Restriction map for pF1KB6909
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbsI        BbvCI       BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BpmI        BpuAI       BsaHI       BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrDI       BsrGI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     ClaI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        Hin1I       
HindIII     HpaI        KasI        KspI        MfeI        
MluI        MroI        MseI        MslI        MunI        
NarI        NcoI        NdeI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tru9I       
Tsp509I     Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

Acc65I         65  GGTACC               G^GTACC
AfaI           66  GTAC                 GT^AC
AlwI          261  GATCC                GGATC(4/5)
AocI          475  CCTGAGG              CC^T[ACGT]AGG
Asp718I        65  GGTACC               G^GTACC
BalI          151  TGGCCA               TGG^CCA
BanI           65  GGTACC               G^G[CT][GA]CC
BcgI          597  CGATGAGACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          514  CTAG                 C^TAG
BlpI          228  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      228  GCTCAGC              GC^T[ACGT]AGC
BsaAI         160  TACGTG               [CT]AC^GT[GA]
BsaBI         534  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          252  GGTCTC               GGTCTC(1/5)
BsrBI         376  GAGCGG               CCGCTC(-3/-3)
BssHII        224  GCGCGC               G^CGCGC
BssSI         370  CACGAG               CACGAG(-5/-1)
BstYI         260  AGATCC               [GA]^GATC[CT]
Bsu36I        475  CCTGAGG              CC^T[ACGT]AGG
CelII         228  GCTCAGC              GC^T[ACGT]AGC
CpoI          275  CGGACCG              CG^G[AT]CCG
DsaI          491  CCGTGG               C^C[GA][CT]GG
EarI           82  CTCTTC               CTCTTC(1/4)
Eco81I        475  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       338  CCAAGG               C^C[AT][AT]GG
HaeII           8  AGCGCC               [GA]GCGC^[CT]
HgaI           14  GCGTC                GACGC(5/10)
HincII        301  GTCAAC               GT[CT]^[GA]AC
HindII        301  GTCAAC               GT[CT]^[GA]AC
HinfI         556  GAGTC                G^A[ACGT]TC
KpnI           65  GGTACC               GGTAC^C
Ksp632I        82  CTCTTC               CTCTTC(1/4)
MaeI          514  CTAG                 C^TAG
MaeII         161  ACGT                 A^CGT
MaeIII        547  GTCAC                ^GT[ACGT]AC
MamI          534  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII          83  TCTTC                GAAGA(8/7)
MflI          260  AGATCC               [GA]^GATC[CT]
MluNI         151  TGGCCA               TGG^CCA
MscI          151  TGGCCA               TGG^CCA
NaeI          233  GCCGGC               GCC^GGC
NgoMIV        233  GCCGGC               G^CCGGC
NheI          513  GCTAGC               G^CTAGC
PleI          556  GAGTC                GAGTC(4/5)
PpuMI          47  GGGACCC              [GA]G^G[AT]CC[CT]
RsaI           66  GTAC                 GT^AC
RsrII         275  CGGACCG              CG^G[AT]CCG
SfcI          216  CTACAG               C^T[GA][CT]AG
StuI           92  AGGCCT               AGG^CCT
StyI          338  CCAAGG               C^C[AT][AT]GG
Tsp45I        547  GTCAC                ^GT[GC]AC
XcmI          338  CCAAGGATGGCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         260  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AlwNI         144  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
              581  CAGAAGCTG           
BsmAI         601  GAGAC                GTCTC(1/5)
              253  GTCTC               
BsrFI         233  GCCGGC               [GA]^CCGG[CT]
              361  ACCGGC              
BsrI           62  ACTGG                ACTGG(1/-1)
              544  CCAGT               
Cfr10I        233  GCCGGC               [GA]^CCGG[CT]
              361  ACCGGC              
EaeI          151  TGGCCA               [CT]^GGCC[GA]
              203  TGGCCG              
NlaIII        391  CATG                 CATG^
              504  CATG                
PvuII          42  CAGCTG               CAG^CTG
              147  CAGCTG              


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AccII       AciI        AluI        ApaI        AvaII       
BanII       BbvI        BcnI        BmyI        BsaJI       
BseRI       BsiYI       BslI        BsmFI       Bsp1286I    
BssKI       BstF5I      BstNI       BstUI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EcoO109I    EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HpaII       
HphI        ItaI        MboI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIV       Sau3AI      Sau96I      ScrFI       
TaqI        TseI        TspRI       TthHB8I     
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