Restriction map for pF1KB6512
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspHI       AspI        AvaI        AviII       
AvrII       BalI        BanI        BbeI        BbrPI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsoBI       BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DrdI        DsaI        EaeI        EagI        EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        KpnI        
Ksp632I     KspI        MaeII       MamI        MfeI        
MluI        MluNI       MroI        MscI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StyI        
SwaI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AciI          881  CCGC                 CCGC(-3/-1)
AvaII         322  GGTCC                G^G[AT]CC
BbsI          950  GAAGAC               GAAGAC(2/6)
BbvI         1110  GCAGC                GCAGC(8/12)
BcnI          917  CCCGG                CC^[GC]GG
BpuAI         950  GAAGAC               GAAGAC(2/6)
BsaJI         797  CCCTGG               C^C[ACGT][ACGT]GG
BseRI         739  GAGGAG               GAGGAG(10/8)
BsiYI         328  CCAGGTTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          328  CCAGGTTGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          241  GCATTC               GAATGC(1/-1)
Bsp1407I      416  TGTACA               T^GTACA
BsrGI         416  TGTACA               T^GTACA
BstAPI        436  GCATTTAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        716  CATCC                GGATG(2/0)
DraII         172  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DraIII        904  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I      172  AGGGCCT              [GA]G^G[ACGT]CC[CT]
FokI          716  CATCC                GGATG(9/13)
HapII         918  CCGG                 C^CGG
HpaII         918  CCGG                 C^CGG
MspI          918  CCGG                 C^CGG
NciI          917  CCCGG                CC^[GC]GG
PflMI         328  CCAGGTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfcI         1120  CTACAG               C^T[GA][CT]AG
SspBI         416  TGTACA               T^GTACA
StuI         1020  AGGCCT               AGG^CCT
TaqI          138  TCGA                 T^CGA
TseI         1110  GCAGC                G^C[AT]GC
TthHB8I       138  TCGA                 T^CGA
Van91I        328  CCAGGTTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          968  CCAAGTCCCCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI           59  GTAGAC               GT^[AC][GT]AC
             1000  GTCTAC              
AfaI          417  GTAC                 GT^AC
              933  GTAC                
AhdI          424  GACACAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              814  GACCTGGTGTC         
AlwNI         171  CAGGGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              428  CAGAGTCTG           
AspEI         424  GACACAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              814  GACCTGGTGTC         
BamHI         301  GGATCC               G^GATCC
              862  GGATCC              
BanII         794  GAGCCC               G[GA]GC[CT]^C
             1076  GGGCTC              
BbvCI        1096  CCTCAGC              CCTCAGC(-5/-2)
              736  GCTGAGG             
BfaI          886  CTAG                 C^TAG
             1114  CTAG                
BsmAI        1036  GAGAC                GTCTC(1/5)
              822  GTCTC               
BsmFI         840  GTCCC                GGGAC(10/14)
              972  GTCCC               
BstXI         383  CCACCAACATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              783  CCATCTGAGTGG        
Cfr13I        173  GGGCC                G^G[ACGT]CC
              322  GGTCC               
Eam1105I      424  GACACAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              814  GACCTGGTGTC         
Fnu4HI        880  GCCGC                GC^[ACGT]GC
             1110  GCAGC               
HaeIII        174  GGCC                 GG^CC
             1021  GGCC                
ItaI          880  GCCGC                GC^[ACGT]GC
             1110  GCAGC               
MaeI          886  CTAG                 C^TAG
             1114  CTAG                
MseI          491  TTAA                 T^TAA
              611  TTAA                
MspA1I        827  CAGCTG               C[AC]G^C[GT]G
              867  CAGCTG              
PvuII         827  CAGCTG               CAG^CTG
              867  CAGCTG              
RsaI          417  GTAC                 GT^AC
              933  GTAC                
Sau96I        173  GGGCC                G^G[ACGT]CC
              322  GGTCC               
SexAI         327  ACCAGGT              A^CC[AT]GGT
              815  ACCTGGT             
Tru9I         491  TTAA                 T^TAA
              611  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AluI        AlwI        BmyI        BpmI        Bsp1286I    
BsrI        BssKI       BstNI       BstYI       DdeI        
DpnII       EcoRII      HinfI       HphI        MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        Sau3AI      ScrFI       SfaNI       TfiI        
Tsp45I      Tsp509I     TspRI       XhoII       
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