Restriction map for
pF1KB5995
(Linear) (Six-base) MAPSORT of: KIBB5995.seq Check: 4435 from: 1 to: 1731
KIBB5995.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 0 143 1539 1731
Size: 143 1396 192
Fragments arranged by size:
1396 192 143
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 322 472 1731
Size: 104 218 150 1259
Fragments arranged by size:
1259 218 150 104
AflIII A'CryG_T
Cuts at: 0 479 1731
Size: 479 1252
AhdI GACnn_n'nnGTC
Cuts at: 0 356 1731
Size: 356 1375
AlwNI CAG_nnn'CTG
Cuts at: 0 787 799 1414 1731
Size: 787 12 615 317
Fragments arranged by size:
787 615 317 12
ApoI r'AATT_y
Cuts at: 0 241 566 1731
Size: 241 325 1165
Fragments arranged by size:
1165 325 241
AvaI C'yCGr_G
Cuts at: 0 953 1132 1220 1250 1340 1573 1731
Size: 953 179 88 30 90 233 158
Fragments arranged by size:
953 233 179 158 90 88 30
BamHI G'GATC_C
Cuts at: 0 546 646 925 1215 1489 1731
Size: 546 100 279 290 274 242
Fragments arranged by size:
546 290 279 274 242 100
BanI G'GyrC_C
Cuts at: 0 319 1731
Size: 319 1412
BanII G_rGCy'C
Cuts at: 0 1139 1731
Size: 1139 592
BbvCI CC'TCA_GC
Cuts at: 0 614 1731
Size: 614 1117
BciVI GTATCCnnnnn_n'
Cuts at: 0 1389 1696 1731
Size: 1389 307 35
Fragments arranged by size:
1389 307 35
BfrBI ATG'CAT
Cuts at: 0 711 1731
Size: 711 1020
BglI GCCn_nnn'nGGC
Cuts at: 0 688 1261 1559 1731
Size: 688 573 298 172
Fragments arranged by size:
688 573 298 172
Bme1580I G_kGCm'C
Cuts at: 0 324 1731
Size: 324 1407
BmrI ACTGGGnnnn_n'
Cuts at: 0 687 1402 1448 1498 1731
Size: 687 715 46 50 233
Fragments arranged by size:
715 687 233 50 46
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 842 1034 1731
Size: 842 192 697
Fragments arranged by size:
842 697 192
Bpu10I CC'TnA_GC
Cuts at: 0 614 642 813 839 1670 1731
Size: 614 28 171 26 831 61
Fragments arranged by size:
831 614 171 61 28 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 14 343 1731
Size: 14 329 1388
Fragments arranged by size:
1388 329 14
BsaWI w'CCGG_w
Cuts at: 0 549 1731
Size: 549 1182
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 719 839 1731
Size: 719 120 892
Fragments arranged by size:
892 719 120
BseYI C'CCAG_C
Cuts at: 0 24 631 1731
Size: 24 607 1100
Fragments arranged by size:
1100 607 24
BsiHKAI G_wGCw'C
Cuts at: 0 258 1728 1731
Size: 258 1470 3
Fragments arranged by size:
1470 258 3
BsmI GAATG_Cn'
Cuts at: 0 713 836 854 1448 1529 1731
Size: 713 123 18 594 81 202
Fragments arranged by size:
713 594 202 123 81 18
Bsp1286I G_dGCh'C
Cuts at: 0 258 324 1139 1728 1731
Size: 258 66 815 589 3
Fragments arranged by size:
815 589 258 66 3
BspEI T'CCGG_A
Cuts at: 0 549 1731
Size: 549 1182
BspHI T'CATG_A
Cuts at: 0 1602 1731
Size: 1602 129
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1617 1731
Size: 1617 114
BsrDI GCAATG_nn'
Cuts at: 0 1307 1337 1367 1731
Size: 1307 30 30 364
Fragments arranged by size:
1307 364 30 30
BssSI C'ACGA_G
Cuts at: 0 1235 1325 1731
Size: 1235 90 406
Fragments arranged by size:
1235 406 90
BstAPI GCAn_nnn'nTGC
Cuts at: 0 214 1731
Size: 214 1517
BstXI CCAn_nnnn'nTGG
Cuts at: 0 631 1078 1392 1731
Size: 631 447 314 339
Fragments arranged by size:
631 447 339 314
BstYI r'GATC_y
Cuts at: 0 546 646 925 1215 1489 1731
Size: 546 100 279 290 274 242
Fragments arranged by size:
546 290 279 274 242 100
BstZ17I GTA'TAC
Cuts at: 0 144 1540 1731
Size: 144 1396 191
Fragments arranged by size:
1396 191 144
Bsu36I CC'TnA_GG
Cuts at: 0 362 677 686 1731
Size: 362 315 9 1045
Fragments arranged by size:
1045 362 315 9
BtgI C'CryG_G
Cuts at: 0 624 872 985 1256 1731
Size: 624 248 113 271 475
Fragments arranged by size:
624 475 271 248 113
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 559 1330 1360 1731
Size: 559 771 30 371
Fragments arranged by size:
771 559 371 30
DraI TTT'AAA
Cuts at: 0 1697 1731
Size: 1697 34
DraIII CAC_nnn'GTG
Cuts at: 0 214 1731
Size: 214 1517
EaeI y'GGCC_r
Cuts at: 0 1080 1731
Size: 1080 651
EarI CTCTTCn'nnn_
Cuts at: 0 80 290 298 1731
Size: 80 210 8 1433
Fragments arranged by size:
1433 210 80 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 322 472 842 1034 1731
Size: 104 218 150 370 192 697
Fragments arranged by size:
697 370 218 192 150 104
EcoNI CCTnn'n_nnAGG
Cuts at: 0 641 824 1731
Size: 641 183 907
Fragments arranged by size:
907 641 183
EcoO109I rG'GnC_Cy
Cuts at: 0 681 796 1165 1426 1450 1500 1731
Size: 681 115 369 261 24 50 231
Fragments arranged by size:
681 369 261 231 115 50 24
EcoRI G'AATT_C
Cuts at: 0 241 1731
Size: 241 1490
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 120 1731
Size: 120 1611
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 338 342 370 374 1308 1340 1731
Size: 338 4 28 4 934 32 391
Fragments arranged by size:
934 391 338 32 28 4 4
HindIII A'AGCT_T
Cuts at: 0 740 1731
Size: 740 991
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 825 1731
Size: 96 729 906
Fragments arranged by size:
906 729 96
MscI TGG'CCA
Cuts at: 0 1082 1731
Size: 1082 649
MslI CAynn'nnrTG
Cuts at: 0 716 1731
Size: 716 1015
MspA1I CmG'CkG
Cuts at: 0 28 784 921 1731
Size: 28 756 137 810
Fragments arranged by size:
810 756 137 28
NcoI C'CATG_G
Cuts at: 0 624 872 1256 1731
Size: 624 248 384 475
Fragments arranged by size:
624 475 384 248
NsiI A_TGCA'T
Cuts at: 0 713 1731
Size: 713 1018
NspI r_CATG'y
Cuts at: 0 483 715 1731
Size: 483 232 1016
Fragments arranged by size:
1016 483 232
PasI CC'CwG_GG
Cuts at: 0 1485 1731
Size: 1485 246
PciI A'CATG_T
Cuts at: 0 479 1731
Size: 479 1252
PflMI CCAn_nnn'nTGG
Cuts at: 0 625 699 859 1414 1731
Size: 625 74 160 555 317
Fragments arranged by size:
625 555 317 160 74
PfoI T'CCnGG_A
Cuts at: 0 819 1731
Size: 819 912
PpuMI rG'GwC_Cy
Cuts at: 0 796 1165 1426 1450 1500 1731
Size: 796 369 261 24 50 231
Fragments arranged by size:
796 369 261 231 50 24
PshAI GACnn'nnGTC
Cuts at: 0 867 1731
Size: 867 864
PspXI vC'TCGA_Gb
Cuts at: 0 953 1731
Size: 953 778
PstI C_TGCA'G
Cuts at: 0 1647 1731
Size: 1647 84
PvuII CAG'CTG
Cuts at: 0 784 1731
Size: 784 947
SanDI GG'GwC_CC
Cuts at: 0 1450 1731
Size: 1450 281
SapI GCTCTTCn'nnn_
Cuts at: 0 80 290 298 1731
Size: 80 210 8 1433
Fragments arranged by size:
1433 210 80 8
SfcI C'TryA_G
Cuts at: 0 1643 1731
Size: 1643 88
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 688 1731
Size: 688 1043
SmaI CCC'GGG
Cuts at: 0 1575 1731
Size: 1575 156
SmlI C'TyrA_G
Cuts at: 0 953 1731
Size: 953 778
StyI C'CwwG_G
Cuts at: 0 309 624 872 1193 1256 1731
Size: 309 315 248 321 63 475
Fragments arranged by size:
475 321 315 309 248 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 691 1077 1731
Size: 691 386 654
Fragments arranged by size:
691 654 386
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 638 670 1731
Size: 638 32 1061
Fragments arranged by size:
1061 638 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 700 1146 1731
Size: 700 446 585
Fragments arranged by size:
700 585 446
XhoI C'TCGA_G
Cuts at: 0 953 1731
Size: 953 778
XmaI C'CCGG_G
Cuts at: 0 1573 1731
Size: 1573 158
Enzymes that do cut:
AccI AcuI AflIII AhdI AlwNI ApoI AvaI BamHI
BanI BanII BbvCI BciVI BfrBI BglI Bme1580I BmrI
BpmI Bpu10I BsaI BsaWI BseRI BseYI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrDI BssSI BstAPI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI DraI DraIII EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HindIII
MmeI MscI MslI MspA1I NcoI NsiI NspI PasI
PciI PflMI PfoI PpuMI PshAI PspXI PstI PvuII
SanDI SapI SfcI SfiI SmaI SmlI StyI TaqII
TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BcgI BclI BglII BlpI BmgBI
BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaXI BsgI
BsiEI BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BstBI
BstEII BtsI ClaI CspCI DrdI EagI EciI EcoICRI
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PmeI PmlI PpiI PsiI PspOMI
PsrI PvuI RsrII SacI SacII SalI SbfI ScaI
SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XmnI ZraI