Restriction map for
pF1KB7986
(Circular) (Six-base) MAPSORT of: pF1KB7986.seq Check: 533 from: 1 to: 4088
pF1KB7986 4088 bp
With 182 enzymes: SgfI *
October 7, 2009 14:25 ..
AccI GT'mk_AC
Cuts at: 978 1126 978
Size: 148 3940
Acc65I G'GTAC_C
Cuts at: 872 1104 872
Size: 232 3856
AclI AA'CG_TT
Cuts at: 3928 3928
Size: 4088
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 791 1021 1913 2345 3092 3360 791
Size: 230 892 432 747 268 1519
Fragments arranged by size:
1519 892 747 432 268 230
AflIII A'CryG_T
Cuts at: 1440 2544 3521 1440
Size: 1104 977 2007
Fragments arranged by size:
2007 1104 977
AgeI A'CCGG_T
Cuts at: 1080 2476 1080
Size: 1396 2692
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 878 910 878
Size: 32 4056
AlwNI CAG_nnn'CTG
Cuts at: 642 1393 2960 642
Size: 751 1567 1770
Fragments arranged by size:
1770 1567 751
ApaLI G'TGCA_C
Cuts at: 1076 2858 1076
Size: 1782 2306
ApoI r'AATT_y
Cuts at: 1092 3441 3697 1092
Size: 2349 256 1483
Fragments arranged by size:
2349 1483 256
AseI AT'TA_AT
Cuts at: 20 155 188 20
Size: 135 33 3920
Fragments arranged by size:
3920 135 33
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
AvaI C'yCGr_G
Cuts at: 171 1108 171
Size: 937 3151
BamHI G'GATC_C
Cuts at: 1113 1113
Size: 4088
BanI G'GyrC_C
Cuts at: 872 1104 1518 1766 1801 872
Size: 232 414 248 35 3159
Fragments arranged by size:
3159 414 248 232 35
BanII G_rGCy'C
Cuts at: 179 1102 2132 179
Size: 923 1030 2135
Fragments arranged by size:
2135 1030 923
BbeI G_GCGC'C
Cuts at: 1522 1770 1522
Size: 248 3840
BbsI GAAGACnn'nnnn_
Cuts at: 981 981
Size: 4088
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4054
BciVI GTATCCnnnnn_n'
Cuts at: 1986 2747 4088 1986
Size: 761 1341 1986
Fragments arranged by size:
1986 1341 761
BclI T'GATC_A
Cuts at: 1608 1608
Size: 4088
BglII A'GATC_T
Cuts at: 996 1603 3640 996
Size: 607 2037 1444
Fragments arranged by size:
2037 1444 607
BlpI GC'TnA_GC
Cuts at: 417 1200 417
Size: 783 3305
Bme1580I G_kGCm'C
Cuts at: 1080 1713 1806 2862 1080
Size: 633 93 1056 2306
Fragments arranged by size:
2306 1056 633 93
BmgBI CAC'GTC
Cuts at: 335 335
Size: 4088
BmrI ACTGGGnnnn_n'
Cuts at: 694 1036 1300 1381 1716 3860 694
Size: 342 264 81 335 2144 922
Fragments arranged by size:
2144 922 342 335 264 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 4088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 632 911 632
Size: 279 3809
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1264 2143 2635 2933 1264
Size: 879 492 298 2419
Fragments arranged by size:
2419 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 126 3792 126
Size: 3666 422
BsaAI yAC'GTr
Cuts at: 1441 1441
Size: 4088
BsaBI GATnn'nnATC
Cuts at: 6 1602 3639 6
Size: 1596 2037 455
Fragments arranged by size:
2037 1596 455
BsaHI Gr'CG_yC
Cuts at: 1519 1767 2469 3965 1519
Size: 248 702 1496 1642
Fragments arranged by size:
1642 1496 702 248
BsaWI w'CCGG_w
Cuts at: 272 434 1046 1080 1277 1798 2476 2750 2897
Size: 162 612 34 197 521 678 274 147
Cuts at: 2897 3308 272
Size: 411 1052
Fragments arranged by size:
1052 678 612 521 411 274 197 162
147 34
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 211 397 949 211
Size: 186 552 3350
Fragments arranged by size:
3350 552 186
BseYI C'CCAG_C
Cuts at: 242 278 1513 2271 2848 3344 242
Size: 36 1235 758 577 496 986
Fragments arranged by size:
1235 986 758 577 496 36
BsiEI CG_ry'CG
Cuts at: 104 1630 1676 2884 104
Size: 1526 46 1208 1308
Fragments arranged by size:
1526 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1080 1102 1880 2070 2862 1080
Size: 22 778 190 792 2306
Fragments arranged by size:
2306 792 778 190 22
Bsp1286I G_dGCh'C
Cuts at: 179 1080 1102 1713 1806 1880 2070 2132 2862
Size: 901 22 611 93 74 190 62 730
Cuts at: 2862 179
Size: 1405
Fragments arranged by size:
1405 901 730 611 190 93 74 62
22
BspEI T'CCGG_A
Cuts at: 272 3308 272
Size: 3036 1052
BspHI T'CATG_A
Cuts at: 818 3264 818
Size: 2446 1642
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1138 1352 1654 2035 1138
Size: 214 302 381 3191
Fragments arranged by size:
3191 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2380 2434 3918 4083 50
Size: 2330 54 1484 165 55
Fragments arranged by size:
2330 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2000 2000
Size: 4088
BsrFI r'CCGG_y
Cuts at: 1080 2086 2267 2476 1080
Size: 1006 181 209 2692
Fragments arranged by size:
2692 1006 209 181
BsrGI T'GTAC_A
Cuts at: 734 734
Size: 4088
BssSI C'ACGA_G
Cuts at: 2359 2717 2359
Size: 358 3730
BstAPI GCAn_nnn'nTGC
Cuts at: 1465 1465
Size: 4088
BstBI TT'CG_AA
Cuts at: 2449 2449
Size: 4088
BstXI CCAn_nnnn'nTGG
Cuts at: 1023 1023
Size: 4088
BstYI r'GATC_y
Cuts at: 1 212 324 996 1113 1580 1603 1938 2184
Size: 211 112 672 117 467 23 335 246
Cuts at: 2184 3196 3282 3294 3640 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 672 467 449 346 335 246 211
117 112 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 809 809
Size: 4088
BtgI C'CryG_G
Cuts at: 217 217
Size: 4088
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2038 2219 2382 2038
Size: 181 163 3744
Fragments arranged by size:
3744 181 163
BtsI GCAGTG_nn'
Cuts at: 1331 1331
Size: 4088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 839 874 839
Size: 35 4053
DraI TTT'AAA
Cuts at: 1087 1087
Size: 4088
DrdI GACnn_nn'nnGTC
Cuts at: 1794 2652 1794
Size: 858 3230
EaeI y'GGCC_r
Cuts at: 1673 1847 2238 2265 3610 1673
Size: 174 391 27 1345 2151
Fragments arranged by size:
2151 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1673 1673
Size: 4088
EarI CTCTTCn'nnn_
Cuts at: 2111 2321 2111
Size: 210 3878
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2296 2606 2752 3898 2296
Size: 310 146 1146 2486
Fragments arranged by size:
2486 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1100 1100
Size: 4088
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 632 791 911 1021 1913 2345 3092 3360 632
Size: 159 120 110 892 432 747 268 1360
Fragments arranged by size:
1360 892 747 432 268 159 120 110
EcoO109I rG'GnC_Cy
Cuts at: 639 1227 639
Size: 588 3500
EcoRI G'AATT_C
Cuts at: 1092 1092
Size: 4088
EcoRV GAT'ATC
Cuts at: 378 378
Size: 4088
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 363 2107 363
Size: 1744 2344
FspI TGC'GCA
Cuts at: 127 1869 127
Size: 1742 2346
FspAI rTGC'GCAy
Cuts at: 127 127
Size: 4088
HaeII r_GCGC'y
Cuts at: 1522 1770 2792 3771 1522
Size: 248 1022 979 1839
Fragments arranged by size:
1839 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 535 567 1930 1960 1962 1992 535
Size: 32 1363 30 2 30 2631
Fragments arranged by size:
2631 1363 32 30 30 2
HincII GTy'rAC
Cuts at: 1127 1127
Size: 4088
KasI G'GCGC_C
Cuts at: 1518 1766 1518
Size: 248 3840
KpnI G_GTAC'C
Cuts at: 876 1108 876
Size: 232 3856
MluI A'CGCG_T
Cuts at: 3521 3521
Size: 4088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 185 818 2759 2943 185
Size: 633 1941 184 1330
Fragments arranged by size:
1941 1330 633 184
MscI TGG'CCA
Cuts at: 1849 1849
Size: 4088
MslI CAynn'nnrTG
Cuts at: 121 700 2077 2204 121
Size: 579 1377 127 2005
Fragments arranged by size:
2005 1377 579 127
MspA1I CmG'CkG
Cuts at: 421 1199 1513 1873 2886 3131 421
Size: 778 314 360 1013 245 1378
Fragments arranged by size:
1378 1013 778 360 314 245
NaeI GCC'GGC
Cuts at: 2269 2269
Size: 4088
NarI GG'CG_CC
Cuts at: 1519 1767 1519
Size: 248 3840
NcoI C'CATG_G
Cuts at: 217 217
Size: 4088
NgoMIV G'CCGG_C
Cuts at: 2267 2267
Size: 4088
NspI r_CATG'y
Cuts at: 229 618 1141 1641 2548 229
Size: 389 523 500 907 1769
Fragments arranged by size:
1769 907 523 500 389
PasI CC'CwG_GG
Cuts at: 955 955
Size: 4088
PciI A'CATG_T
Cuts at: 2544 2544
Size: 4088
PflMI CCAn_nnn'nTGG
Cuts at: 335 335
Size: 4088
PmeI GTTT'AAAC
Cuts at: 1087 1087
Size: 4088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3227 3259 3227
Size: 32 4056
PshAI GACnn'nnGTC
Cuts at: 1042 1042
Size: 4088
PstI C_TGCA'G
Cuts at: 1135 1820 1135
Size: 685 3403
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4088
PvuII CAG'CTG
Cuts at: 421 1513 1873 421
Size: 1092 360 2636
Fragments arranged by size:
2636 1092 360
RsrII CG'GwC_CG
Cuts at: 2283 2283
Size: 4088
SacI G_AGCT'C
Cuts at: 1102 1102
Size: 4088
SalI G'TCGA_C
Cuts at: 1125 1125
Size: 4088
SapI GCTCTTCn'nnn_
Cuts at: 2111 2321 2111
Size: 210 3878
SbfI CC_TGCA'GG
Cuts at: 1135 1135
Size: 4088
ScaI AGT'ACT
Cuts at: 3605 3605
Size: 4088
SfcI C'TryA_G
Cuts at: 33 979 1131 1816 2809 3000 33
Size: 946 152 685 993 191 1121
Fragments arranged by size:
1121 993 946 685 191 152
SfoI GGC'GCC
Cuts at: 1520 1768 1520
Size: 248 3840
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4088
SmaI CCC'GGG
Cuts at: 173 1110 173
Size: 937 3151
SmlI C'TyrA_G
Cuts at: 1243 2158 2650 2912 1243
Size: 915 492 262 2419
Fragments arranged by size:
2419 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4088
SphI G_CATG'C
Cuts at: 229 1141 1641 229
Size: 912 500 2676
Fragments arranged by size:
2676 912 500
StyI C'CwwG_G
Cuts at: 217 1222 1576 217
Size: 1005 354 2729
Fragments arranged by size:
2729 1005 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 768 1671 1804 2263 2472 768
Size: 903 133 459 209 2384
Fragments arranged by size:
2384 903 459 209 133
TatI w'GTAC_w
Cuts at: 734 3603 734
Size: 2869 1219
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 510 542 3605 3637 510
Size: 32 3063 32 961
Fragments arranged by size:
3063 961 32 32
Tth111I GACn'n_nGTC
Cuts at: 1885 1885
Size: 4088
XbaI T'CTAG_A
Cuts at: 1119 1119
Size: 4088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1583 1583
Size: 4088
XmaI C'CCGG_G
Cuts at: 171 1108 171
Size: 937 3151
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BssHII BstEII BstZ17I
ClaI DraIII EcoNI FseI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PpuMI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI