Restriction map for pF1KE1083
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BlnI        BlpI        BpmI        Bpu1102I    BsaAI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsiYI       BslI        BsmI        
BsoBI       Bsp1407I    BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      CpoI        DraI        DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRV       
EcoT14I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MluI        
MluNI       MroI        MscI        MspI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
Sse8387I    SspBI       StuI        StyI        SwaI        
TfiI        Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AciI          747  CCGC                 CCGC(-3/-1)
AcsI          399  GAATTC               [GA]^AATT[CT]
AfaI          818  GTAC                 GT^AC
ApoI          399  GAATTC               [GA]^AATT[CT]
AseI          423  ATTAAT               AT^TAAT
AsnI          423  ATTAAT               AT^TAAT
Asp700I       835  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1059  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanII         692  GGGCTC               G[GA]GC[CT]^C
BbsI         1036  GAAGAC               GAAGAC(2/6)
BglII         741  AGATCT               A^GATCT
BpuAI        1036  GAAGAC               GAAGAC(2/6)
BsaBI         433  GATACATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI          89  CCGTGG               C^C[ACGT][ACGT]GG
BsmAI         849  GAGAC                GTCTC(1/5)
BsmBI         849  GAGACG               CGTCTC(1/5)
BsmFI         944  GGGAC                GGGAC(10/14)
BspDI         310  ATCGAT               AT^CGAT
BssKI         624  CCTGG                ^CC[ACGT]GG
BssSI          16  CACGAG               CACGAG(-5/-1)
BstNI         624  CCTGG                CC^[AT]GG
BstYI         741  AGATCT               [GA]^GATC[CT]
Cac8I         981  GCCAGC               GC[ACGT]^[ACGT]GC
ClaI          310  ATCGAT               AT^CGAT
DraII         944  GGGACCT              [GA]G^G[ACGT]CC[CT]
DraIII         35  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI           89  CCGTGG               C^C[GA][CT]GG
EcoO109I      944  GGGACCT              [GA]G^G[ACGT]CC[CT]
EcoRI         399  GAATTC               G^AATTC
EcoRII        624  CCTGG                ^CC[AT]GG
EcoT22I       556  ATGCAT               ATGCA^T
HaeIII        980  GGCC                 GG^CC
MaeII         178  ACGT                 A^CGT
MamI          433  GATACATATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          104  CAATTG               C^AATTG
MflI          741  AGATCT               [GA]^GATC[CT]
MunI          104  CAATTG               C^AATTG
MvaI          624  CCTGG                CC^[AT]GG
NsiI          556  ATGCAT               ATGCA^T
NspI         1040  ACATGC               [GA]CATG^[CT]
PflFI        1059  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         944  GGGACCT              [GA]G^G[AT]CC[CT]
PshBI         423  ATTAAT               AT^TAAT
PstI          532  CTGCAG               CTGCA^G
RsaI          818  GTAC                 GT^AC
SapI          835  GAAGAGC              GCTCTTC(1/4)
ScrFI         624  CCTGG                CC^[ACGT]GG
SfcI          532  CTGCAG               C^T[GA][CT]AG
SmlI          794  CTCAAG               C^T[CT][GA]AG
SpeI          160  ACTAGT               A^CTAGT
SspI           77  AATATT               AAT^ATT
Tsp45I       1057  GTGAC                ^GT[GC]AC
Tth111I      1059  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII         741  AGATCT               [GA]^GATC[CT]
XmnI          835  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AlwI          313  GATCC                GGATC(4/5)
              308  GGATC               
AspHI          41  GTGCTC               G[AT]GC[AT]^C
              998  GAGCAC              
BfaI          161  CTAG                 C^TAG
             1015  CTAG                
BsiHKAI        41  GTGCTC               G[AT]GC[AT]^C
              998  GAGCAC              
BsrDI          49  CATTGC               GCAATG(2/0)
              242  GCAATG              
BsrI          356  ACTGG                ACTGG(1/-1)
              989  CCAGT               
EarI          835  GAAGAG               CTCTTC(1/4)
              846  GAAGAG              
HinfI           4  GAGTC                G^A[ACGT]TC
              608  GAGTC               
HphI          499  GGTGA                GGTGA(8/7)
             1018  GGTGA               
Ksp632I       835  GAAGAG               CTCTTC(1/4)
              846  GAAGAG              
MaeI          161  CTAG                 C^TAG
             1015  CTAG                
MaeIII        157  GTTAC                ^GT[ACGT]AC
             1057  GTGAC               
MspA1I        115  CAGCTG               C[AC]G^C[GT]G
             1052  CAGCTG              
PleI            4  GAGTC                GAGTC(4/5)
              608  GAGTC               
PvuII         115  CAGCTG               CAG^CTG
             1052  CAGCTG              
TaqI          311  TCGA                 T^CGA
              561  TCGA                
TthHB8I       311  TCGA                 T^CGA
              561  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AluI        AvaII       BbvI        BmyI        BseRI       
Bsp1286I    BstF5I      Cfr13I      DdeI        DpnII       
Fnu4HI      FokI        ItaI        MboI        MboII       
MnlI        MseI        MslI        MwoI        NdeII       
NlaIII      NlaIV       Sau3AI      Sau96I      SfaNI       
Tru9I       TseI        Tsp509I     TspRI       
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