Restriction map for pF1KB8538
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AgeI        AhdI        AlwNI       
AocI        Aor51HI     ApaI        AscI        Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanII       BbeI        BbrPI       
BbvCI       BcgI        BcnI        BlnI        BsaHI       
BsaWI       BseAI       BsgI        BsiWI       BsmBI       
BsoBI       BspDI       BspEI       BsrBI       BsrDI       
BssHII      BssSI       Bst1107I    BstUI       BstZ17I     
Bsu36I      ClaI        CpoI        DraII       DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoRV       FauI        FseI        
FspI        HaeII       Hin1I       HincII      HindII      
HpaI        KasI        KpnI        KspI        MluI        
MluNI       MroI        MscI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    StuI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AflII         976  CTTAAG               C^TTAAG
Alw44I       2415  GTGCAC               G^TGCAC
ApaLI        2415  GTGCAC               G^TGCAC
AseI         1464  ATTAAT               AT^TAAT
AsnI         1464  ATTAAT               AT^TAAT
AvaII           9  GGACC                G^G[AT]CC
BanI          472  GGTGCC               G^G[CT][GA]CC
BciVI         150  GTATCC               GTATCC(6/5)
BfrI          976  CTTAAG               C^TTAAG
BglI         2206  GCCACATGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1767  AGATCT               A^GATCT
BlpI         1060  GCTCAGC              GC^T[ACGT]AGC
BpmI         1928  CTCCAG               CTGGAG(16/14)
Bpu1102I     1060  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1449  CACGTA               [CT]AC^GT[GA]
BsaBI        1762  GATAAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          596  GGTCTC               GGTCTC(1/5)
BsiEI        1045  CGATCG               CG[GA][CT]^CG
BsmI         2461  GCATTC               GAATGC(1/-1)
Bsp1407I     1558  TGTACA               T^GTACA
BspMI        1634  ACCTGC               ACCTGC(4/8)
BsrFI          11  ACCGGC               [GA]^CCGG[CT]
BsrGI        1558  TGTACA               T^GTACA
BstBI         758  TTCGAA               TT^CGAA
BstEII       2098  GGTGACC              G^GT[ACGT]ACC
BstPI        2098  GGTGACC              G^GT[ACGT]ACC
BstXI        2231  CCATTCAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        1060  GCTCAGC              GC^T[ACGT]AGC
CfoI         1485  GCGC                 GCG^C
Cfr10I         11  ACCGGC               [GA]^CCGG[CT]
DraIII       1423  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         2466  CCGTGG               C^C[GA][CT]GG
EaeI         1030  CGGCCA               [CT]^GGCC[GA]
EcoO65I      2098  GGTGACC              G^GT[ACGT]ACC
EcoRI        2182  GAATTC               G^AATTC
EcoT14I       380  CCAAGG               C^C[AT][AT]GG
EcoT22I       784  ATGCAT               ATGCA^T
HapII          12  CCGG                 C^CGG
HgaI         2670  GCGTC                GACGC(5/10)
HhaI         1485  GCGC                 GCG^C
HinP1I       1485  GCGC                 G^CGC
HindIII      2111  AAGCTT               A^AGCTT
HpaII          12  CCGG                 C^CGG
MamI         1762  GATAAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI         2232  CATTCAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           12  CCGG                 C^CGG
NsiI          784  ATGCAT               ATGCA^T
NspV          758  TTCGAA               TT^CGAA
PshBI        1464  ATTAAT               AT^TAAT
PvuI         1045  CGATCG               CGAT^CG
ScaI          950  AGTACT               AGT^ACT
SfuI          758  TTCGAA               TT^CGAA
SspBI        1558  TGTACA               T^GTACA
StyI          380  CCAAGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AciI         1413  CCGC                 CCGC(-3/-1)
             1441  CCGC                
AspHI         734  GAGCAC               G[AT]GC[AT]^C
             2415  GTGCAC              
BbsI          994  GAAGAC               GAAGAC(2/6)
             1875  GAAGAC              
BclI          362  TGATCA               T^GATCA
             2483  TGATCA              
BfaI         1543  CTAG                 C^TAG
             1867  CTAG                
BpuAI         994  GAAGAC               GAAGAC(2/6)
             1875  GAAGAC              
BsiHKAI       734  GAGCAC               G[AT]GC[AT]^C
             2415  GTGCAC              
BspHI         545  TCATGA               T^CATGA
             1248  TCATGA              
BspLU11I     1745  ACATGT               A^CATGT
             2576  ACATGT              
BstAPI         63  GCAAATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              466  GCATTTGGTGC         
BstYI        1767  AGATCT               [GA]^GATC[CT]
             2588  AGATCC              
Cfr13I          9  GGACC                G^G[ACGT]CC
             2382  GGCCC               
EcoNI         972  CCTACTTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1542  CCTAGAAGAGG         
FbaI          362  TGATCA               T^GATCA
             2483  TGATCA              
MaeI         1543  CTAG                 C^TAG
             1867  CTAG                
MflI         1767  AGATCT               [GA]^GATC[CT]
             2588  AGATCC              
MspA1I         73  CAGCTG               C[AC]G^C[GT]G
             2005  CAGCTG              
PvuII          73  CAGCTG               CAG^CTG
             2005  CAGCTG              
RcaI          545  TCATGA               T^CATGA
             1248  TCATGA              
Sau96I          9  GGACC                G^G[ACGT]CC
             2382  GGCCC               
SphI         1307  GCATGC               GCATG^C
             2048  GCATGC              
SspI          316  AATATT               AAT^ATT
             2157  AATATT              
Tsp45I        323  GTGAC                ^GT[GC]AC
             2099  GTGAC               
XhoII        1767  AGATCT               [GA]^GATC[CT]
             2588  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AcsI        AfaI        AflIII      AluI        AlwI        
ApoI        Asp700I     BbvI        BmyI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       DdeI        DpnII       DraI        EarI        
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MfeI        MnlI        MseI        
MunI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        RsaI        
Sau3AI      ScrFI       SfaNI       SfcI        SmlI        
TaqI        TfiI        Tru9I       TseI        Tsp509I     
TspRI       TthHB8I     XmnI        
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