Restriction map for pF1KE0745
 (Linear) (Six-base) MAPSORT of: KIEE0745.seq  Check: 6469  from: 1  to: 960



KIEE0745.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AccI GT'mk_AC

Cuts at:      0     565     960 
   Size:        565     395
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     237     960 
   Size:        237     723
 
AflIII A'CryG_T

Cuts at:      0     748     960 
   Size:        748     212
 
ApaI G_GGCC'C

Cuts at:      0     530     960 
   Size:        530     430
 
BamHI G'GATC_C

Cuts at:      0      98     960 
   Size:         98     862
 
BanII G_rGCy'C

Cuts at:      0      89     153     530     773     960 
   Size:         89      64     377     243     187

  Fragments arranged by size: 

                 377     243     187      89      64
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     859     893     960 
   Size:        859      34      67

  Fragments arranged by size: 

                 859      67      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     510     960 
   Size:        510     450
 
Bme1580I G_kGCm'C

Cuts at:      0     530     960 
   Size:        530     430
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      23     960 
   Size:         23     937
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     960 
   Size:         29     931
 
Bpu10I CC'TnA_GC

Cuts at:      0     812     960 
   Size:        812     148
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      76     430     960 
   Size:         76     354     530

  Fragments arranged by size: 

                 530     354      76
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     256     748     960 
   Size:        256     492     212

  Fragments arranged by size: 

                 492     256     212
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      64      67      79     436     439     960 
   Size:         64       3      12     357       3     521

  Fragments arranged by size: 

                 521     357      64      12       3       3
 
BseYI C'CCAG_C

Cuts at:      0     522     555     960 
   Size:        522      33     405

  Fragments arranged by size: 

                 522     405      33
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628     960 
   Size:        628     332
 
BsiHKAI G_wGCw'C

Cuts at:      0      89     153     773     960 
   Size:         89      64     620     187

  Fragments arranged by size: 

                 620     187      89      64
 
Bsp1286I G_dGCh'C

Cuts at:      0      89     153     530     773     960 
   Size:         89      64     377     243     187

  Fragments arranged by size: 

                 377     243     187      89      64
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     770     960 
   Size:        770     190
 
BsrDI GCAATG_nn'

Cuts at:      0     887     960 
   Size:        887      73
 
BsrFI r'CCGG_y

Cuts at:      0     603     960 
   Size:        603     357
 
BstBI TT'CG_AA

Cuts at:      0      22     960 
   Size:         22     938
 
BstYI r'GATC_y

Cuts at:      0      98     960 
   Size:         98     862
 
BtgI C'CryG_G

Cuts at:      0     672     960 
   Size:        672     288
 
BtsI GCAGTG_nn'

Cuts at:      0     154     960 
   Size:        154     806
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      73     960 
   Size:         73     887
 
EaeI y'GGCC_r

Cuts at:      0     107     350     545     828     960 
   Size:        107     243     195     283     132

  Fragments arranged by size: 

                 283     243     195     132     107
 
EarI CTCTTCn'nnn_

Cuts at:      0     778     960 
   Size:        778     182
 
EcoICRI GAG'CTC

Cuts at:      0      87     151     771     960 
   Size:         87      64     620     189

  Fragments arranged by size: 

                 620     189      87      64
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     237     960 
   Size:         29     208     723

  Fragments arranged by size: 

                 723     208      29
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     811     960 
   Size:        811     149
 
EcoO109I rG'GnC_Cy

Cuts at:      0     405     429     526     960 
   Size:        405      24      97     434

  Fragments arranged by size: 

                 434     405      97      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     361     393     960 
   Size:        361      32     567

  Fragments arranged by size: 

                 567     361      32
 
MluI A'CGCG_T

Cuts at:      0     748     960 
   Size:        748     212
 
MscI TGG'CCA

Cuts at:      0     109     352     830     960 
   Size:        109     243     478     130

  Fragments arranged by size: 

                 478     243     130     109
 
MspA1I CmG'CkG

Cuts at:      0     503     555     674     960 
   Size:        503      52     119     286

  Fragments arranged by size: 

                 503     286     119      52
 
PpuMI rG'GwC_Cy

Cuts at:      0     405     429     960 
   Size:        405      24     531

  Fragments arranged by size: 

                 531     405      24
 
PspOMI G'GGCC_C

Cuts at:      0     526     960 
   Size:        526     434
 
PstI C_TGCA'G

Cuts at:      0     767     960 
   Size:        767     193
 
PvuII CAG'CTG

Cuts at:      0     555     960 
   Size:        555     405
 
SacI G_AGCT'C

Cuts at:      0      89     153     773     960 
   Size:         89      64     620     187

  Fragments arranged by size: 

                 620     187      89      64
 
SacII CC_GC'GG

Cuts at:      0     675     960 
   Size:        675     285
 
SapI GCTCTTCn'nnn_

Cuts at:      0     778     960 
   Size:        778     182
 
SbfI CC_TGCA'GG

Cuts at:      0     767     960 
   Size:        767     193
 
SexAI A'CCwGG_T

Cuts at:      0      41     960 
   Size:         41     919
 
SfcI C'TryA_G

Cuts at:      0     763     960 
   Size:        763     197
 
SmlI C'TyrA_G

Cuts at:      0      91     445     960 
   Size:         91     354     515

  Fragments arranged by size: 

                 515     354      91
 
StyI C'CwwG_G

Cuts at:      0     167     836     960 
   Size:        167     669     124

  Fragments arranged by size: 

                 669     167     124
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     772     960 
   Size:        772     188
 
TatI w'GTAC_w

Cuts at:      0     206     960 
   Size:        206     754
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     332     364     960 
   Size:        332      32     596

  Fragments arranged by size: 

                 596     332      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     373     960 
   Size:        373     587
 
XmnI GAAnn'nnTTC

Cuts at:      0     310     960 
   Size:        310     650
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     ApaI    BamHI    BanII     BcgI    BciVI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspMI    BsrDI    BsrFI    BstBI    BstYI
     BtgI     BtsI     DrdI     EaeI     EarI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    Hin4I     MluI     MscI   MspA1I    PpuMI   PspOMI     PstI
    PvuII     SacI    SacII     SapI     SbfI    SexAI     SfcI     SmlI
     StyI    TaqII     TatI     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI     BbsI    BbvCI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrGI   BssHII    BssSI   BstAPI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     EagI     EciI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MmeI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SalI    SanDI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     XbaI     XcmI     XhoI     XmaI     ZraI
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