(Linear) (Six-base) MAPSORT of: KIBB3085.seq  Check: 1936  from: 1  to: 633



KIBB3085.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      94     244     633 
   Size:         94     150     389

  Fragments arranged by size: 

                 389     150      94
 
ApoI r'AATT_y

Cuts at:      0     439     478     633 
   Size:        439      39     155

  Fragments arranged by size: 

                 439     155      39
 
BanI G'GyrC_C

Cuts at:      0      58     535     633 
   Size:         58     477      98

  Fragments arranged by size: 

                 477      98      58
 
BbeI G_GCGC'C

Cuts at:      0      62     633 
   Size:         62     571
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     332     633 
   Size:        332     301
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     411     445     633 
   Size:        411      34     188

  Fragments arranged by size: 

                 411     188      34
 
Bme1580I G_kGCm'C

Cuts at:      0     540     633 
   Size:        540      93
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      78     454     633 
   Size:         78     376     179

  Fragments arranged by size: 

                 376     179      78
 
BsaAI yAC'GTr

Cuts at:      0     630     633 
   Size:        630       3
 
BsaBI GATnn'nnATC

Cuts at:      0     280     633 
   Size:        280     353
 
BsaHI Gr'CG_yC

Cuts at:      0      59     633 
   Size:         59     574
 
BseYI C'CCAG_C

Cuts at:      0     503     633 
   Size:        503     130
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     358     590     633 
   Size:        358     232      43

  Fragments arranged by size: 

                 358     232      43
 
BsiHKAI G_wGCw'C

Cuts at:      0     175     633 
   Size:        175     458
 
Bsp1286I G_dGCh'C

Cuts at:      0     175     540     633 
   Size:        175     365      93

  Fragments arranged by size: 

                 365     175      93
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     376     612     633 
   Size:        376     236      21

  Fragments arranged by size: 

                 376     236      21
 
BsrDI GCAATG_nn'

Cuts at:      0     226     633 
   Size:        226     407
 
BsrFI r'CCGG_y

Cuts at:      0     106     633 
   Size:        106     527
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     241     633 
   Size:        241     392
 
BstYI r'GATC_y

Cuts at:      0     182     633 
   Size:        182     451
 
BtsI GCAGTG_nn'

Cuts at:      0      71     633 
   Size:         71     562
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     269     304     633 
   Size:        269      35     329

  Fragments arranged by size: 

                 329     269      35
 
EaeI y'GGCC_r

Cuts at:      0       2     290     633 
   Size:          2     288     343

  Fragments arranged by size: 

                 343     288       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     499     633 
   Size:        499     134
 
EcoO109I rG'GnC_Cy

Cuts at:      0     590     633 
   Size:        590      43
 
EcoRI G'AATT_C

Cuts at:      0     439     633 
   Size:        439     194
 
HaeII r_GCGC'y

Cuts at:      0      62     633 
   Size:         62     571
 
KasI G'GCGC_C

Cuts at:      0      58     633 
   Size:         58     575
 
MscI TGG'CCA

Cuts at:      0       4     292     633 
   Size:          4     288     341

  Fragments arranged by size: 

                 341     288       4
 
MspA1I CmG'CkG

Cuts at:      0      21     617     633 
   Size:         21     596      16

  Fragments arranged by size: 

                 596      21      16
 
NaeI GCC'GGC

Cuts at:      0     108     633 
   Size:        108     525
 
NarI GG'CG_CC

Cuts at:      0      59     633 
   Size:         59     574
 
NgoMIV G'CCGG_C

Cuts at:      0     106     633 
   Size:        106     527
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     413     633 
   Size:        413     220
 
PshAI GACnn'nnGTC

Cuts at:      0     460     633 
   Size:        460     173
 
PstI C_TGCA'G

Cuts at:      0      20     633 
   Size:         20     613
 
PvuII CAG'CTG

Cuts at:      0      21     633 
   Size:         21     612
 
SfcI C'TryA_G

Cuts at:      0      16     633 
   Size:         16     617
 
SfoI GGC'GCC

Cuts at:      0      60     633 
   Size:         60     573
 
StyI C'CwwG_G

Cuts at:      0     586     633 
   Size:        586      47
 

 Enzymes that do cut:

     AlfI     ApoI     BanI     BbeI     BbsI     BcgI Bme1580I     BmrI
    BsaAI    BsaBI    BsaHI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI
    BsrDI    BsrFI   BstAPI    BstYI     BtsI    CspCI     EaeI     EarI
 EcoO109I    EcoRI    HaeII     KasI     MscI   MspA1I     NaeI     NarI
   NgoMIV    PflMI    PshAI     PstI    PvuII     SfcI     SfoI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaWI    BsaXI
    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI  Eco57MI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MslI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI