Restriction map for pF1KB0922
 (Linear) (Six-base) MAPSORT of: KIBB0922.seq  Check: 9968  from: 1  to: 1035



KIBB0922.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:14  ..

Acc65I G'GTAC_C

Cuts at:      0     940    1035 
   Size:        940      95
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1011    1035 
   Size:       1011      24
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     547     970    1035 
   Size:        547     423      65

  Fragments arranged by size: 

                 547     423      65
 
AlwNI CAG_nnn'CTG

Cuts at:      0     552     808    1035 
   Size:        552     256     227

  Fragments arranged by size: 

                 552     256     227
 
ApaI G_GGCC'C

Cuts at:      0     240     254     902    1035 
   Size:        240      14     648     133

  Fragments arranged by size: 

                 648     240     133      14
 
ApaLI G'TGCA_C

Cuts at:      0     145    1035 
   Size:        145     890
 
ApoI r'AATT_y

Cuts at:      0      61    1035 
   Size:         61     974
 
AvaI C'yCGr_G

Cuts at:      0     156     643    1035 
   Size:        156     487     392

  Fragments arranged by size: 

                 487     392     156
 
BanI G'GyrC_C

Cuts at:      0     363     940    1035 
   Size:        363     577      95

  Fragments arranged by size: 

                 577     363      95
 
BanII G_rGCy'C

Cuts at:      0     240     254     347     902    1035 
   Size:        240      14      93     555     133

  Fragments arranged by size: 

                 555     240     133      93      14
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     679    1035 
   Size:        679     356
 
BglI GCCn_nnn'nGGC

Cuts at:      0     503    1035 
   Size:        503     532
 
BlpI GC'TnA_GC

Cuts at:      0     818    1035 
   Size:        818     217
 
Bme1580I G_kGCm'C

Cuts at:      0     149     240     254     902    1035 
   Size:        149      91      14     648     133

  Fragments arranged by size: 

                 648     149     133      91      14
 
BmgBI CAC'GTC

Cuts at:      0     168     438    1035 
   Size:        168     270     597

  Fragments arranged by size: 

                 597     270     168
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     869    1035 
   Size:        869     166
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     279     574    1035 
   Size:        279     295     461

  Fragments arranged by size: 

                 461     295     279
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     448    1035 
   Size:        448     587
 
BsaAI yAC'GTr

Cuts at:      0     592    1035 
   Size:        592     443
 
BsaHI Gr'CG_yC

Cuts at:      0      68    1035 
   Size:         68     967
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     777     807    1035 
   Size:        777      30     228

  Fragments arranged by size: 

                 777     228      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1025    1035 
   Size:       1025      10
 
BseYI C'CCAG_C

Cuts at:      0     908     992    1035 
   Size:        908      84      43

  Fragments arranged by size: 

                 908      84      43
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     295     460     901    1035 
   Size:        214      81     165     441     134

  Fragments arranged by size: 

                 441     214     165     134      81
 
BsiEI CG_ry'CG

Cuts at:      0      10    1035 
   Size:         10    1025
 
BsiHKAI G_wGCw'C

Cuts at:      0     149    1035 
   Size:        149     886
 
BsmI GAATG_Cn'

Cuts at:      0    1004    1035 
   Size:       1004      31
 
Bsp1286I G_dGCh'C

Cuts at:      0     149     240     254     347     902    1035 
   Size:        149      91      14      93     555     133

  Fragments arranged by size: 

                 555     149     133      93      91      14
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     209     923    1035 
   Size:        209     714     112

  Fragments arranged by size: 

                 714     209     112
 
BsrBI CCG'CTC

Cuts at:      0     110     558    1035 
   Size:        110     448     477

  Fragments arranged by size: 

                 477     448     110
 
BssHII G'CGCG_C

Cuts at:      0      25    1035 
   Size:         25    1010
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     303    1035 
   Size:        303     732
 
BstYI r'GATC_y

Cuts at:      0     152     638    1035 
   Size:        152     486     397

  Fragments arranged by size: 

                 486     397     152
 
BtsI GCAGTG_nn'

Cuts at:      0     259     849    1035 
   Size:        259     590     186

  Fragments arranged by size: 

                 590     259     186
 
EaeI y'GGCC_r

Cuts at:      0       7     242     355     379    1035 
   Size:          7     235     113      24     656

  Fragments arranged by size: 

                 656     235     113      24       7
 
EagI C'GGCC_G

Cuts at:      0       7    1035 
   Size:          7    1028
 
EarI CTCTTCn'nnn_

Cuts at:      0      49    1035 
   Size:         49     986
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     403    1035 
   Size:        403     632
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     279     574    1011    1035 
   Size:        279     295     437      24

  Fragments arranged by size: 

                 437     295     279      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0     237     251     549     790     805     898     899     912 
   Size:        237      14     298     241      15      93       1      13

Cuts at:    912    1035 
   Size:        123

  Fragments arranged by size: 

                 298     241     237     123      93      15      14      13
                   1
 
FspI TGC'GCA

Cuts at:      0     373    1035 
   Size:        373     662
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     777     809    1035 
   Size:        777      32     226

  Fragments arranged by size: 

                 777     226      32
 
KpnI G_GTAC'C

Cuts at:      0     944    1035 
   Size:        944      91
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     102     341    1035 
   Size:        102     239     694

  Fragments arranged by size: 

                 694     239     102
 
MscI TGG'CCA

Cuts at:      0     244    1035 
   Size:        244     791
 
MslI CAynn'nnrTG

Cuts at:      0     436     482     529    1035 
   Size:        436      46      47     506

  Fragments arranged by size: 

                 506     436      47      46
 
MspA1I CmG'CkG

Cuts at:      0     248     299     330    1035 
   Size:        248      51      31     705

  Fragments arranged by size: 

                 705     248      51      31
 
PasI CC'CwG_GG

Cuts at:      0     501     546    1035 
   Size:        501      45     489

  Fragments arranged by size: 

                 501     489      45
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     552    1035 
   Size:        552     483
 
PfoI T'CCnGG_A

Cuts at:      0     116     564    1035 
   Size:        116     448     471

  Fragments arranged by size: 

                 471     448     116
 
PpuMI rG'GwC_Cy

Cuts at:      0     790     912    1035 
   Size:        790     122     123

  Fragments arranged by size: 

                 790     123     122
 
PspOMI G'GGCC_C

Cuts at:      0     236     250     898    1035 
   Size:        236      14     648     137

  Fragments arranged by size: 

                 648     236     137      14
 
PspXI vC'TCGA_Gb

Cuts at:      0     643    1035 
   Size:        643     392
 
PstI C_TGCA'G

Cuts at:      0     329     804    1035 
   Size:        329     475     231

  Fragments arranged by size: 

                 475     329     231
 
SapI GCTCTTCn'nnn_

Cuts at:      0      49    1035 
   Size:         49     986
 
SexAI A'CCwGG_T

Cuts at:      0     848    1035 
   Size:        848     187
 
SfcI C'TryA_G

Cuts at:      0     325     800    1035 
   Size:        325     475     235

  Fragments arranged by size: 

                 475     325     235
 
SmaI CCC'GGG

Cuts at:      0     158    1035 
   Size:        158     877
 
SmlI C'TyrA_G

Cuts at:      0     643    1035 
   Size:        643     392
 
StyI C'CwwG_G

Cuts at:      0     677    1035 
   Size:        677     358
 
TatI w'GTAC_w

Cuts at:      0     610    1035 
   Size:        610     425
 
Tth111I GACn'n_nGTC

Cuts at:      0     850    1035 
   Size:        850     185
 
XhoI C'TCGA_G

Cuts at:      0     643    1035 
   Size:        643     392
 
XmaI C'CCGG_G

Cuts at:      0     156    1035 
   Size:        156     879
 

 Enzymes that do cut:

   Acc65I     AcuI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AvaI
     BanI    BanII     BbsI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BpmI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI   BssHII    BstXI
    BstYI     BtsI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I
     FspI    Hin4I     KpnI     MmeI     MscI     MslI   MspA1I     PasI
    PflMI     PfoI    PpuMI   PspOMI    PspXI     PstI     SapI    SexAI
     SfcI     SmaI     SmlI     StyI     TatI  Tth111I     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BmtI     BplI   Bpu10I    BpuEI    BsaBI    BsaWI    BsiWI    BsmBI
    BspEI    BspHI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     MfeI     MluI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PmlI     PpiI    PshAI
     PsiI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TstI
     XbaI     XcmI     XmnI     ZraI
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