Restriction map for pF1KB9985
 (Linear) (Six-base) MAPSORT of: KIBB9985.seq  Check: 1968  from: 1  to: 1716



KIBB9985.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:09  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1303    1716 
   Size:       1303     413
 
AatII G_ACGT'C

Cuts at:      0    1193    1716 
   Size:       1193     523
 
AccI GT'mk_AC

Cuts at:      0     635    1716 
   Size:        635    1081
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     909    1716 
   Size:        909     807
 
AflIII A'CryG_T

Cuts at:      0     902    1716 
   Size:        902     814
 
AleI CACnn'nnGTG

Cuts at:      0     907    1716 
   Size:        907     809
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1057    1716 
   Size:       1057     659
 
ApoI r'AATT_y

Cuts at:      0     513     744    1699    1716 
   Size:        513     231     955      17

  Fragments arranged by size: 

                 955     513     231      17
 
AvaI C'yCGr_G

Cuts at:      0    1659    1716 
   Size:       1659      57
 
BamHI G'GATC_C

Cuts at:      0    1677    1716 
   Size:       1677      39
 
BanI G'GyrC_C

Cuts at:      0     439    1203    1716 
   Size:        439     764     513

  Fragments arranged by size: 

                 764     513     439
 
BanII G_rGCy'C

Cuts at:      0     274    1716 
   Size:        274    1442
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     134     456     519     648    1716 
   Size:        134     322      63     129    1068

  Fragments arranged by size: 

                1068     322     134     129      63
 
BglII A'GATC_T

Cuts at:      0     771    1716 
   Size:        771     945
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     172    1716 
   Size:        172    1544
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1096    1716 
   Size:       1096     620
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1457    1716 
   Size:       1457     259
 
BsaAI yAC'GTr

Cuts at:      0     690     905    1716 
   Size:        690     215     811

  Fragments arranged by size: 

                 811     690     215
 
BsaHI Gr'CG_yC

Cuts at:      0    1190    1716 
   Size:       1190     526
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     215     245    1716 
   Size:        215      30    1471

  Fragments arranged by size: 

                1471     215      30
 
BseYI C'CCAG_C

Cuts at:      0      84    1467    1716 
   Size:         84    1383     249

  Fragments arranged by size: 

                1383     249      84
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     253    1716 
   Size:        253    1463
 
BsiEI CG_ry'CG

Cuts at:      0     741    1716 
   Size:        741     975
 
BsiHKAI G_wGCw'C

Cuts at:      0     122     914    1441    1716 
   Size:        122     792     527     275

  Fragments arranged by size: 

                 792     527     275     122
 
BsmI GAATG_Cn'

Cuts at:      0    1256    1716 
   Size:       1256     460
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1356    1716 
   Size:       1356     360
 
Bsp1286I G_dGCh'C

Cuts at:      0     122     274     914    1441    1716 
   Size:        122     152     640     527     275

  Fragments arranged by size: 

                 640     527     275     152     122
 
BspHI T'CATG_A

Cuts at:      0      75     801    1716 
   Size:         75     726     915

  Fragments arranged by size: 

                 915     726      75
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1065    1252    1303    1716 
   Size:       1065     187      51     413

  Fragments arranged by size: 

                1065     413     187      51
 
BsrDI GCAATG_nn'

Cuts at:      0      96    1716 
   Size:         96    1620
 
BsrFI r'CCGG_y

Cuts at:      0     442    1200    1716 
   Size:        442     758     516

  Fragments arranged by size: 

                 758     516     442
 
BsrGI T'GTAC_A

Cuts at:      0    1553    1716 
   Size:       1553     163
 
BssSI C'ACGA_G

Cuts at:      0     265    1434    1716 
   Size:        265    1169     282

  Fragments arranged by size: 

                1169     282     265
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     538    1716 
   Size:        538    1178
 
BstYI r'GATC_y

Cuts at:      0     771    1677    1716 
   Size:        771     906      39

  Fragments arranged by size: 

                 906     771      39
 
BtgI C'CryG_G

Cuts at:      0    1207    1262    1716 
   Size:       1207      55     454

  Fragments arranged by size: 

                1207     454      55
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     651    1716 
   Size:        651    1065
 
DraI TTT'AAA

Cuts at:      0    1704    1716 
   Size:       1704      12
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     411    1716 
   Size:        411    1305
 
EaeI y'GGCC_r

Cuts at:      0     444    1122    1520    1716 
   Size:        444     678     398     196

  Fragments arranged by size: 

                 678     444     398     196
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     959    1716 
   Size:        959     757
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     909    1096    1716 
   Size:        909     187     620

  Fragments arranged by size: 

                 909     620     187
 
EcoO109I rG'GnC_Cy

Cuts at:      0     961    1054    1716 
   Size:        961      93     662

  Fragments arranged by size: 

                 961     662      93
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     867    1716 
   Size:        867     849
 
HaeII r_GCGC'y

Cuts at:      0    1499    1716 
   Size:       1499     217
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      17      49     789     821    1456    1488    1716 
   Size:         17      32     740      32     635      32     228

  Fragments arranged by size: 

                 740     635     228      32      32      32      17
 
HincII GTy'rAC

Cuts at:      0    1170    1716 
   Size:       1170     546
 
HpaI GTT'AAC

Cuts at:      0    1170    1716 
   Size:       1170     546
 
MfeI C'AATT_G

Cuts at:      0    1145    1716 
   Size:       1145     571
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     776    1716 
   Size:        776     940
 
MscI TGG'CCA

Cuts at:      0    1124    1522    1716 
   Size:       1124     398     194

  Fragments arranged by size: 

                1124     398     194
 
MslI CAynn'nnrTG

Cuts at:      0     625     905     907    1180    1716 
   Size:        625     280       2     273     536

  Fragments arranged by size: 

                 625     536     280     273       2
 
NcoI C'CATG_G

Cuts at:      0    1207    1716 
   Size:       1207     509
 
NdeI CA'TA_TG

Cuts at:      0     657    1716 
   Size:        657    1059
 
PasI CC'CwG_GG

Cuts at:      0    1407    1716 
   Size:       1407     309
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     558    1716 
   Size:        558    1158
 
PfoI T'CCnGG_A

Cuts at:      0     214    1716 
   Size:        214    1502
 
PpuMI rG'GwC_Cy

Cuts at:      0     961    1054    1716 
   Size:        961      93     662

  Fragments arranged by size: 

                 961     662      93
 
PshAI GACnn'nnGTC

Cuts at:      0    1189    1716 
   Size:       1189     527
 
PstI C_TGCA'G

Cuts at:      0     361     954    1324    1716 
   Size:        361     593     370     392

  Fragments arranged by size: 

                 593     392     370     361
 
PvuI CG_AT'CG

Cuts at:      0     741    1716 
   Size:        741     975
 
SfcI C'TryA_G

Cuts at:      0     357     636     950    1320    1716 
   Size:        357     279     314     370     396

  Fragments arranged by size: 

                 396     370     357     314     279
 
SmaI CCC'GGG

Cuts at:      0    1661    1716 
   Size:       1661      55
 
StuI AGG'CCT

Cuts at:      0    1619    1716 
   Size:       1619      97
 
StyI C'CwwG_G

Cuts at:      0    1207    1248    1716 
   Size:       1207      41     468

  Fragments arranged by size: 

                1207     468      41
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1002    1321    1716 
   Size:       1002     319     395

  Fragments arranged by size: 

                1002     395     319
 
TatI w'GTAC_w

Cuts at:      0    1553    1716 
   Size:       1553     163
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     215     247    1330    1362    1377    1409    1716 
   Size:        215      32    1083      32      15      32     307

  Fragments arranged by size: 

                1083     307     215      32      32      32      15
 
Tth111I GACn'n_nGTC

Cuts at:      0     965    1234    1716 
   Size:        965     269     482

  Fragments arranged by size: 

                 965     482     269
 
XmaI C'CCGG_G

Cuts at:      0    1659    1716 
   Size:       1659      57
 
ZraI GAC'GTC

Cuts at:      0    1191    1716 
   Size:       1191     525
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AleI    AlwNI     ApoI
     AvaI    BamHI     BanI    BanII     BbsI    BglII     BmrI     BpmI
     BsaI    BsaAI    BsaHI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI
    BssSI    BstXI    BstYI     BtgI    BtgZI     DraI     DrdI     EaeI
     EciI  Eco57MI EcoO109I     FalI    HaeII    Hin4I   HincII     HpaI
     MfeI     MmeI     MscI     MslI     NcoI     NdeI     PasI    PflMI
     PfoI    PpuMI    PshAI     PstI     PvuI     SfcI     SmaI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AlfI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaBI    BsaWI    BseRI
    BsiWI    BspEI    BsrBI   BssHII   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI   DraIII     EagI     EarI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     KasI
     KpnI     MluI   MspA1I     NaeI     NarI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI
     PsiI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     XbaI     XcmI     XhoI     XmnI
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