(Circular) (Six-base) MAPSORT of: pF1KB5995.seq Check: 140 from: 1 to: 4844 pF1KB5995 4844 bp With 182 enzymes: SgfI * October 7, 2009 14:19 .. AccI GT'mk_AC Cuts at: 251 1647 1882 251 Size: 1396 235 3213 Fragments arranged by size: 3213 1396 235 Acc65I G'GTAC_C Cuts at: 1860 1860 Size: 4844 AclI AA'CG_TT Cuts at: 4684 4684 Size: 4844 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 212 430 580 2669 3101 3848 4116 212 Size: 218 150 2089 432 747 268 940 Fragments arranged by size: 2089 940 747 432 268 218 150 AflIII A'CryG_T Cuts at: 587 2196 3300 4277 587 Size: 1609 1104 977 1154 Fragments arranged by size: 1609 1154 1104 977 AgeI A'CCGG_T Cuts at: 3232 3232 Size: 4844 AhdI GACnn_n'nnGTC Cuts at: 464 464 Size: 4844 AlwNI CAG_nnn'CTG Cuts at: 895 907 1522 2149 3716 895 Size: 12 615 627 1567 2023 Fragments arranged by size: 2023 1567 627 615 12 ApaLI G'TGCA_C Cuts at: 3614 3614 Size: 4844 ApoI r'AATT_y Cuts at: 349 674 1848 4197 4453 349 Size: 325 1174 2349 256 740 Fragments arranged by size: 2349 1174 740 325 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4844 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4844 AvaI C'yCGr_G Cuts at: 1061 1240 1328 1358 1448 1681 1864 1061 Size: 179 88 30 90 233 183 4041 Fragments arranged by size: 4041 233 183 179 90 88 30 BamHI G'GATC_C Cuts at: 654 754 1033 1323 1597 1869 654 Size: 100 279 290 274 272 3629 Fragments arranged by size: 3629 290 279 274 272 100 BanI G'GyrC_C Cuts at: 427 1860 2274 2522 2557 427 Size: 1433 414 248 35 2714 Fragments arranged by size: 2714 1433 414 248 35 BanII G_rGCy'C Cuts at: 1247 1858 2888 1247 Size: 611 1030 3203 Fragments arranged by size: 3203 1030 611 BbeI G_GCGC'C Cuts at: 2278 2526 2278 Size: 248 4596 BbvCI CC'TCA_GC Cuts at: 722 722 Size: 4844 BciVI GTATCCnnnnn_n' Cuts at: 1497 1804 2742 3503 4844 1497 Size: 307 938 761 1341 1497 Fragments arranged by size: 1497 1341 938 761 307 BclI T'GATC_A Cuts at: 2364 2364 Size: 4844 BfrBI ATG'CAT Cuts at: 819 819 Size: 4844 BglI GCCn_nnn'nGGC Cuts at: 796 1369 1667 796 Size: 573 298 3973 Fragments arranged by size: 3973 573 298 BglII A'GATC_T Cuts at: 2359 4396 2359 Size: 2037 2807 BlpI GC'TnA_GC Cuts at: 1956 1956 Size: 4844 Bme1580I G_kGCm'C Cuts at: 432 2469 2562 3618 432 Size: 2037 93 1056 1658 Fragments arranged by size: 2037 1658 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 795 1510 1556 1606 2056 2137 2472 4616 795 Size: 715 46 50 450 81 335 2144 1023 Fragments arranged by size: 2144 1023 715 450 335 81 50 46 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3211 3211 Size: 4844 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 950 1142 1850 950 Size: 192 708 3944 Fragments arranged by size: 3944 708 192 Bpu10I CC'TnA_GC Cuts at: 722 750 921 947 1778 722 Size: 28 171 26 831 3788 Fragments arranged by size: 3788 831 171 28 26 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2020 2899 3391 3689 2020 Size: 879 492 298 3175 Fragments arranged by size: 3175 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 122 451 4548 122 Size: 329 4097 418 Fragments arranged by size: 4097 418 329 BsaAI yAC'GTr Cuts at: 2197 2197 Size: 4844 BsaBI GATnn'nnATC Cuts at: 6 2358 4395 6 Size: 2352 2037 455 Fragments arranged by size: 2352 2037 455 BsaHI Gr'CG_yC Cuts at: 2275 2523 3225 4721 2275 Size: 248 702 1496 2398 Fragments arranged by size: 2398 1496 702 248 BsaWI w'CCGG_w Cuts at: 657 2033 2554 3232 3506 3653 4064 657 Size: 1376 521 678 274 147 411 1437 Fragments arranged by size: 1437 1376 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1823 1853 1823 Size: 30 4814 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 827 947 827 Size: 120 4724 BseYI C'CCAG_C Cuts at: 132 739 2269 3027 3604 4100 132 Size: 607 1530 758 577 496 876 Fragments arranged by size: 1530 876 758 607 577 496 BsiEI CG_ry'CG Cuts at: 104 2386 2432 3640 104 Size: 2282 46 1208 1308 Fragments arranged by size: 2282 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 366 1836 1858 2636 2826 3618 366 Size: 1470 22 778 190 792 1592 Fragments arranged by size: 1592 1470 792 778 190 22 BsmI GAATG_Cn' Cuts at: 821 944 962 1556 1637 821 Size: 123 18 594 81 4028 Fragments arranged by size: 4028 594 123 81 18 Bsp1286I G_dGCh'C Cuts at: 366 432 1247 1836 1858 2469 2562 2636 2826 Size: 66 815 589 22 611 93 74 190 Cuts at: 2826 2888 3618 366 Size: 62 730 1592 Fragments arranged by size: 1592 815 730 611 589 190 93 74 66 62 22 BspEI T'CCGG_A Cuts at: 657 4064 657 Size: 3407 1437 BspHI T'CATG_A Cuts at: 1710 4020 1710 Size: 2310 2534 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1725 1894 2108 2410 2791 1725 Size: 169 214 302 381 3778 Fragments arranged by size: 3778 381 302 214 169 BsrBI CCG'CTC Cuts at: 50 3136 3190 4674 4839 50 Size: 3086 54 1484 165 55 Fragments arranged by size: 3086 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 1415 1445 1475 2756 1415 Size: 30 30 1281 3503 Fragments arranged by size: 3503 1281 30 30 BsrFI r'CCGG_y Cuts at: 2842 3023 3232 2842 Size: 181 209 4454 Fragments arranged by size: 4454 209 181 BssSI C'ACGA_G Cuts at: 1343 1433 3115 3473 1343 Size: 90 1682 358 2714 Fragments arranged by size: 2714 1682 358 90 BstAPI GCAn_nnn'nTGC Cuts at: 322 2221 322 Size: 1899 2945 BstBI TT'CG_AA Cuts at: 3205 3205 Size: 4844 BstXI CCAn_nnnn'nTGG Cuts at: 739 1186 1500 739 Size: 447 314 4083 Fragments arranged by size: 4083 447 314 BstYI r'GATC_y Cuts at: 1 654 754 1033 1323 1597 1869 2336 2359 Size: 653 100 279 290 274 272 467 23 Cuts at: 2359 2694 2940 3952 4038 4050 4396 1 Size: 335 246 1012 86 12 346 449 Fragments arranged by size: 1012 653 467 449 346 335 290 279 274 272 246 100 86 23 12 BstZ17I GTA'TAC Cuts at: 252 1648 252 Size: 1396 3448 Bsu36I CC'TnA_GG Cuts at: 470 785 794 470 Size: 315 9 4520 Fragments arranged by size: 4520 315 9 BtgI C'CryG_G Cuts at: 107 732 980 1093 1364 107 Size: 625 248 113 271 3587 Fragments arranged by size: 3587 625 271 248 113 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 667 1438 1468 2794 2975 3138 667 Size: 771 30 1326 181 163 2373 Fragments arranged by size: 2373 1326 771 181 163 30 BtsI GCAGTG_nn' Cuts at: 2087 2087 Size: 4844 DraI TTT'AAA Cuts at: 1805 1843 1805 Size: 38 4806 DraIII CAC_nnn'GTG Cuts at: 322 322 Size: 4844 DrdI GACnn_nn'nnGTC Cuts at: 2550 3408 2550 Size: 858 3986 EaeI y'GGCC_r Cuts at: 1188 2429 2603 2994 3021 4366 1188 Size: 1241 174 391 27 1345 1666 Fragments arranged by size: 1666 1345 1241 391 174 27 EagI C'GGCC_G Cuts at: 2429 2429 Size: 4844 EarI CTCTTCn'nnn_ Cuts at: 188 398 406 2867 3077 188 Size: 210 8 2461 210 1955 Fragments arranged by size: 2461 1955 210 210 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 3052 3362 3508 4654 3052 Size: 310 146 1146 3242 Fragments arranged by size: 3242 1146 310 146 EcoICRI GAG'CTC Cuts at: 1856 1856 Size: 4844 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 212 430 580 950 1142 1850 2669 3101 3848 Size: 218 150 370 192 708 819 432 747 Cuts at: 3848 4116 212 Size: 268 940 Fragments arranged by size: 940 819 747 708 432 370 268 218 192 150 EcoNI CCTnn'n_nnAGG Cuts at: 749 932 749 Size: 183 4661 EcoO109I rG'GnC_Cy Cuts at: 789 904 1273 1534 1558 1608 1983 789 Size: 115 369 261 24 50 375 3650 Fragments arranged by size: 3650 375 369 261 115 50 24 EcoRI G'AATT_C Cuts at: 349 1848 349 Size: 1499 3345 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 228 2863 228 Size: 2635 2209 FspI TGC'GCA Cuts at: 2625 2625 Size: 4844 HaeII r_GCGC'y Cuts at: 2278 2526 3548 4527 2278 Size: 248 1022 979 2595 Fragments arranged by size: 2595 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 446 450 478 482 1416 1448 2686 2716 2718 Size: 4 28 4 934 32 1238 30 2 Cuts at: 2718 2748 446 Size: 30 2542 Fragments arranged by size: 2542 1238 934 32 30 30 28 4 4 2 HincII GTy'rAC Cuts at: 1883 1883 Size: 4844 HindIII A'AGCT_T Cuts at: 848 848 Size: 4844 KasI G'GCGC_C Cuts at: 2274 2522 2274 Size: 248 4596 KpnI G_GTAC'C Cuts at: 1864 1864 Size: 4844 MluI A'CGCG_T Cuts at: 4277 4277 Size: 4844 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 204 933 3515 3699 204 Size: 729 2582 184 1349 Fragments arranged by size: 2582 1349 729 184 MscI TGG'CCA Cuts at: 1190 2605 1190 Size: 1415 3429 MslI CAynn'nnrTG Cuts at: 824 2833 2960 824 Size: 2009 127 2708 Fragments arranged by size: 2708 2009 127 MspA1I CmG'CkG Cuts at: 136 892 1029 1955 2269 2629 3642 3887 136 Size: 756 137 926 314 360 1013 245 1093 Fragments arranged by size: 1093 1013 926 756 360 314 245 137 NaeI GCC'GGC Cuts at: 3025 3025 Size: 4844 NarI GG'CG_CC Cuts at: 2275 2523 2275 Size: 248 4596 NcoI C'CATG_G Cuts at: 107 732 980 1364 107 Size: 625 248 384 3587 Fragments arranged by size: 3587 625 384 248 NgoMIV G'CCGG_C Cuts at: 3023 3023 Size: 4844 NsiI A_TGCA'T Cuts at: 821 821 Size: 4844 NspI r_CATG'y Cuts at: 591 823 1897 2397 3304 591 Size: 232 1074 500 907 2131 Fragments arranged by size: 2131 1074 907 500 232 PasI CC'CwG_GG Cuts at: 1593 1593 Size: 4844 PciI A'CATG_T Cuts at: 587 3300 587 Size: 2713 2131 PflMI CCAn_nnn'nTGG Cuts at: 733 807 967 1522 733 Size: 74 160 555 4055 Fragments arranged by size: 4055 555 160 74 PfoI T'CCnGG_A Cuts at: 927 927 Size: 4844 PmeI GTTT'AAAC Cuts at: 1843 1843 Size: 4844 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3983 4015 3983 Size: 32 4812 PpuMI rG'GwC_Cy Cuts at: 904 1273 1534 1558 1608 904 Size: 369 261 24 50 4140 Fragments arranged by size: 4140 369 261 50 24 PshAI GACnn'nnGTC Cuts at: 975 975 Size: 4844 PspXI vC'TCGA_Gb Cuts at: 1061 1061 Size: 4844 PstI C_TGCA'G Cuts at: 1755 1891 2576 1755 Size: 136 685 4023 Fragments arranged by size: 4023 685 136 PvuI CG_AT'CG Cuts at: 104 104 Size: 4844 PvuII CAG'CTG Cuts at: 892 2269 2629 892 Size: 1377 360 3107 Fragments arranged by size: 3107 1377 360 RsrII CG'GwC_CG Cuts at: 3039 3039 Size: 4844 SacI G_AGCT'C Cuts at: 1858 1858 Size: 4844 SalI G'TCGA_C Cuts at: 1881 1881 Size: 4844 SanDI GG'GwC_CC Cuts at: 1558 1558 Size: 4844 SapI GCTCTTCn'nnn_ Cuts at: 188 398 406 2867 3077 188 Size: 210 8 2461 210 1955 Fragments arranged by size: 2461 1955 210 210 8 SbfI CC_TGCA'GG Cuts at: 1891 1891 Size: 4844 ScaI AGT'ACT Cuts at: 4361 4361 Size: 4844 SfcI C'TryA_G Cuts at: 33 1751 1887 2572 3565 3756 33 Size: 1718 136 685 993 191 1121 Fragments arranged by size: 1718 1121 993 685 191 136 SfiI GGCCn_nnn'nGGCC Cuts at: 796 796 Size: 4844 SfoI GGC'GCC Cuts at: 2276 2524 2276 Size: 248 4596 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4844 SmaI CCC'GGG Cuts at: 1683 1866 1683 Size: 183 4661 SmlI C'TyrA_G Cuts at: 1061 1999 2914 3406 3668 1061 Size: 938 915 492 262 2237 Fragments arranged by size: 2237 938 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4844 SphI G_CATG'C Cuts at: 1897 2397 1897 Size: 500 4344 StyI C'CwwG_G Cuts at: 107 417 732 980 1301 1364 1978 2332 107 Size: 310 315 248 321 63 614 354 2619 Fragments arranged by size: 2619 614 354 321 315 310 248 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 799 1185 2427 2560 3019 3228 799 Size: 386 1242 133 459 209 2415 Fragments arranged by size: 2415 1242 459 386 209 133 TatI w'GTAC_w Cuts at: 4359 4359 Size: 4844 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 746 778 4361 4393 746 Size: 32 3583 32 1197 Fragments arranged by size: 3583 1197 32 32 Tth111I GACn'n_nGTC Cuts at: 2641 2641 Size: 4844 XbaI T'CTAG_A Cuts at: 1875 1875 Size: 4844 XcmI CCAnnnn_n'nnnnTGG Cuts at: 808 1254 2339 808 Size: 446 1085 3313 Fragments arranged by size: 3313 1085 446 XhoI C'TCGA_G Cuts at: 1061 1061 Size: 4844 XmaI C'CCGG_G Cuts at: 1681 1864 1681 Size: 183 4661 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AleI AlfI AloI ApaI AscI AvrII BaeI BbsI BcgI BmgBI BmtI BsgI BsiWI BsmBI BsrGI BssHII BstEII ClaI CspCI EcoRV FseI FspAI HpaI MfeI NdeI NheI NotI NruI PacI PmlI PsiI PspOMI PsrI SacII SexAI SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI