Restriction map for pF1KB8501
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        Asp718I     AspHI       AviII       
AvrII       BalI        BanI        BanII       BbeI        
BbrPI       BcgI        BfrI        BglI        BlnI        
BlpI        BmyI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BseAI       BseRI       BsiHKAI     BsiWI       
BsmBI       BsmI        Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspMI       BsrFI       BsrGI       
BssHII      BstBI       BstEII      BstPI       BstXI       
CelII       Cfr10I      ClaI        CpoI        DraIII      
DrdI        DsaI        EagI        EclXI       Eco47III    
Eco52I      EcoO65I     FseI        FspI        HaeII       
HgaI        Hin1I       HindIII     KasI        KpnI        
KspI        MamI        MluI        MluNI       MroI        
MscI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NspV        PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SwaI        Tsp45I      Van91I      XbaI        XcmI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccII         219  CGCG                 CG^CG
AflIII       2445  ACATGT               A^C[GA][CT]GT
AhdI         1843  GACACTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         170  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI           76  CCTCAGG              CC^T[ACGT]AGG
AspEI        1843  GACACTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         1225  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1601  CTCGAG               C^[CT]CG[GA]G
BbvCI           3  GCTGAGG              CCTCAGC(-5/-2)
BciVI        2409  GTATCC               GTATCC(6/5)
BglII          14  AGATCT               A^GATCT
BsaI          141  GGTCTC               GGTCTC(1/5)
BsaWI         185  ACCGGA               [AT]^CCGG[AT]
BsiEI         183  CGACCG               CG[GA][CT]^CG
BsoBI        1601  CTCGAG               C^[CT]CG[GA]G
BspLU11I     2445  ACATGT               A^CATGT
BsrBI          69  CCGCTC               CCGCTC(-3/-3)
BsrDI        1822  CATTGC               GCAATG(2/0)
BssSI         326  CACGAG               CACGAG(-5/-1)
Bst1107I     2484  GTATAC               GTA^TAC
BstAPI        829  GCAGCTGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         219  CGCG                 CG^CG
BstZ17I      2484  GTATAC               GTA^TAC
Bsu36I         76  CCTCAGG              CC^T[ACGT]AGG
CfoI          220  GCGC                 GCG^C
DraII          81  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EaeI          232  CGGCCA               [CT]^GGCC[GA]
Eam1105I     1843  GACACTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         2515  GAAGAG               CTCTTC(1/4)
Eco81I         76  CCTCAGG              CC^T[ACGT]AGG
EcoNI         795  CCTAATGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       81  GGGTCCC              [GA]G^G[ACGT]CC[CT]
HhaI          220  GCGC                 GCG^C
HinP1I        220  GCGC                 G^CGC
HincII       1093  GTTAAC               GT[CT]^[GA]AC
HindII       1093  GTTAAC               GT[CT]^[GA]AC
HpaI         1093  GTTAAC               GTT^AAC
Ksp632I      2515  GAAGAG               CTCTTC(1/4)
MaeII         166  ACGT                 A^CGT
MspA1I        830  CAGCTG               C[AC]G^C[GT]G
MvnI          219  CGCG                 CG^CG
PaeR7I       1601  CTCGAG               C^TCGAG
PflFI        1225  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI          81  GGGTCCC              [GA]G^G[AT]CC[CT]
PstI         2540  CTGCAG               CTGCA^G
PvuII         830  CAGCTG               CAG^CTG
ScaI         2170  AGTACT               AGT^ACT
SspI         2126  AATATT               AAT^ATT
StuI            7  AGGCCT               AGG^CCT
Tth111I      1225  GACACGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         1601  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

BamHI        1293  GGATCC               G^GATCC
             2712  GGATCC              
BbsI         1360  GAAGAC               GAAGAC(2/6)
             1571  GTCTTC              
BcnI           39  CCCGG                CC^[GC]GG
              230  CCCGG               
BpmI          103  CTCCAG               CTGGAG(16/14)
             1034  CTGGAG              
BpuAI        1360  GAAGAC               GAAGAC(2/6)
             1571  GTCTTC              
BsmFI        2505  GGGAC                GGGAC(10/14)
               83  GTCCC               
EcoRI         657  GAATTC               G^AATTC
              781  GAATTC              
EcoRV        1545  GATATC               GAT^ATC
             2182  GATATC              
EcoT22I       771  ATGCAT               ATGCA^T
             2428  ATGCAT              
MfeI         2567  CAATTG               C^AATTG
             2707  CAATTG              
MslI         1614  CATCCAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2395  CATATTGATG          
MunI         2567  CAATTG               C^AATTG
             2707  CAATTG              
NciI           39  CCCGG                CC^[GC]GG
              230  CCCGG               
NdeI          578  CATATG               CA^TATG
             2111  CATATG              
NsiI          771  ATGCAT               ATGCA^T
             2428  ATGCAT              
NspI         2426  ACATGC               [GA]CATG^[CT]
             2445  ACATGT              
PacI          967  TTAATTAA             TTAAT^TAA
             1720  TTAATTAA            


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        AseI        AsnI        Asp700I     
AvaII       BbvI        BclI        BfaI        BsaJI       
BsgI        BsiYI       BslI        BsmAI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraI        EcoRII      
EcoT14I     FauI        FbaI        Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
PshBI       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     XhoII       XmnI        
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