Restriction map for pF1KB4740
 (Circular) (Six-base) MAPSORT of: pF1KB4740.seq  Check: 2377  from: 1  to: 5723



pF1KB4740  5723 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    885    2439    2626     885 
   Size:       1554     187    3982

  Fragments arranged by size: 

                3982    1554     187
 
AatII G_ACGT'C

Cuts at:    871     871 
   Size:       5723
 
AccI GT'mk_AC

Cuts at:   2761    2761 
   Size:       5723
 
Acc65I G'GTAC_C

Cuts at:   2506    2739    2506 
   Size:        233    5490
 
AclI AA'CG_TT

Cuts at:   5563    5563 
   Size:       5723
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    689     965    1248    2188    3548    3980    4333    4995     689 
   Size:        276     283     940    1360     432     353     662    1417

  Fragments arranged by size: 

                1417    1360     940     662     432     353     283     276
            
 
AflIII A'CryG_T

Cuts at:   1050    3075    4875    5156    1050 
   Size:       2025    1800     281    1617

  Fragments arranged by size: 

                2025    1800    1617     281
 
AgeI A'CCGG_T

Cuts at:   2503    2503 
   Size:       5723
 
AhdI GACnn_n'nnGTC

Cuts at:   2040    2040 
   Size:       5723
 
AlwNI CAG_nnn'CTG

Cuts at:    835     991    3028    4466     835 
   Size:        156    2037    1438    2092

  Fragments arranged by size: 

                2092    2037    1438     156
 
ApaI G_GGCC'C

Cuts at:   1324    1324 
   Size:       5723
 
ApaLI G'TGCA_C

Cuts at:   4561    4561 
   Size:       5723
 
ApoI r'AATT_y

Cuts at:   2727    5076    5332    2727 
   Size:       2349     256    3118

  Fragments arranged by size: 

                3118    2349     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       5723
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5723
 
AvaI C'yCGr_G

Cuts at:   1133    1536    2249    2743    1133 
   Size:        403     713     494    4113

  Fragments arranged by size: 

                4113     713     494     403
 
BamHI G'GATC_C

Cuts at:   1407    1527    2480    2748    1407 
   Size:        120     953     268    4382

  Fragments arranged by size: 

                4382     953     268     120
 
BanI G'GyrC_C

Cuts at:    397     641     758    2506    2739    3153    3401    3436     397 
   Size:        244     117    1748     233     414     248      35    2684

  Fragments arranged by size: 

                2684    1748     414     248     244     233     117      35
            
 
BanII G_rGCy'C

Cuts at:    295    1324    2737    3767     295 
   Size:       1029    1413    1030    2251

  Fragments arranged by size: 

                2251    1413    1030    1029
 
BbeI G_GCGC'C

Cuts at:   3157    3405    3157 
   Size:        248    5475
 
BbsI GAAGACnn'nnnn_

Cuts at:    277    1280     277 
   Size:       1003    4720
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1923    1957    1923 
   Size:         34    5689
 
BciVI GTATCCnnnnn_n'

Cuts at:   3621    4677    5723    3621 
   Size:       1056    1046    3621

  Fragments arranged by size: 

                3621    1056    1046
 
BclI T'GATC_A

Cuts at:   3243    3243 
   Size:       5723
 
BglI GCCn_nnn'nGGC

Cuts at:    791     940     791 
   Size:        149    5574
 
BglII A'GATC_T

Cuts at:   3238    5275    3238 
   Size:       2037    3686
 
BlpI GC'TnA_GC

Cuts at:   2835    2835 
   Size:       5723
 
Bme1580I G_kGCm'C

Cuts at:    576    1324    3348    3441    4565     576 
   Size:        748    2024      93    1124    1734

  Fragments arranged by size: 

                2024    1734    1124     748      93
 
BmgBI CAC'GTC

Cuts at:   1051    1051 
   Size:       5723
 
BmrI ACTGGGnnnn_n'

Cuts at:    251     284    1392    2152    2935    3016    3351    5495     251 
   Size:         33    1108     760     783      81     335    2144     479

  Fragments arranged by size: 

                2144    1108     783     760     479     335      81      33
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4090    4090 
   Size:       5723
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    384     594     614    1091    1228    2059    2131    2329    2362 
   Size:        210      20     477     137     831      72     198      33

Cuts at:   2362     384 
   Size:       3745

  Fragments arranged by size: 

                3745     831     477     210     198     137      72      33
                  20
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    399     909    2899    3778    4492    4790     399 
   Size:        510    1990     879     714     298    1332

  Fragments arranged by size: 

                1990    1332     879     714     510     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    256    2013    2689    5427     256 
   Size:       1757     676    2738     552

  Fragments arranged by size: 

                2738    1757     676     552
 
BsaAI yAC'GTr

Cuts at:    231    1203    1374    1650    3076     231 
   Size:        972     171     276    1426    2878

  Fragments arranged by size: 

                2878    1426     972     276     171
 
BsaBI GATnn'nnATC

Cuts at:      6    3237    5274       6 
   Size:       3231    2037     455

  Fragments arranged by size: 

                3231    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    868    3154    3402    4104    5600     868 
   Size:       2286     248     702    1496     991

  Fragments arranged by size: 

                2286    1496     991     702     248
 
BsaWI w'CCGG_w

Cuts at:   2503    2912    3433    4111    4522    4669    4943    2503 
   Size:        409     521     678     411     147     274    3283

  Fragments arranged by size: 

                3283     678     521     411     409     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    965     995    1873    1903     965 
   Size:         30     878      30    4785

  Fragments arranged by size: 

                4785     878      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1774    1881    2215    2707    1774 
   Size:        107     334     492    4790

  Fragments arranged by size: 

                4790     492     334     107
 
BseYI C'CCAG_C

Cuts at:    652     720     930     958     984    1112    1337    3148    3906 
   Size:         68     210      28      26     128     225    1811     758

Cuts at:   3906    4571    4979     652 
   Size:        665     408    1396

  Fragments arranged by size: 

                1811    1396     758     665     408     225     210     128
                  68      28      26
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    866     866 
   Size:       5723
 
BsiEI CG_ry'CG

Cuts at:    104    1193    3265    3311    4541     104 
   Size:       1089    2072      46    1230    1286

  Fragments arranged by size: 

                2072    1286    1230    1089      46
 
BsiHKAI G_wGCw'C

Cuts at:    295     750    1823    2398    2737    3515    3705    4565     295 
   Size:        455    1073     575     339     778     190     860    1453

  Fragments arranged by size: 

                1453    1073     860     778     575     455     339     190
            
 
Bsp1286I G_dGCh'C

Cuts at:    295     576     750    1324    1823    2398    2737    3348    3441 
   Size:        281     174     574     499     575     339     611      93

Cuts at:   3441    3515    3705    3767    4565     295 
   Size:         74     190      62     798    1453

  Fragments arranged by size: 

                1453     798     611     575     574     499     339     281
                 190     174      93      74      62
 
BspHI T'CATG_A

Cuts at:   1703    4155    1703 
   Size:       2452    3271
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    482     885    1130    1733    2439    2518    2626    2773    2987 
   Size:        403     245     603     706      79     108     147     214

Cuts at:   2987    3289    3670     482 
   Size:        302     381    2535

  Fragments arranged by size: 

                2535     706     603     403     381     302     245     214
                 147     108      79
 
BsrBI CCG'CTC

Cuts at:     50    1095    4015    4069    5553    5718      50 
   Size:       1045    2920      54    1484     165      55

  Fragments arranged by size: 

                2920    1484    1045     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   3635    3635 
   Size:       5723
 
BsrFI r'CCGG_y

Cuts at:    638     727     755     939    1733    2405    2503    3721    3902 
   Size:         89      28     184     794     672      98    1218     181

Cuts at:   3902     638 
   Size:       2459

  Fragments arranged by size: 

                2459    1218     794     672     184     181      98      89
                  28
 
BssSI C'ACGA_G

Cuts at:   1813    2161    3994    4702    1813 
   Size:        348    1833     708    2834

  Fragments arranged by size: 

                2834    1833     708     348
 
BstAPI GCAn_nnn'nTGC

Cuts at:    662    3100     662 
   Size:       2438    3285
 
BstBI TT'CG_AA

Cuts at:   1682    4084    1682 
   Size:       2402    3321
 
BstYI r'GATC_y

Cuts at:      1     676    1128    1407    1527    2432    2480    2634    2748 
   Size:        675     452     279     120     905      48     154     114

Cuts at:   2748    3215    3238    3573    3819    4125    4137    4223    5275 
   Size:        467      23     335     246     306      12      86    1052

Cuts at:   5275       1 
   Size:        449

  Fragments arranged by size: 

                1052     905     675     467     452     449     335     306
                 279     246     154     120     114      86      48      23
                  12
 
BtgI C'CryG_G

Cuts at:    107    1193    1946    1959     107 
   Size:       1086     753      13    3871

  Fragments arranged by size: 

                3871    1086     753      13
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1767    1926    3673    3854    4017    1767 
   Size:        159    1747     181     163    3473

  Fragments arranged by size: 

                3473    1747     181     163     159
 
BtsI GCAGTG_nn'

Cuts at:    576    1225    2966     576 
   Size:        649    1741    3333

  Fragments arranged by size: 

                3333    1741     649
 
DraI TTT'AAA

Cuts at:    356    2722     356 
   Size:       2366    3357
 
DraIII CAC_nnn'GTG

Cuts at:   1818    1818 
   Size:       5723
 
DrdI GACnn_nn'nnGTC

Cuts at:   3429    4773    3429 
   Size:       1344    4379
 
EaeI y'GGCC_r

Cuts at:    797    3308    3482    3873    3900    5245     797 
   Size:       2511     174     391      27    1345    1275

  Fragments arranged by size: 

                2511    1345    1275     391     174      27
 
EagI C'GGCC_G

Cuts at:   3308    3308 
   Size:       5723
 
EarI CTCTTCn'nnn_

Cuts at:   2207    2255    2386    2470    3746    3956    2207 
   Size:         48     131      84    1276     210    3974

  Fragments arranged by size: 

                3974    1276     210     131      84      48
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    524    3931    4673    4819    5533     524 
   Size:       3407     742     146     714     714

  Fragments arranged by size: 

                3407     742     714     714     146
 
EcoICRI GAG'CTC

Cuts at:    293    2735     293 
   Size:       2442    3281
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    384     594     614     689     965    1091    1228    1248    2059 
   Size:        210      20      75     276     126     137      20     811

Cuts at:   2059    2131    2188    2329    2362    3548    3980    4333    4995 
   Size:         72      57     141      33    1186     432     353     662

Cuts at:   4995     384 
   Size:       1112

  Fragments arranged by size: 

                1186    1112     811     662     432     353     276     210
                 141     137     126      75      72      57      33      20
                  20
 
EcoO109I rG'GnC_Cy

Cuts at:    999    1321    1764    2862     999 
   Size:        322     443    1098    3860

  Fragments arranged by size: 

                3860    1098     443     322
 
EcoRI G'AATT_C

Cuts at:   2727    2727 
   Size:       5723
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3742    3742 
   Size:       5723
 
FspI TGC'GCA

Cuts at:   1207    3504    1207 
   Size:       2297    3426
 
HaeII r_GCGC'y

Cuts at:   1954    3157    3405    4635    5406    1954 
   Size:       1203     248    1230     771    2271

  Fragments arranged by size: 

                2271    1230    1203     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    888     920    2026    2058    3565    3595    3597    3627     888 
   Size:         32    1106      32    1507      30       2      30    2984

  Fragments arranged by size: 

                2984    1507    1106      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    211     411    1065    2762     211 
   Size:        200     654    1697    3172

  Fragments arranged by size: 

                3172    1697     654     200
 
KasI G'GCGC_C

Cuts at:   3153    3401    3153 
   Size:        248    5475
 
KpnI G_GTAC'C

Cuts at:   2510    2743    2510 
   Size:        233    5490
 
MluI A'CGCG_T

Cuts at:   5156    5156 
   Size:       5723
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    443    1213    1719    4482    4666     443 
   Size:        770     506    2763     184    1500

  Fragments arranged by size: 

                2763    1500     770     506     184
 
MscI TGG'CCA

Cuts at:    799    3484     799 
   Size:       2685    3038
 
MslI CAynn'nnrTG

Cuts at:    698    1631    1708    1747    1945    3712    3839     698 
   Size:        933      77      39     198    1767     127    2582

  Fragments arranged by size: 

                2582    1767     933     198     127      77      39
 
MspA1I CmG'CkG

Cuts at:    462     594     988    1195    1233    2307    2664    2834    3148 
   Size:        132     394     207      38    1074     357     170     314

Cuts at:   3148    3508    4292    4537     462 
   Size:        360     784     245    1648

  Fragments arranged by size: 

                1648    1074     784     394     360     357     314     245
                 207     170     132      38
 
NaeI GCC'GGC

Cuts at:    757    1735    3904     757 
   Size:        978    2169    2576

  Fragments arranged by size: 

                2576    2169     978
 
NarI GG'CG_CC

Cuts at:   3154    3402    3154 
   Size:        248    5475
 
NcoI C'CATG_G

Cuts at:    107    1946    1959     107 
   Size:       1839      13    3871

  Fragments arranged by size: 

                3871    1839      13
 
NgoMIV G'CCGG_C

Cuts at:    755    1733    3902     755 
   Size:        978    2169    2576

  Fragments arranged by size: 

                2576    2169     978
 
NspI r_CATG'y

Cuts at:    459    2364    2776    3276    4879     459 
   Size:       1905     412     500    1603    1303

  Fragments arranged by size: 

                1905    1603    1303     500     412
 
PasI CC'CwG_GG

Cuts at:   1317    1419    1317 
   Size:        102    5621
 
PciI A'CATG_T

Cuts at:   4875    4875 
   Size:       5723
 
PflMI CCAn_nnn'nTGG

Cuts at:   1088    1178    1793    2075    2133    1088 
   Size:         90     615     282      58    4678

  Fragments arranged by size: 

                4678     615     282      90      58
 
PfoI T'CCnGG_A

Cuts at:    527     679    1430    2126    2279     527 
   Size:        152     751     696     153    3971

  Fragments arranged by size: 

                3971     751     696     153     152
 
PmeI GTTT'AAAC

Cuts at:   2722    2722 
   Size:       5723
 
PmlI CAC'GTG

Cuts at:   1650    1650 
   Size:       5723
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4169    4201    4169 
   Size:         32    5691
 
PspOMI G'GGCC_C

Cuts at:   1320    1320 
   Size:       5723
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   1594    1626    1594 
   Size:         32    5691
 
PstI C_TGCA'G

Cuts at:    479    1877    2495    2770    3455     479 
   Size:       1398     618     275     685    2747

  Fragments arranged by size: 

                2747    1398     685     618     275
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5723
 
PvuII CAG'CTG

Cuts at:    462     594    2664    3148    3508     462 
   Size:        132    2070     484     360    2677

  Fragments arranged by size: 

                2677    2070     484     360     132
 
RsrII CG'GwC_CG

Cuts at:   3918    3918 
   Size:       5723
 
SacI G_AGCT'C

Cuts at:    295    2737     295 
   Size:       2442    3281
 
SacII CC_GC'GG

Cuts at:   1196    1196 
   Size:       5723
 
SalI G'TCGA_C

Cuts at:   2760    2760 
   Size:       5723
 
SapI GCTCTTCn'nnn_

Cuts at:   2255    2386    3746    3956    2255 
   Size:        131    1360     210    4022

  Fragments arranged by size: 

                4022    1360     210     131
 
SbfI CC_TGCA'GG

Cuts at:   2770    2770 
   Size:       5723
 
ScaI AGT'ACT

Cuts at:   5240    5240 
   Size:       5723
 
SfcI C'TryA_G

Cuts at:     33     475    1873    2491    2766    3451    4419    4610      33 
   Size:        442    1398     618     275     685     968     191    1146

  Fragments arranged by size: 

                1398    1146     968     685     618     442     275     191
            
 
SfoI GGC'GCC

Cuts at:   3155    3403    3155 
   Size:        248    5475
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5723
 
SmaI CCC'GGG

Cuts at:   1538    2745    1538 
   Size:       1207    4516
 
SmlI C'TyrA_G

Cuts at:    378     888    2878    3793    4507    4769     378 
   Size:        510    1990     915     714     262    1332

  Fragments arranged by size: 

                1990    1332     915     714     510     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       5723
 
SphI G_CATG'C

Cuts at:   2364    2776    3276    2364 
   Size:        412     500    4811

  Fragments arranged by size: 

                4811     500     412
 
StyI C'CwwG_G

Cuts at:    107     194    1946    1959    2857    3211     107 
   Size:         87    1752      13     898     354    2619

  Fragments arranged by size: 

                2619    1752     898     354      87      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2713    3306    3439    3898    4107    2713 
   Size:        593     133     459     209    4329

  Fragments arranged by size: 

                4329     593     459     209     133
 
TatI w'GTAC_w

Cuts at:   2117    2216    5238    2117 
   Size:         99    3022    2602

  Fragments arranged by size: 

                3022    2602      99
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    965     997    5240    5272     965 
   Size:         32    4243      32    1416

  Fragments arranged by size: 

                4243    1416      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   3520    3520 
   Size:       5723
 
XbaI T'CTAG_A

Cuts at:   2754    2754 
   Size:       5723
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2277    3218    2277 
   Size:        941    4782
 
XmaI C'CCGG_G

Cuts at:   1536    2743    1536 
   Size:       1207    4516
 
ZraI GAC'GTC

Cuts at:    869     869 
   Size:       5723
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AhdI    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI
    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI   PspOMI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AleI     AlfI     AloI     AscI    AvrII     BaeI
    BbvCI    BfrBI     BmtI   Bpu10I    BsiWI     BsmI    BsmBI    BspEI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI    PpuMI    PshAI     PsiI
    PspXI    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     StuI     SwaI     XhoI     XmnI
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