Restriction map for pF1KB5317
 (Linear) (Six-base) MAPSORT of: KIBB5317.seq  Check: 7195  from: 1  to: 1176



KIBB5317.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     927    1176 
   Size:        283     644     249

  Fragments arranged by size: 

                 644     283     249
 
AfeI AGC'GCT

Cuts at:      0     940    1176 
   Size:        940     236
 
AflIII A'CryG_T

Cuts at:      0     677    1176 
   Size:        677     499
 
AlwNI CAG_nnn'CTG

Cuts at:      0     541     759    1009    1023    1086    1176 
   Size:        541     218     250      14      63      90

  Fragments arranged by size: 

                 541     250     218      90      63      14
 
ApoI r'AATT_y

Cuts at:      0     141     193    1176 
   Size:        141      52     983

  Fragments arranged by size: 

                 983     141      52
 
AvaI C'yCGr_G

Cuts at:      0     112    1176 
   Size:        112    1064
 
BamHI G'GATC_C

Cuts at:      0     366    1176 
   Size:        366     810
 
BanI G'GyrC_C

Cuts at:      0     721    1128    1176 
   Size:        721     407      48

  Fragments arranged by size: 

                 721     407      48
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     362    1079    1176 
   Size:        362     717      97

  Fragments arranged by size: 

                 717     362      97
 
BbvCI CC'TCA_GC

Cuts at:      0     670    1176 
   Size:        670     506
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      35      69    1176 
   Size:         35      34    1107

  Fragments arranged by size: 

                1107      35      34
 
BlpI GC'TnA_GC

Cuts at:      0     889    1176 
   Size:        889     287
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      75    1176 
   Size:         75    1101
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     138    1176 
   Size:        138    1038
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     381     799    1176 
   Size:        381     418     377

  Fragments arranged by size: 

                 418     381     377
 
Bpu10I CC'TnA_GC

Cuts at:      0     670    1176 
   Size:        670     506
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     919    1176 
   Size:        157     762     257

  Fragments arranged by size: 

                 762     257     157
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     286    1176 
   Size:        286     890
 
BsaWI w'CCGG_w

Cuts at:      0     460    1176 
   Size:        460     716
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      85     298     301     304     322     487     520     568 
   Size:         85     213       3       3      18     165      33      48

Cuts at:    568     593     757     990    1049    1176 
   Size:         25     164     233      59     127

  Fragments arranged by size: 

                 233     213     165     164     127      85      59      48
                  33      25      18       3       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     772    1176 
   Size:        772     404
 
BsiHKAI G_wGCw'C

Cuts at:      0    1166    1176 
   Size:       1166      10
 
Bsp1286I G_dGCh'C

Cuts at:      0    1166    1176 
   Size:       1166      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1085    1176 
   Size:       1085      91
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     621    1176 
   Size:        621     555
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     994    1176 
   Size:        994     182
 
BstYI r'GATC_y

Cuts at:      0     275     366     410    1176 
   Size:        275      91      44     766

  Fragments arranged by size: 

                 766     275      91      44
 
Bsu36I CC'TnA_GG

Cuts at:      0     542    1002    1176 
   Size:        542     460     174

  Fragments arranged by size: 

                 542     460     174
 
BtgI C'CryG_G

Cuts at:      0     173    1176 
   Size:        173    1003
 
DraI TTT'AAA

Cuts at:      0    1157    1176 
   Size:       1157      19
 
EarI CTCTTCn'nnn_

Cuts at:      0      23     419     425     439     545     563    1176 
   Size:         23     396       6      14     106      18     613

  Fragments arranged by size: 

                 613     396     106      23      18      14       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     888     973    1176 
   Size:        888      85     203

  Fragments arranged by size: 

                 888     203      85
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     283     381     799     927    1176 
   Size:        283      98     418     128     249

  Fragments arranged by size: 

                 418     283     249     128      98
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     613    1176 
   Size:        613     563
 
EcoO109I rG'GnC_Cy

Cuts at:      0       6      39      63     117     180    1176 
   Size:          6      33      24      54      63     996

  Fragments arranged by size: 

                 996      63      54      33      24       6
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     822    1176 
   Size:        822     354
 
HaeII r_GCGC'y

Cuts at:      0     942    1176 
   Size:        942     234
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     986    1176 
   Size:        986     190
 
MspA1I CmG'CkG

Cuts at:      0     759    1176 
   Size:        759     417
 
NcoI C'CATG_G

Cuts at:      0     173    1176 
   Size:        173    1003
 
NspI r_CATG'y

Cuts at:      0     681    1176 
   Size:        681     495
 
PciI A'CATG_T

Cuts at:      0     677    1176 
   Size:        677     499
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     418     450    1176 
   Size:        418      32     726

  Fragments arranged by size: 

                 726     418      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      39      63     117     180    1176 
   Size:         39      24      54      63     996

  Fragments arranged by size: 

                 996      63      54      39      24
 
PstI C_TGCA'G

Cuts at:      0     617     899     974    1082    1176 
   Size:        617     282      75     108      94

  Fragments arranged by size: 

                 617     282     108      94      75
 
PvuII CAG'CTG

Cuts at:      0     759    1176 
   Size:        759     417
 
SbfI CC_TGCA'GG

Cuts at:      0     617    1176 
   Size:        617     559
 
SexAI A'CCwGG_T

Cuts at:      0     710    1176 
   Size:        710     466
 
SfcI C'TryA_G

Cuts at:      0     613     895     970    1078    1176 
   Size:        613     282      75     108      98

  Fragments arranged by size: 

                 613     282     108      98      75
 
SmlI C'TyrA_G

Cuts at:      0     112     136     934    1176 
   Size:        112      24     798     242

  Fragments arranged by size: 

                 798     242     112      24
 
StyI C'CwwG_G

Cuts at:      0     173    1176 
   Size:        173    1003
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     256     594     712    1176 
   Size:        256     338     118     464

  Fragments arranged by size: 

                 464     338     256     118
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1020    1052    1176 
   Size:       1020      32     124

  Fragments arranged by size: 

                1020     124      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     604    1176 
   Size:        604     572
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      88    1176 
   Size:         88    1088
 
XhoI C'TCGA_G

Cuts at:      0     112    1176 
   Size:        112    1064
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII    AlwNI     ApoI     AvaI    BamHI     BanI
     BbsI    BbvCI     BcgI     BlpI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaWI    BseRI     BsgI  BsiHKAI Bsp1286I    BspMI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI     DraI     EarI     EciI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII     MmeI   MspA1I     NcoI
     NspI     PciI     PpiI    PpuMI     PstI    PvuII     SbfI    SexAI
     SfcI     SmlI     StyI    TaqII     TstI  Tth111I     XcmI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BanII     BbeI    BciVI     BclI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmtI    BsaAI    BsaBI    BsaHI    BsaXI
    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     XbaI     XmaI     XmnI     ZraI
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