Restriction map for pF1KB7986
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       AccII       AciI        AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Aor51HI     ApaI        ApoI        AscI        Asp700I     
AspEI       AspI        AvaII       AvrII       BalI        
BamHI       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BfrI        BglI        BlnI        
BsaAI       BsaBI       BsaHI       BsgI        BsiEI       
BsiWI       BsmBI       BsmI        BspDI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoT22I     
FauI        FbaI        FseI        HaeII       HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        Ksp632I     KspI        MamI        MfeI        
MluI        MluNI       MscI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       Psp1406I    PstI        PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
Sse8387I    SspI        StuI        SwaI        Tth111I     
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   61 enzymes) #
###################################

Acc65I        764  GGTACC               G^GTACC
AccI          869  GTCTAC               GT^[AC][GT]AC
AccIII        164  TCCGGA               T^CCGGA
Alw44I        968  GTGCAC               G^TGCAC
AlwNI         529  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          700  CCTGAGG              CC^T[ACGT]AGG
ApaLI         968  GTGCAC               G^TGCAC
Asp718I       764  GGTACC               G^GTACC
AspHI         968  GTGCAC               G[AT]GC[AT]^C
AvaI           63  CCCGGG               C^[CT]CG[GA]G
AviII          17  TGCGCA               TGC^GCA
BanI          764  GGTACC               G^G[CT][GA]CC
BanII          67  GGGCTC               G[GA]GC[CT]^C
BbsI          880  GTCTTC               GAAGAC(2/6)
BfaI           34  CTAG                 C^TAG
BglII         888  AGATCT               A^GATCT
BlpI          308  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      308  GCTCAGC              GC^T[ACGT]AGC
BpuAI         880  GTCTTC               GAAGAC(2/6)
BsaI           24  GAGACC               GGTCTC(1/5)
BseAI         164  TCCGGA               T^CCGGA
BsiHKAI       968  GTGCAC               G[AT]GC[AT]^C
BsmFI         137  GGGAC                GGGAC(10/14)
BsoBI          63  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      626  TGTACA               T^GTACA
BspEI         164  TCCGGA               T^CCGGA
BspHI         710  TCATGA               T^CATGA
BsrGI         626  TGTACA               T^GTACA
BstXI         908  CCACCTGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        700  CCTGAGG              CC^T[ACGT]AGG
CelII         308  GCTCAGC              GC^T[ACGT]AGC
CfoI           18  GCGC                 GCG^C
DraII         530  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DsaI          109  CCATGG               C^C[GA][CT]GG
Eco81I        700  CCTGAGG              CC^T[ACGT]AGG
EcoO109I      530  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoRV         268  GATATC               GAT^ATC
EcoT14I       109  CCATGG               C^C[AT][AT]GG
FspI           17  TGCGCA               TGC^GCA
HhaI           18  GCGC                 GCG^C
HinP1I         18  GCGC                 G^CGC
KpnI          764  GGTACC               GGTAC^C
MaeI           34  CTAG                 C^TAG
MaeIII        642  GTCAC                ^GT[ACGT]AC
MroI          164  TCCGGA               T^CCGGA
MspA1I        311  CAGCTG               C[AC]G^C[GT]G
NcoI          109  CCATGG               C^CATGG
PflMI         221  CCAAGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI         930  GACCCCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         311  CAGCTG               CAG^CTG
RcaI          710  TCATGA               T^CATGA
SfcI          871  CTACAG               C^T[GA][CT]AG
SmaI           63  CCCGGG               CCC^GGG
SphI          117  GCATGC               GCATG^C
SspBI         626  TGTACA               T^GTACA
StyI          109  CCATGG               C^C[AT][AT]GG
TfiI          424  GATTC                G^A[AT]TC
Tsp45I        642  GTCAC                ^GT[GC]AC
Van91I        221  CCAAGACGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI          63  CCCGGG               C^CCGGG
XmaI           63  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwI          105  GATCC                GGATC(4/5)
              217  GATCC               
AseI           46  ATTAAT               AT^TAAT
               79  ATTAAT              
AsnI           46  ATTAAT               AT^TAAT
               79  ATTAAT              
BcnI           63  CCCGG                CC^[GC]GG
               64  CCGGG               
BmyI           67  GGGCTC               G[AGT]GC[ACT]^C
              968  GTGCAC              
BpmI          539  CTCCAG               CTGGAG(16/14)
              782  CTGGAG              
BsmAI          24  GAGAC                GTCTC(1/5)
              809  GAGAC               
Bsp1286I       67  GGGCTC               G[AGT]GC[ACT]^C
              968  GTGCAC              
Cfr13I        173  GGGCC                G^G[ACGT]CC
              531  GGCCC               
MaeII         226  ACGT                 A^CGT
              749  ACGT                
MseI           47  TTAA                 T^TAA
               80  TTAA                
MslI            9  CACTCGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              588  CATCCCAGTG          
MwoI           33  GCTAGAGATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              717  GCTCTTGGAGC         
NciI           63  CCCGG                CC^[GC]GG
               64  CCGGG               
NspI          117  GCATGC               [GA]CATG^[CT]
              506  ACATGC              
PshBI          46  ATTAAT               AT^TAAT
               79  ATTAAT              
Sau96I        173  GGGCC                G^G[ACGT]CC
              531  GGCCC               
TaqI          272  TCGA                 T^CGA
              373  TCGA                
Tru9I          47  TTAA                 T^TAA
               80  TTAA                
TthHB8I       272  TCGA                 T^CGA
              373  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AfaI        AluI        BbvI        BsaJI       BsaWI       
BseRI       BsiYI       BslI        BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       DdeI        
DpnII       EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HinfI       HpaII       HphI        ItaI        
MboI        MboII       MflI        MnlI        MspI        
MvaI        NdeII       NlaIII      NlaIV       PleI        
RsaI        Sau3AI      ScrFI       SfaNI       TseI        
Tsp509I     TspRI       XhoII       
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