Restriction map for pF1KB7984
 (Linear) (Six-base) MAPSORT of: KIBB7984.seq  Check: 3671  from: 1  to: 972



KIBB7984.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:25  ..

AflIII A'CryG_T

Cuts at:      0     353     527     845     847     972 
   Size:        353     174     318       2     125

  Fragments arranged by size: 

                 353     318     174     125       2
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     913     945     972 
   Size:        913      32      27

  Fragments arranged by size: 

                 913      32      27
 
AlwNI CAG_nnn'CTG

Cuts at:      0     318     972 
   Size:        318     654
 
ApaLI G'TGCA_C

Cuts at:      0     166     972 
   Size:        166     806
 
ApoI r'AATT_y

Cuts at:      0     780     972 
   Size:        780     192
 
AvaI C'yCGr_G

Cuts at:      0     401     723     855     972 
   Size:        401     322     132     117

  Fragments arranged by size: 

                 401     322     132     117
 
BanI G'GyrC_C

Cuts at:      0     244     699     789     972 
   Size:        244     455      90     183

  Fragments arranged by size: 

                 455     244     183      90
 
BanII G_rGCy'C

Cuts at:      0     509     730     972 
   Size:        509     221     242

  Fragments arranged by size: 

                 509     242     221
 
BbeI G_GCGC'C

Cuts at:      0     248     972 
   Size:        248     724
 
BbvCI CC'TCA_GC

Cuts at:      0     705     972 
   Size:        705     267
 
Bme1580I G_kGCm'C

Cuts at:      0     170     704     972 
   Size:        170     534     268

  Fragments arranged by size: 

                 534     268     170
 
BmgBI CAC'GTC

Cuts at:      0     240     972 
   Size:        240     732
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     564     972 
   Size:        564     408
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     181     295     340     714     879     972 
   Size:        171      10     114      45     374     165      93

  Fragments arranged by size: 

                 374     171     165     114      93      45      10
 
Bpu10I CC'TnA_GC

Cuts at:      0     705     972 
   Size:        705     267
 
BsaAI yAC'GTr

Cuts at:      0     848     972 
   Size:        848     124
 
BsaHI Gr'CG_yC

Cuts at:      0     245     719     796     972 
   Size:        245     474      77     176

  Fragments arranged by size: 

                 474     245     176      77
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     160     190     913     943     972 
   Size:        160      30     723      30      29

  Fragments arranged by size: 

                 723     160      30      30      29
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      57     135     815     972 
   Size:         57      78     680     157

  Fragments arranged by size: 

                 680     157      78      57
 
BseYI C'CCAG_C

Cuts at:      0      89     972 
   Size:         89     883
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     937     972 
   Size:        937      35
 
BsiEI CG_ry'CG

Cuts at:      0      62     136     972 
   Size:         62      74     836

  Fragments arranged by size: 

                 836      74      62
 
BsiHKAI G_wGCw'C

Cuts at:      0      35     170     972 
   Size:         35     135     802

  Fragments arranged by size: 

                 802     135      35
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      69     972 
   Size:         69     903
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     170     509     704     730     972 
   Size:         35     135     339     195      26     242

  Fragments arranged by size: 

                 339     242     195     135      35      26
 
BsrFI r'CCGG_y

Cuts at:      0     962     972 
   Size:        962      10
 
BtgI C'CryG_G

Cuts at:      0     101     785     972 
   Size:        101     684     187

  Fragments arranged by size: 

                 684     187     101
 
BtsI GCAGTG_nn'

Cuts at:      0     716     962     972 
   Size:        716     246      10

  Fragments arranged by size: 

                 716     246      10
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      54     972 
   Size:         54     918
 
EaeI y'GGCC_r

Cuts at:      0      59     863     972 
   Size:         59     804     109

  Fragments arranged by size: 

                 804     109      59
 
EagI C'GGCC_G

Cuts at:      0      59     972 
   Size:         59     913
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     567     972 
   Size:        567     405
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     171     181     295     340     714     879     972 
   Size:        171      10     114      45     374     165      93

  Fragments arranged by size: 

                 374     171     165     114      93      45      10
 
EcoO109I rG'GnC_Cy

Cuts at:      0     252     567     972 
   Size:        252     315     405

  Fragments arranged by size: 

                 405     315     252
 
EcoRI G'AATT_C

Cuts at:      0     780     972 
   Size:        780     192
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      37     501     972 
   Size:         37     464     471

  Fragments arranged by size: 

                 471     464      37
 
HaeII r_GCGC'y

Cuts at:      0     248     972 
   Size:        248     724
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     241     273     972 
   Size:        241      32     699

  Fragments arranged by size: 

                 699     241      32
 
HincII GTy'rAC

Cuts at:      0     627     972 
   Size:        627     345
 
KasI G'GCGC_C

Cuts at:      0     244     972 
   Size:        244     728
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     203     956     972 
   Size:        203     753      16

  Fragments arranged by size: 

                 753     203      16
 
MscI TGG'CCA

Cuts at:      0     865     972 
   Size:        865     107
 
MslI CAynn'nnrTG

Cuts at:      0     682     972 
   Size:        682     290
 
MspA1I CmG'CkG

Cuts at:      0      43     285     787     972 
   Size:         43     242     502     185

  Fragments arranged by size: 

                 502     242     185      43
 
NarI GG'CG_CC

Cuts at:      0     245     972 
   Size:        245     727
 
NcoI C'CATG_G

Cuts at:      0     101     972 
   Size:        101     871
 
NspI r_CATG'y

Cuts at:      0     357     531     651     687     972 
   Size:        357     174     120      36     285

  Fragments arranged by size: 

                 357     285     174     120      36
 
PciI A'CATG_T

Cuts at:      0     353     527     972 
   Size:        353     174     445

  Fragments arranged by size: 

                 445     353     174
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     318     972 
   Size:        318     654
 
PfoI T'CCnGG_A

Cuts at:      0     311     972 
   Size:        311     661
 
PmlI CAC'GTG

Cuts at:      0     848     972 
   Size:        848     124
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     913     945     972 
   Size:        913      32      27

  Fragments arranged by size: 

                 913      32      27
 
PpuMI rG'GwC_Cy

Cuts at:      0     252     972 
   Size:        252     720
 
PshAI GACnn'nnGTC

Cuts at:      0     718     972 
   Size:        718     254
 
PspXI vC'TCGA_Gb

Cuts at:      0     855     972 
   Size:        855     117
 
SacII CC_GC'GG

Cuts at:      0     788     972 
   Size:        788     184
 
SfoI GGC'GCC

Cuts at:      0     246     972 
   Size:        246     726
 
SmaI CCC'GGG

Cuts at:      0     725     972 
   Size:        725     247
 
SmlI C'TyrA_G

Cuts at:      0     855     972 
   Size:        855     117
 
StyI C'CwwG_G

Cuts at:      0     101     563     972 
   Size:        101     462     409

  Fragments arranged by size: 

                 462     409     101
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     675     972 
   Size:        675     297
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     160     192     972 
   Size:        160      32     780

  Fragments arranged by size: 

                 780     160      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     156     972 
   Size:        156     816
 
XhoI C'TCGA_G

Cuts at:      0     855     972 
   Size:        855     117
 
XmaI C'CCGG_G

Cuts at:      0     723     972 
   Size:        723     249
 
XmnI GAAnn'nnTTC

Cuts at:      0     484     972 
   Size:        484     488
 

 Enzymes that do cut:

   AflIII     AloI    AlwNI    ApaLI     ApoI     AvaI     BanI    BanII
     BbeI    BbvCI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BsaAI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI
 Bsp1286I    BsrFI     BtgI     BtsI     DrdI     EaeI     EagI     EciI
  Eco57MI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII     KasI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NspI     PciI
    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI    PspXI    SacII
     SfoI     SmaI     SmlI     StyI    TaqII     TstI     XcmI     XhoI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     ApaI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI     BmtI     BplI    BpuEI     BsaI    BsaBI
    BsaWI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     EarI
  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI  HindIII     HpaI
     KpnI     MfeI     MluI     NaeI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PmeI     PsiI   PspOMI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I
     XbaI     ZraI
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