Restriction map for pF1KB6437
 (Linear) (Six-base) MAPSORT of: KIBB6437.seq  Check: 5911  from: 1  to: 939



KIBB6437.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AccI GT'mk_AC

Cuts at:      0     341     939 
   Size:        341     598
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     589     939 
   Size:        589     350
 
ApaLI G'TGCA_C

Cuts at:      0     650     939 
   Size:        650     289
 
ApoI r'AATT_y

Cuts at:      0     114     157     323     444     939 
   Size:        114      43     166     121     495

  Fragments arranged by size: 

                 495     166     121     114      43
 
AvrII C'CTAG_G

Cuts at:      0     870     939 
   Size:        870      69
 
BanII G_rGCy'C

Cuts at:      0      33     939 
   Size:         33     906
 
BglI GCCn_nnn'nGGC

Cuts at:      0      74     939 
   Size:         74     865
 
Bme1580I G_kGCm'C

Cuts at:      0     654     939 
   Size:        654     285
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     502     939 
   Size:        502     437
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     939 
   Size:        283     656
 
Bpu10I CC'TnA_GC

Cuts at:      0     134     473     939 
   Size:        134     339     466

  Fragments arranged by size: 

                 466     339     134
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     426     939 
   Size:        426     513
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     704     734     939 
   Size:        704      30     205

  Fragments arranged by size: 

                 704     205      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     542     939 
   Size:        542     397
 
BseYI C'CCAG_C

Cuts at:      0      32      55     939 
   Size:         32      23     884

  Fragments arranged by size: 

                 884      32      23
 
BsiHKAI G_wGCw'C

Cuts at:      0     654     939 
   Size:        654     285
 
BsmI GAATG_Cn'

Cuts at:      0     298     939 
   Size:        298     641
 
Bsp1286I G_dGCh'C

Cuts at:      0      33     654     939 
   Size:         33     621     285

  Fragments arranged by size: 

                 621     285      33
 
BsrGI T'GTAC_A

Cuts at:      0     765     939 
   Size:        765     174
 
BstYI r'GATC_y

Cuts at:      0     855     939 
   Size:        855      84
 
BtgI C'CryG_G

Cuts at:      0     146     939 
   Size:        146     793
 
DraI TTT'AAA

Cuts at:      0     285     939 
   Size:        285     654
 
EarI CTCTTCn'nnn_

Cuts at:      0      41     939 
   Size:         41     898
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     589     939 
   Size:        283     306     350

  Fragments arranged by size: 

                 350     306     283
 
EcoRI G'AATT_C

Cuts at:      0     323     939 
   Size:        323     616
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     816     939 
   Size:        816     123
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     256     288     474     506     939 
   Size:        256      32     186      32     433

  Fragments arranged by size: 

                 433     256     186      32      32
 
HincII GTy'rAC

Cuts at:      0     252     386     939 
   Size:        252     134     553

  Fragments arranged by size: 

                 553     252     134
 
HpaI GTT'AAC

Cuts at:      0     252     939 
   Size:        252     687
 
MfeI C'AATT_G

Cuts at:      0     824     939 
   Size:        824     115
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     844     939 
   Size:        844      95
 
MslI CAynn'nnrTG

Cuts at:      0     151     533     939 
   Size:        151     382     406

  Fragments arranged by size: 

                 406     382     151
 
NcoI C'CATG_G

Cuts at:      0     146     939 
   Size:        146     793
 
NspI r_CATG'y

Cuts at:      0      81     340     939 
   Size:         81     259     599

  Fragments arranged by size: 

                 599     259      81
 
PfoI T'CCnGG_A

Cuts at:      0     318     939 
   Size:        318     621
 
PstI C_TGCA'G

Cuts at:      0     570     939 
   Size:        570     369
 
SfcI C'TryA_G

Cuts at:      0     566     666     863     876     939 
   Size:        566     100     197      13      63

  Fragments arranged by size: 

                 566     197     100      63      13
 
SphI G_CATG'C

Cuts at:      0      81     939 
   Size:         81     858
 
SspI AAT'ATT

Cuts at:      0     237     939 
   Size:        237     702
 
StyI C'CwwG_G

Cuts at:      0     146     870     939 
   Size:        146     724      69

  Fragments arranged by size: 

                 724     146      69
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     387     939 
   Size:        387     552
 
TatI w'GTAC_w

Cuts at:      0     765     939 
   Size:        765     174
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     764     796     939 
   Size:        764      32     143

  Fragments arranged by size: 

                 764     143      32
 

 Enzymes that do cut:

     AccI     AcuI    ApaLI     ApoI    AvrII    BanII     BglI Bme1580I
     BplI     BpmI   Bpu10I     BsaI    BsaXI    BseRI    BseYI  BsiHKAI
     BsmI Bsp1286I    BsrGI    BstYI     BtgI     DraI     EarI  Eco57MI
    EcoRI     FalI    Hin4I   HincII     HpaI     MfeI     MmeI     MslI
     NcoI     NspI     PfoI     PstI     SfcI     SphI     SspI     StyI
    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     KasI     KpnI     MluI     MscI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     StuI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com