Restriction map for pF1KB6694
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AcyI        
AflII       AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AspI        AvaII       AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BpuAI       BsaBI       
BsaHI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraII       DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoRI       EcoT22I     FauI        FbaI        
FseI        FspI        HgaI        Hin1I       KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MflI        MluI        MroI        MslI        MunI        
MvnI        NarI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SbfI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tth111I     XbaI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccI          757  GTCTAC               GT^[AC][GT]AC
AflIII         42  ACACGT               A^C[GA][CT]GT
Asp700I       901  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          398  CCCGAG               C^[CT]CG[GA]G
BalI           72  TGGCCA               TGG^CCA
BanII         153  GGGCTC               G[GA]GC[CT]^C
BcnI          820  CCCGG                CC^[GC]GG
BglI          409  GCCCTTCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          153  GGGCTC               G[AGT]GC[ACT]^C
BsaAI          43  CACGTA               [CT]AC^GT[GA]
BsaI          241  GAGACC               GGTCTC(1/5)
BsoBI         398  CCCGAG               C^[CT]CG[GA]G
Bsp1286I      153  GGGCTC               G[AGT]GC[ACT]^C
BsrFI        1063  GCCGGC               [GA]^CCGG[CT]
Cfr10I       1063  GCCGGC               [GA]^CCGG[CT]
Cfr13I       1168  GGCCC                G^G[ACGT]CC
DraIII        425  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          328  GACTGTGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV         220  GATATC               GAT^ATC
EcoT14I       625  CCTTGG               C^C[AT][AT]GG
HaeII        1060  AGCGCC               [GA]GCGC^[CT]
HindIII       890  AAGCTT               A^AGCTT
HpaI          454  GTTAAC               GTT^AAC
MluNI          72  TGGCCA               TGG^CCA
MscI           72  TGGCCA               TGG^CCA
MspA1I       1010  CAGCTG               C[AC]G^C[GT]G
NaeI         1063  GCCGGC               GCC^GGC
NciI          820  CCCGG                CC^[GC]GG
NgoMIV       1063  GCCGGC               G^CCGGC
PflMI         574  CCAATTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI           15  GACTC                GAGTC(4/5)
PvuII        1010  CAGCTG               CAG^CTG
SapI          904  GCTCTTC              GCTCTTC(1/4)
Sau96I       1168  GGCCC                G^G[ACGT]CC
ScaI          251  AGTACT               AGT^ACT
SfcI          483  CTACAG               C^T[GA][CT]AG
SmlI          685  CTTGAG               C^T[CT][GA]AG
StyI          625  CCTTGG               C^C[AT][AT]GG
Van91I        574  CCAATTTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          901  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AciI           92  GCGG                 CCGC(-3/-1)
             1300  GCGG                
AclI          850  AACGTT               AA^CGTT
              970  AACGTT              
AfaI          252  GTAC                 GT^AC
              779  GTAC                
BlpI          193  GCTGAGC              GC^T[ACGT]AGC
             1117  GCTGAGC             
Bpu1102I      193  GCTGAGC              GC^T[ACGT]AGC
             1117  GCTGAGC             
BsmFI         313  GGGAC                GGGAC(10/14)
              914  GGGAC               
BsrDI        1101  CATTGC               GCAATG(2/0)
              662  GCAATG              
BstEII        831  GGTCACC              G^GT[ACGT]ACC
             1350  GGTCACC             
BstPI         831  GGTCACC              G^GT[ACGT]ACC
             1350  GGTCACC             
CelII         193  GCTGAGC              GC^T[ACGT]AGC
             1117  GCTGAGC             
CfoI          738  GCGC                 GCG^C
             1061  GCGC                
DpnII          81  GATC                 ^GATC
             1138  GATC                
EaeI           72  TGGCCA               [CT]^GGCC[GA]
              792  TGGCCG              
EarI          905  CTCTTC               CTCTTC(1/4)
              492  GAAGAG              
EcoO65I       831  GGTCACC              G^GT[ACGT]ACC
             1350  GGTCACC             
HapII         821  CCGG                 C^CGG
             1064  CCGG                
HhaI          738  GCGC                 GCG^C
             1061  GCGC                
HinP1I        738  GCGC                 G^CGC
             1061  GCGC                
HincII        454  GTTAAC               GT[CT]^[GA]AC
              847  GTCAAC              
HindII        454  GTTAAC               GT[CT]^[GA]AC
              847  GTCAAC              
HpaII         821  CCGG                 C^CGG
             1064  CCGG                
Ksp632I       905  CTCTTC               CTCTTC(1/4)
              492  GAAGAG              
MboI           81  GATC                 ^GATC
             1138  GATC                
MspI          821  CCGG                 C^CGG
             1064  CCGG                
NdeII          81  GATC                 ^GATC
             1138  GATC                
Psp1406I      850  AACGTT               AA^CGTT
              970  AACGTT              
RsaI          252  GTAC                 GT^AC
              779  GTAC                
Sau3AI         81  GATC                 ^GATC
             1138  GATC                
SphI          405  GCATGC               GCATG^C
             1105  GCATGC              
TaqI          101  TCGA                 T^CGA
              875  TCGA                
TfiI           49  GATTC                G^A[AT]TC
              466  GATTC               
TthHB8I       101  TCGA                 T^CGA
              875  TCGA                
XcmI          574  CCAATTTCTGGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1199  CCATTGTTGATATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AcsI        AluI        ApoI        BbvI        BpmI        
BsaJI       BsiYI       BslI        BsmAI       BsrI        
BssKI       BstF5I      BstNI       Cac8I       DdeI        
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        MaeII       MaeIII      MboII       
MnlI        MseI        MvaI        MwoI        NlaIII      
NlaIV       NspI        ScrFI       SfaNI       Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       
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