Restriction map for pF1KE2271
 (Linear) (Six-base) MAPSORT of: KIEE2271.seq  Check: 3223  from: 1  to: 1374



KIEE2271.orf

 With 182 enzymes: SgfI * 

                        November 2, 2010 17:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     404    1374 
   Size:        404     970
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     787    1374 
   Size:        385     402     587

  Fragments arranged by size: 

                 587     402     385
 
AflII C'TTAA_G

Cuts at:      0     295    1374 
   Size:        295    1079
 
AflIII A'CryG_T

Cuts at:      0    1360    1374 
   Size:       1360      14
 
AgeI A'CCGG_T

Cuts at:      0    1346    1374 
   Size:       1346      28
 
AleI CACnn'nnGTG

Cuts at:      0    1177    1374 
   Size:       1177     197
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     574    1374 
   Size:        574     800
 
AlwNI CAG_nnn'CTG

Cuts at:      0     414    1110    1134    1374 
   Size:        414     696      24     240

  Fragments arranged by size: 

                 696     414     240      24
 
ApoI r'AATT_y

Cuts at:      0     972    1374 
   Size:        972     402
 
AvaI C'yCGr_G

Cuts at:      0    1122    1374 
   Size:       1122     252
 
BanI G'GyrC_C

Cuts at:      0     538    1262    1268    1277    1374 
   Size:        538     724       6       9      97

  Fragments arranged by size: 

                 724     538      97       9       6
 
BanII G_rGCy'C

Cuts at:      0     199     436     527    1374 
   Size:        199     237      91     847

  Fragments arranged by size: 

                 847     237     199      91
 
BbeI G_GCGC'C

Cuts at:      0    1281    1374 
   Size:       1281      93
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     311     505    1333    1363    1374 
   Size:        311     194     828      30      11

  Fragments arranged by size: 

                 828     311     194      30      11
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1034    1068    1374 
   Size:       1034      34     306

  Fragments arranged by size: 

                1034     306      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      96    1374 
   Size:         96    1278
 
BclI T'GATC_A

Cuts at:      0     281    1374 
   Size:        281    1093
 
BlpI GC'TnA_GC

Cuts at:      0    1018    1374 
   Size:       1018     356
 
Bme1580I G_kGCm'C

Cuts at:      0    1342    1374 
   Size:       1342      32
 
BmtI G_CTAG'C

Cuts at:      0     607    1374 
   Size:        607     767
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     974    1374 
   Size:        974     400
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     541     606     799     949    1051    1374 
   Size:        511      30      65     193     150     102     323

  Fragments arranged by size: 

                 511     323     193     150     102      65      30
 
Bpu10I CC'TnA_GC

Cuts at:      0     809    1007    1374 
   Size:        809     198     367

  Fragments arranged by size: 

                 809     367     198
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     228    1374 
   Size:        228    1146
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1056    1374 
   Size:       1056     318
 
BsaHI Gr'CG_yC

Cuts at:      0    1211    1278    1374 
   Size:       1211      67      96

  Fragments arranged by size: 

                1211      96      67
 
BsaWI w'CCGG_w

Cuts at:      0    1346    1374 
   Size:       1346      28
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      35      65    1374 
   Size:         35      30    1309

  Fragments arranged by size: 

                1309      35      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      43     361     729    1303    1374 
   Size:         43     318     368     574      71

  Fragments arranged by size: 

                 574     368     318      71      43
 
BseYI C'CCAG_C

Cuts at:      0      57      87    1203    1374 
   Size:         57      30    1116     171

  Fragments arranged by size: 

                1116     171      57      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1201    1374 
   Size:       1201     173
 
BsiHKAI G_wGCw'C

Cuts at:      0     527    1374 
   Size:        527     847
 
Bsp1286I G_dGCh'C

Cuts at:      0     199     436     527    1342    1374 
   Size:        199     237      91     815      32

  Fragments arranged by size: 

                 815     237     199      91      32
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     404    1339    1374 
   Size:        404     935      35

  Fragments arranged by size: 

                 935     404      35
 
BsrBI CCG'CTC

Cuts at:      0     837    1290    1374 
   Size:        837     453      84

  Fragments arranged by size: 

                 837     453      84
 
BsrDI GCAATG_nn'

Cuts at:      0     466    1374 
   Size:        466     908
 
BsrFI r'CCGG_y

Cuts at:      0    1216    1265    1346    1374 
   Size:       1216      49      81      28

  Fragments arranged by size: 

                1216      81      49      28
 
BsrGI T'GTAC_A

Cuts at:      0     121    1374 
   Size:        121    1253
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1110    1374 
   Size:       1110     264
 
BstEII G'GTnAC_C

Cuts at:      0     795    1374 
   Size:        795     579
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     535    1374 
   Size:        535     839
 
Bsu36I CC'TnA_GG

Cuts at:      0    1088    1374 
   Size:       1088     286
 
BtsI GCAGTG_nn'

Cuts at:      0     708    1111    1374 
   Size:        708     403     263

  Fragments arranged by size: 

                 708     403     263
 
EarI CTCTTCn'nnn_

Cuts at:      0     133    1374 
   Size:        133    1241
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     739    1374 
   Size:        739     635
 
EcoICRI GAG'CTC

Cuts at:      0     525    1374 
   Size:        525     849
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     511     541     606     787     799     949    1051 
   Size:        385     126      30      65     181      12     150     102

Cuts at:   1051    1374 
   Size:        323

  Fragments arranged by size: 

                 385     323     181     150     126     102      65      30
                  12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     400     853    1374 
   Size:        400     453     521

  Fragments arranged by size: 

                 521     453     400
 
EcoO109I rG'GnC_Cy

Cuts at:      0     804     994    1131    1374 
   Size:        804     190     137     243

  Fragments arranged by size: 

                 804     243     190     137
 
HaeII r_GCGC'y

Cuts at:      0      52    1281    1374 
   Size:         52    1229      93

  Fragments arranged by size: 

                1229      93      52
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1279    1311    1374 
   Size:       1279      32      63

  Fragments arranged by size: 

                1279      63      32
 
KasI G'GCGC_C

Cuts at:      0    1277    1374 
   Size:       1277      97
 
MfeI C'AATT_G

Cuts at:      0     355    1374 
   Size:        355    1019
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     191    1169    1374 
   Size:        191     978     205

  Fragments arranged by size: 

                 978     205     191
 
MslI CAynn'nnrTG

Cuts at:      0     598    1177    1374 
   Size:        598     579     197

  Fragments arranged by size: 

                 598     579     197
 
MspA1I CmG'CkG

Cuts at:      0     519    1374 
   Size:        519     855
 
NaeI GCC'GGC

Cuts at:      0    1218    1267    1374 
   Size:       1218      49     107

  Fragments arranged by size: 

                1218     107      49
 
NarI GG'CG_CC

Cuts at:      0    1278    1374 
   Size:       1278      96
 
NgoMIV G'CCGG_C

Cuts at:      0    1216    1265    1374 
   Size:       1216      49     109

  Fragments arranged by size: 

                1216     109      49
 
NheI G'CTAG_C

Cuts at:      0     603    1374 
   Size:        603     771
 
NspI r_CATG'y

Cuts at:      0     551    1364    1374 
   Size:        551     813      10

  Fragments arranged by size: 

                 813     551      10
 
PciI A'CATG_T

Cuts at:      0    1360    1374 
   Size:       1360      14
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     749    1374 
   Size:        749     625
 
PfoI T'CCnGG_A

Cuts at:      0     527    1374 
   Size:        527     847
 
PpuMI rG'GwC_Cy

Cuts at:      0     804     994    1131    1374 
   Size:        804     190     137     243

  Fragments arranged by size: 

                 804     243     190     137
 
PvuII CAG'CTG

Cuts at:      0     519    1374 
   Size:        519     855
 
SacI G_AGCT'C

Cuts at:      0     527    1374 
   Size:        527     847
 
SanDI GG'GwC_CC

Cuts at:      0     994    1374 
   Size:        994     380
 
SapI GCTCTTCn'nnn_

Cuts at:      0     133    1374 
   Size:        133    1241
 
SexAI A'CCwGG_T

Cuts at:      0     938    1374 
   Size:        938     436
 
SfoI GGC'GCC

Cuts at:      0    1279    1374 
   Size:       1279      95
 
SmlI C'TyrA_G

Cuts at:      0     243     295    1374 
   Size:        243      52    1079

  Fragments arranged by size: 

                1079     243      52
 
StuI AGG'CCT

Cuts at:      0     619     859    1374 
   Size:        619     240     515

  Fragments arranged by size: 

                 619     515     240
 
StyI C'CwwG_G

Cuts at:      0     264     425     472    1374 
   Size:        264     161      47     902

  Fragments arranged by size: 

                 902     264     161      47
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     406    1065    1374 
   Size:        406     659     309

  Fragments arranged by size: 

                 659     406     309
 
TatI w'GTAC_w

Cuts at:      0     121    1374 
   Size:        121    1253
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      65      97    1374 
   Size:         65      32    1277

  Fragments arranged by size: 

                1277      65      32
 

 Enzymes that do cut:

     AarI     AcuI    AflII   AflIII     AgeI     AleI     AlfI    AlwNI
     ApoI     AvaI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI     BlpI Bme1580I     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BstAPI   BstEII
    BstXI   Bsu36I     BtsI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I     KasI     MfeI     MmeI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NheI     NspI     PciI    PflMI     PfoI
    PpuMI    PvuII     SacI    SanDI     SapI    SexAI     SfoI     SmlI
     StuI     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI     AhdI     AloI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI
    BfrBI     BglI    BglII    BmgBI     BmrI    BsaAI    BsaBI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI   BssHII    BssSI    BstBI
    BstYI  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MluI     MscI     NcoI
     NdeI     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI     SbfI     ScaI     SfcI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com