Restriction map for pF1KB6844
 (Circular) (Six-base) MAPSORT of: pF1KB6844.seq  Check: 6183  from: 1  to: 3689



pF1KB6844  3689 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AatII G_ACGT'C

Cuts at:    338     338 
   Size:       3689
 
AccI GT'mk_AC

Cuts at:    727     727 
   Size:       3689
 
Acc65I G'GTAC_C

Cuts at:    398     705     398 
   Size:        307    3382
 
AclI AA'CG_TT

Cuts at:   3529    3529 
   Size:       3689
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    622    1514    1946    2693    2961     622 
   Size:        892     432     747     268    1350

  Fragments arranged by size: 

                1350     892     747     432     268
 
AflIII A'CryG_T

Cuts at:   1041    2145    3122    1041 
   Size:       1104     977    1608

  Fragments arranged by size: 

                1608    1104     977
 
AgeI A'CCGG_T

Cuts at:   2077    2077 
   Size:       3689
 
AlwNI CAG_nnn'CTG

Cuts at:    534     994    2561     534 
   Size:        460    1567    1662

  Fragments arranged by size: 

                1662    1567     460
 
ApaLI G'TGCA_C

Cuts at:    672    2459     672 
   Size:       1787    1902
 
ApoI r'AATT_y

Cuts at:    693    3042    3298     693 
   Size:       2349     256    1084

  Fragments arranged by size: 

                2349    1084     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3689
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3689
 
AvaI C'yCGr_G

Cuts at:    545     709     545 
   Size:        164    3525
 
BamHI G'GATC_C

Cuts at:    714     714 
   Size:       3689
 
BanI G'GyrC_C

Cuts at:    142     398     413     448     510     705    1119    1367    1402 
   Size:        256      15      35      62     195     414     248      35

Cuts at:   1402     142 
   Size:       2429

  Fragments arranged by size: 

                2429     414     256     248     195      62      35      35
                  15
 
BanII G_rGCy'C

Cuts at:    367     703    1733     367 
   Size:        336    1030    2323

  Fragments arranged by size: 

                2323    1030     336
 
BbeI G_GCGC'C

Cuts at:    146    1123    1371     146 
   Size:        977     248    2464

  Fragments arranged by size: 

                2464     977     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    160     610     672     160 
   Size:        450      62    3177

  Fragments arranged by size: 

                3177     450      62
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124     663     697      90 
   Size:         34     539      34    3082

  Fragments arranged by size: 

                3082     539      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   1587    2348    3689    1587 
   Size:        761    1341    1587

  Fragments arranged by size: 

                1587    1341     761
 
BclI T'GATC_A

Cuts at:    167    1209     167 
   Size:       1042    2647
 
BglI GCCn_nnn'nGGC

Cuts at:    112     112 
   Size:       3689
 
BglII A'GATC_T

Cuts at:   1204    3241    1204 
   Size:       2037    1652
 
BlpI GC'TnA_GC

Cuts at:    579     801     579 
   Size:        222    3467
 
Bme1580I G_kGCm'C

Cuts at:    451     676    1314    1407    2463     451 
   Size:        225     638      93    1056    1677

  Fragments arranged by size: 

                1677    1056     638     225      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    643     901     982    1317    3461     643 
   Size:        258      81     335    2144     871

  Fragments arranged by size: 

                2144     871     335     258      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    573    2056     573 
   Size:       1483    2206
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    592     592 
   Size:       3689
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    865    1744    2236    2534     865 
   Size:        879     492     298    2020

  Fragments arranged by size: 

                2020     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3393    3393 
   Size:       3689
 
BsaAI yAC'GTr

Cuts at:   1042    1042 
   Size:       3689
 
BsaBI GATnn'nnATC

Cuts at:      6    1203    3240       6 
   Size:       1197    2037     455

  Fragments arranged by size: 

                2037    1197     455
 
BsaHI Gr'CG_yC

Cuts at:    143     335    1120    1368    2070    3566     143 
   Size:        192     785     248     702    1496     266

  Fragments arranged by size: 

                1496     785     702     266     248     192
 
BsaWI w'CCGG_w

Cuts at:    878    1399    2077    2351    2498    2909     878 
   Size:        521     678     274     147     411    1658

  Fragments arranged by size: 

                1658     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    590    1114    1872    2449    2945     590 
   Size:        524     758     577     496    1334

  Fragments arranged by size: 

                1334     758     577     524     496
 
BsiEI CG_ry'CG

Cuts at:    104     303    1231    1277    2485     104 
   Size:        199     928      46    1208    1308

  Fragments arranged by size: 

                1308    1208     928     199      46
 
BsiHKAI G_wGCw'C

Cuts at:    580     638     676     703    1481    1671    2463     580 
   Size:         58      38      27     778     190     792    1806

  Fragments arranged by size: 

                1806     792     778     190      58      38      27
 
Bsp1286I G_dGCh'C

Cuts at:    367     451     580     638     676     703    1314    1407    1481 
   Size:         84     129      58      38      27     611      93      74

Cuts at:   1481    1671    1733    2463     367 
   Size:        190      62     730    1593

  Fragments arranged by size: 

                1593     730     611     190     129      93      84      74
                  62      58      38      27
 
BspEI T'CCGG_A

Cuts at:   2909    2909 
   Size:       3689
 
BspHI T'CATG_A

Cuts at:   2865    2865 
   Size:       3689
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    739     953    1255    1636     739 
   Size:        214     302     381    2792

  Fragments arranged by size: 

                2792     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     489    1981    2035    3519    3684      50 
   Size:        439    1492      54    1484     165      55

  Fragments arranged by size: 

                1492    1484     439     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1601    1601 
   Size:       3689
 
BsrFI r'CCGG_y

Cuts at:   1687    1868    2077    1687 
   Size:        181     209    3299

  Fragments arranged by size: 

                3299     209     181
 
BssSI C'ACGA_G

Cuts at:   1960    2318    1960 
   Size:        358    3331
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1066    1066 
   Size:       3689
 
BstBI TT'CG_AA

Cuts at:   2050    2050 
   Size:       3689
 
BstXI CCAn_nnnn'nTGG

Cuts at:    240     441     240 
   Size:        201    3488
 
BstYI r'GATC_y

Cuts at:      1     714    1181    1204    1539    1785    2797    2883    2895 
   Size:        713     467      23     335     246    1012      86      12

Cuts at:   2895    3241       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     713     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    146     539     146 
   Size:        393    3296
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1639    1820    1983    1639 
   Size:        181     163    3345

  Fragments arranged by size: 

                3345     181     163
 
BtsI GCAGTG_nn'

Cuts at:    417     932     417 
   Size:        515    3174
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    198     233     198 
   Size:         35    3654
 
DraI TTT'AAA

Cuts at:    688     688 
   Size:       3689
 
DrdI GACnn_nn'nnGTC

Cuts at:   1395    2253    1395 
   Size:        858    2831
 
EaeI y'GGCC_r

Cuts at:    310     536    1274    1448    1839    1866    3211     310 
   Size:        226     738     174     391      27    1345     788

  Fragments arranged by size: 

                1345     788     738     391     226     174      27
 
EagI C'GGCC_G

Cuts at:   1274    1274 
   Size:       3689
 
EarI CTCTTCn'nnn_

Cuts at:   1712    1922    1712 
   Size:        210    3479
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1897    2207    2353    3499    1897 
   Size:        310     146    1146    2087

  Fragments arranged by size: 

                2087    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    701     701 
   Size:       3689
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    592     622    1514    1946    2693    2961     592 
   Size:         30     892     432     747     268    1320

  Fragments arranged by size: 

                1320     892     747     432     268      30
 
EcoO109I rG'GnC_Cy

Cuts at:    531     597     828     531 
   Size:         66     231    3392

  Fragments arranged by size: 

                3392     231      66
 
EcoRI G'AATT_C

Cuts at:    693     693 
   Size:       3689
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    177    1708     177 
   Size:       1531    2158
 
FspI TGC'GCA

Cuts at:   1470    1470 
   Size:       3689
 
HaeII r_GCGC'y

Cuts at:    146    1123    1371    2393    3372     146 
   Size:        977     248    1022     979     463

  Fragments arranged by size: 

                1022     979     977     463     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    188     211     220     243    1531    1561    1563    1593     188 
   Size:         23       9      23    1288      30       2      30    2284

  Fragments arranged by size: 

                2284    1288      30      30      23      23       9       2
            
 
HincII GTy'rAC

Cuts at:    261     728     261 
   Size:        467    3222
 
KasI G'GCGC_C

Cuts at:    142    1119    1367     142 
   Size:        977     248    2464

  Fragments arranged by size: 

                2464     977     248
 
KpnI G_GTAC'C

Cuts at:    402     709     402 
   Size:        307    3382
 
MluI A'CGCG_T

Cuts at:   3122    3122 
   Size:       3689
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2360    2544    2360 
   Size:        184    3505
 
MscI TGG'CCA

Cuts at:    538    1450     538 
   Size:        912    2777
 
MslI CAynn'nnrTG

Cuts at:   1678    1805    1678 
   Size:        127    3562
 
MspA1I CmG'CkG

Cuts at:    283     594     800    1114    1474    2487    2732     283 
   Size:        311     206     314     360    1013     245    1240

  Fragments arranged by size: 

                1240    1013     360     314     311     245     206
 
NaeI GCC'GGC

Cuts at:   1870    1870 
   Size:       3689
 
NarI GG'CG_CC

Cuts at:    143    1120    1368     143 
   Size:        977     248    2464

  Fragments arranged by size: 

                2464     977     248
 
NcoI C'CATG_G

Cuts at:    539     539 
   Size:       3689
 
NgoMIV G'CCGG_C

Cuts at:   1868    1868 
   Size:       3689
 
NspI r_CATG'y

Cuts at:    161     742    1242    2149     161 
   Size:        581     500     907    1701

  Fragments arranged by size: 

                1701     907     581     500
 
PciI A'CATG_T

Cuts at:   2145    2145 
   Size:       3689
 
PmeI GTTT'AAAC

Cuts at:    688     688 
   Size:       3689
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2828    2860    2828 
   Size:         32    3657
 
PstI C_TGCA'G

Cuts at:    736    1421     736 
   Size:        685    3004
 
PvuI CG_AT'CG

Cuts at:    104     303     104 
   Size:        199    3490
 
PvuII CAG'CTG

Cuts at:   1114    1474    1114 
   Size:        360    3329
 
RsrII CG'GwC_CG

Cuts at:   1884    1884 
   Size:       3689
 
SacI G_AGCT'C

Cuts at:    703     703 
   Size:       3689
 
SalI G'TCGA_C

Cuts at:    726     726 
   Size:       3689
 
SapI GCTCTTCn'nnn_

Cuts at:   1712    1922    1712 
   Size:        210    3479
 
SbfI CC_TGCA'GG

Cuts at:    736     736 
   Size:       3689
 
ScaI AGT'ACT

Cuts at:   3206    3206 
   Size:       3689
 
SfcI C'TryA_G

Cuts at:     33     732    1417    2410    2601      33 
   Size:        699     685     993     191    1121

  Fragments arranged by size: 

                1121     993     699     685     191
 
SfoI GGC'GCC

Cuts at:    144    1121    1369     144 
   Size:        977     248    2464

  Fragments arranged by size: 

                2464     977     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3689
 
SmaI CCC'GGG

Cuts at:    547     711     547 
   Size:        164    3525
 
SmlI C'TyrA_G

Cuts at:    844    1759    2251    2513     844 
   Size:        915     492     262    2020

  Fragments arranged by size: 

                2020     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3689
 
SphI G_CATG'C

Cuts at:    742    1242     742 
   Size:        500    3189
 
StyI C'CwwG_G

Cuts at:    539     823    1177     539 
   Size:        284     354    3051

  Fragments arranged by size: 

                3051     354     284
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    528    1272    1405    1864    2073     528 
   Size:        744     133     459     209    2144

  Fragments arranged by size: 

                2144     744     459     209     133
 
TatI w'GTAC_w

Cuts at:    200    3204     200 
   Size:       3004     685
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3206    3238    3206 
   Size:         32    3657
 
Tth111I GACn'n_nGTC

Cuts at:   1486    1486 
   Size:       3689
 
XbaI T'CTAG_A

Cuts at:    720     720 
   Size:       3689
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    123     240     441    1184     123 
   Size:        117     201     743    2628

  Fragments arranged by size: 

                2628     743     201     117
 
XmaI C'CCGG_G

Cuts at:    545     709     545 
   Size:        164    3525
 
ZraI GAC'GTC

Cuts at:    336     336 
   Size:       3689
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI
    BstYI     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI    BfrBI    BmgBI     BmtI   Bpu10I
    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII
   BstEII  BstZ17I   Bsu36I     ClaI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI
    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI
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