Restriction map for pF1KB6683
 (Circular) (Six-base) MAPSORT of: pF1KB6683.seq  Check: 6708  from: 1  to: 4394



pF1KB6683  4394 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1168    1168 
   Size:       4394
 
AccI GT'mk_AC

Cuts at:   1432    1432 
   Size:       4394
 
Acc65I G'GTAC_C

Cuts at:   1410    1410 
   Size:       4394
 
AclI AA'CG_TT

Cuts at:   4234    4234 
   Size:       4394
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1393    2219    2651    3398    3666    1393 
   Size:        826     432     747     268    2121

  Fragments arranged by size: 

                2121     826     747     432     268
 
AflIII A'CryG_T

Cuts at:   1746    2850    3827    1746 
   Size:       1104     977    2313

  Fragments arranged by size: 

                2313    1104     977
 
AgeI A'CCGG_T

Cuts at:   2782    2782 
   Size:       4394
 
AlwNI CAG_nnn'CTG

Cuts at:   1699    3266    1699 
   Size:       1567    2827
 
ApaLI G'TGCA_C

Cuts at:   3164    3164 
   Size:       4394
 
ApoI r'AATT_y

Cuts at:    216     614     861     964    1398    3747    4003     216 
   Size:        398     247     103     434    2349     256     607

  Fragments arranged by size: 

                2349     607     434     398     256     247     103
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4394
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4394
 
AvaI C'yCGr_G

Cuts at:    271    1414     271 
   Size:       1143    3251
 
BamHI G'GATC_C

Cuts at:   1419    1419 
   Size:       4394
 
BanI G'GyrC_C

Cuts at:    181    1410    1824    2072    2107     181 
   Size:       1229     414     248      35    2468

  Fragments arranged by size: 

                2468    1229     414     248      35
 
BanII G_rGCy'C

Cuts at:   1408    2438    1408 
   Size:       1030    3364
 
BbeI G_GCGC'C

Cuts at:    185    1828    2076     185 
   Size:       1643     248    2503

  Fragments arranged by size: 

                2503    1643     248
 
BciVI GTATCCnnnnn_n'

Cuts at:   2292    3053    4394    2292 
   Size:        761    1341    2292

  Fragments arranged by size: 

                2292    1341     761
 
BclI T'GATC_A

Cuts at:   1914    1914 
   Size:       4394
 
BfrBI ATG'CAT

Cuts at:    458     509     458 
   Size:         51    4343
 
BglI GCCn_nnn'nGGC

Cuts at:    123     123 
   Size:       4394
 
BglII A'GATC_T

Cuts at:   1909    3946    1909 
   Size:       2037    2357
 
BlpI GC'TnA_GC

Cuts at:   1506    1506 
   Size:       4394
 
Bme1580I G_kGCm'C

Cuts at:   2019    2112    3168    2019 
   Size:         93    1056    3245

  Fragments arranged by size: 

                3245    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    402    1606    1687    2022    4166     402 
   Size:       1204      81     335    2144     630

  Fragments arranged by size: 

                2144    1204     630     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2761    2761 
   Size:       4394
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1107    1107 
   Size:       4394
 
Bpu10I CC'TnA_GC

Cuts at:    523     523 
   Size:       4394
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1201    1570    2449    2941    3239    1201 
   Size:        369     879     492     298    2356

  Fragments arranged by size: 

                2356     879     492     369     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   1271    4098    1271 
   Size:       2827    1567
 
BsaAI yAC'GTr

Cuts at:   1747    1747 
   Size:       4394
 
BsaBI GATnn'nnATC

Cuts at:      6    1908    3945       6 
   Size:       1902    2037     455

  Fragments arranged by size: 

                2037    1902     455
 
BsaHI Gr'CG_yC

Cuts at:    182    1825    2073    2775    4271     182 
   Size:       1643     248     702    1496     305

  Fragments arranged by size: 

                1643    1496     702     305     248
 
BsaWI w'CCGG_w

Cuts at:    345    1583    2104    2782    3056    3203    3614     345 
   Size:       1238     521     678     274     147     411    1125

  Fragments arranged by size: 

                1238    1125     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1819    2577    3154    3650    1819 
   Size:        758     577     496    2563

  Fragments arranged by size: 

                2563     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    228    1282     228 
   Size:       1054    3340
 
BsiEI CG_ry'CG

Cuts at:    104    1936    1982    3190     104 
   Size:       1832      46    1208    1308

  Fragments arranged by size: 

                1832    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1408    2186    2376    3168    1408 
   Size:        778     190     792    2634

  Fragments arranged by size: 

                2634     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:   1408    2019    2112    2186    2376    2438    3168    1408 
   Size:        611      93      74     190      62     730    2634

  Fragments arranged by size: 

                2634     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:    345    3614     345 
   Size:       3269    1125
 
BspHI T'CATG_A

Cuts at:   3570    3570 
   Size:       4394
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1168    1444    1658    1960    2341    1168 
   Size:        276     214     302     381    3221

  Fragments arranged by size: 

                3221     381     302     276     214
 
BsrBI CCG'CTC

Cuts at:     50    2686    2740    4224    4389      50 
   Size:       2636      54    1484     165      55

  Fragments arranged by size: 

                2636    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    850    2306     850 
   Size:       1456    2938
 
BsrFI r'CCGG_y

Cuts at:    117     157    2392    2573    2782     117 
   Size:         40    2235     181     209    1729

  Fragments arranged by size: 

                2235    1729     209     181      40
 
BssSI C'ACGA_G

Cuts at:   2665    3023    2665 
   Size:        358    4036
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1771    1771 
   Size:       4394
 
BstBI TT'CG_AA

Cuts at:   2755    2755 
   Size:       4394
 
BstXI CCAn_nnnn'nTGG

Cuts at:    655     655 
   Size:       4394
 
BstYI r'GATC_y

Cuts at:      1    1419    1886    1909    2244    2490    3502    3588    3600 
   Size:       1418     467      23     335     246    1012      86      12

Cuts at:   3600    3946       1 
   Size:        346     449

  Fragments arranged by size: 

                1418    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4394
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2344    2525    2688    2344 
   Size:        181     163    4050

  Fragments arranged by size: 

                4050     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1637    1637 
   Size:       4394
 
DraI TTT'AAA

Cuts at:   1263    1393    1263 
   Size:        130    4264
 
DrdI GACnn_nn'nnGTC

Cuts at:   2100    2958    2100 
   Size:        858    3536
 
EaeI y'GGCC_r

Cuts at:    115    1979    2153    2544    2571    3916     115 
   Size:       1864     174     391      27    1345     593

  Fragments arranged by size: 

                1864    1345     593     391     174      27
 
EagI C'GGCC_G

Cuts at:   1979    1979 
   Size:       4394
 
EarI CTCTTCn'nnn_

Cuts at:   2417    2627    2417 
   Size:        210    4184
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2602    2912    3058    4204    2602 
   Size:        310     146    1146    2792

  Fragments arranged by size: 

                2792    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1406    1406 
   Size:       4394
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1107    1393    2219    2651    3398    3666    1107 
   Size:        286     826     432     747     268    1835

  Fragments arranged by size: 

                1835     826     747     432     286     268
 
EcoO109I rG'GnC_Cy

Cuts at:    404    1533     404 
   Size:       1129    3265
 
EcoRI G'AATT_C

Cuts at:   1398    1398 
   Size:       4394
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2413    2413 
   Size:       4394
 
FspI TGC'GCA

Cuts at:   2175    2175 
   Size:       4394
 
HaeII r_GCGC'y

Cuts at:    145     185    1828    2076    3098    4077     145 
   Size:         40    1643     248    1022     979     462

  Fragments arranged by size: 

                1643    1022     979     462     248      40
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    249     281     375     407     469     501     973    1005    2236 
   Size:         32      94      32      62      32     472      32    1231

Cuts at:   2236    2266    2268    2298     249 
   Size:         30       2      30    2345

  Fragments arranged by size: 

                2345    1231     472      94      62      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:   1433    1433 
   Size:       4394
 
KasI G'GCGC_C

Cuts at:    181    1824    2072     181 
   Size:       1643     248    2503

  Fragments arranged by size: 

                2503    1643     248
 
KpnI G_GTAC'C

Cuts at:   1414    1414 
   Size:       4394
 
MluI A'CGCG_T

Cuts at:   3827    3827 
   Size:       4394
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3065    3249    3065 
   Size:        184    4210
 
MscI TGG'CCA

Cuts at:   2155    2155 
   Size:       4394
 
MslI CAynn'nnrTG

Cuts at:    623     827    1147    2383    2510     623 
   Size:        204     320    1236     127    2507

  Fragments arranged by size: 

                2507    1236     320     204     127
 
MspA1I CmG'CkG

Cuts at:   1505    1819    2179    3192    3437    1505 
   Size:        314     360    1013     245    2462

  Fragments arranged by size: 

                2462    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:    119     159    2575     119 
   Size:         40    2416    1938

  Fragments arranged by size: 

                2416    1938      40
 
NarI GG'CG_CC

Cuts at:    182    1825    2073     182 
   Size:       1643     248    2503

  Fragments arranged by size: 

                2503    1643     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4394
 
NgoMIV G'CCGG_C

Cuts at:    117     157    2573     117 
   Size:         40    2416    1938

  Fragments arranged by size: 

                2416    1938      40
 
NsiI A_TGCA'T

Cuts at:    460     511     460 
   Size:         51    4343
 
NspI r_CATG'y

Cuts at:   1447    1947    2854    1447 
   Size:        500     907    2987

  Fragments arranged by size: 

                2987     907     500
 
PciI A'CATG_T

Cuts at:   2850    2850 
   Size:       4394
 
PflMI CCAn_nnn'nTGG

Cuts at:    113     720     113 
   Size:        607    3787
 
PmeI GTTT'AAAC

Cuts at:   1393    1393 
   Size:       4394
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3533    3565    3533 
   Size:         32    4362
 
PpuMI rG'GwC_Cy

Cuts at:    404     404 
   Size:       4394
 
PstI C_TGCA'G

Cuts at:   1441    2126    1441 
   Size:        685    3709
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4394
 
PvuII CAG'CTG

Cuts at:   1819    2179    1819 
   Size:        360    4034
 
RsrII CG'GwC_CG

Cuts at:   2589    2589 
   Size:       4394
 
SacI G_AGCT'C

Cuts at:   1408    1408 
   Size:       4394
 
SalI G'TCGA_C

Cuts at:   1431    1431 
   Size:       4394
 
SanDI GG'GwC_CC

Cuts at:    404     404 
   Size:       4394
 
SapI GCTCTTCn'nnn_

Cuts at:   2417    2627    2417 
   Size:        210    4184
 
SbfI CC_TGCA'GG

Cuts at:   1441    1441 
   Size:       4394
 
ScaI AGT'ACT

Cuts at:   1193    3911    1193 
   Size:       2718    1676
 
SfcI C'TryA_G

Cuts at:     33    1352    1437    2122    3115    3306      33 
   Size:       1319      85     685     993     191    1121

  Fragments arranged by size: 

                1319    1121     993     685     191      85
 
SfoI GGC'GCC

Cuts at:    183    1826    2074     183 
   Size:       1643     248    2503

  Fragments arranged by size: 

                2503    1643     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4394
 
SgrAI Cr'CCGG_yG

Cuts at:    157     157 
   Size:       4394
 
SmaI CCC'GGG

Cuts at:   1416    1416 
   Size:       4394
 
SmlI C'TyrA_G

Cuts at:   1216    1549    2464    2956    3218    1216 
   Size:        333     915     492     262    2392

  Fragments arranged by size: 

                2392     915     492     333     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4394
 
SphI G_CATG'C

Cuts at:   1447    1947    1447 
   Size:        500    3894
 
StyI C'CwwG_G

Cuts at:    107    1334    1528    1882     107 
   Size:       1227     194     354    2619

  Fragments arranged by size: 

                2619    1227     354     194
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1977    2110    2569    2778    1977 
   Size:        133     459     209    3593

  Fragments arranged by size: 

                3593     459     209     133
 
TatI w'GTAC_w

Cuts at:   1191    3909    1191 
   Size:       2718    1676
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3911    3943    3911 
   Size:         32    4362
 
Tth111I GACn'n_nGTC

Cuts at:   2191    2191 
   Size:       4394
 
XbaI T'CTAG_A

Cuts at:   1425    1425 
   Size:       4394
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1889    1889 
   Size:       4394
 
XmaI C'CCGG_G

Cuts at:   1414    1414 
   Size:       4394
 
XmnI GAAnn'nnTTC

Cuts at:    614     614 
   Size:       4394
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI
     NspI     PciI    PflMI     PmeI     PpiI    PpuMI     PstI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BmgBI     BmtI
    BsaXI    BseRI    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII
  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     PacI     PasI     PfoI     PmlI    PshAI     PsiI   PspOMI    PspXI
     PsrI    SacII    SexAI     SfiI    SnaBI     SrfI     SspI     StuI
     SwaI     XhoI     ZraI
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