Restriction map for pF1KB3085
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BanII       BbrPI       BbvCI       
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaI        BsaWI       BseAI       BseRI       BsiEI       
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HgaI        HincII      HindII      HindIII     
HpaI        HphI        KpnI        KspI        MaeI        
MfeI        MluI        MroI        MseI        MslI        
MunI        NcoI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        TaqI        Tru9I       Tth111I     
TthHB8I     XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AcyI           58  GGCGCC               G[GA]^CG[CT]C
AluI           20  AGCT                 AG^CT
AspHI         171  GAGCAC               G[AT]GC[AT]^C
BbeI           58  GGCGCC               GGCGC^C
BbsI          325  GAAGAC               GAAGAC(2/6)
BcgI          422  GCAATAGAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          176  CCGGG                CC^[GC]GG
BpuAI         325  GAAGAC               GAAGAC(2/6)
BsaAI         628  TACGTG               [CT]AC^GT[GA]
BsaBI         276  GATAGCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI          58  GGCGCC               G[GA]^CG[CT]C
BsiHKAI       171  GAGCAC               G[AT]GC[AT]^C
BsmFI         557  GTCCC                GGGAC(10/14)
BsrDI         227  CATTGC               GCAATG(2/0)
BsrFI         106  GCCGGC               [GA]^CCGG[CT]
BstAPI        235  GCAACCCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         182  AGATCC               [GA]^GATC[CT]
Cfr10I        106  GCCGGC               [GA]^CCGG[CT]
Cfr13I        590  GGCCC                G^G[ACGT]CC
DdeI          217  CTGAG                C^T[ACGT]AG
DraII         589  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EarI          493  CTCTTC               CTCTTC(1/4)
EcoO109I      589  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRI         439  GAATTC               G^AATTC
EcoT14I       586  CCAAGG               C^C[AT][AT]GG
HaeII          58  GGCGCC               [GA]GCGC^[CT]
Hin1I          58  GGCGCC               G[GA]^CG[CT]C
KasI           58  GGCGCC               G^GCGCC
Ksp632I       493  CTCTTC               CTCTTC(1/4)
MaeII         629  ACGT                 A^CGT
MaeIII        121  GTGAC                ^GT[ACGT]AC
MamI          276  GATAGCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          182  AGATCC               [GA]^GATC[CT]
NaeI          106  GCCGGC               GCC^GGC
NarI           58  GGCGCC               GG^CGCC
NciI          176  CCGGG                CC^[GC]GG
NgoMIV        106  GCCGGC               G^CCGGC
PflMI         407  CCACAGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          202  GACTC                GAGTC(4/5)
PshAI         456  GACCCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI           16  CTGCAG               CTGCA^G
PvuII          19  CAGCTG               CAG^CTG
Sau96I        590  GGCCC                G^G[ACGT]CC
SfaNI         260  GCATC                GCATC(5/9)
SfcI           16  CTGCAG               C^T[GA][CT]AG
SfoI           58  GGCGCC               GGC^GCC
StyI          586  CCAAGG               C^C[AT][AT]GG
Tsp45I        121  GTGAC                ^GT[GC]AC
Van91I        407  CCACAGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         182  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccII           9  CGCG                 CG^CG
              369  CGCG                
AcsI          439  GAATTC               [GA]^AATT[CT]
              478  GAATTT              
AfaI          138  GTAC                 GT^AC
              474  GTAC                
AlwI          183  GATCC                GGATC(4/5)
               53  GGATC               
ApoI          439  GAATTC               [GA]^AATT[CT]
              478  GAATTT              
BalI            2  TGGCCA               TGG^CCA
              290  TGGCCA              
BanI           58  GGCGCC               G^G[CT][GA]CC
              535  GGTGCC              
BmyI          171  GAGCAC               G[AGT]GC[ACT]^C
              536  GTGCCC              
BsgI          605  CTGCAC               GTGCAG(16/14)
              337  GTGCAG              
Bsp1286I      171  GAGCAC               G[AGT]GC[ACT]^C
              536  GTGCCC              
BspMI         603  ACCTGC               ACCTGC(4/8)
              385  GCAGGT              
BstUI           9  CGCG                 CG^CG
              369  CGCG                
Cac8I         106  GCCGGC               GC[ACGT]^[ACGT]GC
              231  GCAAGC              
CfoI           59  GCGC                 GCG^C
              368  GCGC                
DpnII          54  GATC                 ^GATC
              183  GATC                
EaeI            2  TGGCCA               [CT]^GGCC[GA]
              290  TGGCCA              
FauI           10  GCGGG                CCCGC(4/6)
              617  GCGGG               
HapII         107  CCGG                 C^CGG
              176  CCGG                
HhaI           59  GCGC                 GCG^C
              368  GCGC                
HinP1I         59  GCGC                 G^CGC
              368  GCGC                
HpaII         107  CCGG                 C^CGG
              176  CCGG                
MboI           54  GATC                 ^GATC
              183  GATC                
MluNI           2  TGGCCA               TGG^CCA
              290  TGGCCA              
MscI            2  TGGCCA               TGG^CCA
              290  TGGCCA              
MspA1I         19  CAGCTG               C[AC]G^C[GT]G
              615  CAGCGG              
MspI          107  CCGG                 C^CGG
              176  CCGG                
MvnI            9  CGCG                 CG^CG
              369  CGCG                
MwoI          235  GCAACCCGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              385  GCAGGTCTGGC         
NdeII          54  GATC                 ^GATC
              183  GATC                
NlaIV          58  GGCGCC               GG[ACGT]^[ACGT]CC
              535  GGTGCC              
RsaI          138  GTAC                 GT^AC
              474  GTAC                
Sau3AI         54  GATC                 ^GATC
              183  GATC                
TfiI          213  GAATC                G^A[AT]TC
              428  GAATC               
Tsp509I       440  AATT                 ^AATT
              479  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AciI        BbvI        BsaJI       BsiYI       BslI        
BsrI        BssKI       BstF5I      BstNI       EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       ItaI        
MboII       MnlI        MvaI        NlaIII      ScrFI       
TseI        TspRI       
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