Restriction map for pF1KB6512
 (Linear) (Six-base) MAPSORT of: KIBB6512.seq  Check: 5996  from: 1  to: 1152



KIBB6512.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AccI GT'mk_AC

Cuts at:      0      60    1001    1152 
   Size:         60     941     151

  Fragments arranged by size: 

                 941     151      60
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     518     869    1152 
   Size:        518     351     283

  Fragments arranged by size: 

                 518     351     283
 
AhdI GACnn_n'nnGTC

Cuts at:      0     429     819    1152 
   Size:        429     390     333

  Fragments arranged by size: 

                 429     390     333
 
AlwNI CAG_nnn'CTG

Cuts at:      0     176     433    1152 
   Size:        176     257     719

  Fragments arranged by size: 

                 719     257     176
 
BamHI G'GATC_C

Cuts at:      0     301     862    1152 
   Size:        301     561     290

  Fragments arranged by size: 

                 561     301     290
 
BanII G_rGCy'C

Cuts at:      0     798    1080    1152 
   Size:        798     282      72

  Fragments arranged by size: 

                 798     282      72
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     957    1152 
   Size:        957     195
 
BbvCI CC'TCA_GC

Cuts at:      0     737    1097    1152 
   Size:        737     360      55

  Fragments arranged by size: 

                 737     360      55
 
Bme1580I G_kGCm'C

Cuts at:      0     183    1152 
   Size:        183     969
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     748    1083    1152 
   Size:        748     335      69

  Fragments arranged by size: 

                 748     335      69
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     735    1152 
   Size:        735     417
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     809     913     918    1064    1152 
   Size:        127     682     104       5     146      88

  Fragments arranged by size: 

                 682     146     127     104      88       5
 
Bpu10I CC'TnA_GC

Cuts at:      0     737    1097    1106    1152 
   Size:        737     360       9      46

  Fragments arranged by size: 

                 737     360      46       9
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     398     428     891     921    1152 
   Size:        398      30     463      30     231

  Fragments arranged by size: 

                 463     398     231      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     754    1152 
   Size:        754     398
 
BseYI C'CCAG_C

Cuts at:      0    1139    1152 
   Size:       1139      13
 
BsmI GAATG_Cn'

Cuts at:      0     241    1152 
   Size:        241     911
 
Bsp1286I G_dGCh'C

Cuts at:      0     183     798    1080    1152 
   Size:        183     615     282      72

  Fragments arranged by size: 

                 615     282     183      72
 
BsrGI T'GTAC_A

Cuts at:      0     416    1152 
   Size:        416     736
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     442    1152 
   Size:        442     710
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     390     790    1152 
   Size:        390     400     362

  Fragments arranged by size: 

                 400     390     362
 
BstYI r'GATC_y

Cuts at:      0      68     301     862    1152 
   Size:         68     233     561     290

  Fragments arranged by size: 

                 561     290     233      68
 
DraIII CAC_nnn'GTG

Cuts at:      0     909    1152 
   Size:        909     243
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     518     809     869     913     918    1064    1152 
   Size:        127     391     291      60      44       5     146      88

  Fragments arranged by size: 

                 391     291     146     127      88      60      44       5
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     173    1152 
   Size:        173     979
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     550    1152 
   Size:        550     602
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      48      53      80      85     306     338     415     447 
   Size:         48       5      27       5     221      32      77      32

Cuts at:    447     805     823     837     855    1152 
   Size:        358      18      14      18     297

  Fragments arranged by size: 

                 358     297     221      77      48      32      32      27
                  18      18      14       5       5
 
MslI CAynn'nnrTG

Cuts at:      0     287     388     788    1152 
   Size:        287     101     400     364

  Fragments arranged by size: 

                 400     364     287     101
 
MspA1I CmG'CkG

Cuts at:      0     829     869    1152 
   Size:        829      40     283

  Fragments arranged by size: 

                 829     283      40
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     334    1152 
   Size:        334     818
 
PvuII CAG'CTG

Cuts at:      0     829     869    1152 
   Size:        829      40     283

  Fragments arranged by size: 

                 829     283      40
 
SexAI A'CCwGG_T

Cuts at:      0     327     815    1152 
   Size:        327     488     337

  Fragments arranged by size: 

                 488     337     327
 
SfcI C'TryA_G

Cuts at:      0    1120    1152 
   Size:       1120      32
 
StuI AGG'CCT

Cuts at:      0    1022    1152 
   Size:       1022     130
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1072    1152 
   Size:       1072      80
 
TatI w'GTAC_w

Cuts at:      0     416    1152 
   Size:        416     736
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     398     430     891     923    1152 
   Size:        398      32     461      32     229

  Fragments arranged by size: 

                 461     398     229      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     975    1152 
   Size:        975     177
 

 Enzymes that do cut:

     AccI     AcuI     AhdI    AlwNI    BamHI    BanII     BbsI    BbvCI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BsaXI    BseRI    BseYI
     BsmI Bsp1286I    BsrGI   BstAPI    BstXI    BstYI   DraIII  Eco57MI
 EcoO109I     FalI    Hin4I     MslI   MspA1I    PflMI    PvuII    SexAI
     SfcI     StuI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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