Restriction map for pF1KB5349
 (Circular) (Six-base) MAPSORT of: pF1KB5349.seq  Check: 9650  from: 1  to: 4241



pF1KB5349  4241 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AccI GT'mk_AC

Cuts at:   1279    1279 
   Size:       4241
 
Acc65I G'GTAC_C

Cuts at:   1257    1257 
   Size:       4241
 
AclI AA'CG_TT

Cuts at:   4081    4081 
   Size:       4241
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    208     880    2066    2498    3245    3513     208 
   Size:        672    1186     432     747     268     936

  Fragments arranged by size: 

                1186     936     747     672     432     268
 
AflIII A'CryG_T

Cuts at:    614    1593    2697    3674     614 
   Size:        979    1104     977    1181

  Fragments arranged by size: 

                1181    1104     979     977
 
AgeI A'CCGG_T

Cuts at:   2629    2629 
   Size:       4241
 
AleI CACnn'nnGTG

Cuts at:   1183    1183 
   Size:       4241
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1216    1216 
   Size:       4241
 
AlwNI CAG_nnn'CTG

Cuts at:    679     804     858     912    1546    3113     679 
   Size:        125      54      54     634    1567    1807

  Fragments arranged by size: 

                1807    1567     634     125      54      54
 
ApaI G_GGCC'C

Cuts at:    155     155 
   Size:       4241
 
ApaLI G'TGCA_C

Cuts at:   1176    3011    1176 
   Size:       1835    2406
 
ApoI r'AATT_y

Cuts at:    304     920    1245    3594    3850     304 
   Size:        616     325    2349     256     695

  Fragments arranged by size: 

                2349     695     616     325     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4241
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4241
 
AvaI C'yCGr_G

Cuts at:    154    1121    1261     154 
   Size:        967     140    3134

  Fragments arranged by size: 

                3134     967     140
 
BamHI G'GATC_C

Cuts at:    246    1266     246 
   Size:       1020    3221
 
BanI G'GyrC_C

Cuts at:    173    1257    1671    1919    1954     173 
   Size:       1084     414     248      35    2460

  Fragments arranged by size: 

                2460    1084     414     248      35
 
BanII G_rGCy'C

Cuts at:    148     155     191     468    1255    2285     148 
   Size:          7      36     277     787    1030    2104

  Fragments arranged by size: 

                2104    1030     787     277      36       7
 
BbeI G_GCGC'C

Cuts at:   1675    1923    1675 
   Size:        248    3993
 
BbsI GAAGACnn'nnnn_

Cuts at:    868     868 
   Size:       4241
 
BciVI GTATCCnnnnn_n'

Cuts at:   2139    2900    4241    2139 
   Size:        761    1341    2139

  Fragments arranged by size: 

                2139    1341     761
 
BclI T'GATC_A

Cuts at:   1761    1761 
   Size:       4241
 
BglII A'GATC_T

Cuts at:   1756    3793    1756 
   Size:       2037    2204
 
BlpI GC'TnA_GC

Cuts at:   1353    1353 
   Size:       4241
 
Bme1580I G_kGCm'C

Cuts at:    155    1180    1866    1959    3015     155 
   Size:       1025     686      93    1056    1381

  Fragments arranged by size: 

                1381    1056    1025     686      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1453    1534    1869    4013    1453 
   Size:         81     335    2144    1681

  Fragments arranged by size: 

                2144    1681     335      81
 
BmtI G_CTAG'C

Cuts at:    715     715 
   Size:       4241
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    289     772    2608     289 
   Size:        483    1836    1922

  Fragments arranged by size: 

                1922    1836     483
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    202     567     793    1186     202 
   Size:        365     226     393    3257

  Fragments arranged by size: 

                3257     393     365     226
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1417    2296    2788    3086    1417 
   Size:        879     492     298    2572

  Fragments arranged by size: 

                2572     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    277    3945     277 
   Size:       3668     573
 
BsaAI yAC'GTr

Cuts at:    354    1594     354 
   Size:       1240    3001
 
BsaBI GATnn'nnATC

Cuts at:      6    1755    3792       6 
   Size:       1749    2037     455

  Fragments arranged by size: 

                2037    1749     455
 
BsaHI Gr'CG_yC

Cuts at:   1672    1920    2622    4118    1672 
   Size:        248     702    1496    1795

  Fragments arranged by size: 

                1795    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1430    1951    2629    2903    3050    3461    1430 
   Size:        521     678     274     147     411    2210

  Fragments arranged by size: 

                2210     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1159    1189    1159 
   Size:         30    4211
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    199     745     199 
   Size:        546    3695
 
BseYI C'CCAG_C

Cuts at:    705     905    1666    2424    3001    3497     705 
   Size:        200     761     758     577     496    1449

  Fragments arranged by size: 

                1449     761     758     577     496     200
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    262    1135     262 
   Size:        873    3368
 
BsiEI CG_ry'CG

Cuts at:    104    1783    1829    3037     104 
   Size:       1679      46    1208    1308

  Fragments arranged by size: 

                1679    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    148     191     468    1180    1214    1255    2033    2223    3015 
   Size:         43     277     712      34      41     778     190     792

Cuts at:   3015     148 
   Size:       1374

  Fragments arranged by size: 

                1374     792     778     712     277     190      43      41
                  34
 
Bsp1286I G_dGCh'C

Cuts at:    148     155     191     468    1180    1214    1255    1866    1959 
   Size:          7      36     277     712      34      41     611      93

Cuts at:   1959    2033    2223    2285    3015     148 
   Size:         74     190      62     730    1374

  Fragments arranged by size: 

                1374     730     712     611     277     190      93      74
                  62      41      36      34       7
 
BspEI T'CCGG_A

Cuts at:   3461    3461 
   Size:       4241
 
BspHI T'CATG_A

Cuts at:   3417    3417 
   Size:       4241
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    969    1291    1505    1807    2188     969 
   Size:        322     214     302     381    3022

  Fragments arranged by size: 

                3022     381     322     302     214
 
BsrBI CCG'CTC

Cuts at:     50     846    2533    2587    4071    4236      50 
   Size:        796    1687      54    1484     165      55

  Fragments arranged by size: 

                1687    1484     796     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2153    2153 
   Size:       4241
 
BsrFI r'CCGG_y

Cuts at:    794    2239    2420    2629     794 
   Size:       1445     181     209    2406

  Fragments arranged by size: 

                2406    1445     209     181
 
BssSI C'ACGA_G

Cuts at:    334     407    2512    2870     334 
   Size:         73    2105     358    1705

  Fragments arranged by size: 

                2105    1705     358      73
 
BstAPI GCAn_nnn'nTGC

Cuts at:    768    1618     768 
   Size:        850    3391
 
BstBI TT'CG_AA

Cuts at:   2602    2602 
   Size:       4241
 
BstXI CCAn_nnnn'nTGG

Cuts at:    268     268 
   Size:       4241
 
BstYI r'GATC_y

Cuts at:      1     246     666    1266    1733    1756    2091    2337    3349 
   Size:        245     420     600     467      23     335     246    1012

Cuts at:   3349    3435    3447    3793       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     600     467     449     420     346     335     246
                 245      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    781     781 
   Size:       4241
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2191    2372    2535    2191 
   Size:        181     163    3897

  Fragments arranged by size: 

                3897     181     163
 
BtsI GCAGTG_nn'

Cuts at:    443     805    1484     443 
   Size:        362     679    3200

  Fragments arranged by size: 

                3200     679     362
 
DraI TTT'AAA

Cuts at:   1240    1240 
   Size:       4241
 
DraIII CAC_nnn'GTG

Cuts at:    318     768     318 
   Size:        450    3791
 
DrdI GACnn_nn'nnGTC

Cuts at:   1096    1947    2805    1096 
   Size:        851     858    2532

  Fragments arranged by size: 

                2532     858     851
 
EaeI y'GGCC_r

Cuts at:   1826    2000    2391    2418    3763    1826 
   Size:        174     391      27    1345    2304

  Fragments arranged by size: 

                2304    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1826    1826 
   Size:       4241
 
EarI CTCTTCn'nnn_

Cuts at:   1183    2264    2474    1183 
   Size:       1081     210    2950

  Fragments arranged by size: 

                2950    1081     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2449    2759    2905    4051    2449 
   Size:        310     146    1146    2639

  Fragments arranged by size: 

                2639    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    146     189     466    1253     146 
   Size:         43     277     787    3134

  Fragments arranged by size: 

                3134     787     277      43
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    202     208     567     793     880    1186    2066    2498    3245 
   Size:          6     359     226      87     306     880     432     747

Cuts at:   3245    3513     202 
   Size:        268     930

  Fragments arranged by size: 

                 930     880     747     432     359     306     268     226
                  87       6
 
EcoO109I rG'GnC_Cy

Cuts at:    486    1047    1380     486 
   Size:        561     333    3347

  Fragments arranged by size: 

                3347     561     333
 
EcoRI G'AATT_C

Cuts at:   1245    1245 
   Size:       4241
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    925    2260     925 
   Size:       1335    2906
 
FspI TGC'GCA

Cuts at:   2022    2022 
   Size:       4241
 
HaeII r_GCGC'y

Cuts at:   1675    1923    2945    3924    1675 
   Size:        248    1022     979    1992

  Fragments arranged by size: 

                1992    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    357     389    2083    2113    2115    2145     357 
   Size:         32    1694      30       2      30    2453

  Fragments arranged by size: 

                2453    1694      32      30      30       2
 
HincII GTy'rAC

Cuts at:    657    1280     657 
   Size:        623    3618
 
HindIII A'AGCT_T

Cuts at:    280     280 
   Size:       4241
 
KasI G'GCGC_C

Cuts at:   1671    1919    1671 
   Size:        248    3993
 
KpnI G_GTAC'C

Cuts at:   1261    1261 
   Size:       4241
 
MfeI C'AATT_G

Cuts at:    458     509     458 
   Size:         51    4190
 
MluI A'CGCG_T

Cuts at:    614    3674     614 
   Size:       3060    1181
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1109    2912    3096    1109 
   Size:       1803     184    2254

  Fragments arranged by size: 

                2254    1803     184
 
MscI TGG'CCA

Cuts at:   2002    2002 
   Size:       4241
 
MslI CAynn'nnrTG

Cuts at:    943    1183    1198    2230    2357     943 
   Size:        240      15    1032     127    2827

  Fragments arranged by size: 

                2827    1032     240     127      15
 
MspA1I CmG'CkG

Cuts at:    166     216    1352    1666    2026    3039    3284     166 
   Size:         50    1136     314     360    1013     245    1123

  Fragments arranged by size: 

                1136    1123    1013     360     314     245      50
 
NaeI GCC'GGC

Cuts at:   2422    2422 
   Size:       4241
 
NarI GG'CG_CC

Cuts at:   1672    1920    1672 
   Size:        248    3993
 
NgoMIV G'CCGG_C

Cuts at:   2420    2420 
   Size:       4241
 
NheI G'CTAG_C

Cuts at:    711     711 
   Size:       4241
 
NspI r_CATG'y

Cuts at:   1294    1794    2701    1294 
   Size:        500     907    2834

  Fragments arranged by size: 

                2834     907     500
 
PciI A'CATG_T

Cuts at:   2697    2697 
   Size:       4241
 
PmeI GTTT'AAAC

Cuts at:   1240    1240 
   Size:       4241
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3380    3412    3380 
   Size:         32    4209
 
PpuMI rG'GwC_Cy

Cuts at:    486     486 
   Size:       4241
 
PspOMI G'GGCC_C

Cuts at:    151     151 
   Size:       4241
 
PstI C_TGCA'G

Cuts at:   1288    1973    1288 
   Size:        685    3556
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4241
 
PvuII CAG'CTG

Cuts at:    216    1666    2026     216 
   Size:       1450     360    2431

  Fragments arranged by size: 

                2431    1450     360
 
RsrII CG'GwC_CG

Cuts at:   2436    2436 
   Size:       4241
 
SacI G_AGCT'C

Cuts at:    148     191     468    1255     148 
   Size:         43     277     787    3134

  Fragments arranged by size: 

                3134     787     277      43
 
SalI G'TCGA_C

Cuts at:   1278    1278 
   Size:       4241
 
SapI GCTCTTCn'nnn_

Cuts at:   1183    2264    2474    1183 
   Size:       1081     210    2950

  Fragments arranged by size: 

                2950    1081     210
 
SbfI CC_TGCA'GG

Cuts at:   1288    1288 
   Size:       4241
 
ScaI AGT'ACT

Cuts at:   3758    3758 
   Size:       4241
 
SfcI C'TryA_G

Cuts at:     33     130     490    1284    1969    2962    3153      33 
   Size:         97     360     794     685     993     191    1121

  Fragments arranged by size: 

                1121     993     794     685     360     191      97
 
SfoI GGC'GCC

Cuts at:   1673    1921    1673 
   Size:        248    3993
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4241
 
SmaI CCC'GGG

Cuts at:    156    1263     156 
   Size:       1107    3134
 
SmlI C'TyrA_G

Cuts at:   1396    2311    2803    3065    1396 
   Size:        915     492     262    2572

  Fragments arranged by size: 

                2572     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4241
 
SphI G_CATG'C

Cuts at:   1294    1794    1294 
   Size:        500    3741
 
SrfI GCCC'GGGC

Cuts at:    156     156 
   Size:       4241
 
StyI C'CwwG_G

Cuts at:    261    1375    1729     261 
   Size:       1114     354    2773

  Fragments arranged by size: 

                2773    1114     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1101    1824    1957    2416    2625    1101 
   Size:        723     133     459     209    2717

  Fragments arranged by size: 

                2717     723     459     209     133
 
TatI w'GTAC_w

Cuts at:   3756    3756 
   Size:       4241
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1159    1191    3758    3790    1159 
   Size:         32    2567      32    1610

  Fragments arranged by size: 

                2567    1610      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2038    2038 
   Size:       4241
 
XbaI T'CTAG_A

Cuts at:   1272    1272 
   Size:       4241
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1736    1736 
   Size:       4241
 
XmaI C'CCGG_G

Cuts at:    154    1261     154 
   Size:       1107    3134
 
XmnI GAAnn'nnTTC

Cuts at:    344     344 
   Size:       4241
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BciVI     BclI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I    BtgZI     BtsI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NheI     NspI     PciI     PmeI
     PpiI    PpuMI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     SrfI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AloI     AscI    AvrII
     BaeI    BbvCI     BcgI    BfrBI     BglI    BmgBI   Bpu10I    BsiWI
     BsmI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I     BtgI     ClaI
    CspCI    EcoNI    EcoRV     FseI    FspAI     HpaI     NcoI     NdeI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmlI
    PshAI     PsiI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SspI     StuI     SwaI     XhoI     ZraI
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