Restriction map for pF1KB6844
 (Linear) (Six-base) MAPSORT of: KIBB6844.seq  Check: 3564  from: 1  to: 576



KIBB6844.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AatII G_ACGT'C

Cuts at:      0     230     576 
   Size:        230     346
 
Acc65I G'GTAC_C

Cuts at:      0     290     576 
   Size:        290     286
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     514     576 
   Size:        514      62
 
AlwNI CAG_nnn'CTG

Cuts at:      0     426     576 
   Size:        426     150
 
ApaLI G'TGCA_C

Cuts at:      0     564     576 
   Size:        564      12
 
AvaI C'yCGr_G

Cuts at:      0     437     576 
   Size:        437     139
 
BanI G'GyrC_C

Cuts at:      0      34     290     305     340     402     576 
   Size:         34     256      15      35      62     174

  Fragments arranged by size: 

                 256     174      62      35      34      15
 
BanII G_rGCy'C

Cuts at:      0     259     576 
   Size:        259     317
 
BbeI G_GCGC'C

Cuts at:      0      38     576 
   Size:         38     538
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      52     502     564     576 
   Size:         52     450      62      12

  Fragments arranged by size: 

                 450      62      52      12
 
BclI T'GATC_A

Cuts at:      0      59     576 
   Size:         59     517
 
BlpI GC'TnA_GC

Cuts at:      0     471     576 
   Size:        471     105
 
Bme1580I G_kGCm'C

Cuts at:      0     343     568     576 
   Size:        343     225       8

  Fragments arranged by size: 

                 343     225       8
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     535     576 
   Size:        535      41
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     465     576 
   Size:        465     111
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     484     576 
   Size:        484      92
 
BsaHI Gr'CG_yC

Cuts at:      0      35     227     576 
   Size:         35     192     349

  Fragments arranged by size: 

                 349     192      35
 
BseYI C'CCAG_C

Cuts at:      0     482     576 
   Size:        482      94
 
BsiEI CG_ry'CG

Cuts at:      0     195     576 
   Size:        195     381
 
BsiHKAI G_wGCw'C

Cuts at:      0     472     530     568     576 
   Size:        472      58      38       8

  Fragments arranged by size: 

                 472      58      38       8
 
Bsp1286I G_dGCh'C

Cuts at:      0     259     343     472     530     568     576 
   Size:        259      84     129      58      38       8

  Fragments arranged by size: 

                 259     129      84      58      38       8
 
BsrBI CCG'CTC

Cuts at:      0     381     576 
   Size:        381     195
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     132     333     576 
   Size:        132     201     243

  Fragments arranged by size: 

                 243     201     132
 
BtgI C'CryG_G

Cuts at:      0      38     431     576 
   Size:         38     393     145

  Fragments arranged by size: 

                 393     145      38
 
BtsI GCAGTG_nn'

Cuts at:      0     309     576 
   Size:        309     267
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      90     125     576 
   Size:         90      35     451

  Fragments arranged by size: 

                 451      90      35
 
EaeI y'GGCC_r

Cuts at:      0     202     428     576 
   Size:        202     226     148

  Fragments arranged by size: 

                 226     202     148
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     484     514     576 
   Size:        484      30      62

  Fragments arranged by size: 

                 484      62      30
 
EcoO109I rG'GnC_Cy

Cuts at:      0     423     489     576 
   Size:        423      66      87

  Fragments arranged by size: 

                 423      87      66
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      69     576 
   Size:         69     507
 
HaeII r_GCGC'y

Cuts at:      0      38     576 
   Size:         38     538
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      80     103     112     135     576 
   Size:         80      23       9      23     441

  Fragments arranged by size: 

                 441      80      23      23       9
 
HincII GTy'rAC

Cuts at:      0     153     576 
   Size:        153     423
 
KasI G'GCGC_C

Cuts at:      0      34     576 
   Size:         34     542
 
KpnI G_GTAC'C

Cuts at:      0     294     576 
   Size:        294     282
 
MscI TGG'CCA

Cuts at:      0     430     576 
   Size:        430     146
 
MspA1I CmG'CkG

Cuts at:      0     175     486     576 
   Size:        175     311      90

  Fragments arranged by size: 

                 311     175      90
 
NarI GG'CG_CC

Cuts at:      0      35     576 
   Size:         35     541
 
NcoI C'CATG_G

Cuts at:      0     431     576 
   Size:        431     145
 
NspI r_CATG'y

Cuts at:      0      53     576 
   Size:         53     523
 
PvuI CG_AT'CG

Cuts at:      0     195     576 
   Size:        195     381
 
SfoI GGC'GCC

Cuts at:      0      36     576 
   Size:         36     540
 
SmaI CCC'GGG

Cuts at:      0     439     576 
   Size:        439     137
 
StyI C'CwwG_G

Cuts at:      0     431     576 
   Size:        431     145
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     420     576 
   Size:        420     156
 
TatI w'GTAC_w

Cuts at:      0      92     576 
   Size:         92     484
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      15     132     333     576 
   Size:         15     117     201     243

  Fragments arranged by size: 

                 243     201     117      15
 
XmaI C'CCGG_G

Cuts at:      0     437     576 
   Size:        437     139
 
ZraI GAC'GTC

Cuts at:      0     228     576 
   Size:        228     348
 

 Enzymes that do cut:

    AatII   Acc65I     AcuI    AlwNI    ApaLI     AvaI     BanI    BanII
     BbeI     BbsI     BclI     BlpI Bme1580I     BmrI     BplI     BpmI
    BsaHI    BseYI    BsiEI  BsiHKAI Bsp1286I    BsrBI    BstXI     BtgI
     BtsI    CspCI     EaeI  Eco57MI EcoO109I     FalI    HaeII    Hin4I
   HincII     KasI     KpnI     MscI   MspA1I     NarI     NcoI     NspI
     PvuI     SfoI     SmaI     StyI    TaqII     TatI     XcmI     XmaI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII    BmgBI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI     DraI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     MfeI     MluI
     MmeI     MslI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XhoI     XmnI
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