Restriction map for pF1KE2271
 (Circular) (Six-base) MAPSORT of: pF1KE2271.seq  Check: 9749  from: 1  to: 4487



pF1KE2271  4487 bp

 With 182 enzymes: SgfI * 

                        November 2, 2010 17:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    512     512 
   Size:       4487
 
AccI GT'mk_AC

Cuts at:   1525    1525 
   Size:       4487
 
Acc65I G'GTAC_C

Cuts at:   1503    1503 
   Size:       4487
 
AclI AA'CG_TT

Cuts at:   4327    4327 
   Size:       4487
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    493     895    2312    2744    3491    3759     493 
   Size:        402    1417     432     747     268    1221

  Fragments arranged by size: 

                1417    1221     747     432     402     268
 
AflII C'TTAA_G

Cuts at:    403     403 
   Size:       4487
 
AflIII A'CryG_T

Cuts at:   1468    1839    2943    3920    1468 
   Size:        371    1104     977    2035

  Fragments arranged by size: 

                2035    1104     977     371
 
AgeI A'CCGG_T

Cuts at:   1454    2875    1454 
   Size:       1421    3066
 
AleI CACnn'nnGTG

Cuts at:   1285    1285 
   Size:       4487
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    682     682 
   Size:       4487
 
AlwNI CAG_nnn'CTG

Cuts at:    522    1218    1242    1792    3359     522 
   Size:        696      24     550    1567    1650

  Fragments arranged by size: 

                1650    1567     696     550      24
 
ApaLI G'TGCA_C

Cuts at:   3257    3257 
   Size:       4487
 
ApoI r'AATT_y

Cuts at:   1080    1491    3840    4096    1080 
   Size:        411    2349     256    1471

  Fragments arranged by size: 

                2349    1471     411     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4487
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4487
 
AvaI C'yCGr_G

Cuts at:   1230    1507    1230 
   Size:        277    4210
 
BamHI G'GATC_C

Cuts at:   1512    1512 
   Size:       4487
 
BanI G'GyrC_C

Cuts at:    646    1370    1376    1385    1503    1917    2165    2200     646 
   Size:        724       6       9     118     414     248      35    2933

  Fragments arranged by size: 

                2933     724     414     248     118      35       9       6
            
 
BanII G_rGCy'C

Cuts at:    307     544     635    1501    2531     307 
   Size:        237      91     866    1030    2263

  Fragments arranged by size: 

                2263    1030     866     237      91
 
BbeI G_GCGC'C

Cuts at:   1389    1921    2169    1389 
   Size:        532     248    3707

  Fragments arranged by size: 

                3707     532     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    419     613    1441    1471     419 
   Size:        194     828      30    3435

  Fragments arranged by size: 

                3435     828     194      30
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1142    1176    1142 
   Size:         34    4453
 
BciVI GTATCCnnnnn_n'

Cuts at:    204    2385    3146    4487     204 
   Size:       2181     761    1341     204

  Fragments arranged by size: 

                2181    1341     761     204
 
BclI T'GATC_A

Cuts at:    389    2007     389 
   Size:       1618    2869
 
BglII A'GATC_T

Cuts at:   2002    4039    2002 
   Size:       2037    2450
 
BlpI GC'TnA_GC

Cuts at:   1126    1599    1126 
   Size:        473    4014
 
Bme1580I G_kGCm'C

Cuts at:   1450    2112    2205    3261    1450 
   Size:        662      93    1056    2676

  Fragments arranged by size: 

                2676    1056     662      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1699    1780    2115    4259    1699 
   Size:         81     335    2144    1927

  Fragments arranged by size: 

                2144    1927     335      81
 
BmtI G_CTAG'C

Cuts at:    715     715 
   Size:       4487
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1082    2854    1082 
   Size:       1772    2715
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    619     649     714     907    1057    1159     619 
   Size:         30      65     193     150     102    3947

  Fragments arranged by size: 

                3947     193     150     102      65      30
 
Bpu10I CC'TnA_GC

Cuts at:    917    1115     917 
   Size:        198    4289
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    336    1663    2542    3034    3332     336 
   Size:       1327     879     492     298    1491

  Fragments arranged by size: 

                1491    1327     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1164    4191    1164 
   Size:       3027    1460
 
BsaAI yAC'GTr

Cuts at:   1840    1840 
   Size:       4487
 
BsaBI GATnn'nnATC

Cuts at:      6    2001    4038       6 
   Size:       1995    2037     455

  Fragments arranged by size: 

                2037    1995     455
 
BsaHI Gr'CG_yC

Cuts at:   1319    1386    1918    2166    2868    4364    1319 
   Size:         67     532     248     702    1496    1442

  Fragments arranged by size: 

                1496    1442     702     532     248      67
 
BsaWI w'CCGG_w

Cuts at:   1454    1676    2197    2875    3149    3296    3707    1454 
   Size:        222     521     678     274     147     411    2234

  Fragments arranged by size: 

                2234     678     521     411     274     222     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    143     173     143 
   Size:         30    4457
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    151     469     837    1411     151 
   Size:        318     368     574    3227

  Fragments arranged by size: 

                3227     574     368     318
 
BseYI C'CCAG_C

Cuts at:    165     195    1311    1912    2670    3247    3743     165 
   Size:         30    1116     601     758     577     496     909

  Fragments arranged by size: 

                1116     909     758     601     577     496      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1309    1309 
   Size:       4487
 
BsiEI CG_ry'CG

Cuts at:    104    2029    2075    3283     104 
   Size:       1925      46    1208    1308

  Fragments arranged by size: 

                1925    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    635    1501    2279    2469    3261     635 
   Size:        866     778     190     792    1861

  Fragments arranged by size: 

                1861     866     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    307     544     635    1450    1501    2112    2205    2279    2469 
   Size:        237      91     815      51     611      93      74     190

Cuts at:   2469    2531    3261     307 
   Size:         62     730    1533

  Fragments arranged by size: 

                1533     815     730     611     237     190      93      91
                  74      62      51
 
BspEI T'CCGG_A

Cuts at:   3707    3707 
   Size:       4487
 
BspHI T'CATG_A

Cuts at:   3663    3663 
   Size:       4487
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    512    1447    1537    1751    2053    2434     512 
   Size:        935      90     214     302     381    2565

  Fragments arranged by size: 

                2565     935     381     302     214      90
 
BsrBI CCG'CTC

Cuts at:     50     945    1398    2779    2833    4317    4482      50 
   Size:        895     453    1381      54    1484     165      55

  Fragments arranged by size: 

                1484    1381     895     453     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    574    2399     574 
   Size:       1825    2662
 
BsrFI r'CCGG_y

Cuts at:   1324    1373    1454    2485    2666    2875    1324 
   Size:         49      81    1031     181     209    2936

  Fragments arranged by size: 

                2936    1031     209     181      81      49
 
BsrGI T'GTAC_A

Cuts at:    229     229 
   Size:       4487
 
BssSI C'ACGA_G

Cuts at:   2758    3116    2758 
   Size:        358    4129
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1218    1864    1218 
   Size:        646    3841
 
BstBI TT'CG_AA

Cuts at:   2848    2848 
   Size:       4487
 
BstEII G'GTnAC_C

Cuts at:    903     903 
   Size:       4487
 
BstXI CCAn_nnnn'nTGG

Cuts at:    643     643 
   Size:       4487
 
BstYI r'GATC_y

Cuts at:      1    1512    1979    2002    2337    2583    3595    3681    3693 
   Size:       1511     467      23     335     246    1012      86      12

Cuts at:   3693    4039       1 
   Size:        346     449

  Fragments arranged by size: 

                1511    1012     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1196    1196 
   Size:       4487
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2437    2618    2781    2437 
   Size:        181     163    4143

  Fragments arranged by size: 

                4143     181     163
 
BtsI GCAGTG_nn'

Cuts at:    816    1219    1730     816 
   Size:        403     511    3573

  Fragments arranged by size: 

                3573     511     403
 
DraI TTT'AAA

Cuts at:   1486    1486 
   Size:       4487
 
DrdI GACnn_nn'nnGTC

Cuts at:   2193    3051    2193 
   Size:        858    3629
 
EaeI y'GGCC_r

Cuts at:   2072    2246    2637    2664    4009    2072 
   Size:        174     391      27    1345    2550

  Fragments arranged by size: 

                2550    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2072    2072 
   Size:       4487
 
EarI CTCTTCn'nnn_

Cuts at:    241    2510    2720     241 
   Size:       2269     210    2008

  Fragments arranged by size: 

                2269    2008     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    847    2695    3005    3151    4297     847 
   Size:       1848     310     146    1146    1037

  Fragments arranged by size: 

                1848    1146    1037     310     146
 
EcoICRI GAG'CTC

Cuts at:    633    1499     633 
   Size:        866    3621
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    493     619     649     714     895     907    1057    1159    2312 
   Size:        126      30      65     181      12     150     102    1153

Cuts at:   2312    2744    3491    3759     493 
   Size:        432     747     268    1221

  Fragments arranged by size: 

                1221    1153     747     432     268     181     150     126
                 102      65      30      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:    508     961     508 
   Size:        453    4034
 
EcoO109I rG'GnC_Cy

Cuts at:    912    1102    1239    1626     912 
   Size:        190     137     387    3773

  Fragments arranged by size: 

                3773     387     190     137
 
EcoRI G'AATT_C

Cuts at:   1491    1491 
   Size:       4487
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2506    2506 
   Size:       4487
 
FspI TGC'GCA

Cuts at:   2268    2268 
   Size:       4487
 
HaeII r_GCGC'y

Cuts at:    160    1389    1921    2169    3191    4170     160 
   Size:       1229     532     248    1022     979     477

  Fragments arranged by size: 

                1229    1022     979     532     477     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1387    1419    2329    2359    2361    2391    1387 
   Size:         32     910      30       2      30    3483

  Fragments arranged by size: 

                3483     910      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1526    1526 
   Size:       4487
 
KasI G'GCGC_C

Cuts at:   1385    1917    2165    1385 
   Size:        532     248    3707

  Fragments arranged by size: 

                3707     532     248
 
KpnI G_GTAC'C

Cuts at:   1507    1507 
   Size:       4487
 
MfeI C'AATT_G

Cuts at:    463     463 
   Size:       4487
 
MluI A'CGCG_T

Cuts at:   3920    3920 
   Size:       4487
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    299    1277    3158    3342     299 
   Size:        978    1881     184    1444

  Fragments arranged by size: 

                1881    1444     978     184
 
MscI TGG'CCA

Cuts at:   2248    2248 
   Size:       4487
 
MslI CAynn'nnrTG

Cuts at:    706    1285    2476    2603     706 
   Size:        579    1191     127    2590

  Fragments arranged by size: 

                2590    1191     579     127
 
MspA1I CmG'CkG

Cuts at:    627    1598    1912    2272    3285    3530     627 
   Size:        971     314     360    1013     245    1584

  Fragments arranged by size: 

                1584    1013     971     360     314     245
 
NaeI GCC'GGC

Cuts at:   1326    1375    2668    1326 
   Size:         49    1293    3145

  Fragments arranged by size: 

                3145    1293      49
 
NarI GG'CG_CC

Cuts at:   1386    1918    2166    1386 
   Size:        532     248    3707

  Fragments arranged by size: 

                3707     532     248
 
NgoMIV G'CCGG_C

Cuts at:   1324    1373    2666    1324 
   Size:         49    1293    3145

  Fragments arranged by size: 

                3145    1293      49
 
NheI G'CTAG_C

Cuts at:    711     711 
   Size:       4487
 
NspI r_CATG'y

Cuts at:    659    1472    1540    2040    2947     659 
   Size:        813      68     500     907    2199

  Fragments arranged by size: 

                2199     907     813     500      68
 
PciI A'CATG_T

Cuts at:   1468    2943    1468 
   Size:       1475    3012
 
PflMI CCAn_nnn'nTGG

Cuts at:    857     857 
   Size:       4487
 
PfoI T'CCnGG_A

Cuts at:    635     635 
   Size:       4487
 
PmeI GTTT'AAAC

Cuts at:   1486    1486 
   Size:       4487
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3626    3658    3626 
   Size:         32    4455
 
PpuMI rG'GwC_Cy

Cuts at:    912    1102    1239     912 
   Size:        190     137    4160

  Fragments arranged by size: 

                4160     190     137
 
PstI C_TGCA'G

Cuts at:   1534    2219    1534 
   Size:        685    3802
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4487
 
PvuII CAG'CTG

Cuts at:    627    1912    2272     627 
   Size:       1285     360    2842

  Fragments arranged by size: 

                2842    1285     360
 
RsrII CG'GwC_CG

Cuts at:   2682    2682 
   Size:       4487
 
SacI G_AGCT'C

Cuts at:    635    1501     635 
   Size:        866    3621
 
SalI G'TCGA_C

Cuts at:   1524    1524 
   Size:       4487
 
SanDI GG'GwC_CC

Cuts at:   1102    1102 
   Size:       4487
 
SapI GCTCTTCn'nnn_

Cuts at:    241    2510    2720     241 
   Size:       2269     210    2008

  Fragments arranged by size: 

                2269    2008     210
 
SbfI CC_TGCA'GG

Cuts at:   1534    1534 
   Size:       4487
 
ScaI AGT'ACT

Cuts at:   4004    4004 
   Size:       4487
 
SexAI A'CCwGG_T

Cuts at:   1046    1046 
   Size:       4487
 
SfcI C'TryA_G

Cuts at:     33    1530    2215    3208    3399      33 
   Size:       1497     685     993     191    1121

  Fragments arranged by size: 

                1497    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:   1387    1919    2167    1387 
   Size:        532     248    3707

  Fragments arranged by size: 

                3707     532     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4487
 
SmaI CCC'GGG

Cuts at:   1509    1509 
   Size:       4487
 
SmlI C'TyrA_G

Cuts at:    351     403    1642    2557    3049    3311     351 
   Size:         52    1239     915     492     262    1527

  Fragments arranged by size: 

                1527    1239     915     492     262      52
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4487
 
SphI G_CATG'C

Cuts at:   1540    2040    1540 
   Size:        500    3987
 
StuI AGG'CCT

Cuts at:    727     967     727 
   Size:        240    4247
 
StyI C'CwwG_G

Cuts at:    372     533     580    1621    1975     372 
   Size:        161      47    1041     354    2884

  Fragments arranged by size: 

                2884    1041     354     161      47
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    514    1173    2070    2203    2662    2871     514 
   Size:        659     897     133     459     209    2130

  Fragments arranged by size: 

                2130     897     659     459     209     133
 
TatI w'GTAC_w

Cuts at:    229    4002     229 
   Size:       3773     714
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    173     205    4004    4036     173 
   Size:         32    3799      32     624

  Fragments arranged by size: 

                3799     624      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2284    2284 
   Size:       4487
 
XbaI T'CTAG_A

Cuts at:   1518    1518 
   Size:       4487
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1982    1982 
   Size:       4487
 
XmaI C'CCGG_G

Cuts at:   1507    1507 
   Size:       4487
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI
    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI   Bsu36I    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NheI     NspI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI     AhdI     AloI     ApaI     AscI    AvrII     BaeI
    BbvCI    BfrBI     BglI    BmgBI    BsiWI     BsmI    BsmBI   BssHII
  BstZ17I     BtgI     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI
  HindIII     HpaI     NcoI     NdeI     NotI     NruI     NsiI     PacI
     PasI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI
     ZraI
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