################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MfeI MflI MluI MluNI MroI MscI MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 331 CGCG CG^CG AciI 701 GCGG CCGC(-3/-1) BanII 242 GGGCTC G[GA]GC[CT]^C BbvI 199 GCAGC GCAGC(8/12) BmyI 242 GGGCTC G[AGT]GC[ACT]^C BsaBI 541 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 704 GAGACC GGTCTC(1/5) BseRI 107 CTCCTC GAGGAG(10/8) BsmFI 225 GTCCC GGGAC(10/14) Bsp1286I 242 GGGCTC G[AGT]GC[ACT]^C BstEII 337 GGTGACC G^GT[ACGT]ACC BstPI 337 GGTGACC G^GT[ACGT]ACC BstUI 331 CGCG CG^CG DpnII 541 GATC ^GATC DraII 223 AGGTCCC [GA]G^G[ACGT]CC[CT] DrdI 523 GACAGAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 223 AGGTCCC [GA]G^G[ACGT]CC[CT] EcoO65I 337 GGTGACC G^GT[ACGT]ACC Fnu4HI 199 GCAGC GC^[ACGT]GC HincII 192 GTTGAC GT[CT]^[GA]AC HindII 192 GTTGAC GT[CT]^[GA]AC ItaI 199 GCAGC GC^[ACGT]GC MamI 541 GATCGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 541 GATC ^GATC MslI 469 CACAATAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 355 CAGCTG C[AC]G^C[GT]G MvnI 331 CGCG CG^CG MwoI 169 GCTTTGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 541 GATC ^GATC PpuMI 223 AGGTCCC [GA]G^G[AT]CC[CT] PstI 197 CTGCAG CTGCA^G PvuII 355 CAGCTG CAG^CTG SapI 262 GAAGAGC GCTCTTC(1/4) Sau3AI 541 GATC ^GATC TaqI 543 TCGA T^CGA TfiI 631 GATTC G^A[AT]TC TseI 199 GCAGC G^C[AT]GC TspRI 205 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 543 TCGA T^CGA XcmI 228 CCAGGTCATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AluI 201 AGCT AG^CT 356 AGCT BsiYI 406 CCTAAAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 565 CCTGTGATTGG BslI 406 CCTAAAAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 565 CCTGTGATTGG BsmAI 614 GAGAC GTCTC(1/5) 704 GAGAC BsrDI 536 GCAATG GCAATG(2/0) 623 GCAATG BsrI 185 ACTGG ACTGG(1/-1) 755 ACTGG BssSI 618 CTCGTG CACGAG(-5/-1) 710 CTCGTG BstF5I 26 CATCC GGATG(2/0) 417 GGATG EarI 262 GAAGAG CTCTTC(1/4) 611 GAAGAG FokI 26 CATCC GGATG(9/13) 417 GGATG HaeIII 685 GGCC GG^CC 716 GGCC HphI 337 GGTGA GGTGA(8/7) 512 TCACC Ksp632I 262 GAAGAG CTCTTC(1/4) 611 GAAGAG NlaIV 224 GGTCCC GG[ACGT]^[ACGT]CC 433 GGAACC PleI 465 GACTC GAGTC(4/5) 616 GACTC SfaNI 25 GCATC GCATC(5/9) 478 GCATC SfcI 12 CTATAG C^T[GA][CT]AG 197 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AvaII BsaJI BssKI BstNI Cfr13I DdeI EcoRII HinfI MaeIII MboII MnlI MseI MvaI NlaIII Sau96I ScrFI Tru9I Tsp45I Tsp509I