Restriction map for pF1KE3637
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
Alw44I      ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AviII       
AvrII       BbeI        BbrPI       BciVI       BclI        
BfaI        BfrI        BglI        BlnI        BlpI        
Bpu1102I    BsaBI       BsaHI       BsaI        BseAI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DrdI        DsaI        EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        Hin1I       HincII      HindII      HpaI        
KasI        KpnI        KspI        MaeI        MamI        
MfeI        MluI        MroI        MseI        MslI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tru9I       Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccII         186  CGCG                 CG^CG
AhdI          106  GACAAGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         539  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspEI         106  GACAAGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          569  TGGCCA               TGG^CCA
BamHI         119  GGATCC               G^GATCC
BanI          783  GGCACC               G^G[CT][GA]CC
BbsI           57  GAAGAC               GAAGAC(2/6)
BbvCI         631  GCTGAGG              CCTCAGC(-5/-2)
BcgI          238  CGAAACCGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1451  AGATCT               A^GATCT
BpuAI          57  GAAGAC               GAAGAC(2/6)
BsaAI         988  TACGTG               [CT]AC^GT[GA]
BsaWI         777  ACCGGA               [AT]^CCGG[AT]
BseRI         483  CTCCTC               GAGGAG(10/8)
BsrBI         619  GAGCGG               CCGCTC(-3/-3)
BsrDI        1069  GCAATG               GCAATG(2/0)
BstUI         186  CGCG                 CG^CG
BstXI         560  CCAACAACTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII        814  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          309  CGGCCG               C^GGCCG
Eam1105I      106  GACAAGTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1513  CTCTTC               CTCTTC(1/4)
EclXI         309  CGGCCG               C^GGCCG
Eco52I        309  CGGCCG               C^GGCCG
FauI          621  GCGGG                CCCGC(4/6)
HgaI          827  GCGTC                GACGC(5/10)
HindIII      1411  AAGCTT               A^AGCTT
Ksp632I      1513  CTCTTC               CTCTTC(1/4)
MaeII         989  ACGT                 A^CGT
MluNI         569  TGGCCA               TGG^CCA
MscI          569  TGGCCA               TGG^CCA
MvnI          186  CGCG                 CG^CG
PflMI        1431  CCAGCATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         869  AGGACCT              [GA]G^G[AT]CC[CT]
SapI         1512  GCTCTTC              GCTCTTC(1/4)
SexAI         815  ACCTGGT              A^CC[AT]GGT
SmaI          902  CCCGGG               CCC^GGG
SphI          342  GCATGC               GCATG^C
TaqI          224  TCGA                 T^CGA
Tsp509I        75  AATT                 ^AATT
TthHB8I       224  TCGA                 T^CGA
Van91I       1431  CCAGCATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          980  CCATTCAGTACGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         902  CCCGGG               C^CCGGG
XmaI          902  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AfaI          987  GTAC                 GT^AC
             1329  GTAC                
AocI          499  CCTGAGG              CC^T[ACGT]AGG
              536  CCTCAGG             
Aor51HI       176  AGCGCT               AGC^GCT
              884  AGCGCT              
AvaI          290  CCCGAG               C^[CT]CG[GA]G
              902  CCCGGG              
AvaII         870  GGACC                G^G[AT]CC
             1479  GGTCC               
BsiEI         225  CGACCG               CG[GA][CT]^CG
              309  CGGCCG              
BsmAI          18  GAGAC                GTCTC(1/5)
              847  GAGAC               
BsoBI         290  CCCGAG               C^[CT]CG[GA]G
              902  CCCGGG              
BspMI         494  ACCTGC               ACCTGC(4/8)
              872  ACCTGC              
BstAPI        784  GCACCCTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              813  GCACCTGGTGC         
BstYI         119  GGATCC               [GA]^GATC[CT]
             1451  AGATCT              
Bsu36I        499  CCTGAGG              CC^T[ACGT]AGG
              536  CCTCAGG             
Eco47III      176  AGCGCT               AGC^GCT
              884  AGCGCT              
Eco81I        499  CCTGAGG              CC^T[ACGT]AGG
              536  CCTCAGG             
HinfI        1368  GACTC                G^A[ACGT]TC
             1531  GAGTC               
MflI          119  GGATCC               [GA]^GATC[CT]
             1451  AGATCT              
PleI         1368  GACTC                GAGTC(4/5)
             1531  GAGTC               
RsaI          987  GTAC                 GT^AC
             1329  GTAC                
Tsp45I        913  GTGAC                ^GT[GC]AC
             1533  GTCAC               
XhoII         119  GGATCC               [GA]^GATC[CT]
             1451  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AciI        AluI        AlwI        ApaI        AspHI       
BanII       BbvI        BcnI        BmyI        BpmI        
BsaJI       BsgI        BsiHKAI     BsiYI       BslI        
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoNI       EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HpaII       
HphI        ItaI        MaeIII      MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PstI        PvuII       SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TseI        
TspRI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com