Restriction map for pF1KB7984
 (Circular) (Six-base) MAPSORT of: pF1KB7984.seq  Check: 3966  from: 1  to: 4085



pF1KB7984  4085 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AccI GT'mk_AC

Cuts at:   1123    1123 
   Size:       4085
 
Acc65I G'GTAC_C

Cuts at:   1101    1101 
   Size:       4085
 
AclI AA'CG_TT

Cuts at:   3925    3925 
   Size:       4085
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1910    2342    3089    3357    1910 
   Size:        432     747     268    2638

  Fragments arranged by size: 

                2638     747     432     268
 
AflIII A'CryG_T

Cuts at:    461     635     953     955    1437    2541    3518     461 
   Size:        174     318       2     482    1104     977    1028

  Fragments arranged by size: 

                1104    1028     977     482     318     174       2
 
AgeI A'CCGG_T

Cuts at:   2473    2473 
   Size:       4085
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1021    1053    1021 
   Size:         32    4053
 
AlwNI CAG_nnn'CTG

Cuts at:    426    1390    2957     426 
   Size:        964    1567    1554

  Fragments arranged by size: 

                1567    1554     964
 
ApaLI G'TGCA_C

Cuts at:    274    2855     274 
   Size:       2581    1504
 
ApoI r'AATT_y

Cuts at:    888    1089    3438    3694     888 
   Size:        201    2349     256    1279

  Fragments arranged by size: 

                2349    1279     256     201
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4085
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4085
 
AvaI C'yCGr_G

Cuts at:    509     831     963    1105     509 
   Size:        322     132     142    3489

  Fragments arranged by size: 

                3489     322     142     132
 
BamHI G'GATC_C

Cuts at:   1110    1110 
   Size:       4085
 
BanI G'GyrC_C

Cuts at:    352     807     897    1101    1515    1763    1798     352 
   Size:        455      90     204     414     248      35    2639

  Fragments arranged by size: 

                2639     455     414     248     204      90      35
 
BanII G_rGCy'C

Cuts at:    617     838    1099    2129     617 
   Size:        221     261    1030    2573

  Fragments arranged by size: 

                2573    1030     261     221
 
BbeI G_GCGC'C

Cuts at:    356    1519    1767     356 
   Size:       1163     248    2674

  Fragments arranged by size: 

                2674    1163     248
 
BbvCI CC'TCA_GC

Cuts at:    813     813 
   Size:       4085
 
BciVI GTATCCnnnnn_n'

Cuts at:   1983    2744    4085    1983 
   Size:        761    1341    1983

  Fragments arranged by size: 

                1983    1341     761
 
BclI T'GATC_A

Cuts at:   1605    1605 
   Size:       4085
 
BglII A'GATC_T

Cuts at:   1600    3637    1600 
   Size:       2037    2048
 
BlpI GC'TnA_GC

Cuts at:   1197    1197 
   Size:       4085
 
Bme1580I G_kGCm'C

Cuts at:    278     812    1710    1803    2859     278 
   Size:        534     898      93    1056    1504

  Fragments arranged by size: 

                1504    1056     898     534      93
 
BmgBI CAC'GTC

Cuts at:    348     348 
   Size:       4085
 
BmrI ACTGGGnnnn_n'

Cuts at:    672    1297    1378    1713    3857     672 
   Size:        625      81     335    2144     900

  Fragments arranged by size: 

                2144     900     625     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2452    2452 
   Size:       4085
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    279     289     403     448     822     987     279 
   Size:         10     114      45     374     165    3377

  Fragments arranged by size: 

                3377     374     165     114      45      10
 
Bpu10I CC'TnA_GC

Cuts at:    813     813 
   Size:       4085
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1261    2140    2632    2930    1261 
   Size:        879     492     298    2416

  Fragments arranged by size: 

                2416     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3789    3789 
   Size:       4085
 
BsaAI yAC'GTr

Cuts at:    956    1438     956 
   Size:        482    3603
 
BsaBI GATnn'nnATC

Cuts at:      6    1599    3636       6 
   Size:       1593    2037     455

  Fragments arranged by size: 

                2037    1593     455
 
BsaHI Gr'CG_yC

Cuts at:    353     827     904    1516    1764    2466    3962     353 
   Size:        474      77     612     248     702    1496     476

  Fragments arranged by size: 

                1496     702     612     476     474     248      77
 
BsaWI w'CCGG_w

Cuts at:   1274    1795    2473    2747    2894    3305    1274 
   Size:        521     678     274     147     411    2054

  Fragments arranged by size: 

                2054     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    268     298    1021    1051     268 
   Size:         30     723      30    3302

  Fragments arranged by size: 

                3302     723      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    165     243     923     165 
   Size:         78     680    3327

  Fragments arranged by size: 

                3327     680      78
 
BseYI C'CCAG_C

Cuts at:    197    1510    2268    2845    3341     197 
   Size:       1313     758     577     496     941

  Fragments arranged by size: 

                1313     941     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1045    1045 
   Size:       4085
 
BsiEI CG_ry'CG

Cuts at:    104     170     244    1627    1673    2881     104 
   Size:         66      74    1383      46    1208    1308

  Fragments arranged by size: 

                1383    1308    1208      74      66      46
 
BsiHKAI G_wGCw'C

Cuts at:    143     278    1099    1877    2067    2859     143 
   Size:        135     821     778     190     792    1369

  Fragments arranged by size: 

                1369     821     792     778     190     135
 
BsmBI CGTCTCn'nnnn_

Cuts at:    177     177 
   Size:       4085
 
Bsp1286I G_dGCh'C

Cuts at:    143     278     617     812     838    1099    1710    1803    1877 
   Size:        135     339     195      26     261     611      93      74

Cuts at:   1877    2067    2129    2859     143 
   Size:        190      62     730    1369

  Fragments arranged by size: 

                1369     730     611     339     261     195     190     135
                  93      74      62      26
 
BspEI T'CCGG_A

Cuts at:   3305    3305 
   Size:       4085
 
BspHI T'CATG_A

Cuts at:   3261    3261 
   Size:       4085
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1135    1349    1651    2032    1135 
   Size:        214     302     381    3188

  Fragments arranged by size: 

                3188     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2377    2431    3915    4080      50 
   Size:       2327      54    1484     165      55

  Fragments arranged by size: 

                2327    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1997    1997 
   Size:       4085
 
BsrFI r'CCGG_y

Cuts at:   1070    2083    2264    2473    1070 
   Size:       1013     181     209    2682

  Fragments arranged by size: 

                2682    1013     209     181
 
BssSI C'ACGA_G

Cuts at:   2356    2714    2356 
   Size:        358    3727
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1462    1462 
   Size:       4085
 
BstBI TT'CG_AA

Cuts at:   2446    2446 
   Size:       4085
 
BstYI r'GATC_y

Cuts at:      1    1110    1577    1600    1935    2181    3193    3279    3291 
   Size:       1109     467      23     335     246    1012      86      12

Cuts at:   3291    3637       1 
   Size:        346     449

  Fragments arranged by size: 

                1109    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    209     893    1076     209 
   Size:        684     183    3218

  Fragments arranged by size: 

                3218     684     183
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2035    2216    2379    2035 
   Size:        181     163    3741

  Fragments arranged by size: 

                3741     181     163
 
BtsI GCAGTG_nn'

Cuts at:    824    1070    1328     824 
   Size:        246     258    3581

  Fragments arranged by size: 

                3581     258     246
 
DraI TTT'AAA

Cuts at:   1084    1084 
   Size:       4085
 
DrdI GACnn_nn'nnGTC

Cuts at:    162    1791    2649     162 
   Size:       1629     858    1598

  Fragments arranged by size: 

                1629    1598     858
 
EaeI y'GGCC_r

Cuts at:    167     971    1670    1844    2235    2262    3607     167 
   Size:        804     699     174     391      27    1345     645

  Fragments arranged by size: 

                1345     804     699     645     391     174      27
 
EagI C'GGCC_G

Cuts at:    167    1670     167 
   Size:       1503    2582
 
EarI CTCTTCn'nnn_

Cuts at:   2108    2318    2108 
   Size:        210    3875
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    675    2293    2603    2749    3895     675 
   Size:       1618     310     146    1146     865

  Fragments arranged by size: 

                1618    1146     865     310     146
 
EcoICRI GAG'CTC

Cuts at:   1097    1097 
   Size:       4085
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    279     289     403     448     822     987    1910    2342    3089 
   Size:         10     114      45     374     165     923     432     747

Cuts at:   3089    3357     279 
   Size:        268    1007

  Fragments arranged by size: 

                1007     923     747     432     374     268     165     114
                  45      10
 
EcoO109I rG'GnC_Cy

Cuts at:    360     675    1224     360 
   Size:        315     549    3221

  Fragments arranged by size: 

                3221     549     315
 
EcoRI G'AATT_C

Cuts at:    888    1089     888 
   Size:        201    3884
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    145     609    2104     145 
   Size:        464    1495    2126

  Fragments arranged by size: 

                2126    1495     464
 
FspI TGC'GCA

Cuts at:   1866    1866 
   Size:       4085
 
HaeII r_GCGC'y

Cuts at:    356    1519    1767    2789    3768     356 
   Size:       1163     248    1022     979     673

  Fragments arranged by size: 

                1163    1022     979     673     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    349     381    1927    1957    1959    1989     349 
   Size:         32    1546      30       2      30    2445

  Fragments arranged by size: 

                2445    1546      32      30      30       2
 
HincII GTy'rAC

Cuts at:    735    1124     735 
   Size:        389    3696
 
KasI G'GCGC_C

Cuts at:    352    1515    1763     352 
   Size:       1163     248    2674

  Fragments arranged by size: 

                2674    1163     248
 
KpnI G_GTAC'C

Cuts at:   1105    1105 
   Size:       4085
 
MluI A'CGCG_T

Cuts at:   3518    3518 
   Size:       4085
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    311    1064    2756    2940     311 
   Size:        753    1692     184    1456

  Fragments arranged by size: 

                1692    1456     753     184
 
MscI TGG'CCA

Cuts at:    973    1846     973 
   Size:        873    3212
 
MslI CAynn'nnrTG

Cuts at:    790    2074    2201     790 
   Size:       1284     127    2674

  Fragments arranged by size: 

                2674    1284     127
 
MspA1I CmG'CkG

Cuts at:    151     393     895    1196    1510    1870    2883    3128     151 
   Size:        242     502     301     314     360    1013     245    1108

  Fragments arranged by size: 

                1108    1013     502     360     314     301     245     242
            
 
NaeI GCC'GGC

Cuts at:   2266    2266 
   Size:       4085
 
NarI GG'CG_CC

Cuts at:    353    1516    1764     353 
   Size:       1163     248    2674

  Fragments arranged by size: 

                2674    1163     248
 
NcoI C'CATG_G

Cuts at:    209     209 
   Size:       4085
 
NgoMIV G'CCGG_C

Cuts at:   2264    2264 
   Size:       4085
 
NspI r_CATG'y

Cuts at:    465     639     759     795    1138    1638    2545     465 
   Size:        174     120      36     343     500     907    2005

  Fragments arranged by size: 

                2005     907     500     343     174     120      36
 
PciI A'CATG_T

Cuts at:    461     635    2541     461 
   Size:        174    1906    2005

  Fragments arranged by size: 

                2005    1906     174
 
PflMI CCAn_nnn'nTGG

Cuts at:    426     426 
   Size:       4085
 
PfoI T'CCnGG_A

Cuts at:    419     419 
   Size:       4085
 
PmeI GTTT'AAAC

Cuts at:   1084    1084 
   Size:       4085
 
PmlI CAC'GTG

Cuts at:    956     956 
   Size:       4085
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1021    1053    3224    3256    1021 
   Size:         32    2171      32    1850

  Fragments arranged by size: 

                2171    1850      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    360     360 
   Size:       4085
 
PshAI GACnn'nnGTC

Cuts at:    826     826 
   Size:       4085
 
PspXI vC'TCGA_Gb

Cuts at:    963     963 
   Size:       4085
 
PstI C_TGCA'G

Cuts at:   1132    1817    1132 
   Size:        685    3400
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4085
 
PvuII CAG'CTG

Cuts at:   1510    1870    1510 
   Size:        360    3725
 
RsrII CG'GwC_CG

Cuts at:   2280    2280 
   Size:       4085
 
SacI G_AGCT'C

Cuts at:   1099    1099 
   Size:       4085
 
SacII CC_GC'GG

Cuts at:    896     896 
   Size:       4085
 
SalI G'TCGA_C

Cuts at:   1122    1122 
   Size:       4085
 
SapI GCTCTTCn'nnn_

Cuts at:   2108    2318    2108 
   Size:        210    3875
 
SbfI CC_TGCA'GG

Cuts at:   1132    1132 
   Size:       4085
 
ScaI AGT'ACT

Cuts at:   3602    3602 
   Size:       4085
 
SfcI C'TryA_G

Cuts at:     33    1128    1813    2806    2997      33 
   Size:       1095     685     993     191    1121

  Fragments arranged by size: 

                1121    1095     993     685     191
 
SfoI GGC'GCC

Cuts at:    354    1517    1765     354 
   Size:       1163     248    2674

  Fragments arranged by size: 

                2674    1163     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4085
 
SmaI CCC'GGG

Cuts at:    833    1107     833 
   Size:        274    3811
 
SmlI C'TyrA_G

Cuts at:    963    1240    2155    2647    2909     963 
   Size:        277     915     492     262    2139

  Fragments arranged by size: 

                2139     915     492     277     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4085
 
SphI G_CATG'C

Cuts at:   1138    1638    1138 
   Size:        500    3585
 
StyI C'CwwG_G

Cuts at:    209     671    1219    1573     209 
   Size:        462     548     354    2721

  Fragments arranged by size: 

                2721     548     462     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    783    1668    1801    2260    2469     783 
   Size:        885     133     459     209    2399

  Fragments arranged by size: 

                2399     885     459     209     133
 
TatI w'GTAC_w

Cuts at:   3600    3600 
   Size:       4085
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    268     300    3602    3634     268 
   Size:         32    3302      32     719

  Fragments arranged by size: 

                3302     719      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1882    1882 
   Size:       4085
 
XbaI T'CTAG_A

Cuts at:   1116    1116 
   Size:       4085
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    264    1580     264 
   Size:       1316    2769
 
XhoI C'TCGA_G

Cuts at:    963     963 
   Size:       4085
 
XmaI C'CCGG_G

Cuts at:    831    1105     831 
   Size:        274    3811
 
XmnI GAAnn'nnTTC

Cuts at:    592     592 
   Size:       4085
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AloI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI    PspXI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     ApaI
     AscI    AvrII     BaeI     BbsI     BcgI    BfrBI     BglI     BmtI
    BsiWI     BsmI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PsiI   PspOMI     PsrI    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     ZraI
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