Restriction map for pF1KE1351
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       AccI        AclI        AcyI        AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        AspEI       
AspHI       AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsaI        BsiHKAI     BsiWI       BsmBI       
BspDI       BspHI       BspLU11I    BsrDI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     CelII       CfoI        
ClaI        CpoI        DraI        DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        KasI        KspI        
MamI        MfeI        MluI        MunI        NarI        
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspI        StuI        SwaI        TaqI        
Tth111I     TthHB8I     XbaI        XhoI        


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# ONE CUT ENZYMES (   46 enzymes) #
###################################

Acc65I        462  GGTACC               G^GTACC
AccIII        575  TCCGGA               T^CCGGA
AflII         858  CTTAAG               C^TTAAG
AlwI         1579  GATCC                GGATC(4/5)
AocI         1480  CCTGAGG              CC^T[ACGT]AGG
Asp718I       462  GGTACC               G^GTACC
AvaI         1393  CCCGGG               C^[CT]CG[GA]G
BfrI          858  CTTAAG               C^TTAAG
BglI         1135  GCCAAGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI         1274  CTCCAG               CTGGAG(16/14)
BsaAI        1775  TACGTG               [CT]AC^GT[GA]
BsaWI         575  TCCGGA               [AT]^CCGG[AT]
BseAI         575  TCCGGA               T^CCGGA
BsmI          507  GAATGC               GAATGC(1/-1)
BsoBI        1393  CCCGGG               C^[CT]CG[GA]G
Bsp1407I     1439  TGTACA               T^GTACA
BspEI         575  TCCGGA               T^CCGGA
BspMI         171  ACCTGC               ACCTGC(4/8)
BsrBI        1890  CCGCTC               CCGCTC(-3/-3)
BsrGI        1439  TGTACA               T^GTACA
BstYI        1578  AGATCC               [GA]^GATC[CT]
Bsu36I       1480  CCTGAGG              CC^T[ACGT]AGG
DrdI         1288  GACATCATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1480  CCTGAGG              CC^T[ACGT]AGG
EcoNI         375  CCTTAATCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII       253  AAGCTT               A^AGCTT
KpnI          462  GGTACC               GGTAC^C
MflI         1578  AGATCC               [GA]^GATC[CT]
MroI          575  TCCGGA               T^CCGGA
MslI         1791  CATCTGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         45  CAGCGG               C[AC]G^C[GT]G
NaeI         1654  GCCGGC               GCC^GGC
NcoI          262  CCATGG               C^CATGG
NdeI          968  CATATG               CA^TATG
NgoMIV       1654  GCCGGC               G^CCGGC
NspI          125  GCATGC               [GA]CATG^[CT]
PshAI        1239  GACCGTAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI          922  CTGCAG               CTGCA^G
ScaI         1638  AGTACT               AGT^ACT
SmaI         1393  CCCGGG               CCC^GGG
SphI          125  GCATGC               GCATG^C
SspBI        1439  TGTACA               T^GTACA
XcmI          420  CCACATCCACACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII        1578  AGATCC               [GA]^GATC[CT]
XmaCI        1393  CCCGGG               C^CCGGG
XmaI         1393  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccII          37  CGCG                 CG^CG
              143  CGCG                
AcsI          333  GAATTT               [GA]^AATT[CT]
             1615  GAATTT              
ApoI          333  GAATTT               [GA]^AATT[CT]
             1615  GAATTT              
Asp700I       251  GAAAGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              822  GAAGGAGTTC          
BciVI        1062  GGATAC               GTATCC(6/5)
             1697  GTATCC              
BsiEI          14  CGGTCG               CG[GA][CT]^CG
             1146  CGACCG              
BsrFI        1148  ACCGGC               [GA]^CCGG[CT]
             1654  GCCGGC              
BstUI          37  CGCG                 CG^CG
              143  CGCG                
Cfr10I       1148  ACCGGC               [GA]^CCGG[CT]
             1654  GCCGGC              
DsaI          262  CCATGG               C^C[GA][CT]GG
             1190  CCGTGG              
HgaI         1197  GACGC                GACGC(5/10)
               65  GCGTC               
MaeII         553  ACGT                 A^CGT
             1776  ACGT                
MvnI           37  CGCG                 CG^CG
              143  CGCG                
PflMI         446  CCATGTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              843  CCAATACCTGG         
PleI          277  GACTC                GAGTC(4/5)
             1710  GACTC               
SmlI          772  CTCAAG               C^T[CT][GA]AG
              858  CTTAAG              
Van91I        446  CCATGTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              843  CCAATACCTGG         
XmnI          251  GAAAGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              822  GAAGGAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpuAI       BsaJI       
BseRI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MseI        MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PpuMI       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
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