Restriction map for pF1KE9527
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BcnI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsoBI       
BspDI       BspEI       BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstBI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoRI       EcoRV       EcoT22I     FauI        FseI        
FspI        HaeII       HapII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        KspI        MamI        MluI        
MroI        MspI        NaeI        NarI        NciI        
NdeI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        Tth111I     
Van91I      XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI          241  GTCTAC               GT^[AC][GT]AC
AccII          15  CGCG                 CG^CG
AlwNI         773  CAGCCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
BanI           70  GGCACC               G^G[CT][GA]CC
BanII           6  GAGCCC               G[GA]GC[CT]^C
BbsI          226  GTCTTC               GAAGAC(2/6)
BciVI        1114  GTATCC               GTATCC(6/5)
BclI          794  TGATCA               T^GATCA
BglI          343  GCCAATCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         226  GTCTTC               GAAGAC(2/6)
BseRI         364  CTCCTC               GAGGAG(10/8)
BsgI          511  GTGCAG               GTGCAG(16/14)
BsmI          800  GAATGC               GAATGC(1/-1)
Bsp1407I      974  TGTACA               T^GTACA
BspMI         499  ACCTGC               ACCTGC(4/8)
BsrDI         567  CATTGC               GCAATG(2/0)
BsrGI         974  TGTACA               T^GTACA
BstAPI        562  GCAAACATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        612  GGTCACC              G^GT[ACGT]ACC
BstPI         612  GGTCACC              G^GT[ACGT]ACC
BstUI          15  CGCG                 CG^CG
BstXI         138  CCACGTCACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI          91  AGATCC               [GA]^GATC[CT]
Cac8I          45  GCTAGC               GC[ACGT]^[ACGT]GC
CfoI           14  GCGC                 GCG^C
DrdI         1018  GACCCCTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       612  GGTCACC              G^GT[ACGT]ACC
FbaI          794  TGATCA               T^GATCA
HhaI           14  GCGC                 GCG^C
HinP1I         14  GCGC                 G^CGC
HinfI         603  GATTC                G^A[ACGT]TC
MaeII         140  ACGT                 A^CGT
MflI           91  AGATCC               [GA]^GATC[CT]
MspA1I       1008  CAGCTG               C[AC]G^C[GT]G
MvnI           15  CGCG                 CG^CG
NheI           45  GCTAGC               G^CTAGC
PshAI         219  GACCACTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI           63  CTGCAG               CTGCA^G
PvuII        1008  CAGCTG               CAG^CTG
SfcI           63  CTGCAG               C^T[GA][CT]AG
SfiI          342  GGCCAATCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI         974  TGTACA               T^GTACA
TaqI         1016  TCGA                 T^CGA
TfiI          603  GATTC                G^A[AT]TC
TthHB8I      1016  TCGA                 T^CGA
XcmI          344  CCAATCTGGCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          91  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AflIII        473  ACATGT               A^C[GA][CT]GT
              713  ACATGT              
AlwI           92  GATCC                GGATC(4/5)
             1056  GGATC               
BfaI           46  CTAG                 C^TAG
              905  CTAG                
BmyI            6  GAGCCC               G[AGT]GC[ACT]^C
              544  GGGCAC              
BpmI          549  CTCCAG               CTGGAG(16/14)
             1137  CTCCAG              
BsmAI         710  GAGAC                GTCTC(1/5)
              868  GTCTC               
Bsp1286I        6  GAGCCC               G[AGT]GC[ACT]^C
              544  GGGCAC              
BspHI         494  TCATGA               T^CATGA
              678  TCATGA              
BspLU11I      473  ACATGT               A^CATGT
              713  ACATGT              
DsaI          539  CCATGG               C^C[GA][CT]GG
              818  CCATGG              
HphI          614  TCACC                GGTGA(8/7)
             1121  TCACC               
MaeI           46  CTAG                 C^TAG
              905  CTAG                
MfeI          236  CAATTG               C^AATTG
              248  CAATTG              
MunI          236  CAATTG               C^AATTG
              248  CAATTG              
NcoI          539  CCATGG               C^CATGG
              818  CCATGG              
NspI          473  ACATGT               [GA]CATG^[CT]
              713  ACATGT              
RcaI          494  TCATGA               T^CATGA
              678  TCATGA              
Tsp45I        142  GTCAC                ^GT[GC]AC
              613  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   47 enzymes) #
#########################################

AciI        AfaI        AluI        BalI        BbvI        
BsaJI       BsiYI       BslI        BsrI        BssKI       
BstF5I      BstNI       Cfr13I      DdeI        DpnII       
EaeI        EarI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      ItaI        Ksp632I     MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MseI        MslI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        Tru9I       TseI        
Tsp509I     TspRI       
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