(Circular) (Six-base) MAPSORT of: pF1KB5349.seq Check: 9650 from: 1 to: 4241 pF1KB5349 4241 bp With 182 enzymes: SgfI * October 7, 2009 14:16 .. AccI GT'mk_AC Cuts at: 1279 1279 Size: 4241 Acc65I G'GTAC_C Cuts at: 1257 1257 Size: 4241 AclI AA'CG_TT Cuts at: 4081 4081 Size: 4241 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 208 880 2066 2498 3245 3513 208 Size: 672 1186 432 747 268 936 Fragments arranged by size: 1186 936 747 672 432 268 AflIII A'CryG_T Cuts at: 614 1593 2697 3674 614 Size: 979 1104 977 1181 Fragments arranged by size: 1181 1104 979 977 AgeI A'CCGG_T Cuts at: 2629 2629 Size: 4241 AleI CACnn'nnGTG Cuts at: 1183 1183 Size: 4241 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1216 1216 Size: 4241 AlwNI CAG_nnn'CTG Cuts at: 679 804 858 912 1546 3113 679 Size: 125 54 54 634 1567 1807 Fragments arranged by size: 1807 1567 634 125 54 54 ApaI G_GGCC'C Cuts at: 155 155 Size: 4241 ApaLI G'TGCA_C Cuts at: 1176 3011 1176 Size: 1835 2406 ApoI r'AATT_y Cuts at: 304 920 1245 3594 3850 304 Size: 616 325 2349 256 695 Fragments arranged by size: 2349 695 616 325 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4241 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4241 AvaI C'yCGr_G Cuts at: 154 1121 1261 154 Size: 967 140 3134 Fragments arranged by size: 3134 967 140 BamHI G'GATC_C Cuts at: 246 1266 246 Size: 1020 3221 BanI G'GyrC_C Cuts at: 173 1257 1671 1919 1954 173 Size: 1084 414 248 35 2460 Fragments arranged by size: 2460 1084 414 248 35 BanII G_rGCy'C Cuts at: 148 155 191 468 1255 2285 148 Size: 7 36 277 787 1030 2104 Fragments arranged by size: 2104 1030 787 277 36 7 BbeI G_GCGC'C Cuts at: 1675 1923 1675 Size: 248 3993 BbsI GAAGACnn'nnnn_ Cuts at: 868 868 Size: 4241 BciVI GTATCCnnnnn_n' Cuts at: 2139 2900 4241 2139 Size: 761 1341 2139 Fragments arranged by size: 2139 1341 761 BclI T'GATC_A Cuts at: 1761 1761 Size: 4241 BglII A'GATC_T Cuts at: 1756 3793 1756 Size: 2037 2204 BlpI GC'TnA_GC Cuts at: 1353 1353 Size: 4241 Bme1580I G_kGCm'C Cuts at: 155 1180 1866 1959 3015 155 Size: 1025 686 93 1056 1381 Fragments arranged by size: 1381 1056 1025 686 93 BmrI ACTGGGnnnn_n' Cuts at: 1453 1534 1869 4013 1453 Size: 81 335 2144 1681 Fragments arranged by size: 2144 1681 335 81 BmtI G_CTAG'C Cuts at: 715 715 Size: 4241 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 289 772 2608 289 Size: 483 1836 1922 Fragments arranged by size: 1922 1836 483 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 202 567 793 1186 202 Size: 365 226 393 3257 Fragments arranged by size: 3257 393 365 226 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1417 2296 2788 3086 1417 Size: 879 492 298 2572 Fragments arranged by size: 2572 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 277 3945 277 Size: 3668 573 BsaAI yAC'GTr Cuts at: 354 1594 354 Size: 1240 3001 BsaBI GATnn'nnATC Cuts at: 6 1755 3792 6 Size: 1749 2037 455 Fragments arranged by size: 2037 1749 455 BsaHI Gr'CG_yC Cuts at: 1672 1920 2622 4118 1672 Size: 248 702 1496 1795 Fragments arranged by size: 1795 1496 702 248 BsaWI w'CCGG_w Cuts at: 1430 1951 2629 2903 3050 3461 1430 Size: 521 678 274 147 411 2210 Fragments arranged by size: 2210 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1159 1189 1159 Size: 30 4211 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 199 745 199 Size: 546 3695 BseYI C'CCAG_C Cuts at: 705 905 1666 2424 3001 3497 705 Size: 200 761 758 577 496 1449 Fragments arranged by size: 1449 761 758 577 496 200 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 262 1135 262 Size: 873 3368 BsiEI CG_ry'CG Cuts at: 104 1783 1829 3037 104 Size: 1679 46 1208 1308 Fragments arranged by size: 1679 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 148 191 468 1180 1214 1255 2033 2223 3015 Size: 43 277 712 34 41 778 190 792 Cuts at: 3015 148 Size: 1374 Fragments arranged by size: 1374 792 778 712 277 190 43 41 34 Bsp1286I G_dGCh'C Cuts at: 148 155 191 468 1180 1214 1255 1866 1959 Size: 7 36 277 712 34 41 611 93 Cuts at: 1959 2033 2223 2285 3015 148 Size: 74 190 62 730 1374 Fragments arranged by size: 1374 730 712 611 277 190 93 74 62 41 36 34 7 BspEI T'CCGG_A Cuts at: 3461 3461 Size: 4241 BspHI T'CATG_A Cuts at: 3417 3417 Size: 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 969 1291 1505 1807 2188 969 Size: 322 214 302 381 3022 Fragments arranged by size: 3022 381 322 302 214 BsrBI CCG'CTC Cuts at: 50 846 2533 2587 4071 4236 50 Size: 796 1687 54 1484 165 55 Fragments arranged by size: 1687 1484 796 165 55 54 BsrDI GCAATG_nn' Cuts at: 2153 2153 Size: 4241 BsrFI r'CCGG_y Cuts at: 794 2239 2420 2629 794 Size: 1445 181 209 2406 Fragments arranged by size: 2406 1445 209 181 BssSI C'ACGA_G Cuts at: 334 407 2512 2870 334 Size: 73 2105 358 1705 Fragments arranged by size: 2105 1705 358 73 BstAPI GCAn_nnn'nTGC Cuts at: 768 1618 768 Size: 850 3391 BstBI TT'CG_AA Cuts at: 2602 2602 Size: 4241 BstXI CCAn_nnnn'nTGG Cuts at: 268 268 Size: 4241 BstYI r'GATC_y Cuts at: 1 246 666 1266 1733 1756 2091 2337 3349 Size: 245 420 600 467 23 335 246 1012 Cuts at: 3349 3435 3447 3793 1 Size: 86 12 346 449 Fragments arranged by size: 1012 600 467 449 420 346 335 246 245 86 23 12 Bsu36I CC'TnA_GG Cuts at: 781 781 Size: 4241 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2191 2372 2535 2191 Size: 181 163 3897 Fragments arranged by size: 3897 181 163 BtsI GCAGTG_nn' Cuts at: 443 805 1484 443 Size: 362 679 3200 Fragments arranged by size: 3200 679 362 DraI TTT'AAA Cuts at: 1240 1240 Size: 4241 DraIII CAC_nnn'GTG Cuts at: 318 768 318 Size: 450 3791 DrdI GACnn_nn'nnGTC Cuts at: 1096 1947 2805 1096 Size: 851 858 2532 Fragments arranged by size: 2532 858 851 EaeI y'GGCC_r Cuts at: 1826 2000 2391 2418 3763 1826 Size: 174 391 27 1345 2304 Fragments arranged by size: 2304 1345 391 174 27 EagI C'GGCC_G Cuts at: 1826 1826 Size: 4241 EarI CTCTTCn'nnn_ Cuts at: 1183 2264 2474 1183 Size: 1081 210 2950 Fragments arranged by size: 2950 1081 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2449 2759 2905 4051 2449 Size: 310 146 1146 2639 Fragments arranged by size: 2639 1146 310 146 EcoICRI GAG'CTC Cuts at: 146 189 466 1253 146 Size: 43 277 787 3134 Fragments arranged by size: 3134 787 277 43 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 202 208 567 793 880 1186 2066 2498 3245 Size: 6 359 226 87 306 880 432 747 Cuts at: 3245 3513 202 Size: 268 930 Fragments arranged by size: 930 880 747 432 359 306 268 226 87 6 EcoO109I rG'GnC_Cy Cuts at: 486 1047 1380 486 Size: 561 333 3347 Fragments arranged by size: 3347 561 333 EcoRI G'AATT_C Cuts at: 1245 1245 Size: 4241 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 925 2260 925 Size: 1335 2906 FspI TGC'GCA Cuts at: 2022 2022 Size: 4241 HaeII r_GCGC'y Cuts at: 1675 1923 2945 3924 1675 Size: 248 1022 979 1992 Fragments arranged by size: 1992 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 357 389 2083 2113 2115 2145 357 Size: 32 1694 30 2 30 2453 Fragments arranged by size: 2453 1694 32 30 30 2 HincII GTy'rAC Cuts at: 657 1280 657 Size: 623 3618 HindIII A'AGCT_T Cuts at: 280 280 Size: 4241 KasI G'GCGC_C Cuts at: 1671 1919 1671 Size: 248 3993 KpnI G_GTAC'C Cuts at: 1261 1261 Size: 4241 MfeI C'AATT_G Cuts at: 458 509 458 Size: 51 4190 MluI A'CGCG_T Cuts at: 614 3674 614 Size: 3060 1181 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1109 2912 3096 1109 Size: 1803 184 2254 Fragments arranged by size: 2254 1803 184 MscI TGG'CCA Cuts at: 2002 2002 Size: 4241 MslI CAynn'nnrTG Cuts at: 943 1183 1198 2230 2357 943 Size: 240 15 1032 127 2827 Fragments arranged by size: 2827 1032 240 127 15 MspA1I CmG'CkG Cuts at: 166 216 1352 1666 2026 3039 3284 166 Size: 50 1136 314 360 1013 245 1123 Fragments arranged by size: 1136 1123 1013 360 314 245 50 NaeI GCC'GGC Cuts at: 2422 2422 Size: 4241 NarI GG'CG_CC Cuts at: 1672 1920 1672 Size: 248 3993 NgoMIV G'CCGG_C Cuts at: 2420 2420 Size: 4241 NheI G'CTAG_C Cuts at: 711 711 Size: 4241 NspI r_CATG'y Cuts at: 1294 1794 2701 1294 Size: 500 907 2834 Fragments arranged by size: 2834 907 500 PciI A'CATG_T Cuts at: 2697 2697 Size: 4241 PmeI GTTT'AAAC Cuts at: 1240 1240 Size: 4241 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3380 3412 3380 Size: 32 4209 PpuMI rG'GwC_Cy Cuts at: 486 486 Size: 4241 PspOMI G'GGCC_C Cuts at: 151 151 Size: 4241 PstI C_TGCA'G Cuts at: 1288 1973 1288 Size: 685 3556 PvuI CG_AT'CG Cuts at: 104 104 Size: 4241 PvuII CAG'CTG Cuts at: 216 1666 2026 216 Size: 1450 360 2431 Fragments arranged by size: 2431 1450 360 RsrII CG'GwC_CG Cuts at: 2436 2436 Size: 4241 SacI G_AGCT'C Cuts at: 148 191 468 1255 148 Size: 43 277 787 3134 Fragments arranged by size: 3134 787 277 43 SalI G'TCGA_C Cuts at: 1278 1278 Size: 4241 SapI GCTCTTCn'nnn_ Cuts at: 1183 2264 2474 1183 Size: 1081 210 2950 Fragments arranged by size: 2950 1081 210 SbfI CC_TGCA'GG Cuts at: 1288 1288 Size: 4241 ScaI AGT'ACT Cuts at: 3758 3758 Size: 4241 SfcI C'TryA_G Cuts at: 33 130 490 1284 1969 2962 3153 33 Size: 97 360 794 685 993 191 1121 Fragments arranged by size: 1121 993 794 685 360 191 97 SfoI GGC'GCC Cuts at: 1673 1921 1673 Size: 248 3993 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4241 SmaI CCC'GGG Cuts at: 156 1263 156 Size: 1107 3134 SmlI C'TyrA_G Cuts at: 1396 2311 2803 3065 1396 Size: 915 492 262 2572 Fragments arranged by size: 2572 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4241 SphI G_CATG'C Cuts at: 1294 1794 1294 Size: 500 3741 SrfI GCCC'GGGC Cuts at: 156 156 Size: 4241 StyI C'CwwG_G Cuts at: 261 1375 1729 261 Size: 1114 354 2773 Fragments arranged by size: 2773 1114 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1101 1824 1957 2416 2625 1101 Size: 723 133 459 209 2717 Fragments arranged by size: 2717 723 459 209 133 TatI w'GTAC_w Cuts at: 3756 3756 Size: 4241 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1159 1191 3758 3790 1159 Size: 32 2567 32 1610 Fragments arranged by size: 2567 1610 32 32 Tth111I GACn'n_nGTC Cuts at: 2038 2038 Size: 4241 XbaI T'CTAG_A Cuts at: 1272 1272 Size: 4241 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1736 1736 Size: 4241 XmaI C'CCGG_G Cuts at: 154 1261 154 Size: 1107 3134 XmnI GAAnn'nnTTC Cuts at: 344 344 Size: 4241 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI NspI PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AloI AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I BtgI ClaI CspCI EcoNI EcoRV FseI FspAI HpaI NcoI NdeI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SspI StuI SwaI XhoI ZraI