Restriction map for pF1KB9957
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       AccII       AflII       AflIII      AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaJI       
BseRI       BsgI        BsiEI       BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspLU11I    BsrBI       
BsrGI       BssHII      BssSI       BstEII      BstPI       
BstUI       Bsu36I      Cac8I       CelII       ClaI        
CpoI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FseI        FspI        HgaI        HincII      HindII      
HpaI        KspI        MamI        MluI        MluNI       
MscI        MspA1I      MvnI        NaeI        NciI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       PvuI        PvuII       RsrII       SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tth111I     XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

Acc65I       1257  GGTACC               G^GTACC
AccIII        443  TCCGGA               T^CCGGA
AclI         1106  AACGTT               AA^CGTT
AcyI         1028  GGCGCC               G[GA]^CG[CT]C
AgeI          101  ACCGGT               A^CCGGT
AlwI         1317  GATCC                GGATC(4/5)
Asp700I       727  GAAGTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1257  GGTACC               G^GTACC
BbeI         1028  GGCGCC               GGCGC^C
BsaHI        1028  GGCGCC               G[GA]^CG[CT]C
BseAI         443  TCCGGA               T^CCGGA
BsmFI         130  GGGAC                GGGAC(10/14)
BsmI          359  GCATTC               GAATGC(1/-1)
BspEI         443  TCCGGA               T^CCGGA
BspHI         627  TCATGA               T^CATGA
BsrFI         101  ACCGGT               [GA]^CCGG[CT]
BssKI         886  CCAGG                ^CC[ACGT]GG
BstAPI       1054  GCATTGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1589  TTCGAA               TT^CGAA
BstNI         886  CCAGG                CC^[AT]GG
BstXI        1348  CCAATGATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        101  ACCGGT               [GA]^CCGG[CT]
DraI          334  TTTAAA               TTT^AAA
EarI         1343  CTCTTC               CTCTTC(1/4)
EcoNI         441  CCTCCGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        886  CCAGG                ^CC[AT]GG
HaeII        1028  GGCGCC               [GA]GCGC^[CT]
Hin1I        1028  GGCGCC               G[GA]^CG[CT]C
HindIII       176  AAGCTT               A^AGCTT
HphI         1517  TCACC                GGTGA(8/7)
KasI         1028  GGCGCC               G^GCGCC
KpnI         1257  GGTACC               GGTAC^C
Ksp632I      1343  CTCTTC               CTCTTC(1/4)
MaeII        1107  ACGT                 A^CGT
MfeI          587  CAATTG               C^AATTG
MroI          443  TCCGGA               T^CCGGA
MslI           81  CACTGACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          587  CAATTG               C^AATTG
MvaI          886  CCAGG                CC^[AT]GG
NarI         1028  GGCGCC               GG^CGCC
NspV         1589  TTCGAA               TT^CGAA
PflMI        1321  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         101  ACCGGT               A^CCGGT
PleI         1311  GAGTC                GAGTC(4/5)
Psp1406I     1106  AACGTT               AA^CGTT
PstI         1187  CTGCAG               CTGCA^G
RcaI          627  TCATGA               T^CATGA
SacI         1574  GAGCTC               GAGCT^C
ScaI          876  AGTACT               AGT^ACT
ScrFI         886  CCAGG                CC^[ACGT]GG
SfaNI        1060  GATGC                GCATC(5/9)
SfoI         1028  GGCGCC               GGC^GCC
SfuI         1589  TTCGAA               TT^CGAA
Van91I       1321  CCAGCAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          727  GAAGTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI          953  GTATAC               GT^[AC][GT]AC
             1163  GTATAC              
AlwNI          25  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1181  CAGTTGCTG           
AspHI         303  GTGCTC               G[AT]GC[AT]^C
             1574  GAGCTC              
BanI         1028  GGCGCC               G^G[CT][GA]CC
             1257  GGTACC              
BanII        1358  GGGCTC               G[GA]GC[CT]^C
             1574  GAGCTC              
BbsI         1365  GAAGAC               GAAGAC(2/6)
             1387  GTCTTC              
BglII         399  AGATCT               A^GATCT
              540  AGATCT              
BpuAI        1365  GAAGAC               GAAGAC(2/6)
             1387  GTCTTC              
BsaWI         101  ACCGGT               [AT]^CCGG[AT]
              443  TCCGGA              
BsiHKAI       303  GTGCTC               G[AT]GC[AT]^C
             1574  GAGCTC              
BspMI        1377  ACCTGC               ACCTGC(4/8)
             1324  GCAGGT              
BsrDI        1521  CATTGC               GCAATG(2/0)
             1192  GCAATG              
Bst1107I      953  GTATAC               GTA^TAC
             1163  GTATAC              
BstZ17I       953  GTATAC               GTA^TAC
             1163  GTATAC              
CfoI          708  GCGC                 GCG^C
             1029  GCGC                
Cfr13I        159  GGGCC                G^G[ACGT]CC
              486  GGCCC               
HaeIII        160  GGCC                 GG^CC
              486  GGCC                
HapII         102  CCGG                 C^CGG
              444  CCGG                
HhaI          708  GCGC                 GCG^C
             1029  GCGC                
HinP1I        708  GCGC                 G^CGC
             1029  GCGC                
HpaII         102  CCGG                 C^CGG
              444  CCGG                
MspI          102  CCGG                 C^CGG
              444  CCGG                
MwoI          331  GCATTTAAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1054  GCATTGGATGC         
NlaIV        1028  GGCGCC               GG[ACGT]^[ACGT]CC
             1257  GGTACC              
NspI          425  GCATGT               [GA]CATG^[CT]
             1544  GCATGT              
Sau96I        159  GGGCC                G^G[ACGT]CC
              486  GGCCC               


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BbvI        BfaI        BmyI        BpmI        BsaI        
BsiYI       BslI        BsmAI       Bsp1286I    BsrI        
BstF5I      BstYI       DdeI        DpnII       Fnu4HI      
FokI        HinfI       ItaI        MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
NdeII       NlaIII      RsaI        Sau3AI      SfcI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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