Restriction map for pF1KB8501
 (Circular) (Six-base) MAPSORT of: pF1KB8501.seq  Check: 7334  from: 1  to: 5921



pF1KB8501  5921 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AccI GT'mk_AC

Cuts at:    475    1152    2593    2959     475 
   Size:        677    1441     366    3437

  Fragments arranged by size: 

                3437    1441     677     366
 
Acc65I G'GTAC_C

Cuts at:   2937    2937 
   Size:       5921
 
AclI AA'CG_TT

Cuts at:   5761    5761 
   Size:       5921
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1024    1754    2930    3746    4178    4925    5193    1024 
   Size:        730    1176     816     432     747     268    1752

  Fragments arranged by size: 

                1752    1176     816     747     730     432     268
 
AflIII A'CryG_T

Cuts at:   2553    3273    4377    5354    2553 
   Size:        720    1104     977    3120

  Fragments arranged by size: 

                3120    1104     977     720
 
AgeI A'CCGG_T

Cuts at:   4309    4309 
   Size:       5921
 
AhdI GACnn_n'nnGTC

Cuts at:   1956    1956 
   Size:       5921
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2207    2239    2207 
   Size:         32    5889
 
AlwNI CAG_nnn'CTG

Cuts at:    283    3226    4793     283 
   Size:       2943    1567    1411

  Fragments arranged by size: 

                2943    1567    1411
 
ApaLI G'TGCA_C

Cuts at:   4691    4691 
   Size:       5921
 
ApoI r'AATT_y

Cuts at:    402     713     765     798     889     967     978    1319    1365 
   Size:        311      52      33      91      78      11     341      46

Cuts at:   1365    1594    1774    1800    2023    2301    2720    2925    5274 
   Size:        229     180      26     223     278     419     205    2349

Cuts at:   5274    5530     402 
   Size:        256     793

  Fragments arranged by size: 

                2349     793     419     341     311     278     256     229
                 223     205     180      91      78      52      46      33
                  26      11
 
AseI AT'TA_AT

Cuts at:     20    1079    1514    2377      20 
   Size:       1059     435     863    3564

  Fragments arranged by size: 

                3564    1059     863     435
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5921
 
AvaI C'yCGr_G

Cuts at:   1709    2941    1709 
   Size:       1232    4689
 
BamHI G'GATC_C

Cuts at:   1401    2820    2946    1401 
   Size:       1419     126    4376

  Fragments arranged by size: 

                4376    1419     126
 
BanI G'GyrC_C

Cuts at:   2937    3351    3599    3634    2937 
   Size:        414     248      35    5224

  Fragments arranged by size: 

                5224     414     248      35
 
BanII G_rGCy'C

Cuts at:   2935    3965    2935 
   Size:       1030    4891
 
BbeI G_GCGC'C

Cuts at:   3355    3603    3355 
   Size:        248    5673
 
BbsI GAAGACnn'nnnn_

Cuts at:   1475    1672    1475 
   Size:        197    5724
 
BbvCI CC'TCA_GC

Cuts at:    112     112 
   Size:       5921
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    5887
 
BciVI GTATCCnnnnn_n'

Cuts at:   2528    3819    4580    5921    2528 
   Size:       1291     761    1341    2528

  Fragments arranged by size: 

                2528    1341    1291     761
 
BclI T'GATC_A

Cuts at:    698     788    1758    2135    3441     698 
   Size:         90     970     377    1306    3178

  Fragments arranged by size: 

                3178    1306     970     377      90
 
BfrBI ATG'CAT

Cuts at:    881    2538     881 
   Size:       1657    4264
 
BglII A'GATC_T

Cuts at:    122    3436    5473     122 
   Size:       3314    2037     570

  Fragments arranged by size: 

                3314    2037     570
 
BlpI GC'TnA_GC

Cuts at:   3033    3033 
   Size:       5921
 
Bme1580I G_kGCm'C

Cuts at:   3546    3639    4695    3546 
   Size:         93    1056    4772

  Fragments arranged by size: 

                4772    1056      93
 
BmgBI CAC'GTC

Cuts at:    275     275 
   Size:       5921
 
BmrI ACTGGGnnnn_n'

Cuts at:    660    3133    3214    3549    5693     660 
   Size:       2473      81     335    2144     888

  Fragments arranged by size: 

                2473    2144     888     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4288    4288 
   Size:       5921
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    196    1163     196 
   Size:        967    4954
 
Bpu10I CC'TnA_GC

Cuts at:    112     112 
   Size:       5921
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    332    2553    2839    3097    3976    4468    4766     332 
   Size:       2221     286     258     879     492     298    1487

  Fragments arranged by size: 

                2221    1487     879     492     298     286     258
 
BsaI GGTCTCn'nnnn_

Cuts at:    255    5625     255 
   Size:       5370     551
 
BsaAI yAC'GTr

Cuts at:   3274    3274 
   Size:       5921
 
BsaBI GATnn'nnATC

Cuts at:      6    3435    5472       6 
   Size:       3429    2037     455

  Fragments arranged by size: 

                3429    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   3352    3600    4302    5798    3352 
   Size:        248     702    1496    3475

  Fragments arranged by size: 

                3475    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:    293    3110    3631    4309    4583    4730    5141     293 
   Size:       2817     521     678     274     147     411    1073

  Fragments arranged by size: 

                2817    1073     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    901     931    1122    1152     901 
   Size:         30     191      30    5670

  Fragments arranged by size: 

                5670     191      30      30
 
BseYI C'CCAG_C

Cuts at:   3346    4104    4681    5177    3346 
   Size:        758     577     496    4090

  Fragments arranged by size: 

                4090     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    416     542    1980     416 
   Size:        126    1438    4357

  Fragments arranged by size: 

                4357    1438     126
 
BsiEI CG_ry'CG

Cuts at:    104     294    3463    3509    4717     104 
   Size:        190    3169      46    1208    1308

  Fragments arranged by size: 

                3169    1308    1208     190      46
 
BsiHKAI G_wGCw'C

Cuts at:   2935    3713    3903    4695    2935 
   Size:        778     190     792    4161

  Fragments arranged by size: 

                4161     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:   2935    3546    3639    3713    3903    3965    4695    2935 
   Size:        611      93      74     190      62     730    4161

  Fragments arranged by size: 

                4161     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   5141    5141 
   Size:       5921
 
BspHI T'CATG_A

Cuts at:   5097    5097 
   Size:       5921
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   2971    3185    3487    3868    2971 
   Size:        214     302     381    5024

  Fragments arranged by size: 

                5024     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     179    4213    4267    5751    5916      50 
   Size:        129    4034      54    1484     165      55

  Fragments arranged by size: 

                4034    1484     165     129      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1929    3833    1929 
   Size:       1904    4017
 
BsrFI r'CCGG_y

Cuts at:   3919    4100    4309    3919 
   Size:        181     209    5531

  Fragments arranged by size: 

                5531     209     181
 
BssSI C'ACGA_G

Cuts at:    434    4192    4550     434 
   Size:       3758     358    1805

  Fragments arranged by size: 

                3758    1805     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    943    3298     943 
   Size:       2355    3566
 
BstBI TT'CG_AA

Cuts at:   4282    4282 
   Size:       5921
 
BstYI r'GATC_y

Cuts at:      1     122    1401    2820    2946    3413    3436    3771    4017 
   Size:        121    1279    1419     126     467      23     335     246

Cuts at:   4017    5029    5115    5127    5473       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1419    1279    1012     467     449     346     335     246
                 126     121      86      23      12
 
BstZ17I GTA'TAC

Cuts at:   2594    2594 
   Size:       5921
 
Bsu36I CC'TnA_GG

Cuts at:    185     185 
   Size:       5921
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   3871    4052    4215    3871 
   Size:        181     163    5577

  Fragments arranged by size: 

                5577     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1580    3164    1580 
   Size:       1584    4337
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   2772    2807    2772 
   Size:         35    5886
 
DraI TTT'AAA

Cuts at:    641    1409    2177    2920     641 
   Size:        768     768     743    3642

  Fragments arranged by size: 

                3642     768     768     743
 
DrdI GACnn_nn'nnGTC

Cuts at:   3627    4485    3627 
   Size:        858    5063
 
EaeI y'GGCC_r

Cuts at:    340    3506    3680    4071    4098    5443     340 
   Size:       3166     174     391      27    1345     818

  Fragments arranged by size: 

                3166    1345     818     391     174      27
 
EagI C'GGCC_G

Cuts at:   3506    3506 
   Size:       5921
 
EarI CTCTTCn'nnn_

Cuts at:   2618    3944    4154    2618 
   Size:       1326     210    4385

  Fragments arranged by size: 

                4385    1326     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4129    4439    4585    5731    4129 
   Size:        310     146    1146    4319

  Fragments arranged by size: 

                4319    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   2933    2933 
   Size:       5921
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    196    1024    1163    1754    2930    3746    4178    4925    5193 
   Size:        828     139     591    1176     816     432     747     268

Cuts at:   5193     196 
   Size:        924

  Fragments arranged by size: 

                1176     924     828     816     747     591     432     268
                 139
 
EcoNI CCTnn'n_nnAGG

Cuts at:    907     907 
   Size:       5921
 
EcoO109I rG'GnC_Cy

Cuts at:    190    3060     190 
   Size:       2870    3051
 
EcoRI G'AATT_C

Cuts at:    765     889    2925     765 
   Size:        124    2036    3761

  Fragments arranged by size: 

                3761    2036     124
 
EcoRV GAT'ATC

Cuts at:   1655    2292    1655 
   Size:        637    5284
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    443    1249    3940     443 
   Size:        806    2691    2424

  Fragments arranged by size: 

                2691    2424     806
 
FspI TGC'GCA

Cuts at:   3702    3702 
   Size:       5921
 
HaeII r_GCGC'y

Cuts at:   3355    3603    4625    5604    3355 
   Size:        248    1022     979    3672

  Fragments arranged by size: 

                3672    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    112     144     901     933    1036    1068    1706    1738    1942 
   Size:         32     757      32     103      32     638      32     204

Cuts at:   1942    1974    2644    2676    3763    3793    3795    3825     112 
   Size:         32     670      32    1087      30       2      30    2208

  Fragments arranged by size: 

                2208    1087     757     670     638     204     103      32
                  32      32      32      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1203    2960    1203 
   Size:       1757    4164
 
HpaI GTT'AAC

Cuts at:   1203    1203 
   Size:       5921
 
KasI G'GCGC_C

Cuts at:   3351    3599    3351 
   Size:        248    5673
 
KpnI G_GTAC'C

Cuts at:   2941    2941 
   Size:       5921
 
MfeI C'AATT_G

Cuts at:   2675    2815    2675 
   Size:        140    5781
 
MluI A'CGCG_T

Cuts at:   5354    5354 
   Size:       5921
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   4592    4776    4592 
   Size:        184    5737
 
MscI TGG'CCA

Cuts at:   3682    3682 
   Size:       5921
 
MslI CAynn'nnrTG

Cuts at:   1726    2507    3910    4037    1726 
   Size:        781    1403     127    3610

  Fragments arranged by size: 

                3610    1403     781     127
 
MspA1I CmG'CkG

Cuts at:    940    3032    3346    3706    4719    4964     940 
   Size:       2092     314     360    1013     245    1897

  Fragments arranged by size: 

                2092    1897    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   4102    4102 
   Size:       5921
 
NarI GG'CG_CC

Cuts at:   3352    3600    3352 
   Size:        248    5673
 
NdeI CA'TA_TG

Cuts at:    687    2220     687 
   Size:       1533    4388
 
NgoMIV G'CCGG_C

Cuts at:   4100    4100 
   Size:       5921
 
NsiI A_TGCA'T

Cuts at:    883    2540     883 
   Size:       1657    4264
 
NspI r_CATG'y

Cuts at:   2538    2557    2974    3474    4381    2538 
   Size:         19     417     500     907    4078

  Fragments arranged by size: 

                4078     907     500     417      19
 
PacI TTA_AT'TAA

Cuts at:   1079    1832    1079 
   Size:        753    5168
 
PciI A'CATG_T

Cuts at:   2553    4377    2553 
   Size:       1824    4097
 
PfoI T'CCnGG_A

Cuts at:    212    1140    2489     212 
   Size:        928    1349    3644

  Fragments arranged by size: 

                3644    1349     928
 
PmeI GTTT'AAAC

Cuts at:   2920    2920 
   Size:       5921
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5060    5092    5060 
   Size:         32    5889
 
PpuMI rG'GwC_Cy

Cuts at:    190     190 
   Size:       5921
 
PsiI TTA'TAA

Cuts at:    503     503 
   Size:       5921
 
PspXI vC'TCGA_Gb

Cuts at:   1709    1709 
   Size:       5921
 
PstI C_TGCA'G

Cuts at:   2652    2968    3653    2652 
   Size:        316     685    4920

  Fragments arranged by size: 

                4920     685     316
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5921
 
PvuII CAG'CTG

Cuts at:    940    3346    3706     940 
   Size:       2406     360    3155

  Fragments arranged by size: 

                3155    2406     360
 
RsrII CG'GwC_CG

Cuts at:   4116    4116 
   Size:       5921
 
SacI G_AGCT'C

Cuts at:   2935    2935 
   Size:       5921
 
SalI G'TCGA_C

Cuts at:   2958    2958 
   Size:       5921
 
SanDI GG'GwC_CC

Cuts at:    190     190 
   Size:       5921
 
SapI GCTCTTCn'nnn_

Cuts at:   3944    4154    3944 
   Size:        210    5711
 
SbfI CC_TGCA'GG

Cuts at:   2968    2968 
   Size:       5921
 
ScaI AGT'ACT

Cuts at:   2280    5438    2280 
   Size:       3158    2763
 
SfcI C'TryA_G

Cuts at:     33    1092    1733    2648    2964    3649    4642    4833      33 
   Size:       1059     641     915     316     685     993     191    1121

  Fragments arranged by size: 

                1121    1059     993     915     685     641     316     191
            
 
SfoI GGC'GCC

Cuts at:   3353    3601    3353 
   Size:        248    5673
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5921
 
SmaI CCC'GGG

Cuts at:   2943    2943 
   Size:       5921
 
SmlI C'TyrA_G

Cuts at:    347    1709    2568    2854    3076    3991    4483    4745     347 
   Size:       1362     859     286     222     915     492     262    1523

  Fragments arranged by size: 

                1523    1362     915     859     492     286     262     222
            
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5921
 
SphI G_CATG'C

Cuts at:   2974    3474    2974 
   Size:        500    5421
 
SspI AAT'ATT

Cuts at:   2236    2236 
   Size:       5921
 
StuI AGG'CCT

Cuts at:    117     117 
   Size:       5921
 
StyI C'CwwG_G

Cuts at:   1388    1463    2690    3055    3409    1388 
   Size:         75    1227     365     354    3900

  Fragments arranged by size: 

                3900    1227     365     354      75
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    228    3504    3637    4096    4305     228 
   Size:       3276     133     459     209    1844

  Fragments arranged by size: 

                3276    1844     459     209     133
 
TatI w'GTAC_w

Cuts at:    863    2278    2864    5436     863 
   Size:       1415     586    2572    1348

  Fragments arranged by size: 

                2572    1415    1348     586
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5438    5470    5438 
   Size:         32    5889
 
Tth111I GACn'n_nGTC

Cuts at:   1336    3718    1336 
   Size:       2382    3539
 
XbaI T'CTAG_A

Cuts at:   2952    2952 
   Size:       5921
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3416    3416 
   Size:       5921
 
XhoI C'TCGA_G

Cuts at:   1709    1709 
   Size:       5921
 
XmaI C'CCGG_G

Cuts at:   2941    2941 
   Size:       5921
 
XmnI GAAnn'nnTTC

Cuts at:    838     971    1232    1925    2876    2894     838 
   Size:        133     261     693     951      18    3865

  Fragments arranged by size: 

                3865     951     693     261     133      18
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI     AloI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NsiI     NspI     PacI     PciI     PfoI     PmeI     PpiI
    PpuMI     PsiI    PspXI     PstI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AleI     AlfI     ApaI     AscI
    AvrII     BaeI     BglI     BmtI    BseRI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII    BstXI     BtgI     ClaI   DraIII     FseI
    FspAI  HindIII     NcoI     NheI     NotI     NruI     PasI    PflMI
     PmlI    PshAI   PspOMI     PsrI    SacII    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SwaI     ZraI
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