Restriction map for pF1KB4753
##################################
# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AgeI        
AhdI        AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrFI       
BsrGI       BsrI        BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HaeII       HaeIII      HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        KasI        
KpnI        Ksp632I     KspI        MaeI        MamI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        SexAI       SfaNI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       Tsp45I      Tsp509I     Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AfaI          126  GTAC                 GT^AC
AflIII         77  ACACGT               A^C[GA][CT]GT
Alw44I         91  GTGCAC               G^TGCAC
ApaLI          91  GTGCAC               G^TGCAC
AspHI          91  GTGCAC               G[AT]GC[AT]^C
BbrPI          78  CACGTG               CAC^GTG
BcnI           16  CCCGG                CC^[GC]GG
BpmI          315  CTGGAG               CTGGAG(16/14)
BseRI          43  GAGGAG               GAGGAG(10/8)
BsiHKAI        91  GTGCAC               G[AT]GC[AT]^C
BsrBI         252  CCGCTC               CCGCTC(-3/-3)
BsrDI         293  GCAATG               GCAATG(2/0)
BssSI         224  CTCGTG               CACGAG(-5/-1)
BstAPI         74  GCAACACGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         173  CCTGG                CC^[AT]GG
Cac8I          70  GCCTGC               GC[ACGT]^[ACGT]GC
DdeI          299  CTCAG                C^T[ACGT]AG
DpnII          36  GATC                 ^GATC
EcoRII        173  CCTGG                ^CC[AT]GG
HapII          17  CCGG                 C^CGG
HincII        184  GTTGAC               GT[CT]^[GA]AC
HindII        184  GTTGAC               GT[CT]^[GA]AC
HpaII          17  CCGG                 C^CGG
HphI          101  TCACC                GGTGA(8/7)
MaeIII        112  GTAAC                ^GT[ACGT]AC
MboI           36  GATC                 ^GATC
MslI          220  CATCCTCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           17  CCGG                 C^CGG
MvaI          173  CCTGG                CC^[AT]GG
NciI           16  CCCGG                CC^[GC]GG
NdeII          36  GATC                 ^GATC
NlaIII        237  CATG                 CATG^
PmaCI          78  CACGTG               CAC^GTG
PmlI           78  CACGTG               CAC^GTG
PvuII         167  CAGCTG               CAG^CTG
RsaI          126  GTAC                 GT^AC
Sau3AI         36  GATC                 ^GATC
TspRI         301  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AluI          168  AGCT                 AG^CT
              242  AGCT                
BmyI           81  GTGCCC               G[AGT]GC[ACT]^C
               91  GTGCAC              
BsaAI          78  CACGTG               [CT]AC^GT[GA]
               88  TACGTG              
BsaJI          15  CCCCGG               C^C[ACGT][ACGT]GG
              173  CCTGGG              
BsmFI          48  GGGAC                GGGAC(10/14)
              206  GTCCC               
Bsp1286I       81  GTGCCC               G[AGT]GC[ACT]^C
               91  GTGCAC              
BssKI          16  CCCGG                ^CC[ACGT]GG
              173  CCTGG               
BstF5I        220  CATCC                GGATG(2/0)
              147  GGATG               
FokI          220  CATCC                GGATG(9/13)
              147  GGATG               
HinfI         187  GACTC                G^A[ACGT]TC
              199  GAGTC               
MaeII          79  ACGT                 A^CGT
               89  ACGT                
MspA1I        167  CAGCTG               C[AC]G^C[GT]G
              312  CCGCTG              
PleI          187  GACTC                GAGTC(4/5)
              199  GAGTC               
PstI          194  CTGCAG               CTGCA^G
              257  CTGCAG              
ScrFI          16  CCCGG                CC^[ACGT]GG
              173  CCTGG               
SfcI          194  CTGCAG               C^T[GA][CT]AG
              257  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AciI        BbvI        BsiYI       BslI        FauI        
Fnu4HI      ItaI        MnlI        MwoI        TseI        
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