG_nn'

Cuts at:      0     127    1094    1116 
   Size:        127     967      22

  Fragments arranged by size: 

                 967     127      22
 
EaeI y'GGCC_r

Cuts at:      0     637    1116 
   Size:        637     479
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     602     685     897    1116 
   Size:        602      83     212     219

  Fragments arranged by size: 

                 602     219     212      83
 
EcoO109I rG'GnC_Cy

Cuts at:      0     468    1033    1116 
   Size:        468     565      83

  Fragments arranged by size: 

                 565     468      83
 
HaeII r_GCGC'y

Cuts at:      0     111    1116 
   Size:        111    1005
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      60      92     691     723     731     763    1116 
   Size:         60      32     599      32       8      32     353

  Fragments arranged by size: 

                 599     353      60      32      32      32       8
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     377     803    1116 
   Size:        377     426     313

  Fragments arranged by size: 

                 426     377     313
 
MslI CAynn'nnrTG

Cuts at:      0     132     601    1116 
   Size:        132     469     515

  Fragments arranged by size: 

                 515     469     132
 
MspA1I CmG'CkG

Cuts at:      0     219     327     344     383     488    1025    1087    1116 
   Size:        219     108      17      39     105     537      62      29

  Fragments arranged by size: 

                 537     219     108     105      62      39      29      17
            
 
NaeI GCC'GGC

Cuts at:      0     661    1116 
   Size:        661     455
 
NcoI C'CATG_G

Cuts at:      0     228    1116 
   Size:        228     888
 
NgoMIV G'CCGG_C

Cuts at:      0     659    1116 
   Size:        659     457
 
NspI r_CATG'y

Cuts at:      0     313     507     769    1116 
   Size:        313     194     262     347

  Fragments arranged by size: 

                 347     313     262     194
 
PasI CC'CwG_GG

Cuts at:      0     558    1009    1116 
   Size:        558     451     107

  Fragments arranged by size: 

                 558     451     107
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     234    1116 
   Size:        234     882
 
PfoI T'CCnGG_A

Cuts at:      0     749    1116 
   Size:        749     367
 
PpuMI rG'GwC_Cy

Cuts at:      0     468    1033    1116 
   Size:        468     565      83

  Fragments arranged by size: 

                 565     468      83
 
PstI C_TGCA'G

Cuts at:      0     382    1116 
   Size:        382     734
 
PvuII CAG'CTG

Cuts at:      0     219     344     383    1116 
   Size:        219     125      39     733

  Fragments arranged by size: 

                 733     219     125      39
 
SacII CC_GC'GG

Cuts at:      0     328    1116 
   Size:        328     788
 
SanDI GG'GwC_CC

Cuts at:      0    1033    1116 
   Size:       1033      83
 
SfcI C'TryA_G

Cuts at:      0     378    1116 
   Size:        378     738
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     251    1116 
   Size:        251     865
 
SmlI C'TyrA_G

Cuts at:      0     478     718    1116 
   Size:        478     240     398

  Fragments arranged by size: 

                 478     398     240
 
StyI C'CwwG_G

Cuts at:      0     228     365     845    1116 
   Size:        228     137     480     271

  Fragments arranged by size: 

                 480     271     228     137
 
TatI w'GTAC_w

Cuts at:      0     527     824    1116 
   Size:        527     297     292

  Fragments arranged by size: 

                 527     297     292
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     262     294     393     425     499     531     658     690 
   Size:        262      32      99      32      74      32     127      32

Cuts at:    690     855     887    1116 
   Size:        165      32     229

  Fragments arranged by size: 

                 262     229     165     127      99      74      32      32
                  32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0      74    1116 
   Size:         74    1042
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     972    1116 
   Size:        972     144
 

 Enzymes that do cut:

     AarI     AccI     AhdI     AleI     BanI    BanII     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BpmI    BpuEI     BsaI    BsaXI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BstXI    BstYI     BtgI     BtsI     EaeI  Eco57MI EcoO109I    HaeII
    Hin4I     MmeI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI
     PasI    PflMI     PfoI    PpuMI     PstI    PvuII    SacII    SanDI
     SfcI     SfiI     SmlI     StyI     TatI     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BmtI     BplI   Bpu10I    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NarI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI     SalI     SapI
     SbfI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     XbaI
     XhoI     XmaI     XmnI     ZraI
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