Restriction map for pF1KE1083
 (Circular) (Six-base) MAPSORT of: pF1KE1083.seq  Check: 8712  from: 1  to: 4223



pF1KE1083  4223 bp

 With 182 enzymes: SgfI * 

                        July 22, 2009 17:02  ..

AccI GT'mk_AC

Cuts at:   1261    1261 
   Size:       4223
 
Acc65I G'GTAC_C

Cuts at:   1239    1239 
   Size:       4223
 
AclI AA'CG_TT

Cuts at:   4063    4063 
   Size:       4223
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    923    1138    2048    2480    3227    3495     923 
   Size:        215     910     432     747     268    1651

  Fragments arranged by size: 

                1651     910     747     432     268     215
 
AflIII A'CryG_T

Cuts at:   1575    2679    3656    1575 
   Size:       1104     977    2142

  Fragments arranged by size: 

                2142    1104     977
 
AgeI A'CCGG_T

Cuts at:   2611    2611 
   Size:       4223
 
AlwNI CAG_nnn'CTG

Cuts at:   1528    3095    1528 
   Size:       1567    2656
 
ApaLI G'TGCA_C

Cuts at:   2993    2993 
   Size:       4223
 
ApoI r'AATT_y

Cuts at:    507    1227    3576    3832     507 
   Size:        720    2349     256     898

  Fragments arranged by size: 

                2349     898     720     256
 
AseI AT'TA_AT

Cuts at:     20     532      20 
   Size:        512    3711
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4223
 
AvaI C'yCGr_G

Cuts at:   1243    1243 
   Size:       4223
 
BamHI G'GATC_C

Cuts at:   1248    1248 
   Size:       4223
 
BanI G'GyrC_C

Cuts at:   1239    1653    1901    1936    1239 
   Size:        414     248      35    3526

  Fragments arranged by size: 

                3526     414     248      35
 
BanII G_rGCy'C

Cuts at:    804    1237    2267     804 
   Size:        433    1030    2760

  Fragments arranged by size: 

                2760    1030     433
 
BbeI G_GCGC'C

Cuts at:   1657    1905    1657 
   Size:        248    3975
 
BbsI GAAGACnn'nnnn_

Cuts at:   1151    1151 
   Size:       4223
 
BciVI GTATCCnnnnn_n'

Cuts at:   2121    2882    4223    2121 
   Size:        761    1341    2121

  Fragments arranged by size: 

                2121    1341     761
 
BclI T'GATC_A

Cuts at:   1743    1743 
   Size:       4223
 
BfrBI ATG'CAT

Cuts at:    666     666 
   Size:       4223
 
BglII A'GATC_T

Cuts at:    849    1738    3775     849 
   Size:        889    2037    1297

  Fragments arranged by size: 

                2037    1297     889
 
BlpI GC'TnA_GC

Cuts at:   1335    1335 
   Size:       4223
 
Bme1580I G_kGCm'C

Cuts at:   1848    1941    2997    1848 
   Size:         93    1056    3074

  Fragments arranged by size: 

                3074    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1435    1516    1851    3995    1435 
   Size:         81     335    2144    1663

  Fragments arranged by size: 

                2144    1663     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2590    2590 
   Size:       4223
 
Bpu10I CC'TnA_GC

Cuts at:    412     762     412 
   Size:        350    3873
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    887    1399    2278    2770    3068     887 
   Size:        512     879     492     298    2042

  Fragments arranged by size: 

                2042     879     512     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3927    3927 
   Size:       4223
 
BsaAI yAC'GTr

Cuts at:   1576    1576 
   Size:       4223
 
BsaBI GATnn'nnATC

Cuts at:      6     545    1737    3774       6 
   Size:        539    1192    2037     455

  Fragments arranged by size: 

                2037    1192     539     455
 
BsaHI Gr'CG_yC

Cuts at:   1654    1902    2604    4100    1654 
   Size:        248     702    1496    1777

  Fragments arranged by size: 

                1777    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1412    1933    2611    2885    3032    3443    1412 
   Size:        521     678     274     147     411    2192

  Fragments arranged by size: 

                2192     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    247     250     275     799     247 
   Size:          3      25     524    3671

  Fragments arranged by size: 

                3671     524      25       3
 
BseYI C'CCAG_C

Cuts at:   1648    2406    2983    3479    1648 
   Size:        758     577     496    2392

  Fragments arranged by size: 

                2392     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1765    1811    3019     104 
   Size:       1661      46    1208    1308

  Fragments arranged by size: 

                1661    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    153    1110    1237    2015    2205    2997     153 
   Size:        957     127     778     190     792    1379

  Fragments arranged by size: 

                1379     957     792     778     190     127
 
BsmBI CGTCTCn'nnnn_

Cuts at:    951     951 
   Size:       4223
 
Bsp1286I G_dGCh'C

Cuts at:    153     804    1110    1237    1848    1941    2015    2205    2267 
   Size:        651     306     127     611      93      74     190      62

Cuts at:   2267    2997     153 
   Size:        730    1379

  Fragments arranged by size: 

                1379     730     651     611     306     190     127      93
                  74      62
 
BspEI T'CCGG_A

Cuts at:   3443    3443 
   Size:       4223
 
BspHI T'CATG_A

Cuts at:   3399    3399 
   Size:       4223
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1273    1487    1789    2170    1273 
   Size:        214     302     381    3326

  Fragments arranged by size: 

                3326     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2515    2569    4053    4218      50 
   Size:       2465      54    1484     165      55

  Fragments arranged by size: 

                2465    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    156     357    2135     156 
   Size:        201    1778    2244

  Fragments arranged by size: 

                2244    1778     201
 
BsrFI r'CCGG_y

Cuts at:   2221    2402    2611    2221 
   Size:        181     209    3833

  Fragments arranged by size: 

                3833     209     181
 
BssSI C'ACGA_G

Cuts at:    124    2494    2852     124 
   Size:       2370     358    1495

  Fragments arranged by size: 

                2370    1495     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1600    1600 
   Size:       4223
 
BstBI TT'CG_AA

Cuts at:   2584    2584 
   Size:       4223
 
BstYI r'GATC_y

Cuts at:      1     849    1248    1715    1738    2073    2319    3331    3417 
   Size:        848     399     467      23     335     246    1012      86

Cuts at:   3417    3429    3775       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     848     467     449     399     346     335     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     197     107 
   Size:         90    4133
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2173    2354    2517    2173 
   Size:        181     163    3879

  Fragments arranged by size: 

                3879     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1466    1466 
   Size:       4223
 
ClaI AT'CG_AT

Cuts at:    419     419 
   Size:       4223
 
DraI TTT'AAA

Cuts at:   1222    1222 
   Size:       4223
 
DraIII CAC_nnn'GTG

Cuts at:    148     148 
   Size:       4223
 
DrdI GACnn_nn'nnGTC

Cuts at:   1929    2787    1929 
   Size:        858    3365
 
EaeI y'GGCC_r

Cuts at:   1808    1982    2373    2400    3745    1808 
   Size:        174     391      27    1345    2286

  Fragments arranged by size: 

                2286    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1808    1808 
   Size:       4223
 
EarI CTCTTCn'nnn_

Cuts at:    938     949    2246    2456     938 
   Size:         11    1297     210    2705

  Fragments arranged by size: 

                2705    1297     210      11
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2431    2741    2887    4033    2431 
   Size:        310     146    1146    2621

  Fragments arranged by size: 

                2621    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1235    1235 
   Size:       4223
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    923    1138    2048    2480    3227    3495     923 
   Size:        215     910     432     747     268    1651

  Fragments arranged by size: 

                1651     910     747     432     268     215
 
EcoO109I rG'GnC_Cy

Cuts at:   1053    1362    1053 
   Size:        309    3914
 
EcoRI G'AATT_C

Cuts at:    507    1227     507 
   Size:        720    3503
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    160    1168    2242     160 
   Size:       1008    1074    2141

  Fragments arranged by size: 

                2141    1074    1008
 
FspI TGC'GCA

Cuts at:   2004    2004 
   Size:       4223
 
HaeII r_GCGC'y

Cuts at:   1657    1905    2927    3906    1657 
   Size:        248    1022     979    1974

  Fragments arranged by size: 

                1974    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1059    1091    2065    2095    2097    2127    1059 
   Size:         32     974      30       2      30    3155

  Fragments arranged by size: 

                3155     974      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1262    1262 
   Size:       4223
 
KasI G'GCGC_C

Cuts at:   1653    1901    1653 
   Size:        248    3975
 
KpnI G_GTAC'C

Cuts at:   1243    1243 
   Size:       4223
 
MfeI C'AATT_G

Cuts at:    212     212 
   Size:       4223
 
MluI A'CGCG_T

Cuts at:   3656    3656 
   Size:       4223
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    681    2894    3078     681 
   Size:       2213     184    1826

  Fragments arranged by size: 

                2213    1826     184
 
MscI TGG'CCA

Cuts at:   1984    1984 
   Size:       4223
 
MslI CAynn'nnrTG

Cuts at:    444    1077    1079    2212    2339     444 
   Size:        633       2    1133     127    2328

  Fragments arranged by size: 

                2328    1133     633     127       2
 
MspA1I CmG'CkG

Cuts at:    225    1162    1334    1648    2008    3021    3266     225 
   Size:        937     172     314     360    1013     245    1182

  Fragments arranged by size: 

                1182    1013     937     360     314     245     172
 
NaeI GCC'GGC

Cuts at:   2404    2404 
   Size:       4223
 
NarI GG'CG_CC

Cuts at:   1654    1902    1654 
   Size:        248    3975
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4223
 
NgoMIV G'CCGG_C

Cuts at:   2402    2402 
   Size:       4223
 
NsiI A_TGCA'T

Cuts at:    668     668 
   Size:       4223
 
NspI r_CATG'y

Cuts at:   1152    1276    1776    2683    1152 
   Size:        124     500     907    2692

  Fragments arranged by size: 

                2692     907     500     124
 
PciI A'CATG_T

Cuts at:   2679    2679 
   Size:       4223
 
PmeI GTTT'AAAC

Cuts at:   1222    1222 
   Size:       4223
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3362    3394    3362 
   Size:         32    4191
 
PpuMI rG'GwC_Cy

Cuts at:   1053    1053 
   Size:       4223
 
PsiI TTA'TAA

Cuts at:    346     455     346 
   Size:        109    4114
 
PstI C_TGCA'G

Cuts at:    644    1270    1955     644 
   Size:        626     685    2912

  Fragments arranged by size: 

                2912     685     626
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4223
 
PvuII CAG'CTG

Cuts at:    225    1162    1648    2008     225 
   Size:        937     486     360    2440

  Fragments arranged by size: 

                2440     937     486     360
 
RsrII CG'GwC_CG

Cuts at:   2418    2418 
   Size:       4223
 
SacI G_AGCT'C

Cuts at:   1237    1237 
   Size:       4223
 
SalI G'TCGA_C

Cuts at:   1260    1260 
   Size:       4223
 
SapI GCTCTTCn'nnn_

Cuts at:    938    2246    2456     938 
   Size:       1308     210    2705

  Fragments arranged by size: 

                2705    1308     210
 
SbfI CC_TGCA'GG

Cuts at:   1270    1270 
   Size:       4223
 
ScaI AGT'ACT

Cuts at:   3740    3740 
   Size:       4223
 
SfcI C'TryA_G

Cuts at:     33     640    1266    1951    2944    3135      33 
   Size:        607     626     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     626     607     191
 
SfoI GGC'GCC

Cuts at:   1655    1903    1655 
   Size:        248    3975
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4223
 
SmaI CCC'GGG

Cuts at:   1245    1245 
   Size:       4223
 
SmlI C'TyrA_G

Cuts at:    902    1378    2293    2785    3047     902 
   Size:        476     915     492     262    2078

  Fragments arranged by size: 

                2078     915     492     476     262
 
SpeI A'CTAG_T

Cuts at:     67     268      67 
   Size:        201    4022
 
SphI G_CATG'C

Cuts at:   1276    1776    1276 
   Size:        500    3723
 
SspI AAT'ATT

Cuts at:    187     187 
   Size:       4223
 
StyI C'CwwG_G

Cuts at:    107    1357    1711     107 
   Size:       1250     354    2619

  Fragments arranged by size: 

                2619    1250     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1806    1939    2398    2607    1806 
   Size:        133     459     209    3422

  Fragments arranged by size: 

                3422     459     209     133
 
TatI w'GTAC_w

Cuts at:   3738    3738 
   Size:       4223
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3740    3772    3740 
   Size:         32    4191
 
Tth111I GACn'n_nGTC

Cuts at:   1170    2020    1170 
   Size:        850    3373
 
XbaI T'CTAG_A

Cuts at:   1254    1254 
   Size:       4223
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1718    1718 
   Size:       4223
 
XmaI C'CCGG_G

Cuts at:   1243    1243 
   Size:       4223
 
XmnI GAAnn'nnTTC

Cuts at:    947     947 
   Size:       4223
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I     BmrI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI
     BtgI    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NsiI     NspI     PciI     PmeI     PpiI    PpuMI     PsiI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI     BcgI     BglI    BmgBI
     BmtI     BpmI    BsaXI     BsgI    BsiWI     BsmI    BsrGI   BssHII
   BstEII    BstXI  BstZ17I   Bsu36I    CspCI    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     NdeI     NheI     NotI     NruI     PacI
     PasI    PflMI     PfoI     PmlI    PshAI   PspOMI    PspXI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     StuI
     SwaI     XhoI     ZraI
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