Restriction map for pF1KB6521
 (Circular) (Six-base) MAPSORT of: pF1KB6521.seq  Check: 29  from: 1  to: 4202



pF1KB6521  4202 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AccI GT'mk_AC

Cuts at:   1240    1240 
   Size:       4202
 
Acc65I G'GTAC_C

Cuts at:   1218    1218 
   Size:       4202
 
AclI AA'CG_TT

Cuts at:   4042    4042 
   Size:       4202
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   2027    2459    3206    3474    2027 
   Size:        432     747     268    2755

  Fragments arranged by size: 

                2755     747     432     268
 
AfeI AGC'GCT

Cuts at:    849     849 
   Size:       4202
 
AflII C'TTAA_G

Cuts at:    819     819 
   Size:       4202
 
AflIII A'CryG_T

Cuts at:   1554    2658    3635    1554 
   Size:       1104     977    2121

  Fragments arranged by size: 

                2121    1104     977
 
AgeI A'CCGG_T

Cuts at:   2590    2590 
   Size:       4202
 
AlwNI CAG_nnn'CTG

Cuts at:   1507    3074    1507 
   Size:       1567    2635
 
ApaLI G'TGCA_C

Cuts at:   2972    2972 
   Size:       4202
 
ApoI r'AATT_y

Cuts at:    480     694     722    1206    3555    3811     480 
   Size:        214      28     484    2349     256     871

  Fragments arranged by size: 

                2349     871     484     256     214      28
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4202
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4202
 
AvaI C'yCGr_G

Cuts at:   1222    1222 
   Size:       4202
 
BamHI G'GATC_C

Cuts at:   1227    1227 
   Size:       4202
 
BanI G'GyrC_C

Cuts at:   1218    1632    1880    1915    1218 
   Size:        414     248      35    3505

  Fragments arranged by size: 

                3505     414     248      35
 
BanII G_rGCy'C

Cuts at:   1216    2246    1216 
   Size:       1030    3172
 
BbeI G_GCGC'C

Cuts at:   1636    1884    1636 
   Size:        248    3954
 
BbvCI CC'TCA_GC

Cuts at:    576     576 
   Size:       4202
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124      90 
   Size:         34    4168
 
BciVI GTATCCnnnnn_n'

Cuts at:   2100    2861    4202    2100 
   Size:        761    1341    2100

  Fragments arranged by size: 

                2100    1341     761
 
BclI T'GATC_A

Cuts at:   1722    1722 
   Size:       4202
 
BfrBI ATG'CAT

Cuts at:    436     436 
   Size:       4202
 
BglII A'GATC_T

Cuts at:    315    1717    3754     315 
   Size:       1402    2037     763

  Fragments arranged by size: 

                2037    1402     763
 
BlpI GC'TnA_GC

Cuts at:   1314    1314 
   Size:       4202
 
Bme1580I G_kGCm'C

Cuts at:   1827    1920    2976    1827 
   Size:         93    1056    3053

  Fragments arranged by size: 

                3053    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1414    1495    1830    3974    1414 
   Size:         81     335    2144    1642

  Fragments arranged by size: 

                2144    1642     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2569    2569 
   Size:       4202
 
Bpu10I CC'TnA_GC

Cuts at:    576     584     576 
   Size:          8    4194
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    445    1378    2257    2749    3047     445 
   Size:        933     879     492     298    1600

  Fragments arranged by size: 

                1600     933     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3906    3906 
   Size:       4202
 
BsaAI yAC'GTr

Cuts at:   1555    1555 
   Size:       4202
 
BsaBI GATnn'nnATC

Cuts at:      6    1716    3753       6 
   Size:       1710    2037     455

  Fragments arranged by size: 

                2037    1710     455
 
BsaHI Gr'CG_yC

Cuts at:   1633    1881    2583    4079    1633 
   Size:        248     702    1496    1756

  Fragments arranged by size: 

                1756    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1391    1912    2590    2864    3011    3422    1391 
   Size:        521     678     274     147     411    2171

  Fragments arranged by size: 

                2171     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    601     631     601 
   Size:         30    4172
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    182     182 
   Size:       4202
 
BseYI C'CCAG_C

Cuts at:    904    1627    2385    2962    3458     904 
   Size:        723     758     577     496    1648

  Fragments arranged by size: 

                1648     758     723     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1744    1790    2998     104 
   Size:       1640      46    1208    1308

  Fragments arranged by size: 

                1640    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1216    1994    2184    2976    1216 
   Size:        778     190     792    2442

  Fragments arranged by size: 

                2442     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:   1216    1827    1920    1994    2184    2246    2976    1216 
   Size:        611      93      74     190      62     730    2442

  Fragments arranged by size: 

                2442     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3422    3422 
   Size:       4202
 
BspHI T'CATG_A

Cuts at:   3378    3378 
   Size:       4202
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1252    1466    1768    2149    1252 
   Size:        214     302     381    3305

  Fragments arranged by size: 

                3305     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2494    2548    4032    4197      50 
   Size:       2444      54    1484     165      55

  Fragments arranged by size: 

                2444    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    746     899    2114     746 
   Size:        153    1215    2834

  Fragments arranged by size: 

                2834    1215     153
 
BsrFI r'CCGG_y

Cuts at:   2200    2381    2590    2200 
   Size:        181     209    3812

  Fragments arranged by size: 

                3812     209     181
 
BssSI C'ACGA_G

Cuts at:   2473    2831    2473 
   Size:        358    3844
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1129    1579    1129 
   Size:        450    3752
 
BstBI TT'CG_AA

Cuts at:   2563    2563 
   Size:       4202
 
BstXI CCAn_nnnn'nTGG

Cuts at:    372     372 
   Size:       4202
 
BstYI r'GATC_y

Cuts at:      1     315    1227    1694    1717    2052    2298    3310    3396 
   Size:        314     912     467      23     335     246    1012      86

Cuts at:   3396    3408    3754       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     912     467     449     346     335     314     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    605     605 
   Size:       4202
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2152    2333    2496    2152 
   Size:        181     163    3858

  Fragments arranged by size: 

                3858     181     163
 
BtsI GCAGTG_nn'

Cuts at:    293    1445     293 
   Size:       1152    3050
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    233     268     233 
   Size:         35    4167
 
DraI TTT'AAA

Cuts at:   1201    1201 
   Size:       4202
 
DrdI GACnn_nn'nnGTC

Cuts at:   1908    2766    1908 
   Size:        858    3344
 
EaeI y'GGCC_r

Cuts at:   1787    1961    2352    2379    3724    1787 
   Size:        174     391      27    1345    2265

  Fragments arranged by size: 

                2265    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1787    1787 
   Size:       4202
 
EarI CTCTTCn'nnn_

Cuts at:   2225    2435    2225 
   Size:        210    3992
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    521    2410    2720    2866    4012     521 
   Size:       1889     310     146    1146     711

  Fragments arranged by size: 

                1889    1146     711     310     146
 
EcoICRI GAG'CTC

Cuts at:   1214    1214 
   Size:       4202
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   2027    2459    3206    3474    2027 
   Size:        432     747     268    2755

  Fragments arranged by size: 

                2755     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:   1341    1341 
   Size:       4202
 
EcoRI G'AATT_C

Cuts at:   1206    1206 
   Size:       4202
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    136    2221     136 
   Size:       2085    2117
 
FspI TGC'GCA

Cuts at:   1983    1983 
   Size:       4202
 
HaeII r_GCGC'y

Cuts at:    851    1636    1884    2906    3885     851 
   Size:        785     248    1022     979    1168

  Fragments arranged by size: 

                1168    1022     979     785     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    147     179     601     633    2044    2074    2076    2106     147 
   Size:         32     422      32    1411      30       2      30    2243

  Fragments arranged by size: 

                2243    1411     422      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:   1110    1241    1110 
   Size:        131    4071
 
KasI G'GCGC_C

Cuts at:   1632    1880    1632 
   Size:        248    3954
 
KpnI G_GTAC'C

Cuts at:   1222    1222 
   Size:       4202
 
MluI A'CGCG_T

Cuts at:   3635    3635 
   Size:       4202
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2873    3057    2873 
   Size:        184    4018
 
MscI TGG'CCA

Cuts at:   1963    1963 
   Size:       4202
 
MslI CAynn'nnrTG

Cuts at:   2191    2318    2191 
   Size:        127    4075
 
MspA1I CmG'CkG

Cuts at:    153     580     919    1313    1627    1987    3000    3245     153 
   Size:        427     339     394     314     360    1013     245    1110

  Fragments arranged by size: 

                1110    1013     427     394     360     339     314     245
            
 
NaeI GCC'GGC

Cuts at:   2383    2383 
   Size:       4202
 
NarI GG'CG_CC

Cuts at:   1633    1881    1633 
   Size:        248    3954
 
NcoI C'CATG_G

Cuts at:    605     605 
   Size:       4202
 
NgoMIV G'CCGG_C

Cuts at:   2381    2381 
   Size:       4202
 
NsiI A_TGCA'T

Cuts at:    438     438 
   Size:       4202
 
NspI r_CATG'y

Cuts at:   1255    1755    2662    1255 
   Size:        500     907    2795

  Fragments arranged by size: 

                2795     907     500
 
PasI CC'CwG_GG

Cuts at:    224     224 
   Size:       4202
 
PciI A'CATG_T

Cuts at:   2658    2658 
   Size:       4202
 
PflMI CCAn_nnn'nTGG

Cuts at:   1044    1044 
   Size:       4202
 
PfoI T'CCnGG_A

Cuts at:    269     269 
   Size:       4202
 
PmeI GTTT'AAAC

Cuts at:   1201    1201 
   Size:       4202
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3341    3373    3341 
   Size:         32    4170
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:    437     469     437 
   Size:         32    4170
 
PstI C_TGCA'G

Cuts at:   1249    1934    1249 
   Size:        685    3517
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4202
 
PvuII CAG'CTG

Cuts at:    580     919    1627    1987     580 
   Size:        339     708     360    2795

  Fragments arranged by size: 

                2795     708     360     339
 
RsrII CG'GwC_CG

Cuts at:   2397    2397 
   Size:       4202
 
SacI G_AGCT'C

Cuts at:   1216    1216 
   Size:       4202
 
SalI G'TCGA_C

Cuts at:   1239    1239 
   Size:       4202
 
SapI GCTCTTCn'nnn_

Cuts at:   2225    2435    2225 
   Size:        210    3992
 
SbfI CC_TGCA'GG

Cuts at:   1249    1249 
   Size:       4202
 
ScaI AGT'ACT

Cuts at:    141    3719     141 
   Size:       3578     624
 
SfcI C'TryA_G

Cuts at:     33    1245    1930    2923    3114      33 
   Size:       1212     685     993     191    1121

  Fragments arranged by size: 

                1212    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:   1634    1882    1634 
   Size:        248    3954
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4202
 
SmaI CCC'GGG

Cuts at:   1224    1224 
   Size:       4202
 
SmlI C'TyrA_G

Cuts at:    424     819    1357    2272    2764    3026     424 
   Size:        395     538     915     492     262    1600

  Fragments arranged by size: 

                1600     915     538     492     395     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4202
 
SphI G_CATG'C

Cuts at:   1255    1755    1255 
   Size:        500    3702
 
StyI C'CwwG_G

Cuts at:    158     605    1336    1690     158 
   Size:        447     731     354    2670

  Fragments arranged by size: 

                2670     731     447     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    216    1785    1918    2377    2586     216 
   Size:       1569     133     459     209    1832

  Fragments arranged by size: 

                1832    1569     459     209     133
 
TatI w'GTAC_w

Cuts at:    139    3717     139 
   Size:       3578     624
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3719    3751    3719 
   Size:         32    4170
 
Tth111I GACn'n_nGTC

Cuts at:   1999    1999 
   Size:       4202
 
XbaI T'CTAG_A

Cuts at:    682    1233     682 
   Size:        551    3651
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1697    1697 
   Size:       4202
 
XmaI C'CCGG_G

Cuts at:   1222    1222 
   Size:       4202
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI    CspCI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NsiI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AhdI     AleI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI     BbsI     BglI    BmgBI     BmtI     BpmI     BsgI
    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I
     ClaI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NdeI     NheI     NotI     NruI     PacI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI
     ZraI
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